##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921379_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3681701
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.188027762167543	31.0	31.0	34.0	26.0	34.0
2	30.254838999690634	31.0	31.0	34.0	26.0	34.0
3	30.295478910427544	31.0	31.0	34.0	26.0	34.0
4	33.76608583912708	37.0	35.0	37.0	28.0	37.0
5	33.752227570897254	37.0	35.0	37.0	28.0	37.0
6	33.79081408294699	37.0	35.0	37.0	29.0	37.0
7	33.70564719948741	37.0	35.0	37.0	28.0	37.0
8	33.666178214906644	37.0	35.0	37.0	28.0	37.0
9	35.19180454903861	39.0	35.0	39.0	28.0	39.0
10-11	35.08247695834072	39.0	35.0	39.0	28.0	39.0
12-13	34.967234710260286	39.0	35.0	39.0	27.0	39.0
14-15	36.05702866691239	40.0	36.0	41.0	27.0	41.0
16-17	35.99574965484704	40.0	36.0	41.0	27.0	41.0
18-19	35.94900400657196	40.0	36.0	41.0	27.0	41.0
20-21	35.88726243657483	40.0	36.0	41.0	27.0	41.0
22-23	35.767709409319224	40.0	36.0	41.0	27.0	41.0
24-25	35.661788667792415	40.0	36.0	41.0	26.5	41.0
26-27	35.50847651669704	40.0	36.0	41.0	25.5	41.0
28-29	35.36533344234092	39.5	35.0	41.0	25.0	41.0
30-31	35.21929483138365	39.0	35.0	41.0	24.5	41.0
32-33	35.038425309388245	39.0	35.0	41.0	24.0	41.0
34-35	34.876192417580896	39.0	35.0	41.0	24.0	41.0
36-37	34.702127902292986	39.0	34.5	41.0	23.0	41.0
38-39	34.93837182867375	39.0	35.0	41.0	24.0	41.0
40-41	35.0660029155002	39.5	35.0	41.0	24.0	41.0
42-43	34.90722875105828	40.0	35.0	41.0	23.0	41.0
44-45	34.79147804235053	39.5	35.0	41.0	22.0	41.0
46-47	34.64626527249225	39.0	35.0	41.0	20.5	41.0
48-49	34.508413366539	39.0	35.0	41.0	19.5	41.0
50-51	32.80431721641709	37.0	32.0	39.5	15.5	40.0
52-53	32.962323665066776	37.5	32.5	39.5	14.5	40.0
54-55	33.82993024148349	38.5	34.0	40.0	14.5	41.0
56-57	33.85791390990197	38.5	34.0	41.0	14.0	41.0
58-59	33.684932589582914	38.0	34.0	40.5	13.0	41.0
60-61	33.45066682492685	38.0	33.0	40.0	11.0	41.0
62-63	33.164741379052785	37.0	33.0	40.0	8.0	41.0
64-65	32.88172124786885	37.0	33.0	40.0	7.0	41.0
66-67	32.54605412009286	36.0	33.0	39.5	4.0	41.0
68-69	32.17852210160467	36.0	32.0	39.0	2.0	41.0
70-71	31.789849990534265	35.0	32.0	39.0	2.0	40.5
72-73	31.430249224475318	35.0	32.0	37.5	2.0	40.0
74-75	31.026560956470934	35.0	31.5	37.0	2.0	39.0
76-77	30.63443704418148	35.0	31.0	36.5	2.0	39.0
78-79	30.283187852571405	35.0	31.0	36.0	2.0	38.5
80-81	29.926742014085338	35.0	31.0	36.0	2.0	37.0
82-83	29.622748153638767	35.0	30.0	35.0	2.0	37.0
84-85	29.3819532058687	35.0	30.0	35.0	2.0	36.5
86-87	29.14793094278976	34.5	30.0	35.0	2.0	36.0
88-89	28.939772811534667	34.0	30.0	35.0	2.0	36.0
90-91	28.683982213656133	34.0	30.0	35.0	2.0	35.5
92-93	28.48341608946517	34.0	29.5	35.0	2.0	35.0
94-95	28.2685530411079	34.0	29.0	35.0	2.0	35.0
96-97	27.999199011543848	34.0	29.0	35.0	2.0	35.0
98-99	27.7481014889585	34.0	29.0	35.0	2.0	35.0
100-101	26.68089926368274	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	134179.0
3	23328.0
4	16124.0
5	11982.0
6	10976.0
7	11315.0
8	12098.0
9	12782.0
10	13832.0
11	14804.0
12	14876.0
