FastQCFastQC Report
Mon 15 Aug 2016
SRR2921378_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921378_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528763
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26050.052.832961
GTATCAA43600.040.2744751
TCAACGC54500.031.8725224
CAACGCA56700.030.5503255
ATCAACG57200.030.3674493
TATCAAC57850.030.1915132
AACGCAG58950.029.535366
GTACATG70150.024.89631
ACGCAGA69450.024.8011447
CGCAGAG72500.023.4978528
TACATGG73450.023.3268362
ACATGGG76600.021.561243
GTGGTAT15000.020.2489911
TATACCG1656.370132E-520.1335415
GCAGAGT78500.019.9491589
CATGGGG64550.019.5576744
ATACCGT2552.712768E-718.6045786
AGAGTAC66200.018.02852810-11
GTCTTAG7800.017.6448541
CAGAGTA73550.017.58183110-11
Produced by FastQC (version 0.11.5)