FastQCFastQC Report
Mon 15 Aug 2016
SRR2921380_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921380_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605413
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27250.057.808351
GTATCAA49100.041.6499751
TCAACGC61850.031.7629874
TATCAAC65100.030.831532
CAACGCA63300.030.808735
ATCAACG64350.030.6003823
AACGCAG67350.029.0914086
GTACATG85150.026.4126931
TACATGG89050.024.6174872
ACGCAGA81550.023.6213347
ACATGGG92300.023.287553
CGCAGAG82250.022.7302558
CATGGGG76300.021.5185134
GTGGTAT13500.020.7365861
ATGGGGG51950.019.7289455
GCAGAGT89000.019.5134969
ATATGTA14150.018.4427321
CAGAGTA83750.018.38578810-11
TACGGTA1300.007611775318.2499772
GTATAGC8400.018.0754741
Produced by FastQC (version 0.11.5)