##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921381_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3431039
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.415703523043604	33.0	31.0	34.0	29.0	34.0
2	31.547365681357746	34.0	31.0	34.0	29.0	34.0
3	31.625816844401943	34.0	31.0	34.0	28.0	34.0
4	35.120009711344	37.0	35.0	37.0	33.0	37.0
5	34.866291814228866	37.0	35.0	37.0	32.0	37.0
6	34.820387060595934	37.0	35.0	37.0	32.0	37.0
7	34.68647776956193	37.0	35.0	37.0	32.0	37.0
8	34.648933165726184	37.0	35.0	37.0	32.0	37.0
9	36.26800394865812	39.0	37.0	39.0	32.0	39.0
10-11	36.11608582706288	39.0	37.0	39.0	32.0	39.0
12-13	36.0568858296277	39.0	37.0	39.0	31.5	39.0
14-15	37.29938496764391	40.0	38.0	41.0	31.5	41.0
16-17	37.27362731813891	40.0	38.0	41.0	31.0	41.0
18-19	37.23867624355188	40.0	38.0	41.0	31.0	41.0
20-21	37.20160132251485	40.0	38.0	41.0	31.0	41.0
22-23	37.09555618575015	40.0	38.0	41.0	31.0	41.0
24-25	37.030915562312174	40.0	37.0	41.0	31.0	41.0
26-27	36.89748222040029	40.0	37.0	41.0	30.0	41.0
28-29	36.7474652721814	40.0	37.0	41.0	30.0	41.0
30-31	36.61613173152506	40.0	37.0	41.0	30.0	41.0
32-33	36.57435794230261	40.0	37.0	41.0	30.0	41.0
34-35	36.70912936285481	40.0	37.0	41.0	30.0	41.0
36-37	36.69091563809097	40.0	37.0	41.0	30.0	41.0
38-39	36.6088474948842	40.0	37.0	41.0	30.0	41.0
40-41	36.494891197680936	40.0	37.0	41.0	30.0	41.0
42-43	36.40233730948555	40.0	37.0	41.0	30.0	41.0
44-45	36.279412154743795	40.0	36.0	41.0	29.0	41.0
46-47	36.13843823984513	40.0	36.0	41.0	28.5	41.0
48-49	35.99331937060465	40.0	36.0	41.0	28.0	41.0
50-51	35.846977548200414	40.0	35.5	41.0	28.0	41.0
52-53	35.684651063424226	39.0	35.0	41.0	28.0	41.0
54-55	35.48238405334361	39.0	35.0	41.0	27.0	41.0
56-57	35.22527607526466	39.0	35.0	41.0	26.0	41.0
58-59	35.00596553988456	39.0	35.0	40.5	26.0	41.0
60-61	34.736829426887894	38.0	34.5	40.0	26.0	41.0
62-63	34.445870332572724	37.5	34.0	40.0	26.0	41.0
64-65	34.13557919335805	37.0	34.0	40.0	26.0	41.0
66-67	33.800063916498765	36.5	34.0	39.5	25.0	41.0
68-69	33.43103109582841	36.0	34.0	39.0	24.0	41.0
70-71	33.04751563593419	35.5	33.5	39.0	23.5	40.0
72-73	32.64623675219081	35.0	33.0	37.5	22.0	40.0
74-75	32.23583089553922	35.0	33.0	37.0	21.5	39.0
76-77	29.46404325337019	33.0	29.0	34.5	15.0	36.5
78-79	31.114906738162986	34.5	31.5	36.0	19.0	38.0
80-81	31.30618276271415	35.0	32.0	36.0	20.0	37.0
82-83	31.13523046517396	35.0	32.0	35.0	19.5	37.0
84-85	30.900358462844636	35.0	32.0	35.0	18.0	36.5
86-87	30.649202763361185	35.0	32.0	35.0	16.0	36.0
88-89	30.36769459630159	35.0	32.0	35.0	10.0	36.0
90-91	30.185721438899414	34.5	31.5	35.0	4.5	35.0
92-93	30.0397483094771	34.0	31.0	35.0	2.0	35.0
94-95	29.936664812029242	34.0	31.0	35.0	2.0	35.0
96-97	29.744778476723816	34.0	31.0	35.0	2.0	35.0
98-99	29.474199214873394	34.0	31.0	35.0	2.0	35.0
100-101	28.379641560471917	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61300.0
3	20455.0
4	11175.0
5	7907.0
6	7030.0
7	7086.0
8	7325.0
9	7888.0
10	8868.0
11	9704.0
12	10387.0
13	11155.0
14	11962.0
15	13102.0
16	14083.0
17	15003.0
18	14055.0
