##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921377_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3208138
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.90543019034717	31.0	31.0	34.0	26.0	34.0
2	29.968247001843437	31.0	31.0	34.0	25.0	34.0
3	30.01370795146593	31.0	31.0	34.0	25.0	34.0
4	33.46683060392041	37.0	35.0	37.0	28.0	37.0
5	33.445180662427866	37.0	35.0	37.0	28.0	37.0
6	33.48212982109872	37.0	35.0	37.0	28.0	37.0
7	33.38979027710155	37.0	35.0	37.0	27.0	37.0
8	33.34680958238081	37.0	35.0	37.0	27.0	37.0
9	34.8423138281458	39.0	35.0	39.0	27.0	39.0
10-11	34.7198078760951	39.0	35.0	39.0	27.0	39.0
12-13	34.60039312523339	39.0	35.0	39.0	27.0	39.0
14-15	35.66555039091212	40.0	36.0	41.0	27.0	41.0
16-17	35.60771637629055	40.0	36.0	41.0	26.0	41.0
18-19	35.550551597219325	40.0	36.0	41.0	26.0	41.0
20-21	35.48851857370225	40.0	36.0	41.0	25.0	41.0
22-23	35.36615164310263	40.0	36.0	41.0	25.0	41.0
24-25	35.2614313972778	40.0	35.5	41.0	24.5	41.0
26-27	35.094483155026374	39.0	35.0	41.0	24.0	41.0
28-29	34.950039867362314	39.0	35.0	41.0	23.0	41.0
30-31	34.794300463384054	39.0	35.0	41.0	22.5	41.0
32-33	34.60971036782084	39.0	34.0	41.0	21.5	41.0
34-35	34.43895727054135	39.0	34.0	41.0	19.5	41.0
36-37	34.263248494921356	39.0	34.0	40.0	18.5	41.0
38-39	34.49451691292582	39.0	34.5	41.0	20.0	41.0
40-41	34.61148896961415	39.0	35.0	41.0	19.5	41.0
42-43	34.45158624722502	39.0	35.0	41.0	17.0	41.0
44-45	34.32978974096501	39.0	34.0	41.0	15.0	41.0
46-47	34.17026948341998	39.0	34.0	41.0	14.5	41.0
48-49	34.01923950902361	39.0	34.0	41.0	10.5	41.0
50-51	32.33082242721479	37.0	32.0	39.5	8.5	40.0
52-53	32.48220104621434	37.5	32.0	39.5	8.0	40.0
54-55	33.327141787541564	38.0	33.0	40.0	7.0	41.0
56-57	33.338472347511235	38.0	33.0	40.0	4.0	41.0
58-59	33.158099339866304	38.0	33.0	40.0	2.0	41.0
60-61	32.91255332532454	37.5	33.0	40.0	2.0	41.0
62-63	32.62732416747659	37.0	32.5	40.0	2.0	41.0
64-65	32.33926314890444	36.5	32.0	40.0	2.0	41.0
66-67	31.9976593899639	36.0	32.0	39.0	2.0	41.0
68-69	31.630780222047804	35.5	32.0	39.0	2.0	41.0
70-71	31.248313040149768	35.0	31.0	38.5	2.0	40.0
72-73	30.895813085347328	35.0	31.0	37.0	2.0	40.0
74-75	30.502018148845217	35.0	31.0	37.0	2.0	39.0
76-77	30.130146053567522	35.0	31.0	36.5	2.0	39.0
78-79	29.79125134267915	35.0	30.0	36.0	2.0	38.0
80-81	29.448561283835048	35.0	30.0	35.5	2.0	37.0
82-83	29.15645617489023	35.0	30.0	35.0	2.0	37.0
84-85	28.924074650155326	34.5	30.0	35.0	2.0	36.0
86-87	28.70103686312746	34.0	29.0	35.0	2.0	36.0
88-89	28.498715765967674	34.0	29.0	35.0	2.0	36.0
90-91	28.25116921404254	34.0	29.0	35.0	2.0	35.0
92-93	28.051639455659327	34.0	29.0	35.0	2.0	35.0
94-95	27.8426841364056	34.0	29.0	35.0	2.0	35.0
96-97	27.58254118120854	34.0	29.0	35.0	2.0	35.0
98-99	27.337743108307684	34.0	28.5	35.0	2.0	35.0
100-101	26.29737592335492	33.0	25.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	138378.0
3	22593.0
4	15295.0
5	11609.0
6	10357.0
7	10825.0
8	11505.0
9	11962.0
10	13081.0
11	13884.0
12	13768.0
13	14287.0
14	14705.0
15	15225.0
16	15818.0
17	16596.0
18	15340.0
19	14817.0
20	14837.0
21	15486.0
22	16813.0
23	19597.0
24	22848.0
25	27150.0
26	32040.0
27	37868.0
28	44349.0
29	52296.0
30	62222.0
31	74776.0
32	92808.0
33	119135.0
34	164520.0
35	235357.0
