FastQCFastQC Report
Mon 15 Aug 2016
SRR2921380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605413
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.057.2067831
GTATCAA48700.040.113311
TCAACGC63450.029.8434754
TATCAAC65700.029.0381432
CAACGCA65500.029.0354125
ATCAACG65850.028.6837183
AACGCAG69000.027.4910496
GTACATG88850.027.3362621
TACATGG88900.026.2112582
ACATGGG92100.024.4245663
ACCGTAT1951.5577825E-824.3794888
TACCGTA1801.9371873E-723.777
ACGCAGA82650.022.8928267
CATGGGG75050.022.195484
CGCAGAG84800.021.9199778
TACACCG3404.1836756E-1119.5775535
GTACCGT2752.8134309E-819.0163676
ATGGGGG50900.018.681925
GCAGAGT93500.018.6600869
AGAGTAC80000.018.60002510-11
Produced by FastQC (version 0.11.5)