19	12761.0
20	12376.0
21	12308.0
22	13092.0
23	14618.0
24	17185.0
25	20868.0
26	25184.0
27	30383.0
28	36819.0
29	44709.0
30	53984.0
31	67331.0
32	85915.0
33	112667.0
34	161323.0
35	238546.0
36	414312.0
37	811127.0
38	888202.0
39	118841.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.30191074100693	20.039747990268513	14.030199831736622	25.628141436987935
2	14.927034881433322	21.015452242669312	38.04215244258054	26.015360433316825
3	17.50602337296061	24.827984826871386	29.529626017473465	28.136365782694543
4	13.030692892693796	17.24519454415936	35.84540113766332	33.87871142548352
5	14.213241774436607	37.33350553589447	34.497935755811326	13.955316933857597
6	33.21077402687037	35.97353185317832	17.10065193973055	13.715042180220763
7	28.619140108118945	32.001529567162805	21.219450918031754	18.159879406686493
8	26.351976813594728	35.499752840008206	19.797602781249417	18.350667565147642
9	26.37760748274794	15.804192257797128	19.598873693945187	38.219326565509746
10-11	25.403301632522002	25.641914805060363	28.026470948233516	20.92831261418412
12-13	25.90320571467044	22.906101930353838	27.907691832391972	23.283000522583748
14-15	23.20773173279609	24.6284300557763	24.918839307366575	27.244998904061035
16-17	23.53009497586657	27.68843870331048	25.72233785806855	23.0591284627544
18-19	23.534270546793344	27.125183108706384	26.874558076281645	22.465988268218624
20-21	24.004717559926213	26.23098556081575	26.82572279715804	22.9385740821
22-23	23.875971974604717	26.01311384245727	26.530145020549217	23.580769162388798
24-25	23.699148699032115	26.265730181195835	26.473017653244668	23.562103466527386
26-27	23.586111939356684	26.451414143092272	26.459414849602748	23.503059067948296
28-29	23.649639866072324	26.639971295383276	26.312132976293917	23.398255862250487
30-31	24.046399663722394	26.27132379283597	26.16217057635055	23.520105967091084
32-33	23.776098817211683	26.30544188150079	26.101550053046275	23.816909248241252
34-35	24.030215635245998	26.40013082229156	26.23386291875959	23.335790623702852
36-37	24.03153644497859	26.252920236857058	26.29621646891903	23.419326849245316
38-39	23.90976711678378	26.34374254051185	26.22692640962649	23.51956393307788
40-41	24.067409891072547	26.20040185242103	26.204803079343193	23.52738517716323
42-43	23.858290002224	26.175259483850922	26.058390096867946	23.90806041705713
44-45	23.815819120442285	26.21251290170916	26.181601266429734	23.790066711418824
46-47	23.959586573144218	26.186630304706792	26.00420352573235	23.84957959641664
48-49	23.725153835615238	26.286389301028095	26.1274671268375	23.860989736519162
50-51	23.837124401784177	26.253014214940013	26.144862448616617	23.764998934659197
52-53	24.05869865518074	26.13134171664872	26.058898889428576	23.751060738741963
54-55	23.626927239188237	26.03189032730605	26.341522275225742	23.99966015827997
56-57	23.635237748105304	26.156029749682762	26.183224362992412	24.025508139219514
58-59	23.767285590758654	26.098347237883452	26.231555757457308	23.902811413900587
60-61	23.787050789171733	26.148874869727166	26.305617628408463	23.758456712692634