13	15235.0
14	16052.0
15	16919.0
16	16944.0
17	17803.0
18	16962.0
19	16005.0
20	16296.0
21	17285.0
22	18901.0
23	21723.0
24	25359.0
25	30481.0
26	35964.0
27	42322.0
28	49535.0
29	57910.0
30	69430.0
31	84545.0
32	104848.0
33	135406.0
34	187829.0
35	266720.0
36	430165.0
37	755415.0
38	800194.0
39	139143.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.457808684876674	18.43447210412395	13.434587598118943	24.673131612880432
2	16.22165089942575	19.83144769633408	38.925227676059976	25.021673728180193
3	19.14922526279976	23.70702082913824	29.43205142106034	27.71170248700166
4	13.767035578364476	16.130302539479278	36.21823476785305	33.8844271143032
5	14.634864138431977	36.929134264811104	34.049238048576086	14.386763548180836
6	33.32205043726203	34.812316358641795	17.327431455048075	14.538201749048108
7	28.87659031627307	31.760552169871342	21.019312797560353	18.343544716295238
8	26.51328380854581	34.3292352454574	20.262755756950927	18.894725189045865
9	26.850545834682617	15.76101721069023	19.73100553984713	37.65743141478002
10-11	25.613708081681132	25.339823009089972	27.759120774990297	21.287348134238595
12-13	26.06764437392684	22.931709013453887	27.451015018800668	23.549631593818603
14-15	23.474976947061325	24.63968192311303	24.7765118305358	27.108829299289845
16-17	23.601678843936416	27.423771160791038	25.81487452684705	23.159675468425498
18-19	23.581371435014034	26.909296148106993	27.004070388585255	22.505262028293718
20-21	24.090397521695692	26.09868796852809	26.88965609520362	22.921258414572605
22-23	24.100278244220412	25.877663574626418	26.408758273176613	23.613299907976558
24-25	23.847953612045284	26.05429979706153	26.521980789252265	23.575765801640923
26-27	23.548468824610204	26.280165870258486	26.586502834126346	23.584862471004968
28-29	23.6263064555039	26.56368515536198	26.346227823671704	23.463780565462415
30-31	24.14025035742256	26.10806426244364	26.25134568269135	23.50033969744245
32-33	23.82303989838447	26.156491938912247	26.148220465065997	23.87224769763729
34-35	24.13613680449597	26.29678967528409	26.205491422826626	23.361582097393313
36-37	24.079039646940526	26.198526419976282	26.2848329252731	23.437601007810095
38-39	23.87915817191667	26.31002728415684	26.283909790203047	23.52690475372344
40-41	24.040916999508767	26.048956818601294	26.327711653011416	23.582414528878527
42-43	23.89870747940995	26.06705460114491	26.105573111194502	23.928664808250637
44-45	23.817198887479794	26.112697099500892	26.25520473310945	23.814899279909866
46-47	23.959618304332935	26.07774267045171	26.019649790700978	23.942989234514382
48-49	23.712164808504088	26.248270623549836	26.159874601751838	23.87968996619424
50-51	23.812978176931843	26.094415811944003	26.328301950978894	23.76430406014526
52-53	24.0293037800706	25.98713485944147	26.285874473024574	23.69768688746336
54-55	23.52044730012684	25.84282796926717	26.561370749322695	24.075353981283296
56-57	23.60670052148616	26.02663420000909	26.288193846196563	24.07847143230819
