##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921380_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2605413
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.17239416553153	31.0	31.0	34.0	26.0	34.0
2	30.22907385508555	31.0	31.0	34.0	26.0	34.0
3	30.265251228883866	31.0	31.0	34.0	26.0	34.0
4	33.74741048732005	37.0	35.0	37.0	28.0	37.0
5	33.73761587894126	37.0	35.0	37.0	28.0	37.0
6	33.778899544909	37.0	35.0	37.0	29.0	37.0
7	33.69670988822118	37.0	35.0	37.0	28.0	37.0
8	33.66042120769337	37.0	35.0	37.0	28.0	37.0
9	35.17709054188338	39.0	35.0	39.0	28.0	39.0
10-11	35.066163790539164	39.0	35.0	39.0	28.0	39.0
12-13	34.943363297872544	39.0	35.0	39.0	27.0	39.0
14-15	36.02105942512761	40.0	36.0	41.0	27.0	41.0
16-17	35.96241920186934	40.0	36.0	41.0	27.0	41.0
18-19	35.92310201875864	40.0	36.0	41.0	27.0	41.0
20-21	35.86330804367676	40.0	36.0	41.0	27.0	41.0
22-23	35.743920061809774	40.0	36.0	41.0	27.0	41.0
24-25	35.63605846750592	40.0	36.0	41.0	26.5	41.0
26-27	35.47612393889184	40.0	35.5	41.0	25.5	41.0
28-29	35.32974484275621	39.0	35.0	41.0	25.0	41.0
30-31	35.167975096462634	39.0	35.0	41.0	24.5	41.0
32-33	34.96921179099053	39.0	35.0	41.0	24.0	41.0
34-35	34.79582373312792	39.0	35.0	41.0	23.5	41.0
36-37	34.6111850597199	39.0	34.0	40.5	23.0	41.0
38-39	34.83160999810779	39.0	35.0	41.0	23.5	41.0
40-41	34.94199403319167	39.0	35.0	41.0	23.0	41.0
42-43	34.77026828376154	39.0	35.0	41.0	22.0	41.0
44-45	34.64055852181593	39.0	35.0	41.0	20.5	41.0
46-47	34.471639812958635	39.0	34.5	41.0	18.5	41.0
48-49	34.319053255664265	39.0	34.0	41.0	17.0	41.0
50-51	32.61151341457189	37.0	32.0	39.5	13.5	40.0
52-53	32.76367662247789	37.5	32.5	39.5	13.0	40.0
54-55	33.62106449150288	38.0	33.0	40.0	11.5	41.0
56-57	33.646283333966636	38.0	34.0	41.0	10.5	41.0
58-59	33.47151238594419	38.0	33.0	40.0	8.0	41.0
60-61	33.23132417010278	37.5	33.0	40.0	7.0	41.0
62-63	32.93937717359974	37.0	33.0	40.0	4.0	41.0
64-65	32.655450786497184	36.5	33.0	40.0	2.0	41.0
66-67	32.31957620538471	36.0	32.0	39.0	2.0	41.0
68-69	31.952960048944256	35.5	32.0	39.0	2.0	41.0
70-71	31.565729886202302	35.0	32.0	39.0	2.0	40.5
72-73	31.20742565574057	35.0	31.5	37.5	2.0	40.0
74-75	30.80538536500739	35.0	31.0	37.0	2.0	39.0
76-77	30.419580696035524	35.0	31.0	36.5	2.0	39.0
78-79	30.065779206597956	35.0	31.0	36.0	2.0	38.5
80-81	29.711660301073188	35.0	30.5	36.0	2.0	37.0
82-83	29.39978671327732	35.0	30.0	35.0	2.0	37.0
84-85	29.155600666765693	35.0	30.0	35.0	2.0	36.5
86-87	28.91462121360414	34.0	30.0	35.0	2.0	36.0
88-89	28.69612168972827	34.0	30.0	35.0	2.0	36.0
90-91	28.431379401269588	34.0	29.0	35.0	2.0	35.5
92-93	28.223411029268682	34.0	29.0	35.0	2.0	35.0
94-95	27.996250306573277	34.0	29.0	35.0	2.0	35.0
96-97	27.713717172670897	34.0	29.0	35.0	2.0	35.0
98-99	27.456915276004224	34.0	29.0	35.0	2.0	35.0
100-101	26.38775004193193	33.0	25.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94548.0
3	16196.0
4	11583.0
5	8722.0
6	7884.0
7	8154.0
8	8871.0
9	9375.0
10	10172.0
11	11097.0
12	11321.0
13	11221.0
14	12249.0
15	13105.0
16	13647.0
17	14688.0
18	13475.0
19	12293.0
20	12352.0
21	12978.0
