##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921378_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2528763
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.453929450881716	33.0	31.0	34.0	29.0	34.0
2	31.58705857369789	34.0	31.0	34.0	29.0	34.0
3	31.653952149726962	34.0	31.0	34.0	28.0	34.0
4	35.160180293685094	37.0	35.0	37.0	33.0	37.0
5	34.90177015402392	37.0	35.0	37.0	32.0	37.0
6	34.85989948445149	37.0	35.0	37.0	32.0	37.0
7	34.730414435832856	37.0	35.0	37.0	32.0	37.0
8	34.68890995320637	37.0	35.0	37.0	32.0	37.0
9	36.30846742063214	39.0	37.0	39.0	32.0	39.0
10-11	36.16577057636481	39.0	37.0	39.0	32.0	39.0
12-13	36.104455221782345	39.0	37.0	39.0	32.0	39.0
14-15	37.33796267186763	40.0	38.0	41.0	31.5	41.0
16-17	37.311425586343994	40.0	38.0	41.0	31.5	41.0
18-19	37.282024254546585	40.0	38.0	41.0	31.0	41.0
20-21	37.245675454757915	40.0	38.0	41.0	31.0	41.0
22-23	37.13371992551299	40.0	37.5	41.0	31.0	41.0
24-25	37.063107732911305	40.0	37.0	41.0	31.0	41.0
26-27	36.92182561196917	40.0	37.0	41.0	30.0	41.0
28-29	36.765605950419236	40.0	37.0	41.0	30.0	41.0
30-31	36.62568477156617	40.0	37.0	41.0	30.0	41.0
32-33	36.573743763254996	40.0	36.5	41.0	30.0	41.0
34-35	36.69921538712802	40.0	37.0	41.0	30.0	41.0
36-37	36.667819008740636	40.0	37.0	41.0	30.0	41.0
38-39	36.574500852788496	40.0	37.0	41.0	30.0	41.0
40-41	36.4485254252771	40.0	37.0	41.0	30.0	41.0
42-43	36.33812302695033	40.0	36.0	41.0	29.5	41.0
44-45	36.19692830051689	40.0	36.0	41.0	29.0	41.0
46-47	36.032542393257096	40.0	36.0	41.0	28.0	41.0
48-49	35.86420455376799	40.0	35.5	41.0	28.0	41.0
50-51	35.711775085288735	39.5	35.0	41.0	27.5	41.0
52-53	35.53926425687184	39.0	35.0	41.0	27.0	41.0
54-55	35.32876687139127	39.0	35.0	41.0	26.5	41.0
56-57	35.05815195018276	39.0	35.0	41.0	26.0	41.0
58-59	34.82868540863655	38.5	35.0	40.5	26.0	41.0
60-61	34.555531301272595	38.0	34.0	40.0	26.0	41.0
62-63	34.26420704510466	37.5	34.0	40.0	25.0	41.0
64-65	33.94727659333832	37.0	34.0	40.0	24.5	41.0
66-67	33.60565620423899	36.5	34.0	39.0	23.5	41.0
68-69	33.23885551947731	36.0	33.5	39.0	23.0	41.0
70-71	32.85858283279216	35.0	33.0	38.5	22.0	40.0
72-73	32.46341393005196	35.0	33.0	37.5	21.5	40.0
74-75	32.058772411649485	35.0	33.0	37.0	20.0	39.0
76-77	29.2913499604352	33.0	28.5	34.5	13.0	36.5
78-79	30.95387468101993	34.5	31.5	36.0	17.5	37.5
80-81	31.155211065647514	35.0	32.0	36.0	18.0	37.0
82-83	30.995533586975135	35.0	32.0	35.0	18.0	37.0
84-85	30.768189031554165	35.0	32.0	35.0	16.0	36.5
86-87	30.523975754153316	35.0	32.0	35.0	11.0	36.0
88-89	30.2400128046796	35.0	31.5	35.0	7.0	36.0
90-91	30.057966286283055	34.0	31.0	35.0	2.0	35.0
92-93	29.919888498843108	34.0	31.0	35.0	2.0	35.0
94-95	29.817183935386588	34.0	31.0	35.0	2.0	35.0
96-97	29.624977508766143	34.0	31.0	35.0	2.0	35.0
98-99	29.352773866115566	34.0	31.0	35.0	2.0	35.0
100-101	28.256083112573222	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41231.0
3	14566.0
4	8093.0
5	5883.0
6	5189.0
7	5553.0
8	5799.0
9	6167.0