58-59	23.708206356012994	26.013088831026593	26.41391369765532	23.864791115305092
60-61	23.83267183750761	25.89137260009573	26.54889441530397	23.72706114709269
62-63	23.722888001626576	25.869433444393845	26.63536177320846	23.772316780771117
64-65	23.517893392517976	25.709608740078053	26.569601565192112	24.202896302211858
66-67	23.572534906715386	25.938064819544877	26.309619292745573	24.179780980994167
68-69	23.565867231882898	25.837223821599814	26.46469603025953	24.13221291625775
70-71	23.485001342196774	25.65171374650318	26.51791706843	24.34536784287005
72-73	23.46091025267017	25.85044325044521	26.394328527825067	24.294317969059552
74-75	23.427076490345026	25.92383403643196	26.43862632072509	24.210463152497926
76-77	23.39217458839332	25.546704452498215	26.56172698738906	24.499393971719403
78-79	23.288063312610298	25.55629155386816	26.67194371559138	24.483701417930163
80-81	23.420960290473452	25.71805095535506	26.492418164826482	24.368570589345005
82-83	23.37401342398075	25.736011196949615	26.61200127780029	24.277974101269347
84-85	23.388138728427545	25.685008460826435	26.57224857095532	24.354604239790696
86-87	23.22810646634621	25.84439408360577	26.545895942552345	24.381603507495683
88-89	23.147511134330983	25.540286281420677	26.696884565322087	24.615318018926253
90-91	23.240420636983007	25.3830268270216	26.77578482844463	24.600767707550766
92-93	23.268203282643857	25.2887672136923	26.894409709597195	24.54861979406665
94-95	23.149710412659747	25.204765567063898	26.888852674304133	24.756671345972222
96-97	23.0805277864457	25.09847391972762	26.992922620982828	24.828075672843852
98-99	22.89091759534848	25.008372021602266	27.000232510778808	25.10047787227045
100-101	23.01961794983119	24.89500230151524	27.14640291751263	24.938976831140938
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	33.5
2	43.5
3	67.0
4	70.0
5	84.5
6	161.5
7	293.5
8	413.0
9	483.5
10	494.0
11	460.5
12	488.5
13	579.0
14	678.0
15	789.5
16	917.0
17	1033.5
18	1130.0
19	1246.0
20	1378.5
21	1500.5
22	1596.0
23	1761.0
24	2029.0
25	2372.5
26	3083.5
27	4358.0
28	6402.5
29	9680.0
30	14718.5
31	21897.0
32	31428.0
33	40061.5
34	48092.0
35	57441.0
36	69138.5
37	81025.5
38	91278.5
39	101243.5
40	109842.0
41	117436.5
42	125231.5
43	133802.5
44	140908.0
45	148695.5
46	154258.0
47	157370.0
48	161760.5
49	163863.0
50	162885.0
51	160539.5
52	156650.0
53	151232.5
54	145763.5
55	137721.0
56	126096.5
57	112657.0
58	100636.5
59	87989.0
60	72466.5
61	59124.0
62	48447.0
63	37267.5
64	26790.0
65	19687.0
66	15016.0
67	11660.5
68	9105.5
69	7008.5
70	5295.0
71	3854.5
72	2734.5
73	2024.0
74	1563.5
75	1136.0
76	815.0
77	588.0
78	412.5
79	282.0
80	204.0
81	160.0
82	121.5
83	99.0
84	79.0
85	70.5
86	66.5
87	56.5
88	42.5
89	33.0
90	31.5
91	26.5
92	19.5
93	18.0
94	22.0
95	19.0
96	10.5
97	7.5
98	7.0
99	8.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05041148099750632
2	0.05788085452892562
3	0.04910773579929494
4	0.05565362314864787
5	0.04731508615175431
6	0.01664991263549104
7	0.01398809952247616