36	380339.0
37	656956.0
38	649849.0
39	108837.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87466750912761	18.272566263493385	13.268541599679898	24.584224627699104
2	15.926208248238108	19.393508601427477	38.92166207349982	25.758621076834594
3	19.132426641883907	23.577917561975095	29.236620271757946	28.05303552438306
4	13.60967470292861	15.744440632504755	36.264541683560495	34.38134298100615
5	14.620082193951028	36.99039656803767	33.9931365743123	14.396384663699003
6	33.711844168638564	34.82234214685884	17.07398435575841	14.39182932874419
7	29.26575378851292	31.584704973131476	20.748197204376904	18.401344033978695
8	27.011351626047947	34.31751505080328	19.801899939827962	18.86923338332081
9	27.05503678440941	15.61400516855481	19.272804478790626	38.05815356824516
10-11	25.814202838996437	25.097126926131157	27.592309162465185	21.496361072407222
12-13	26.324326945097525	22.577347665356033	27.40775627267635	23.69056911687009
14-15	23.63617028873817	24.188770276357687	24.608584104423933	27.566475330480205
16-17	23.818757633327753	27.181163480507482	25.492750112015234	23.50732877414953
18-19	23.85618938376633	26.661602290474036	26.69039534247831	22.791812983281325
20-21	24.29065511986562	25.795548349631108	26.699332369367717	23.214464161135556
22-23	24.199798882580513	25.517805627763746	26.351621171997806	23.93077431765793
24-25	23.976275553838335	25.818289602709548	26.32438076803677	23.88105407541535
26-27	23.81024319440742	26.006367113019373	26.316226004449494	23.867163688123714
28-29	23.909305933082035	26.204810684576557	26.19025089755475	23.69563248478666
30-31	24.257453830813418	25.8404975756871	26.103083411764082	23.798965181735397
32-33	23.96942395665822	25.887121273406382	26.028869269037664	24.114585500897732
34-35	24.279943344710215	25.980552839537	26.078002182478727	23.661501633274057
36-37	24.300172579080822	25.8428484572384	26.084861278244766	23.77211768543601
38-39	24.132218317460314	26.016126093450666	26.031837274558967	23.819818314530053
40-41	24.249328828986794	25.827704848406917	26.097404545649372	23.825561776956917
42-43	24.08396566781848	25.786766142746885	25.99163017566759	24.137638013767052
44-45	24.052718209473596	25.81728997357687	26.072338790782513	24.05765302616702
46-47	24.22843244234317	25.91527136617086	25.828750788094705	24.02754540339126
48-49	23.936891915106273	25.970921243733702	25.90114472225259	24.19104211890744
50-51	24.0364708471067	25.9312258046985	26.020132239928373	24.012171108266426
52-53	24.184237355475982	25.852088867121616	26.02149150534867	23.94218227205373
54-55	23.813134397167886	25.711691445709484	26.25228677367898	24.222887383443652
56-57	23.894519771919953	25.89547596255144	26.041075587947127	24.168928677581484
58-59	23.9884129444909	25.799175405479787	26.14267315688381	24.069738493145508
60-61	24.045639491749125	25.765632931657905	26.231586839207477	23.95714073738549
62-63	23.996830641752876	25.778196776975705	26.263549662494274	23.96142291877715
64-65	23.801957576930413	25.61399776681228	26.28070535494924	24.303339301308068
66-67	23.81243084496231	25.803970992560732	26.128934272553423	24.25466388992353
68-69	23.85756304896397	25.728612483949426	26.140203691967667	24.273620775118932
70-71	23.816917616674967	25.534052742441443	26.18028739930641	24.468742241577182