62-63	23.75563213495465	26.118734798719633	26.29089997992961	23.83473308639611
64-65	23.566942027800263	25.933423803511484	26.28018727880836	24.21944688987989
66-67	23.563484400691763	26.1300458177608	26.090028714640447	24.216441066906995
68-69	23.578375130516743	26.040814077543452	26.19602750986082	24.184783282078985
70-71	23.60970688507717	25.880594904257094	26.126146352628677	24.383551858037062
72-73	23.566809347397818	26.113722349079232	25.988684176578154	24.330784126944792
74-75	23.500483572738887	26.14595806801212	26.020819500917952	24.332738858331044
76-77	23.530505686079557	25.873042493010544	26.113177569356203	24.48327425155369
78-79	23.416379863501138	25.918990258414514	26.13784051799568	24.526789360088667
80-81	23.557441001051995	26.020673963331014	25.981160287735378	24.440724747881607
82-83	23.52942891271061	26.04289513364919	26.06258704487963	24.36508890876057
84-85	23.492516134731588	26.03198797182054	25.985004718004994	24.490491175442873
86-87	23.391873166239602	26.13850477891615	25.962539621329984	24.507082433514267
88-89	23.285181122728158	25.95343612513097	25.98565383914608	24.775728912994786
90-91	23.257838231297427	25.86811862712663	26.111268594605765	24.762774546970178
92-93	23.37814449704815	25.783031343999557	26.14232744907347	24.696496709878822
94-95	23.27392214817057	25.76594784374254	26.090385117332705	24.869744890754177
96-97	23.127924343950067	25.673874364412463	26.186935316689038	25.011265974948437
98-99	22.951325668094924	25.6622219786901	26.108390967380522	25.278061385834455
100-101	22.937140592074005	25.68347619783211	26.12397587868225	25.255407331411632
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	27.5
2	34.0
3	47.0
4	45.0
5	52.0
6	84.0
7	198.5
8	310.0
9	326.5
10	285.5
11	239.5
12	237.0
13	269.5
14	295.5
15	341.0
16	400.0
17	471.5
18	561.5
19	618.0
20	703.5
21	841.5
22	973.5
23	1220.5
24	1618.0
25	2171.5
26	3050.0
27	4605.5
28	6948.5
29	10509.5
30	15776.0
31	22477.0
32	31280.5
33	38677.0
34	44925.0
35	52949.5
36	62914.5
37	73258.0
38	82720.0
39	91784.0
40	99290.0
41	107567.5
42	115944.0
43	123345.0
44	130891.5
45	138488.0
46	143962.5
47	148282.0
48	152624.0
49	154312.5
50	152931.0
51	150342.0
52	147479.0
53	144240.5
54	139676.0
55	131085.0
56	120718.5
57	108229.5
58	95195.0
59	82098.5
60	67126.5
61	54545.5
62	44243.5
63	33564.0
64	24027.0
65	17505.5
66	13017.5
67	9859.5
68	7383.5
69	5480.5
70	3976.5
71	2822.5
72	2031.0
73	1442.0
74	1041.5
75	762.0
76	513.0
77	305.5
78	166.0
79	88.5
80	54.5
81	31.5
82	24.5
83	19.0
84	10.5
85	9.0
86	6.0
87	3.0
88	3.0
89	3.0
90	2.0
91	1.0
92	1.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15904803180610888
2	4.663310443279718E-4
3	0.006907528594108082
4	0.0042844164697632406
5	0.028271319562383292
6	0.0013698474427134171
7	8.743707081149471E-5
8	0.001836178487041389
9	0.0
10-11	0.10884458031517566
12-13	2.914569027049824E-4
14-15	0.005566826841665163
16-17	2.7688405756973324E-4
18-19	4.954767345984701E-4
20-21	8.160793275739506E-4
22-23	2.331655221639859E-4
24-25	0.001603012964877403
