FastQCFastQC Report
Mon 15 Aug 2016
SRR2921378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528763
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26100.053.9020461
GTATCAA43400.037.7818641
TCAACGC55100.029.2850044
CAACGCA55950.028.8071235
ATCAACG56550.028.6180293
TATCAAC58400.027.63022
AACGCAG59900.026.9048236
GTACATG70300.022.8515381
ACGCAGA71850.022.0330567
TACATGG73200.021.7844262
GTGGTAT14600.020.8344061
ACATGGG75250.020.560283
CGCAGAG76600.020.4185288
CATGGGG63550.018.296524
TGGTATC16500.017.8330542
GCAGAGT84850.017.760899
GTATAGG7000.017.653441
GAGTACT34900.017.40304212-13
AGAGTAC70950.017.1866910-11
CAGAGTA77800.016.89556910-11
Produced by FastQC (version 0.11.5)