22	14245.0
23	16211.0
24	18910.0
25	22161.0
26	26191.0
27	30956.0
28	35921.0
29	42264.0
30	50051.0
31	60513.0
32	74801.0
33	96981.0
34	135274.0
35	190247.0
36	303183.0
37	526543.0
38	547892.0
39	99123.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.672288201688644	18.51711901226262	13.464534484751505	24.346058301297226
2	16.142445177278788	19.89931997973803	39.14822920911113	24.810005633872052
3	19.08098007697028	23.879334378343255	29.938151899181374	27.10153364550509
4	13.770181932135097	16.029836347870035	36.31829943723698	33.881682282757886
5	14.349787838642166	37.379452028492985	34.103143060115585	14.167617072749266
6	33.16539548825075	34.94498128933884	17.32958864185322	14.560034580557188
7	28.491581938384503	32.447368400848525	20.951411020524425	18.10963864024255
8	26.701995582094046	34.43997245143106	20.150380789096047	18.70765117737885
9	27.13856073856473	15.612038531408503	19.722086150529247	37.527314579497514
10-11	25.55635621232445	25.395230969216133	27.80358690819969	21.244825910259728
12-13	25.997962505415757	23.038086022046176	27.60169764340543	23.36225382913264
14-15	23.45657632030492	24.815179678663675	25.0015311695236	26.726712831507804
16-17	23.33418183018852	27.698162409208916	26.01827301326957	22.949382747332994
18-19	23.322700264553415	27.095744072310495	27.406482038508628	22.175073624627462
20-21	23.861415047091388	26.17376216061193	27.433250168591446	22.531572623705234
22-23	23.890172740063527	25.845287357381157	26.99601097327951	23.2685289292758
24-25	23.65588858592456	26.08820447365804	26.920857193214058	23.33504974720334
26-27	23.23112021310334	26.4179565208377	27.04453116421289	23.306392101846072
28-29	23.337088505813096	26.854909003449905	26.748703332992303	23.059299157744693
30-31	23.967346637948207	26.268156484627426	26.61899279901699	23.145504078407377
32-33	23.58272944692597	26.337898603894338	26.47382222717824	23.605549722001452
34-35	23.9269561512352	26.490592183360057	26.556193075440067	23.026258589964677
36-37	23.861690649760092	26.35608973288703	26.717361825179236	23.064857792173648
38-39	23.759147958756746	26.447321652708823	26.61133813342873	23.1821922551057
40-41	23.898983978678924	26.217460919710305	26.58985429562207	23.293700805988692
42-43	23.675835884968688	26.263251834869443	26.346686464279667	23.714225815882198
44-45	23.51682404669696	26.277193313721796	26.64066494368718	23.565317695894056
46-47	23.72716504242014	26.1298063980186	26.361482666584624	23.78154589297664
48-49	23.44083804990872	26.438499407936305	26.445806462281947	23.674856079873027
50-51	23.633853775484187	26.316171010222327	26.597793552947387	23.452181661346106
52-53	23.884413342312204	26.094645791693573	26.477869408221512	23.543071457772715
54-55	23.18515352077765	26.008336712899904	26.86654471382907	23.93996505249337
56-57	23.363532496264767	26.18194154289779	26.541704480526512	23.912821480310928
58-59	23.45148408796547	26.17252516588958	26.681621809251737	23.69436893689321
60-61	23.66739991099524	26.096260530851456	26.848396895064774	23.387942663088527
62-63	23.554892979857918	26.0300901419621	26.84068125038342	23.574335627796565
