##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921379_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3681701
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50816130913401	33.0	31.0	34.0	30.0	34.0
2	31.64427529557669	34.0	31.0	34.0	30.0	34.0
3	31.717963245793182	34.0	31.0	34.0	29.0	34.0
4	35.21841100078469	37.0	35.0	37.0	33.0	37.0
5	34.972054493289924	37.0	35.0	37.0	32.0	37.0
6	34.92578321813749	37.0	35.0	37.0	32.0	37.0
7	34.79755526046249	37.0	35.0	37.0	32.0	37.0
8	34.75887069590931	37.0	35.0	37.0	32.0	37.0
9	36.384510040331904	39.0	37.0	39.0	32.0	39.0
10-11	36.24265332790468	39.0	37.0	39.0	32.0	39.0
12-13	36.18028636763279	39.0	37.0	39.0	32.0	39.0
14-15	37.428157935693314	40.0	38.0	41.0	32.0	41.0
16-17	37.40300393758211	40.0	38.0	41.0	32.0	41.0
18-19	37.3693810279542	40.0	38.0	41.0	32.0	41.0
20-21	37.33028659850433	40.0	38.0	41.0	31.0	41.0
22-23	37.22374834349666	40.0	38.0	41.0	31.0	41.0
24-25	37.153395129044966	40.0	37.5	41.0	31.0	41.0
26-27	37.02219599038597	40.0	37.0	41.0	31.0	41.0
28-29	36.87142274725731	40.0	37.0	41.0	30.0	41.0
30-31	36.739394915556694	40.0	37.0	41.0	30.0	41.0
32-33	36.699113127328914	40.0	37.0	41.0	30.0	41.0
34-35	36.83111855090894	40.0	37.0	41.0	30.0	41.0
36-37	36.81149392631286	40.0	37.0	41.0	30.0	41.0
38-39	36.72724591160444	40.0	37.0	41.0	30.0	41.0
40-41	36.6106200639324	40.0	37.0	41.0	30.0	41.0
42-43	36.51661107189313	40.0	37.0	41.0	30.0	41.0
44-45	36.39018336904599	40.0	36.0	41.0	30.0	41.0
46-47	36.246261171127145	40.0	36.0	41.0	29.0	41.0
48-49	36.099100931879036	40.0	36.0	41.0	28.5	41.0
50-51	35.954093637696275	40.0	35.5	41.0	28.0	41.0
52-53	35.78768332898299	39.0	35.0	41.0	28.0	41.0
54-55	35.58645568991072	39.0	35.0	41.0	28.0	41.0
56-57	35.32963323203052	39.0	35.0	41.0	27.0	41.0
58-59	35.109004370534166	39.0	35.0	41.0	26.5	41.0
60-61	34.843219479257	38.0	35.0	40.0	26.0	41.0
62-63	34.549206195723116	37.5	34.0	40.0	26.0	41.0
64-65	34.2396737268996	37.0	34.0	40.0	26.0	41.0
66-67	33.90611798731076	36.5	34.0	39.5	26.0	41.0
68-69	33.53809176790837	36.0	34.0	39.0	25.0	41.0
70-71	33.15383147626599	35.5	33.5	39.0	24.5	40.0
72-73	32.75384611623812	35.0	33.0	37.5	23.0	40.0
74-75	32.33918751142475	35.0	33.0	37.0	22.5	39.0
76-77	29.550756022827493	33.0	29.0	34.5	17.5	36.5
78-79	31.216376071821152	34.5	31.5	36.0	20.0	37.5
80-81	31.410903954449317	35.0	32.0	36.0	20.0	37.0
82-83	31.243814883392215	35.0	32.0	35.0	20.0	37.0
84-85	31.011653173356553	35.0	32.0	35.0	19.5	36.5
86-87	30.766570669372662	35.0	32.0	35.0	18.0	36.0
88-89	30.486717416759266	35.0	32.0	35.0	13.5	36.0
90-91	30.30433527872035	35.0	32.0	35.0	9.0	35.0
92-93	30.15934917039705	34.0	32.0	35.0	7.0	35.0
94-95	30.060915321477765	34.0	32.0	35.0	2.0	35.0
96-97	29.873471528513587	34.0	31.0	35.0	2.0	35.0
98-99	29.60767468623878	34.0	31.0	35.0	2.0	35.0
100-101	28.513405352580236	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57076.0
3	20116.0
4	11208.0
5	8333.0
6	7297.0
7	7375.0
8	7521.0
9	8225.0
10	9397.0
11	10422.0
12	10968.0