10	6973.0
11	7578.0
12	7922.0
13	8649.0
14	9641.0
15	10963.0
16	11598.0
17	12327.0
18	11601.0
19	9882.0
20	9703.0
21	9440.0
22	9912.0
23	11259.0
24	13290.0
25	16202.0
26	19285.0
27	23182.0
28	27982.0
29	33642.0
30	41203.0
31	50762.0
32	64423.0
33	85480.0
34	120485.0
35	179341.0
36	311263.0
37	597253.0
38	632605.0
39	82428.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.101711616162014	20.13630196175656	14.397211544478337	25.364774877603086
2	15.379860816168673	21.262034640149132	37.15816006536056	26.19994447832163
3	17.81746565320214	24.6059510721263	29.480625795888667	28.095957478782896
4	13.613063402951454	17.49497360649073	34.89788234996354	33.99408064059428
5	14.61996446388084	36.93561095702281	33.93588625910356	14.508538319992786
6	33.35536012217947	35.26393985302556	16.894388863382105	14.486311161412862
7	28.553497719437416	31.913600410002996	21.045396917543048	18.487504953016533
8	26.498969241762932	34.456509832440986	19.96867190963337	19.075849016162717
9	26.3786286022059	15.842330815501493	19.85002153226696	37.929019050025644
10-11	25.451207303396806	25.5979909483419	27.70559111887694	21.24521062938436
12-13	25.85208792138753	23.187599134595267	27.483421710016653	23.47689123400055
14-15	23.41695885572096	24.926916484682913	24.800622000827328	26.8555026587688
16-17	23.419196199245402	27.813067348001653	25.741637900932513	23.026098551820436
18-19	23.373301586510685	27.126667370569805	27.142307465683736	22.35772357723577
20-21	23.886469487364792	26.189566178513275	27.095271473328197	22.828692860793733
22-23	23.903073385615905	25.91210271759838	26.6936126957161	23.491211201069614
24-25	23.723426299925418	26.256543819368044	26.583013079334144	23.437016801372394
26-27	23.353080170444997	26.479084896240124	26.74592424886551	23.421910684449365
28-29	23.534920564927	26.557038362919506	26.671589071233615	23.236452000919886
30-31	23.942304687917144	26.18592117945649	26.45888565313914	23.412888479487222
32-33	23.655555039190464	26.274389598781028	26.325015350875024	23.74504001115349
34-35	23.986875100480468	26.35650360998016	26.348297087198556	23.308324202340817
36-37	23.956269972867943	26.210393859716074	26.455713372216948	23.377622795199038
38-39	23.73527415319646	26.299179498880775	26.488402712871668	23.4771436350511
40-41	23.92151899985664	26.142339501015865	26.47103430206979	23.465107197057705
42-43	23.857246983513285	26.145125945167152	26.168499292669512	23.82912777865005
44-45	23.746007815989856	26.16578891844268	26.27375659695369	23.814446668613776
46-47	23.882034059986722	26.15523097698466	26.14482959229538	23.817905370733232
48-49	23.646043298059425	26.301483853450957	26.212773396196603	23.839699452293015
50-51	23.689302803095945	26.29151659991687	26.24550294095896	23.773677656028227
52-53	23.99797841827048	26.16888512061274	26.130683869620636	23.702452591496147
54-55	23.574883143383662	26.061793620538293	26.336297919121776	24.027025316956273
56-57	23.59424409674271	26.198922409361053	26.127855528188675	24.07897796570756