64-65	23.222878330261132	25.84116287858027	26.875403256436957	24.060555534721644
66-67	23.314279033482226	26.003302437638943	26.658098096666006	24.02432043221282
68-69	23.24198596965412	25.953140136934515	26.807586739742504	23.99728715366886
70-71	23.236517793628515	25.77117259146752	26.732124661142407	24.26018495376156
72-73	23.161581127042258	26.08079373603493	26.628792620079167	24.128832516843644
74-75	23.127376974162363	26.110164700996357	26.69230946713479	24.070148857706492
76-77	23.061928982419225	25.664167542456077	26.780563512325077	24.493339962799617
78-79	22.926606173307494	25.69831134405305	26.94456608890835	24.430516393731107
80-81	23.211234371381597	25.871013456903057	26.67892003179002	24.238832139925325
82-83	23.15346411443866	25.892547000301196	26.820358892157326	24.133629993102815
84-85	23.127502050683866	25.9111046574805	26.71304946547591	24.248343826359726
86-87	22.90978250373279	26.076795614756954	26.67638865324517	24.33703322826509
88-89	22.76537991960548	25.686158981251346	26.92781709275147	24.620644006391704
90-91	22.957299561696598	25.433715699479215	27.04629750386733	24.562687234956858
92-93	23.08551288571941	25.316766401178192	27.103158084266365	24.494562628836032
94-95	22.910381588306038	25.232659724919664	27.17962717881915	24.677331507955152
96-97	22.789629791234876	25.047447493204405	27.26690318749037	24.89601952807035
98-99	22.54917765049925	25.016572413270392	27.213595419204147	25.220654517026208
100-101	22.714472906311066	24.97870762856562	27.354526497483295	24.95229296764002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	141.0
1	104.5
2	99.5
3	122.5
4	115.0
5	146.5
6	211.5
7	421.5
8	631.0
9	710.5
10	685.0
11	600.0
12	596.5
13	647.0
14	755.0
15	858.5
16	948.0
17	1050.5
18	1158.0
19	1325.5
20	1496.0
21	1645.0
22	1864.5
23	2170.0
24	2544.5
25	3103.5
26	4129.5
27	5736.0
28	8040.5
29	11411.0
30	15939.0
31	21580.5
32	28837.0
33	34268.5
34	38256.0
35	43702.5
36	50644.5
37	57835.0
38	64153.0
39	70287.5
40	75425.0
41	80142.0
42	84464.0
43	88688.0
44	93639.0
45	99812.0
46	104220.0
47	107581.0
48	111206.0
49	112725.0
50	113019.5
51	111840.0
52	108888.0
53	106482.0
54	103778.0
55	97930.5
56	89928.0
57	80648.5
58	71808.5
59	61507.0
60	50100.0
61	41140.5
62	33650.0
63	25762.5
64	18342.0
65	13382.0
66	10098.5
67	7678.0
68	5747.0
69	4388.0
70	3351.5
71	2396.5
72	1743.5
73	1320.0
74	983.5
75	715.5
76	527.0
77	356.0
78	224.5
79	165.0
80	109.5
81	91.0
82	83.0
83	72.5
84	62.5
85	42.0
86	34.5
87	29.0
88	33.5
89	35.5
90	28.0
91	21.0
92	14.0
93	8.5
94	8.0
95	7.5
96	5.0
97	4.5
98	4.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05066375273325189
2	0.05834007890495672
3	0.04955068543835468
4	0.056612905516323135
5	0.048475999774316006
6	0.01846156444295012
7	0.016081903329721623
8	0.021455331649915
9	0.02214620100536844
10-11	0.023297649931124164
12-13	0.02853674254331271
14-15	0.045846858060507104
16-17	0.030973976102828992
18-19	0.024794533534606603
20-21	0.02853674254331271
22-23	0.04870628955946716
24-25	0.04398534896386869
26-27	0.03281629438403816
28-29	0.021551285727061314
