##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921380_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2605413
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.478645420131087	33.0	31.0	34.0	30.0	34.0
2	31.609899083177982	34.0	31.0	34.0	29.0	34.0
3	31.67677677205111	34.0	31.0	34.0	28.0	34.0
4	35.19116124775611	37.0	35.0	37.0	33.0	37.0
5	34.94414167734636	37.0	35.0	37.0	32.0	37.0
6	34.902508738537804	37.0	35.0	37.0	32.0	37.0
7	34.770318179881656	37.0	35.0	37.0	32.0	37.0
8	34.73256063434089	37.0	35.0	37.0	32.0	37.0
9	36.35025195621577	39.0	37.0	39.0	32.0	39.0
10-11	36.2106639139361	39.0	37.0	39.0	32.0	39.0
12-13	36.14461929836076	39.0	37.0	39.0	32.0	39.0
14-15	37.37363308619401	40.0	38.0	41.0	32.0	41.0
16-17	37.34988848217154	40.0	38.0	41.0	32.0	41.0
18-19	37.32201746901547	40.0	38.0	41.0	31.5	41.0
20-21	37.28360167850548	40.0	38.0	41.0	31.0	41.0
22-23	37.17756685792233	40.0	37.5	41.0	31.0	41.0
24-25	37.1036430692562	40.0	37.0	41.0	31.0	41.0
26-27	36.96488119158076	40.0	37.0	41.0	30.5	41.0
28-29	36.809928790560264	40.0	37.0	41.0	30.0	41.0
30-31	36.66214204811291	40.0	37.0	41.0	30.0	41.0
32-33	36.60233828571516	40.0	36.5	41.0	30.0	41.0
34-35	36.720609362124165	40.0	37.0	41.0	30.0	41.0
36-37	36.6891707763798	40.0	37.0	41.0	30.0	41.0
38-39	36.59399277580944	40.0	37.0	41.0	30.0	41.0
40-41	36.46406212757824	40.0	36.5	41.0	30.0	41.0
42-43	36.35357676498889	40.0	36.0	41.0	29.5	41.0
44-45	36.20541004439603	40.0	36.0	41.0	29.0	41.0
46-47	36.03666750722438	40.0	36.0	41.0	28.0	41.0
48-49	35.870073381840044	40.0	35.0	41.0	28.0	41.0
50-51	35.71798252330821	39.5	35.0	41.0	27.5	41.0
52-53	35.547061060952714	39.0	35.0	41.0	27.0	41.0
54-55	35.34239753927688	39.0	35.0	41.0	26.5	41.0
56-57	35.0805317621429	39.0	35.0	41.0	26.0	41.0
58-59	34.85470019532412	38.5	35.0	40.5	26.0	41.0
60-61	34.58298972178308	38.0	34.0	40.0	26.0	41.0
62-63	34.28977977771662	37.5	34.0	40.0	25.0	41.0
64-65	33.98312705125828	37.0	34.0	40.0	25.0	41.0
66-67	33.65197302692509	36.5	34.0	39.5	23.5	41.0
68-69	33.28474621873768	36.0	34.0	39.0	23.0	41.0
70-71	32.90711894812838	35.5	33.0	39.0	22.5	40.0
72-73	32.50722476628465	35.0	33.0	37.5	21.5	40.0
74-75	32.09984578260721	35.0	33.0	37.0	20.5	39.0
76-77	29.320785226756755	33.0	29.0	34.5	13.0	36.5
78-79	30.9871580820392	34.5	31.5	36.0	17.5	38.0
80-81	31.186074722126587	35.0	32.0	36.0	18.0	37.0
82-83	31.019517251199716	35.0	32.0	35.0	18.0	37.0
84-85	30.7884039881585	35.0	32.0	35.0	16.0	36.5
86-87	30.541784738158594	35.0	32.0	35.0	11.0	36.0
88-89	30.25943737134957	35.0	32.0	35.0	7.0	36.0
90-91	30.072078016038148	34.5	31.0	35.0	2.0	35.0
92-93	29.921886856325656	34.0	31.0	35.0	2.0	35.0
94-95	29.82278126346955	34.0	31.0	35.0	2.0	35.0
96-97	29.62771583622251	34.0	31.0	35.0	2.0	35.0
98-99	29.361466493028168	34.0	31.0	35.0	2.0	35.0
100-101	28.26865184137793	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40735.0
3	14364.0
4	8002.0
5	5965.0
6	5350.0
7	5441.0
8	5765.0
9	6246.0
10	6947.0
11	7917.0
12	8324.0
13	9276.0
14	9910.0
