FastQCFastQC Report
Mon 15 Aug 2016
SRR2921377_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921377_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3208138
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.050.0513151
GTATCAA51250.042.9656641
TCAACGC60300.036.0472954
CAACGCA62450.034.7259455
TATCAAC64500.033.919712
AACGCAG66250.033.153546
ATCAACG66450.032.7804643
ACGCAGA80450.026.6555657
GTACATG91350.026.3907971
CGCAGAG83300.025.2325578
TACATGG96650.024.4042512
ACATGGG102750.021.846243
GCAGAGT89950.021.67929
CATGGGG88050.019.889394
GAGTACA72700.019.5832251
AGAGTAC76950.018.25138510-11
CAGAGTA85300.018.05004910-11
ATGGGGG54000.018.014845
TATACCG3208.38736E-917.7951455
AGTACAT75550.017.2118282
Produced by FastQC (version 0.11.5)