13	11735.0
14	12826.0
15	13968.0
16	15224.0
17	16025.0
18	14932.0
19	13869.0
20	13323.0
21	13301.0
22	14163.0
23	15804.0
24	18447.0
25	22582.0
26	27167.0
27	32817.0
28	39901.0
29	47837.0
30	58170.0
31	72647.0
32	91864.0
33	121513.0
34	175464.0
35	259731.0
36	444673.0
37	867117.0
38	961234.0
39	127427.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.39549036953856	19.846089981512733	14.05323222464149	25.70518742430722
2	15.234017657118148	21.048850418612332	37.710908691882636	26.006223232386883
3	17.822573131534224	24.7165340706449	29.245578480193984	28.215314317626888
4	13.375301198110467	17.2309086370485	35.30461515227159	34.08917501256944
5	14.437578941734513	37.270034865451635	34.0408305872681	14.251555605545763
6	33.334211562034774	35.31541402734044	17.101367239117863	14.249007171506928
7	28.650961038326095	31.893054693558266	21.12374870813579	18.332235559979846
8	26.479525276391996	34.802317556021954	19.997571732357102	18.720585435228948
9	26.549575861809526	15.773823023651296	19.70771119110433	37.968889923434844
10-11	25.540682791860895	25.580366556171153	27.762225869001707	21.11672478296624
12-13	25.983260683302774	23.046757176694463	27.491521220612864	23.478460919389903
14-15	23.363120096938722	24.787281057373068	24.74513792064704	27.104460925041174
16-17	23.486737230141426	27.68175925395273	25.77922626441013	23.05227725149571
18-19	23.534342362153055	27.098708963161506	26.951317492066146	22.415631182619293
20-21	24.071618469414474	26.173039126745536	26.861855850433336	22.893486553406657
22-23	23.99172446274399	25.960722705265198	26.41097733665843	23.63657549533238
24-25	23.73298312708467	26.234096761225977	26.424745493856	23.60817461783336
26-27	23.48494561036771	26.45453696017801	26.535208757969396	23.525308671484883
28-29	23.5642342011979	26.673878498961006	26.38895000611172	23.372937293729372
30-31	24.0691362384589	26.215456499519508	26.240584601906342	23.47482266011525
32-33	23.743572664209463	26.271110239403317	26.08323427014152	23.902082826245696
34-35	24.050543853174922	26.39001640326859	26.22144724991603	23.337992493640456
36-37	23.944166181946237	26.32983270796136	26.332983447346187	23.393017662746214
38-39	23.887176966044173	26.42573597215413	26.242152052247675	23.444935009554023
40-41	24.026994831889386	26.185845273941915	26.248930638558342	23.538229255610354
42-43	23.870136011911754	26.16891836555359	26.03627880710234	23.924666815432314
44-45	23.81223435623584	26.165083975143066	26.18322931003994	23.839452358581152
46-47	23.91256043292767	26.140870994748127	26.02541075919349	23.921157813130712
48-49	23.620583245003214	26.357304796773718	26.146371927483642	23.875740030739426
50-51	23.745650878950947	26.200390005027852	26.244855684829872	23.809103431191332
52-53	23.97606336204022	26.09152207177433	26.189644197627874	23.74277036855757
54-55	23.475091697667143	25.9644144604179	26.458633922052638	24.101859919862324
56-57	23.593397284873184	26.09096246610234	26.173035314141536	24.142604934882943