8	0.019447532539986274
9	0.019909275631019466
10-11	0.02111795607519459
12-13	0.02664529248844488
14-15	0.04303717222012325
16-17	0.029144137451683337
18-19	0.023046412514215575
20-21	0.0260341619267833
22-23	0.04510143545062459
24-25	0.04132600664747083
26-27	0.030244172462674183
28-29	0.02034385736375659
30-31	0.013146097415297984
32-33	0.01015834800273026
34-35	0.009778088986585278
36-37	0.0014938747062838615
38-39	0.00707553383612629
40-41	0.004087784423558567
42-43	0.22116135992575173
44-45	0.1945024867581588
46-47	0.20133356836962044
48-49	0.19902485291445451
50-51	0.197286525983506
52-53	0.20183605349809774
54-55	0.20070885712881087
56-57	0.21126104482683414
58-59	0.20785229436067731
60-61	0.21268701613737778
62-63	0.2099029769120306
64-65	0.21117956075194588
66-67	0.20121134225728815
68-69	0.19977179026759642
70-71	0.18348855596910232
72-73	0.1909171874630775
74-75	0.19053692844693257
76-77	0.18890724694916833
78-79	0.2154031519669848
80-81	0.22408120594257924
82-83	0.22273671870692377
84-85	0.2174402538391901
86-87	0.23502723333589554
88-89	0.23364200406279598
90-91	0.23243332361862087
92-93	0.236751979587696
94-95	0.2484585250133023
96-97	0.24866223520052277
98-99	0.23774336916550257
100-101	0.24772516833930835
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3681701.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.898632059362285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85133569076021	42.39985904988979
2	12.996785305629826	14.530050395078565
3	4.484452729028744	7.520243192627425
4	2.1335186158308272	4.770430883925093
5	1.188953899483615	3.32304482813893
6	0.737517081575173	2.473571758827917
7	0.47587731590072796	1.8620623690852194
8	0.3423698568843393	1.5310405326555365
9	0.2691030092639523	1.3538241090821546
>10	1.4159432266254155	13.938556127832896
>50	0.07457179958694592	2.8283339189392005
>100	0.02781395501173155	2.6156089583635915
>500	0.0012693159741777032	0.5028659293920205
>1k	4.8819844425270517E-4	0.35050794616172704
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.7161358296070215E-5	0.0
6	0.0	0.0	0.0	2.7161358296070215E-5	2.7161358296070215E-5
7	0.0	0.0	0.0	2.7161358296070215E-5	2.7161358296070215E-5
8	0.0	0.0	0.0	5.432271659214043E-5	2.7161358296070215E-5
9	0.0	0.0	0.0	8.148407488821064E-5	2.7161358296070215E-5
10-11	0.0	0.0	0.0	8.148407488821064E-5	2.7161358296070215E-5
12-13	0.0	0.0	0.0	9.506475403624575E-5	4.074203744410532E-5
14-15	0.0	0.0	0.0	1.0864543318428086E-4	5.432271659214043E-5
16-17	0.0	0.0	0.0	2.037101872205266E-4	6.790339574017554E-5
18-19	0.0	0.0	0.0	2.851942621087373E-4	8.148407488821064E-5
20-21	0.0	0.0	0.0	2.9877494125677236E-4	1.2222611233231597E-4
22-23	0.0	0.0	0.0	4.210010535890883E-4	1.629681497764213E-4
24-25	0.0	0.0	0.0	5.024851284772989E-4	1.901295080724915E-4
26-27	0.0	0.0	0.0	7.876793905860363E-4	2.1729086636856172E-4
28-29	0.0	0.0	0.0	0.0030556528083078988	2.1729086636856172E-4
30-31	0.0	0.0	0.0	0.01082380128098398	2.3087154551659683E-4
