FastQCFastQC Report
Mon 15 Aug 2016
SRR2921379_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921379_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3681701
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33550.051.6295281
GTATCAA58450.040.839581
TCAACGC71700.032.4329574
CAACGCA73450.031.2682935
TATCAAC75500.030.9912512
ATCAACG76600.030.2343523
AACGCAG78300.029.3239236
GTACATG109150.026.7827261
TACATGG112500.025.4392262
ACGCAGA91200.024.3995297
ACATGGG117300.024.0729083
CGCAGAG92400.023.3678918
CATGGGG100550.021.1920934
ATGGGGG62500.019.8916995
GCAGAGT104050.019.2008369
AACGCGT1250.00607831818.9757296
GAGTACA83950.017.4676951
CAGAGTA95450.017.1026210-11
AGAGTAC88400.016.82927510-11
GTGTAGC19800.016.7776321
Produced by FastQC (version 0.11.5)