58-59	23.590849781325	26.116223102961623	26.314599987260202	23.978327128453174
60-61	23.73096522537499	26.046566270623835	26.415445670420652	23.807022833580522
62-63	23.633505503739023	26.009652698976893	26.49943236196915	23.857409435314935
64-65	23.379299870042395	25.796054864226498	26.45698289140592	24.36766237432518
66-67	23.433319023139042	26.10679390059668	26.16307915530002	24.296807920964252
68-69	23.50214958643826	26.010593614054688	26.233390423798898	24.253866375708153
70-71	23.379076865219844	25.859134775808418	26.265218104884685	24.496570254087054
72-73	23.372176408045046	26.01152944216132	26.157776790358376	24.458517359435255
74-75	23.328830251473008	26.04283700949179	26.13368651622434	24.49464622281086
76-77	23.23508538149493	25.70481885966411	26.34647851620493	24.713617242636033
78-79	23.19638312390861	25.70695198386882	26.36765947199121	24.729005420231353
80-81	23.289400272686088	25.929202168987654	26.151680209930785	24.629717348395474
82-83	23.209152162774664	25.913252119144815	26.23352130935195	24.644074408728578
84-85	23.15207574606549	25.866551573858825	26.21783460326672	24.763538076808963
86-87	23.024005286635084	26.02876741727842	26.13695750182027	24.810269794266222
88-89	22.95057547186896	25.747646391064883	26.239779786542943	25.06199835052321
90-91	22.930308328522802	25.5927510841234	26.357989814710237	25.11895077264356
92-93	22.941934112813623	25.51083841212992	26.438385443396506	25.10884203165995
94-95	22.72183402258575	25.47933261352293	26.440578292962837	25.358255070928486
96-97	22.636619657095203	25.376136558877636	26.505412088117307	25.481831695909847
98-99	22.439332179320832	25.27155645285928	26.421218547025283	25.86789282079461
100-101	22.4251382666595	25.304238258130717	26.438728354158215	25.831895121051573
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	29.0
2	35.0
3	54.0
4	53.0
5	58.0
6	90.5
7	199.5
8	302.5
9	323.0
10	299.5
11	268.0
12	275.5
13	308.5
14	344.0
15	388.0
16	419.0
17	453.0
18	525.0
19	599.0
20	691.5
21	802.0
22	951.5
23	1155.5
24	1421.0
25	1872.0
26	2708.0
27	4051.5
28	6059.5
29	9556.0
30	15076.0
31	22021.0
32	31663.0
33	40768.0
34	48656.5
35	57928.0
36	69297.0
37	80550.0
38	90961.0
39	101398.5
40	109821.0
41	117492.5
42	124920.0
43	133312.5
44	141275.0
45	149546.5
46	154860.5
47	158335.5
48	163469.0
49	165537.5
50	164356.5
51	161320.5
52	157745.5
53	153805.0
54	148295.0
55	139517.0
56	127715.5
57	113983.5
58	101315.0
59	88086.5
60	72033.0
61	58142.0
62	47345.0
63	36089.0
64	25708.5
65	18826.5
66	14178.5
67	10884.5
68	8495.5
69	6496.0
70	4779.5
71	3382.5
72	2376.0
73	1739.5
74	1288.0
75	943.0
76	669.5
77	412.0
78	219.0
79	126.5
80	73.5
81	45.0
82	33.5
83	20.5
84	11.0
85	8.5
86	7.0
87	5.0
88	3.5
89	4.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1536518038808692
2	3.530976578489128E-4
3	0.0059211761085433065
4	0.004182849177594813
5	0.02661813113014881
6	0.0013037451982113703
7	1.629681497764213E-4
8	0.0017383269309484935
9	0.0
10-11	0.10523668271812404
12-13	2.58032903812667E-4