26-27	0.0026814035048858377
28-29	0.006761800142755591
30-31	0.01573867274606905
32-33	0.01584068266201579
34-35	0.01256179250658474
36-37	7.140694116272068E-4
38-39	1.7487414162298942E-4
40-41	0.005173360023013437
42-43	0.008335667417362497
44-45	0.008510541558985485
46-47	0.010128127368998137
48-49	0.012503501126043745
50-51	0.006178886337345626
52-53	0.0016758771905536487
54-55	8.743707081149471E-4
56-57	0.006193459182480876
58-59	0.009545213563588173
60-61	0.0075341609349237944
62-63	0.01785173529068017
64-65	0.034377341674052674
66-67	0.036592414134610535
68-69	0.02691604496480512
70-71	0.041139141816808264
72-73	0.02583765442479669
74-75	0.024526098362624264
76-77	0.24429917584731622
78-79	0.0710863385697452
80-81	0.05381751708447499
82-83	0.020299973273402022
84-85	0.03187081231078982
86-87	0.0817099426733418
88-89	0.2625589508017834
90-91	0.31848953043086947
92-93	0.05345319595609376
94-95	0.00511506864247244
96-97	0.010346720046026874
98-99	0.0015155758940659085
100-101	0.0014281388232544137
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3431039.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.72067940693358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.34743112862316	47.573092479179344
2	12.20130206230069	14.817427017442352
3	3.905741244737232	7.114777859043815
4	1.757466241952893	4.268581769845199
5	0.9968627653299563	3.026509219315477
6	0.6175859305944591	2.2500142378715324
7	0.3940180704069215	1.6747531453602087
8	0.3144479784229975	1.5274795910384957
9	0.22134833684654903	1.2096379259025523
>10	1.178233442790864	12.495753456495432
>50	0.05119654784525575	2.114077928124434
>100	0.013068061474071383	1.3182131056951585
>500	0.0010577833665223093	0.4454303658244897
>1k	2.404053084724305E-4	0.16425189886147049
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.9145690270498237E-5	2.9145690270498237E-5
2	0.0	0.0	0.0	2.9145690270498237E-5	2.9145690270498237E-5
3	0.0	0.0	0.0	2.9145690270498237E-5	2.9145690270498237E-5
4	0.0	0.0	0.0	5.8291380540996475E-5	2.9145690270498237E-5
5	0.0	0.0	0.0	5.8291380540996475E-5	2.9145690270498237E-5
6	0.0	0.0	0.0	5.8291380540996475E-5	2.9145690270498237E-5
7	0.0	0.0	0.0	5.8291380540996475E-5	2.9145690270498237E-5
8	0.0	0.0	0.0	5.8291380540996475E-5	2.9145690270498237E-5
9	0.0	0.0	0.0	5.8291380540996475E-5	2.9145690270498237E-5
10-11	0.0	0.0	0.0	8.743707081149471E-5	2.9145690270498237E-5
12-13	0.0	0.0	0.0	8.743707081149471E-5	1.1658276108199295E-4
14-15	0.0	4.3718535405747356E-5	0.0	1.0200991594674383E-4	1.3115560621724208E-4
16-17	0.0	5.8291380540996475E-5	0.0	1.457284513524912E-4	1.457284513524912E-4
18-19	0.0	5.8291380540996475E-5	0.0	1.7487414162298942E-4	2.0401983189348766E-4
20-21	0.0	5.8291380540996475E-5	0.0	1.8944698675823856E-4	2.0401983189348766E-4
22-23	0.0	5.8291380540996475E-5	0.0	2.7688405756973324E-4	2.4773836729923503E-4
24-25	0.0	7.28642256762456E-5	0.0	4.954767345984701E-4	2.623112124344841E-4
26-27	0.0	8.743707081149471E-5	0.0	7.432151018977051E-4	2.623112124344841E-4