15	11310.0
16	12441.0
17	13461.0
18	12225.0
19	10507.0
20	9982.0
21	9796.0
22	10673.0
23	11744.0
24	13776.0
25	16426.0
26	20041.0
27	23974.0
28	28857.0
29	34609.0
30	42454.0
31	52278.0
32	66430.0
33	87576.0
34	127500.0
35	186677.0
36	315301.0
37	605044.0
38	657474.0
39	90614.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.5140815806398	20.064642687315217	14.178221764857613	25.24305396718738
2	15.16670153008841	21.23378131231037	37.902207142459616	25.6973100151416
3	17.75328607465888	24.95869855936276	29.81948660313308	27.468528762845285
4	13.385785366067108	17.19574820721291	35.385546622060176	34.032919804659805
5	14.096149637768793	37.72065005816431	34.1344267456012	14.04877355846569
6	33.12357530543494	35.52901819041577	17.101785035060104	14.24562146908919
7	28.26155125740335	32.65798191378014	20.973520856034504	18.10694597278201
8	26.604917232421677	35.060958758992975	19.880761566120412	18.45336244246494
9	26.861844935908437	15.747867996359885	19.702788003283935	37.68749906444775
10-11	25.46951114437596	25.76439943358395	27.79071422712085	20.975375194919238
12-13	25.965621871289567	23.217471054900646	27.665548543000874	23.151358530808917
14-15	23.28209927164813	24.999620959936713	25.03867648038175	26.67960328803341
16-17	23.26465958729736	27.938912517065447	25.991830079783956	22.804597815853235
18-19	23.2765405321588	27.353178860957012	27.400676247641204	21.969604359242982
20-21	23.806986037060764	26.250759481184634	27.47295325817908	22.469301223575524
22-23	23.785765525309483	25.963275603389413	27.02153636648708	23.229422504814025
24-25	23.52218797875997	26.241194366928074	26.979958842595288	23.256658811716676
26-27	23.180100378607595	26.56563299321086	27.114314621991177	23.139952006190374
28-29	23.30839564237505	26.95819346225751	26.774159559639056	22.95925133572839
30-31	23.909677456504276	26.38482378581937	26.6335188421486	23.07197991552775
32-33	23.54348885511175	26.43957245794455	26.507787861228294	23.509150825715402
34-35	23.804773038638576	26.623734065335913	26.608686606341198	22.962806289684316
36-37	23.784461378940687	26.432194537611043	26.824975142957978	22.958368940490292
38-39	23.731158421253024	26.522487073041916	26.66071878185681	23.08563572384824
40-41	23.891090938785915	26.331889403592594	26.57305401023961	23.203965647381878
42-43	23.642670606481616	26.396261167815045	26.297516292904362	23.663551932798978
44-45	23.486366434975135	26.330710106142053	26.598061253161	23.584862205721812
46-47	23.654571933504172	26.292859237604198	26.36151155631371	23.69105727257792
48-49	23.368838171104194	26.546807769862934	26.483048001010328	23.60130605802254
50-51	23.59650467929333	26.443520201193394	26.52781139040351	23.432163729109767
52-53	23.818850717653763	26.214714636912678	26.4159532349594	23.550481410474156
54-55	23.088848080255303	26.13793620944002	26.79066111329782	23.982554597006853
56-57	23.32479801385892	26.237221490580758	26.46380217199635	23.97417832356397
58-59	23.422264949284433	26.262102633363355	26.541643307635038	23.77398910971717