14-15	0.005432271659214043
16-17	1.0864543318428085E-4
18-19	2.4445222466463193E-4
20-21	6.790339574017554E-4
22-23	1.765488289244564E-4
24-25	0.001656842856060283
26-27	0.0031778789206402145
28-29	0.006817500932313624
30-31	0.015305425399835565
32-33	0.014911585704542547
34-35	0.011475673880089665
36-37	6.926146365497905E-4
38-39	1.629681497764213E-4
40-41	0.004929786530736744
42-43	0.008026181376488747
44-45	0.008528666504966047
46-47	0.009424991328736363
48-49	0.011815190858790542
50-51	0.005703885242174744
52-53	0.0017519076100965289
54-55	0.0010049702569545978
56-57	0.005853272712803131
58-59	0.008813860767074784
60-61	0.00763234168119573
62-63	0.0178721737588142
64-65	0.03449492503600917
66-67	0.03642338147503016
68-69	0.02697122878799772
70-71	0.041964298567428476
72-73	0.025816871060414737
74-75	0.024132866846058382
76-77	0.24547077560073458
78-79	0.07101337126507558
80-81	0.054648652891693274
82-83	0.019963598347611605
84-85	0.03240350044721176
86-87	0.08122604198439798
88-89	0.261495976995416
90-91	0.32051760857277656
92-93	0.05273377713182032
94-95	0.004780399060108357
96-97	0.009737346949141171
98-99	0.0014667133479877915
100-101	0.0012765838399153
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3681701.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.361035622505916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5623977880635	40.53289162256376
2	13.594440257856055	14.78015702250683
3	4.8089847162125245	7.842641683983466
4	2.1961189410083795	4.775331999336659
5	1.2431564316063977	3.378963553145136
6	0.7957907869121204	2.5956006789235073
7	0.5034907619926324	1.9159195473798896
8	0.3746558093598742	1.6293342239032722
9	0.3014384102431555	1.4747853741497754
>10	1.5072002910592255	14.479766450240572
>50	0.08051131100011767	2.980641033252846
>100	0.02986196782350163	2.72164438144511
>500	0.0015019437501085145	0.5761844111322676
>1k	4.505831123365165E-4	0.31613801803686575
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7161358296070215E-5
2	0.0	0.0	0.0	0.0	2.7161358296070215E-5
3	0.0	0.0	0.0	0.0	2.7161358296070215E-5
4	0.0	0.0	0.0	0.0	2.7161358296070215E-5
5	0.0	0.0	0.0	0.0	2.7161358296070215E-5
6	0.0	0.0	0.0	0.0	2.7161358296070215E-5
7	0.0	0.0	0.0	0.0	2.7161358296070215E-5
8	0.0	0.0	0.0	2.7161358296070215E-5	2.7161358296070215E-5
9	0.0	0.0	0.0	5.432271659214043E-5	2.7161358296070215E-5
10-11	0.0	0.0	0.0	1.0864543318428086E-4	4.074203744410532E-5
12-13	0.0	0.0	0.0	1.4938747062838618E-4	8.148407488821064E-5
14-15	0.0	0.0	0.0	1.629681497764213E-4	1.629681497764213E-4
16-17	0.0	0.0	0.0	1.901295080724915E-4	1.629681497764213E-4
18-19	0.0	0.0	0.0	2.1729086636856172E-4	1.629681497764213E-4
20-21	0.0	0.0	0.0	2.1729086636856172E-4	1.901295080724915E-4
22-23	0.0	0.0	0.0	3.395169787008777E-4	2.037101872205266E-4
24-25	0.0	0.0	0.0	4.210010535890883E-4	2.1729086636856172E-4
26-27	0.0	0.0	0.0	6.926146365497905E-4	2.1729086636856172E-4
28-29	0.0	0.0	0.0	0.003028491450011829	2.1729086636856172E-4