32-33	0.0	0.0	0.0	0.031996080072770716	2.4445222466463193E-4
34-35	0.0	0.0	0.0	0.06348967501706412	2.7161358296070215E-4
36-37	0.0	1.3580679148035107E-5	0.0	0.11145663376792411	2.7161358296070215E-4
38-39	0.0	4.074203744410532E-5	0.0	0.18388239566439535	2.7161358296070215E-4
40-41	0.0	5.432271659214043E-5	0.0	0.2648096627075366	2.7161358296070215E-4
42-43	0.0	5.432271659214043E-5	0.0	0.35590885843255604	2.7161358296070215E-4
44-45	0.0	5.432271659214043E-5	0.0	0.4751608020314523	2.7161358296070215E-4
46-47	0.0	5.432271659214043E-5	0.0	0.6182468375351502	2.9877494125677236E-4
48-49	0.0	5.432271659214043E-5	0.0	0.7545289527856824	3.530976578489128E-4
50-51	0.0	5.432271659214043E-5	0.0	0.8882986423938283	3.80259016144983E-4
52-53	0.0	5.432271659214043E-5	0.0	1.0381750174715436	4.3458173273712344E-4
54-55	0.0	5.432271659214043E-5	0.0	1.2240809343289962	4.3458173273712344E-4
56-57	0.0	5.432271659214043E-5	0.0	1.4400816361784947	4.3458173273712344E-4
58-59	0.0	5.432271659214043E-5	0.0	1.6612429961042463	4.3458173273712344E-4
60-61	0.0	5.432271659214043E-5	0.0	1.888923082021055	4.617430910331936E-4
62-63	0.0	5.432271659214043E-5	0.0	2.130333234556527	4.617430910331936E-4
64-65	0.0	5.432271659214043E-5	0.0	2.410489064701343	4.889044493292639E-4
66-67	0.0	5.432271659214043E-5	0.0	2.7471676814602812	5.024851284772989E-4
68-69	0.0	5.432271659214043E-5	0.0	3.1066754198670665	5.16065807625334E-4
70-71	1.3580679148035107E-5	5.432271659214043E-5	0.0	3.4702437813391147	5.16065807625334E-4
72-73	2.7161358296070215E-5	5.432271659214043E-5	0.0	3.845844624536322	6.111305616615798E-4
74-75	2.7161358296070215E-5	5.432271659214043E-5	0.0	4.2611961155998275	6.790339574017553E-4
76-77	2.7161358296070215E-5	5.432271659214043E-5	0.0	4.75079317956564	6.790339574017553E-4
78-79	2.7161358296070215E-5	5.432271659214043E-5	0.0	5.282001444441034	6.790339574017553E-4
80-81	2.7161358296070215E-5	5.432271659214043E-5	0.0	5.814771487418451	6.790339574017553E-4
82-83	2.7161358296070215E-5	5.432271659214043E-5	0.0	6.361651856030678	7.333566739938957E-4
84-85	5.432271659214043E-5	5.432271659214043E-5	0.0	6.950795298151588	7.333566739938957E-4
86-87	8.148407488821064E-5	5.432271659214043E-5	0.0	7.612296598773231	7.469373531419309E-4
88-89	8.148407488821064E-5	5.432271659214043E-5	0.0	8.333824501229188	7.60518032289966E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3355	0.0	51.629528	1
GTATCAA	5845	0.0	40.83958	1
TCAACGC	7170	0.0	32.432957	4
CAACGCA	7345	0.0	31.268293	5
TATCAAC	7550	0.0	30.991251	2
ATCAACG	7660	0.0	30.234352	3
AACGCAG	7830	0.0	29.323923	6
GTACATG	10915	0.0	26.782726	1
TACATGG	11250	0.0	25.439226	2
ACGCAGA	9120	0.0	24.399529	7
ACATGGG	11730	0.0	24.072908	3
CGCAGAG	9240	0.0	23.367891	8
CATGGGG	10055	0.0	21.192093	4
ATGGGGG	6250	0.0	19.891699	5
GCAGAGT	10405	0.0	19.200836	9
AACGCGT	125	0.006078318	18.975729	6
GAGTACA	8395	0.0	17.467695	1
CAGAGTA	9545	0.0	17.10262	10-11
AGAGTAC	8840	0.0	16.829275	10-11
GTGTAGC	1980	0.0	16.777632	1
>>END_MODULE