60-61	23.584042008049238	26.230656586884383	26.694917693933128	23.490383711133248
62-63	23.475181102984884	26.186605797713998	26.694179771092656	23.644033328208454
64-65	23.129780737581	25.99402386805284	26.70031320460237	24.175882189763787
66-67	23.189289453748778	26.258292982693927	26.467875694817465	24.084541868739826
68-69	23.14919520711964	26.1640467939488	26.546829940894256	24.139928058037306
70-71	23.123810525794394	25.983809039821576	26.510004686381983	24.382375748002048
72-73	23.089581900454572	26.29328605561262	26.33739776612002	24.27973427781279
74-75	23.01067091166414	26.346388952820217	26.38936744073374	24.253572694781905
76-77	22.967736052643577	25.851228839390885	26.53445496095919	24.646580147006343
78-79	22.812979199213405	25.94735614061535	26.66510474328605	24.574559916885192
80-81	23.1533386417709	26.109544415858416	26.309215656268236	24.427901286102447
82-83	23.028077028278574	26.132030653914995	26.484656247150788	24.355236070655643
84-85	22.977389020760405	26.145325717313327	26.356893915470625	24.520391346455643
86-87	22.735602006255384	26.363302005199056	26.278046714675185	24.623049273870375
88-89	22.624668502126994	26.021257809188448	26.43145606237769	24.92261762630687
90-91	22.70520733748415	25.792293277592087	26.546324770265656	24.956174614658106
92-93	22.827699398365098	25.677160876181947	26.552405829180543	24.94273389627241
94-95	22.6257641018791	25.718501902589928	26.57071863528636	25.08501536024461
96-97	22.404104353073244	25.525956125523557	26.69161481557684	25.378324705826355
98-99	22.144197775372497	25.538788967444287	26.61625175598185	25.70076150120136
100-101	22.129915380881986	25.624702418706214	26.614711363656863	25.630670836754938
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	137.0
1	101.5
2	81.5
3	102.5
4	100.0
5	116.0
6	163.0
7	362.5
8	560.0
9	578.0
10	491.5
11	392.5
12	390.0
13	434.5
14	465.0
15	529.0
16	607.5
17	677.0
18	751.5
19	873.0
20	1048.5
21	1194.5
22	1360.5
23	1638.0
24	2088.0
25	2714.0
26	3755.5
27	5472.0
28	8023.5
29	11728.5
30	16392.0
31	22056.5
32	29612.0
33	35013.0
34	38656.0
35	44087.5
36	51271.0
37	58397.0
38	64557.0
39	70288.0
40	75468.0
41	80289.5
42	84891.5
43	89786.0
44	94621.5
45	100638.0
46	105527.0
47	109208.0
48	112641.5
49	113975.0
50	114020.5
51	112975.0
52	110123.0
53	107438.5
54	104911.0
55	98555.0
56	89606.0
57	80319.5
58	71006.0
59	60577.5
60	49045.0
61	40079.0
62	32931.5
63	24798.0
64	17424.5
65	12438.0
66	9142.5
67	6976.5
68	5219.0
69	3898.0
70	2915.5
71	2083.0
72	1458.5
73	1060.5
74	771.0
75	526.0
76	355.0
77	213.5
78	108.0
79	61.5
80	41.5
81	30.0
82	18.5
83	11.5
84	6.5
85	5.0
86	4.0
87	3.5
88	3.5
89	3.0
90	1.5
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	1.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1549082621450035
2	3.0705304686819325E-4
3	0.006793548661958776
4	0.004298742656154706
5	0.027749919110712964
6	0.0011514489257557247
7	1.5352652343409662E-4
8	0.0017271733886335872
9	0.0
10-11	0.10487780632091727