30-31	0.0	0.0	0.0	0.010837381960132016	2.1729086636856172E-4
32-33	0.0	0.0	0.0	0.03286524353824496	2.1729086636856172E-4
34-35	0.0	0.0	0.0	0.06530948602290082	2.1729086636856172E-4
36-37	0.0	0.0	0.0	0.11381967193968223	2.1729086636856172E-4
38-39	0.0	0.0	0.0	0.18696520983099932	2.1729086636856172E-4
40-41	0.0	1.3580679148035107E-5	0.0	0.26911473799746366	2.1729086636856172E-4
42-43	0.0	2.7161358296070215E-5	0.0	0.3614633562041024	2.3087154551659683E-4
44-45	0.0	2.7161358296070215E-5	0.0	0.4825079494505393	2.4445222466463193E-4
46-47	0.0	2.7161358296070215E-5	0.0	0.6276446675055904	2.4445222466463193E-4
48-49	0.0	2.7161358296070215E-5	0.0	0.7652984313500744	2.4445222466463193E-4
50-51	0.0	2.7161358296070215E-5	0.0	0.9004126081938756	2.58032903812667E-4
52-53	0.0	2.7161358296070215E-5	0.0	1.0511853080953615	2.851942621087373E-4
54-55	0.0	2.7161358296070215E-5	0.0	1.2383406474344332	2.9877494125677236E-4
56-57	0.0	2.7161358296070215E-5	0.0	1.4557673205944752	2.9877494125677236E-4
58-59	0.0	2.7161358296070215E-5	0.0	1.6782052643601422	2.9877494125677236E-4
60-61	0.0	5.432271659214043E-5	0.0	1.9073656443040865	2.9877494125677236E-4
62-63	0.0	5.432271659214043E-5	0.0	2.1502017681501027	2.9877494125677236E-4
64-65	0.0	5.432271659214043E-5	0.0	2.432856443258157	2.9877494125677236E-4
66-67	0.0	5.432271659214043E-5	0.0	2.7725635514671074	3.259362995528426E-4
68-69	0.0	5.432271659214043E-5	0.0	3.134950393853276	3.259362995528426E-4
70-71	0.0	5.432271659214043E-5	0.0	3.503204089631396	3.395169787008777E-4
72-73	0.0	5.432271659214043E-5	0.0	3.881928489032651	3.9383969529301814E-4
74-75	2.7161358296070215E-5	5.432271659214043E-5	0.0	4.303432027750216	4.074203744410532E-4
76-77	2.7161358296070215E-5	5.432271659214043E-5	0.0	4.801829371803957	4.210010535890883E-4
78-79	2.7161358296070215E-5	5.432271659214043E-5	0.0	5.342612015478715	4.3458173273712344E-4
80-81	2.7161358296070215E-5	5.432271659214043E-5	0.0	5.883937886319394	4.7532377018122873E-4
82-83	2.7161358296070215E-5	5.432271659214043E-5	0.0	6.441574152816864	5.703885242174745E-4
84-85	2.7161358296070215E-5	5.432271659214043E-5	0.0	7.040332715774584	5.703885242174745E-4
86-87	2.7161358296070215E-5	8.148407488821064E-5	0.0	7.715808535239554	5.839692033655096E-4
88-89	2.7161358296070215E-5	8.148407488821064E-5	0.0	8.452750508528531	5.975498825135447E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3265	0.0	48.764538	1
GTATCAA	5615	0.0	41.475235	1
TCAACGC	7050	0.0	32.312805	4
CAACGCA	7170	0.0	31.696032	5
ATCAACG	7385	0.0	30.847027	3
TATCAAC	7535	0.0	30.485306	2
AACGCAG	7690	0.0	29.550322	6
GTACATG	11170	0.0	24.593319	1
TACATGG	11760	0.0	23.205395	2
ACGCAGA	9530	0.0	23.095695	7
CGCAGAG	9605	0.0	22.519407	8
ACATGGG	12185	0.0	21.65568	3
CATGGGG	10315	0.0	19.968391	4
GCAGAGT	11120	0.0	18.724588	9
ATGGGGG	6180	0.0	17.38666	5
AGAGTAC	9440	0.0	17.34843	10-11
GAGTACA	8825	0.0	16.695118	1
ACCGCTC	770	0.0	16.669247	8
CAGAGTA	10295	0.0	16.16161	10-11
AGTACAT	9065	0.0	15.235392	2
>>END_MODULE