58-59	23.595393414423235	26.127457252006664	26.322611671970513	23.954537661599588
60-61	23.621298630009132	26.092372052518492	26.48886301554602	23.797466301926356
62-63	23.59660820325625	26.117871205938116	26.417541752256923	23.867978838548712
64-65	23.44378552629653	25.941153875518662	26.34825373504528	24.26680686313952
66-67	23.42979450293573	26.08397664076031	26.2385167942996	24.247712062004364
68-69	23.36782530117265	26.040133674752326	26.32829742296169	24.263743601113337
70-71	23.377265095493623	25.86756460793731	26.28031006746032	24.474860229108742
72-73	23.27773737619861	26.032859174598705	26.222745497937872	24.466657951264818
74-75	23.232514215080347	26.05157972805933	26.20695179233622	24.508954264524103
76-77	23.223841347843297	25.862256570420932	26.27143687593562	24.642465205800153
78-79	23.118092344391385	25.86791753215378	26.29193861330267	24.72205151015217
80-81	23.239962546438598	25.913781236936906	26.181744732177954	24.664511484446543
82-83	23.161815092705996	25.872758242955708	26.338972907774178	24.626453756564114
84-85	23.097382135163123	25.862896953960107	26.238677530857164	24.801043380019607
86-87	23.023059629234073	26.028713076423944	26.138402102198192	24.80982519214379
88-89	22.816163937429152	25.80419034206364	26.234758436609013	25.144887283898193
90-91	22.757549448807872	25.635943237039093	26.374191787527867	25.232315526625165
92-93	22.79594652739655	25.52831149819236	26.461460852534902	25.21428112187619
94-95	22.64318644020877	25.455296842883712	26.490334278830325	25.411182438077194
96-97	22.61502706420286	25.352750190872186	26.523828422465357	25.508394322459598
98-99	22.39087953421687	25.332671868354495	26.434390695252024	25.842057902176606
100-101	22.29171910569355	25.30975523093505	26.464504620005968	25.934021043365433
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	83.0
1	63.5
2	45.5
3	56.5
4	65.5
5	75.0
6	115.5
7	255.0
8	363.0
9	398.5
10	369.0
11	303.0
12	325.0
13	353.5
14	381.5
15	396.0
16	450.0
17	529.0
18	606.0
19	682.5
20	774.5
21	903.5
22	1043.5
23	1299.0
24	1612.5
25	2069.5
26	2846.0
27	4057.0
28	5918.5
29	8880.0
30	12940.5
31	17891.5
32	24515.5
33	30272.5
34	34876.0
35	40513.5
36	47764.5
37	55185.5
38	62073.0
39	68481.0
40	73064.0
41	77683.5
42	83255.5
43	88898.5
44	93946.0
45	99215.0
46	102761.0
47	105346.0
48	108719.0
49	110087.5
50	109083.5
51	107489.5
52	106088.0
53	104748.5
54	102351.5
55	96580.5
56	88836.5
57	80196.5
58	71807.5
59	62798.0
60	51695.0
61	42261.0
62	34620.0
63	26490.5
64	18967.5
65	13534.0
66	10073.0
67	7687.5
68	5726.0
69	4349.0
70	3250.0
71	2292.5
72	1612.0
73	1124.0
74	811.0
75	570.0
76	387.5
77	234.5
78	116.5
79	73.5
80	47.5
81	29.5
82	16.0
83	11.0
84	8.0
85	7.5
86	6.5
87	3.5
88	3.5
89	3.0
90	1.5
91	1.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.149519745424937
2	8.304455577687589E-4
3	0.005654938798139644
4	0.004389497948206297
5	0.02495291175962318
6	9.490806374500102E-4
7	3.954502656041709E-5