30-31	0.013510334062200504
32-33	0.010497376039806357
34-35	0.010190322992938164
36-37	0.0013817387109068696
38-39	0.007177364970544017
40-41	0.00397249879385725
42-43	0.22157715494625996
44-45	0.19469082252986378
46-47	0.20085107428265692
48-49	0.19904713763230628
50-51	0.1966674765190778
52-53	0.20088945591351542
54-55	0.20063997531293504
56-57	0.21134845032246327
58-59	0.20785572191433757
60-61	0.21309481452652612
62-63	0.20950613204125412
64-65	0.21054243607443426
66-67	0.2000642508500572
68-69	0.1992774274174574
70-71	0.18281170777915057
72-73	0.19004664519598236
74-75	0.18972040133368492
76-77	0.18814675446848542
78-79	0.2158391011329106
80-81	0.22472444867665894
82-83	0.22269022224115717
84-85	0.21764303778326125
86-87	0.23604702977992356
88-89	0.23437742883757776
90-91	0.2330532625729587
92-93	0.23716009707482077
94-95	0.24946140976497777
96-97	0.24900083019467548
98-99	0.23850345415486912
100-101	0.24861701388609023
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2605413.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.06846562230265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.56447253728439	47.192473177402086
2	11.86639301313212	14.255920415401183
3	3.8961398165864622	7.021054218969254
4	1.778093829012561	4.2722947216507805
5	0.9988991288352058	3.0001168990292815
6	0.6069064404556641	2.1873563192679075
7	0.43510667351896964	1.8295333182216083
8	0.30679451687042064	1.4742940711793449
9	0.24002844029864945	1.2976326103008913
>10	1.240031523869416	12.848424803355272
>50	0.05012248299032724	2.014456019683157
>100	0.015213033146514076	1.6458017522404962
>500	0.0011562197139052062	0.4909123191348841
>1k	6.423442854851965E-4	0.4697293541638819
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.8381630858524156E-5	0.0
5	0.0	0.0	0.0	3.8381630858524156E-5	0.0
6	0.0	0.0	0.0	3.8381630858524156E-5	0.0
7	0.0	0.0	0.0	3.8381630858524156E-5	0.0
8	0.0	0.0	0.0	3.8381630858524156E-5	0.0
9	0.0	0.0	0.0	3.8381630858524156E-5	7.676326171704831E-5
10-11	0.0	0.0	0.0	7.676326171704831E-5	7.676326171704831E-5
12-13	0.0	1.9190815429262078E-5	0.0	7.676326171704831E-5	1.5352652343409662E-4
14-15	0.0	5.757244628778624E-5	0.0	7.676326171704831E-5	1.727173388633587E-4
16-17	0.0	7.676326171704831E-5	0.0	7.676326171704831E-5	2.3028978515114495E-4
18-19	0.0	7.676326171704831E-5	0.0	7.676326171704831E-5	2.3028978515114495E-4
20-21	0.0	7.676326171704831E-5	0.0	9.59540771463104E-5	2.49480600580407E-4
22-23	0.0	7.676326171704831E-5	0.0	1.727173388633587E-4	3.0705304686819325E-4
24-25	0.0	7.676326171704831E-5	0.0	4.79770385731552E-4	3.8381630858524155E-4
26-27	0.0	7.676326171704831E-5	0.0	0.0012665938183312971	4.030071240145037E-4
28-29	0.0	7.676326171704831E-5	0.0	0.004164406948149871	4.2219793944376575E-4
30-31	0.0	7.676326171704831E-5	0.0	0.014700164618814751	4.2219793944376575E-4
32-33	0.0	7.676326171704831E-5	0.0	0.04264199188382034	4.2219793944376575E-4
34-35	0.0	7.676326171704831E-5	0.0	0.08286594102355366	4.2219793944376575E-4
36-37	0.0	7.676326171704831E-5	0.0	0.14272209434742208	4.2219793944376575E-4