28-29	0.0	8.743707081149471E-5	0.0	0.0020984896994758733	2.623112124344841E-4
30-31	0.0	8.743707081149471E-5	0.0	0.010113554523862887	2.7688405756973324E-4
32-33	0.0	8.743707081149471E-5	0.0	0.02926227303158023	3.206025929754806E-4
34-35	0.0	8.743707081149471E-5	0.0	0.05329289465960603	3.4974828324597885E-4
36-37	0.0	8.743707081149471E-5	0.0	0.0925375666088319	3.4974828324597885E-4
38-39	0.0	1.0200991594674383E-4	0.0	0.15654150244284604	3.64321128381228E-4
40-41	0.0	1.1658276108199295E-4	0.0	0.22930371820314488	3.934668186517262E-4
42-43	0.0	1.1658276108199295E-4	0.0	0.3065106517296947	4.080396637869753E-4
44-45	0.0	1.1658276108199295E-4	0.0	0.40509594906965496	4.226125089222244E-4
46-47	0.0	1.1658276108199295E-4	0.0	0.5162576117613353	4.5175819919272267E-4
48-49	0.0	1.1658276108199295E-4	0.0	0.632417760334406	4.954767345984701E-4
50-51	0.0	1.1658276108199295E-4	0.0	0.7533578021118384	5.246224248689682E-4
52-53	0.0	1.1658276108199295E-4	0.0	0.8809284884258092	5.829138054099647E-4
54-55	0.0	1.7487414162298942E-4	0.0	1.0306790450356291	6.12059495680463E-4
56-57	0.0	2.0401983189348766E-4	0.0	1.202536607715622	6.557780310862103E-4
58-59	0.0	2.914569027049824E-4	0.0	1.382365516684596	6.703508762214594E-4
60-61	0.0	3.351754381107297E-4	0.0	1.5733135064917654	6.703508762214594E-4
62-63	0.0	3.4974828324597885E-4	0.0	1.772247415433051	8.160793275739506E-4
64-65	0.0	3.64321128381228E-4	0.0	1.9933029032896448	8.452250178444489E-4
66-67	0.0	3.7889397351647706E-4	0.0	2.250644775533009	8.452250178444489E-4
68-69	0.0	3.7889397351647706E-4	0.0	2.532002696559264	8.452250178444489E-4
70-71	0.0	3.934668186517262E-4	0.0	2.823430453573976	8.452250178444489E-4
72-73	0.0	4.080396637869753E-4	0.0	3.131616982494224	9.035163983854453E-4
74-75	0.0	4.080396637869753E-4	0.0	3.4661366425738676	9.472349337911927E-4
76-77	0.0	4.080396637869753E-4	0.0	3.8446808678070985	9.909534691969401E-4
78-79	0.0	4.080396637869753E-4	0.0	4.266287850414991	9.909534691969401E-4
80-81	2.9145690270498237E-5	4.080396637869753E-4	0.0	4.702103357029751	0.0010492448497379367
82-83	2.9145690270498237E-5	4.080396637869753E-4	0.0	5.149868596655415	0.0011366819205494312
84-85	2.9145690270498237E-5	4.080396637869753E-4	0.0	5.629941833945927	0.0011366819205494312
86-87	2.9145690270498237E-5	4.080396637869753E-4	0.0	6.154535113124625	0.0011804004559551786
88-89	2.9145690270498237E-5	4.080396637869753E-4	0.0	6.722278586748795	0.0012386918364961752
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2575	0.0	48.178997	1
GTATCAA	4465	0.0	40.134174	1
TCAACGC	5515	0.0	31.66833	4
CAACGCA	5680	0.0	30.722609	5
ATCAACG	5790	0.0	30.16422	3
TATCAAC	6030	0.0	29.278475	2
AACGCAG	6170	0.0	28.434776	6
GTACATG	8515	0.0	24.841045	1
TACATGG	8815	0.0	23.366316	2
ACGCAGA	7460	0.0	23.007565	7
ACATGGG	9105	0.0	22.413588	3
CGCAGAG	7675	0.0	21.929422	8
CATGGGG	7815	0.0	20.769209	4
GTATATA	1200	0.0	18.61706	1
GTATACG	205	1.5814278E-5	18.54943	1
GCAGAGT	8930	0.0	18.155373	9
AGAGTAC	7340	0.0	18.039787	10-11
ATGGGGG	4845	0.0	16.19312	5
GTATAGA	920	0.0	16.016525	1
CAGAGTA	8230	0.0	15.915639	10-11
>>END_MODULE