FastQCFastQC Report
Mon 15 Aug 2016
SRR2921379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3681701
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32650.048.7645381
GTATCAA56150.041.4752351
TCAACGC70500.032.3128054
CAACGCA71700.031.6960325
ATCAACG73850.030.8470273
TATCAAC75350.030.4853062
AACGCAG76900.029.5503226
GTACATG111700.024.5933191
TACATGG117600.023.2053952
ACGCAGA95300.023.0956957
CGCAGAG96050.022.5194078
ACATGGG121850.021.655683
CATGGGG103150.019.9683914
GCAGAGT111200.018.7245889
ATGGGGG61800.017.386665
AGAGTAC94400.017.3484310-11
GAGTACA88250.016.6951181
ACCGCTC7700.016.6692478
CAGAGTA102950.016.1616110-11
AGTACAT90650.015.2353922
Produced by FastQC (version 0.11.5)