38-39	1.9190815429262078E-5	9.59540771463104E-5	0.0	0.22881209236309177	4.2219793944376575E-4
40-41	3.8381630858524156E-5	1.1514489257557247E-4	0.0	0.32351876650650013	4.2219793944376575E-4
42-43	3.8381630858524156E-5	1.1514489257557247E-4	0.0	0.4308146155715044	4.413887548730278E-4
44-45	3.8381630858524156E-5	1.1514489257557247E-4	0.0	0.5701399355879471	4.605795703022899E-4
46-47	3.8381630858524156E-5	1.1514489257557247E-4	0.0	0.7322447535189238	5.181520165900761E-4
48-49	3.8381630858524156E-5	1.1514489257557247E-4	0.0	0.8903770726560434	5.373428320193382E-4
50-51	3.8381630858524156E-5	1.1514489257557247E-4	0.0	1.0483750560851581	5.373428320193382E-4
52-53	3.8381630858524156E-5	1.1514489257557247E-4	0.0	1.219058168513015	5.373428320193382E-4
54-55	3.8381630858524156E-5	1.1514489257557247E-4	0.0	1.4400212173655387	5.373428320193382E-4
56-57	3.8381630858524156E-5	1.1514489257557247E-4	0.0	1.6898088709928138	5.373428320193382E-4
58-59	3.8381630858524156E-5	1.1514489257557247E-4	0.0	1.9390975634189282	5.373428320193382E-4
60-61	3.8381630858524156E-5	1.1514489257557247E-4	0.0	2.1913224506057194	5.757244628778623E-4
62-63	3.8381630858524156E-5	1.1514489257557247E-4	0.0	2.4636976939932365	6.141060937363865E-4
64-65	3.8381630858524156E-5	1.1514489257557247E-4	0.0	2.7713648469551657	6.141060937363865E-4
66-67	3.8381630858524156E-5	1.5352652343409662E-4	0.0	3.1443959172691622	6.141060937363865E-4
68-69	3.8381630858524156E-5	1.5352652343409662E-4	0.0	3.5402064854976927	6.141060937363865E-4
70-71	3.8381630858524156E-5	1.5352652343409662E-4	0.0	3.9478769776615072	6.908693554534348E-4
72-73	3.8381630858524156E-5	1.5352652343409662E-4	0.0	4.365545884663967	9.211591406045798E-4
74-75	3.8381630858524156E-5	1.5352652343409662E-4	0.0	4.827526384492593	9.211591406045798E-4
76-77	3.8381630858524156E-5	1.5352652343409662E-4	0.0	5.361338106472947	9.403499560338419E-4
78-79	3.8381630858524156E-5	1.5352652343409662E-4	0.0	5.949843652426698	9.59540771463104E-4
80-81	3.8381630858524156E-5	1.5352652343409662E-4	0.0	6.535662484220352	0.0010171132177508903
82-83	3.8381630858524156E-5	1.727173388633587E-4	0.0	7.137563219343727	0.0011130672948972005
84-85	3.8381630858524156E-5	1.9190815429262077E-4	0.0	7.785138095188747	0.0011130672948972005
86-87	3.8381630858524156E-5	2.3028978515114495E-4	0.0	8.50594512271183	0.0011130672948972005
88-89	3.8381630858524156E-5	2.3028978515114495E-4	0.0	9.284458932230706	0.0011130672948972005
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2725	0.0	57.80835	1
GTATCAA	4910	0.0	41.649975	1
TCAACGC	6185	0.0	31.762987	4
TATCAAC	6510	0.0	30.83153	2
CAACGCA	6330	0.0	30.80873	5
ATCAACG	6435	0.0	30.600382	3
AACGCAG	6735	0.0	29.091408	6
GTACATG	8515	0.0	26.412693	1
TACATGG	8905	0.0	24.617487	2
ACGCAGA	8155	0.0	23.621334	7
ACATGGG	9230	0.0	23.28755	3
CGCAGAG	8225	0.0	22.730255	8
CATGGGG	7630	0.0	21.518513	4
GTGGTAT	1350	0.0	20.736586	1
ATGGGGG	5195	0.0	19.728945	5
GCAGAGT	8900	0.0	19.513496	9
ATATGTA	1415	0.0	18.442732	1
CAGAGTA	8375	0.0	18.385788	10-11
TACGGTA	130	0.0076117753	18.249977	2
GTATAGC	840	0.0	18.075474	1
>>END_MODULE