12-13	2.49480600580407E-4
14-15	0.005661290551632313
16-17	7.676326171704831E-5
18-19	2.49480600580407E-4
20-21	6.141060937363865E-4
22-23	2.3028978515114495E-4
24-25	0.0018998907274969457
26-27	0.00295538557610636
28-29	0.007158174155114756
30-31	0.015947567621716785
32-33	0.016484910453736124
34-35	0.012358885136444779
36-37	5.565336474486003E-4
38-39	1.9190815429262077E-4
40-41	0.005315855873905596
42-43	0.008232859819153431
44-45	0.008635866943167935
46-47	0.009864079130640708
48-49	0.012358885136444779
50-51	0.006083488491076079
52-53	0.0018423182812091596
54-55	0.0010554948486094144
56-57	0.006141060937363865
58-59	0.009461072006626204
60-61	0.007599562909987783
62-63	0.018135320580652665
64-65	0.03398693412522314
66-67	0.036539312577315
68-69	0.025888410014074544
70-71	0.04062695626374782
72-73	0.02542783044377225
74-75	0.023854183578572764
76-77	0.24078716119095128
78-79	0.06853040189789487
80-81	0.053062604661909644
82-83	0.020169547016154442
84-85	0.03130021996512645
86-87	0.07889344222969641
88-89	0.2606688459756668
90-91	0.31373145063757646
92-93	0.05150814861213941
94-95	0.005699672182490837
96-97	0.010612520932381929
98-99	0.0012665938183312971
100-101	0.0012474030029020352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2605413.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.695926619871365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.46249937074761	45.46733178857234
2	12.564519519549714	14.74972231466863
3	3.987524762771118	7.021543826116005
4	1.833646777863861	4.305103868810431
5	1.0381103767145907	3.046642524748332
6	0.6748713238785097	2.3767318622537066
7	0.45354453218192065	1.8634851605855731
8	0.34013956492583086	1.597184555472123
9	0.25245201742993506	1.3336114581095362
>10	1.322943106648337	13.495537329645297
>50	0.05139803656148173	2.0210819617240925
>100	0.016514822544228522	1.751974369773648
>500	0.001180149546201886	0.4860442976695466
>1k	6.556386367769166E-4	0.4840046818507625
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.8381630858524156E-5	0.0
5	0.0	0.0	0.0	3.8381630858524156E-5	0.0
6	0.0	0.0	0.0	3.8381630858524156E-5	0.0
7	0.0	0.0	0.0	3.8381630858524156E-5	3.8381630858524156E-5
8	0.0	0.0	0.0	3.8381630858524156E-5	3.8381630858524156E-5
9	0.0	0.0	0.0	3.8381630858524156E-5	3.8381630858524156E-5
10-11	0.0	0.0	0.0	7.676326171704831E-5	7.676326171704831E-5
12-13	0.0	0.0	0.0	7.676326171704831E-5	1.1514489257557247E-4
14-15	0.0	3.8381630858524156E-5	0.0	7.676326171704831E-5	1.727173388633587E-4
16-17	0.0	7.676326171704831E-5	0.0	7.676326171704831E-5	1.9190815429262077E-4
18-19	0.0	7.676326171704831E-5	0.0	7.676326171704831E-5	1.9190815429262077E-4
20-21	0.0	7.676326171704831E-5	0.0	9.59540771463104E-5	1.9190815429262077E-4
22-23	0.0	7.676326171704831E-5	0.0	1.919081542926208E-4	1.9190815429262077E-4
24-25	0.0	9.59540771463104E-5	0.0	5.373428320193382E-4	2.878622314389312E-4
26-27	0.0	1.5352652343409662E-4	0.0	0.0014009295263361318	3.0705304686819325E-4