8	0.0016608911155375178
9	0.0
10-11	0.10265888895084276
12-13	1.5818010624166836E-4
14-15	0.0058922089575021465
16-17	7.909005312083418E-5
18-19	2.3727015936250256E-4
20-21	6.129479116864649E-4
22-23	1.779526195218769E-4
24-25	0.0016213460889771007
26-27	0.003479962337316704
28-29	0.0074542375066386215
30-31	0.01629255094289184
32-33	0.01603550827024913
34-35	0.011329650109559495
36-37	7.909005312083418E-4
38-39	1.384075929614598E-4
40-41	0.004962900833332345
42-43	0.009569896427620935
44-45	0.009036038569055304
46-47	0.010024664233065732
48-49	0.01235782080013034
50-51	0.0065644744090292365
52-53	0.0019772513280208546
54-55	9.09535610889593E-4
56-57	0.0062876592231063165
58-59	0.009985119206505315
60-61	0.00814627547144592
62-63	0.019238655421642912
64-65	0.03487871342628787
66-67	0.037211869993352485
68-69	0.02714766073372633
70-71	0.04172000302124003
72-73	0.026554485335320076
74-75	0.025229726945546104
76-77	0.23871355283195778
78-79	0.06983651690569657
80-81	0.05403827879480995
82-83	0.02089954653718043
84-85	0.032624646912344096
86-87	0.08203615759958525
88-89	0.26218352609556533
90-91	0.3163404399700565
92-93	0.05142830704182243
94-95	0.004369725434926089
96-97	0.009431488834659477
98-99	0.0012852133632135555
100-101	0.0012852133632135555
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2528763.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.72678776355668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.59351061762013	47.72731438869055
2	12.010429062049104	14.587095532006177
3	3.8036434860198463	6.929491521112861
4	1.7577594823488196	4.2697234809590405
5	0.9845738730939047	2.989500431445827
6	0.6058523987183042	2.2074882019804902
7	0.42738597868544825	1.816764433452582
8	0.31741235775911675	1.5420346306534403
9	0.23151627104348324	1.2653315509920902
>10	1.2059679657522484	12.729447896439241
>50	0.04786661110764219	1.9399906672366223
>100	0.012840968806992786	1.404697089877468
>500	9.143672594412757E-4	0.3633421849587879
>1k	3.2655973550621054E-4	0.22777799019489883
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.954502656041709E-5
2	0.0	0.0	0.0	0.0	3.954502656041709E-5
3	0.0	0.0	0.0	0.0	3.954502656041709E-5
4	0.0	0.0	0.0	0.0	3.954502656041709E-5
5	0.0	0.0	0.0	0.0	7.909005312083418E-5
6	0.0	0.0	0.0	0.0	1.5818010624166836E-4
7	0.0	0.0	0.0	0.0	1.9772513280208546E-4
8	0.0	0.0	0.0	0.0	1.9772513280208546E-4
9	0.0	0.0	0.0	0.0	1.9772513280208546E-4
10-11	0.0	0.0	0.0	0.0	2.3727015936250253E-4
12-13	0.0	0.0	0.0	0.0	2.3727015936250253E-4
14-15	0.0	0.0	0.0	0.0	2.768151859229196E-4
16-17	0.0	0.0	0.0	0.0	2.768151859229196E-4
18-19	0.0	0.0	0.0	0.0	2.768151859229196E-4
20-21	0.0	1.9772513280208544E-5	0.0	0.0	2.768151859229196E-4
22-23	0.0	3.954502656041709E-5	0.0	1.9772513280208544E-5	2.768151859229196E-4
24-25	0.0	3.954502656041709E-5	0.0	2.3727015936250253E-4	3.163602124833367E-4
26-27	0.0	3.954502656041709E-5	0.0	0.0012456683366531384	3.163602124833367E-4