28-29	0.0	1.9190815429262077E-4	0.0	0.004548223256735113	3.0705304686819325E-4
30-31	0.0	1.9190815429262077E-4	0.0	0.015621323759419332	3.0705304686819325E-4
32-33	0.0	1.9190815429262077E-4	0.0	0.04488731728904401	3.454346777267174E-4
34-35	0.0	2.3028978515114495E-4	0.0	0.08664653166311828	3.454346777267174E-4
36-37	0.0	2.3028978515114495E-4	0.0	0.1474622257584498	3.454346777267174E-4
38-39	0.0	2.3028978515114495E-4	0.0	0.23499153493131414	3.6462549315597947E-4
40-41	0.0	2.3028978515114495E-4	0.0	0.33144457327878535	3.8381630858524155E-4
42-43	0.0	2.686714160096691E-4	0.0	0.4397959171923991	4.413887548730278E-4
44-45	0.0	2.686714160096691E-4	0.0	0.5813281809832069	4.98961201160814E-4
46-47	0.0	2.686714160096691E-4	0.0	0.7453712712725391	5.373428320193382E-4
48-49	0.0	2.878622314389312E-4	0.0	0.9053650995062971	5.373428320193382E-4
50-51	0.0	3.0705304686819325E-4	0.0	1.0659538430183622	5.373428320193382E-4
52-53	0.0	3.0705304686819325E-4	0.0	1.2384217012811405	5.757244628778623E-4
54-55	0.0	3.0705304686819325E-4	0.0	1.4606513439519953	6.141060937363865E-4
56-57	0.0	3.0705304686819325E-4	0.0	1.7134327647862353	6.141060937363865E-4
58-59	0.0	3.0705304686819325E-4	0.0	1.9665788111136315	6.141060937363865E-4
60-61	0.0	3.262438622974553E-4	0.0	2.2218550379536754	6.141060937363865E-4
62-63	0.0	3.454346777267174E-4	0.0	2.4967442781624256	6.141060937363865E-4
64-65	0.0	3.454346777267174E-4	0.0	2.8085566472570758	6.141060937363865E-4
66-67	0.0	3.454346777267174E-4	0.0	3.186116750012378	6.332969091656486E-4
68-69	0.0	3.454346777267174E-4	0.0	3.5851705660484536	6.524877245949106E-4
70-71	0.0	3.454346777267174E-4	0.0	3.99980732421309	7.676326171704831E-4
72-73	0.0	3.454346777267174E-4	0.0	4.423060758505466	8.827775097460556E-4
74-75	0.0	3.454346777267174E-4	0.0	4.894060941585845	8.827775097460556E-4
76-77	0.0	3.454346777267174E-4	0.0	5.43798622329742	8.827775097460556E-4
78-79	0.0	3.454346777267174E-4	0.0	6.037545678938425	8.827775097460556E-4
80-81	0.0	3.454346777267174E-4	0.0	6.634360847973047	8.827775097460556E-4
82-83	0.0	3.454346777267174E-4	0.0	7.247641736645975	9.211591406045798E-4
84-85	0.0	3.454346777267174E-4	0.0	7.910262211787536	9.211591406045798E-4
86-87	0.0	3.454346777267174E-4	0.0	8.64456038255739	9.211591406045798E-4
88-89	0.0	3.454346777267174E-4	0.0	9.442265007505528	9.211591406045798E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2800	0.0	57.206783	1
GTATCAA	4870	0.0	40.11331	1
TCAACGC	6345	0.0	29.843475	4
TATCAAC	6570	0.0	29.038143	2
CAACGCA	6550	0.0	29.035412	5
ATCAACG	6585	0.0	28.683718	3
AACGCAG	6900	0.0	27.491049	6
GTACATG	8885	0.0	27.336262	1
TACATGG	8890	0.0	26.211258	2
ACATGGG	9210	0.0	24.424566	3
ACCGTAT	195	1.5577825E-8	24.379488	8
TACCGTA	180	1.9371873E-7	23.77	7
ACGCAGA	8265	0.0	22.892826	7
CATGGGG	7505	0.0	22.19548	4
CGCAGAG	8480	0.0	21.919977	8
TACACCG	340	4.1836756E-11	19.577553	5
GTACCGT	275	2.8134309E-8	19.016367	6
ATGGGGG	5090	0.0	18.68192	5
GCAGAGT	9350	0.0	18.660086	9
AGAGTAC	8000	0.0	18.600025	10-11
>>END_MODULE