28-29	0.0	3.954502656041709E-5	0.0	0.0038951851162010833	3.163602124833367E-4
30-31	0.0	3.954502656041709E-5	0.0	0.012674181012613677	3.163602124833367E-4
32-33	0.0	3.954502656041709E-5	0.0	0.03549166133797434	3.163602124833367E-4
34-35	0.0	3.954502656041709E-5	0.0	0.07141831796811327	3.559052390437538E-4
36-37	0.0	3.954502656041709E-5	0.0	0.12670226509957636	3.559052390437538E-4
38-39	0.0	3.954502656041709E-5	0.0	0.2024705359893355	3.756777523239624E-4
40-41	0.0	3.954502656041709E-5	0.0	0.28177808675625193	4.3499529216458797E-4
42-43	0.0	3.954502656041709E-5	0.0	0.3718023397210415	4.3499529216458797E-4
44-45	0.0	3.954502656041709E-5	0.0	0.49729848151052514	4.3499529216458797E-4
46-47	0.0	3.954502656041709E-5	0.0	0.6431603119786236	4.3499529216458797E-4
48-49	0.0	3.954502656041709E-5	0.0	0.786649440853097	4.7454031872500507E-4
50-51	0.0	3.954502656041709E-5	0.0	0.9261445220449682	5.140853452854222E-4
52-53	0.0	3.954502656041709E-5	0.0	1.0767715282135968	5.734028851260477E-4
54-55	0.0	3.954502656041709E-5	0.0	1.2650453996677427	6.52492938246882E-4
56-57	0.0	3.954502656041709E-5	0.0	1.4920733971510973	6.722654515270905E-4
58-59	0.0	3.954502656041709E-5	0.0	1.724815650972432	6.920379648072991E-4
60-61	0.0	3.954502656041709E-5	0.0	1.9535243120846042	7.118104780875076E-4
62-63	0.0	3.954502656041709E-5	0.0	2.1906560638541452	7.118104780875076E-4
64-65	0.0	3.954502656041709E-5	0.0	2.4727505108228804	7.118104780875076E-4
66-67	0.0	3.954502656041709E-5	0.0	2.8189079008194913	7.315829913677162E-4
68-69	0.0	3.954502656041709E-5	0.0	3.1867755103977715	7.711280179281332E-4
70-71	0.0	3.954502656041709E-5	0.0	3.555038570241656	9.490806374500101E-4
72-73	0.0	3.954502656041709E-5	0.0	3.9316060856632276	0.0011863507968125128
74-75	0.0	3.954502656041709E-5	0.0	4.349458608813874	0.0011863507968125128
76-77	0.0	3.954502656041709E-5	0.0	4.851502493511649	0.0012061233100927214
78-79	0.0	3.954502656041709E-5	0.0	5.403511519268513	0.0012258958233729298
80-81	0.0	3.954502656041709E-5	0.0	5.961966384354723	0.0012654408499333468
82-83	0.0	3.954502656041709E-5	0.0	6.517534462502022	0.001304985876493764
84-85	0.0	3.954502656041709E-5	0.0	7.116087984520495	0.001304985876493764
86-87	0.0	3.954502656041709E-5	0.0	7.7909436352872925	0.001304985876493764
88-89	0.0	3.954502656041709E-5	0.0	8.53794918701357	0.001304985876493764
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2610	0.0	53.902046	1
GTATCAA	4340	0.0	37.781864	1
TCAACGC	5510	0.0	29.285004	4
CAACGCA	5595	0.0	28.807123	5
ATCAACG	5655	0.0	28.618029	3
TATCAAC	5840	0.0	27.6302	2
AACGCAG	5990	0.0	26.904823	6
GTACATG	7030	0.0	22.851538	1
ACGCAGA	7185	0.0	22.033056	7
TACATGG	7320	0.0	21.784426	2
GTGGTAT	1460	0.0	20.834406	1
ACATGGG	7525	0.0	20.56028	3
CGCAGAG	7660	0.0	20.418528	8
CATGGGG	6355	0.0	18.29652	4
TGGTATC	1650	0.0	17.833054	2
GCAGAGT	8485	0.0	17.76089	9
GTATAGG	700	0.0	17.65344	1
GAGTACT	3490	0.0	17.403042	12-13
AGAGTAC	7095	0.0	17.18669	10-11
CAGAGTA	7780	0.0	16.895569	10-11
>>END_MODULE