72-73	23.788189917003052	25.750322071878635	26.070301459278404	24.39118655183991
74-75	23.70137662736368	25.827913537488996	26.173596806540576	24.297113028606745
76-77	23.719504949211807	25.505199994565853	26.235671146038168	24.53962391018417
78-79	23.687997499586466	25.50050053568017	26.261511578704688	24.549990386028675
80-81	23.784548031587573	25.626234939189718	26.11878129183016	24.47043573739255
82-83	23.762791657443902	25.656591943647744	26.218814879912948	24.36180151899541
84-85	23.76099317046137	25.67383568026644	26.184375831277613	24.380795317994583
86-87	23.558656870081723	25.77946435406437	26.206687528414108	24.4551912474398
88-89	23.520386442939284	25.54910251533172	26.260825549977383	24.669685491751608
90-91	23.5893221034845	25.45605117671577	26.340970430411577	24.613656289388157
92-93	23.63628580485513	25.3686078228364	26.462522573445423	24.532583798863044
94-95	23.53513235472886	25.25630476759524	26.526523914538725	24.682038963137177
96-97	23.49279320425106	25.091449204461064	26.555714600669596	24.860042990618282
98-99	23.28503538526964	25.093388427427936	26.547295086807882	25.074281100494538
100-101	23.46737836799419	25.003082000911554	26.687206047815465	24.84233358327879
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	40.5
2	43.5
3	70.5
4	81.0
5	109.5
6	173.0
7	322.5
8	479.0
9	546.0
10	502.5
11	448.0
12	491.0
13	562.0
14	650.5
15	731.5
16	836.0
17	938.0
18	1072.0
19	1237.5
20	1406.0
21	1576.5
22	1697.5
23	1872.5
24	2241.0
25	2779.5
26	3691.0
27	5135.5
28	7063.0
29	10152.5
30	14699.0
31	20539.0
32	28006.5
33	34728.0
34	40682.0
35	47630.0
36	56538.0
37	65141.5
38	72900.5
39	80934.5
40	87123.5
41	92832.5
42	99336.0
43	106974.0
44	114279.5
45	121558.0
46	127436.5
47	131528.5
48	136673.0
49	139657.0
50	138960.0
51	137173.5
52	135831.5
53	134818.0
54	132993.0
55	127604.5
56	118300.5
57	107084.5
58	96920.0
59	86525.5
60	72483.5
61	59514.0
62	49068.5
63	37674.0
64	26981.5
65	19521.5
66	14678.0
67	11311.0
68	8570.5
69	6466.5
70	5006.0
71	3703.0
72	2656.0
73	2036.0
74	1576.0
75	1182.0
76	889.5
77	595.5
78	384.0
79	304.0
80	214.0
81	156.0
82	129.0
83	101.0
84	83.0
85	72.0
86	62.5
87	45.5
88	42.5
89	46.5
90	36.5
91	29.5
92	25.5
93	16.5
94	17.0
95	16.0
96	14.5
97	14.5
98	9.0
99	8.0
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05171223931140119
2	0.06003482393837173
3	0.050122532135463
4	0.05729179979165485
5	0.04912506880938414
6	0.017704974037899866
7	0.01499312062012295
8	0.02082204693189632
9	0.020728534745076427
10-11	0.022972827228753875
12-13	0.028505631615597587
14-15	0.046085922737737595
16-17	0.030703168005865086
18-19	0.02390794909695281
20-21	0.02806924141043808
22-23	0.04842372740823493
24-25	0.04374811806724025
26-27	0.03219936299498338
28-29	0.020993485941066127
30-31	0.013247559799484934
32-33	0.010364267372538214
34-35	0.009896706438438745
36-37	0.001355926708888458
38-39	0.007216023749601794
40-41	0.003911926481965551
42-43	0.22550463851617356
44-45	0.1994303237578932
46-47	0.2053371768920165
48-49	0.20223568936249
50-51	0.2012849821298211
52-53	0.20553978663012626
54-55	0.20494754278026694
56-57	0.21487541994764564
58-59	0.2121791518943387
60-61	0.21704178560897316
62-63	0.2138000297992169
64-65	0.21459488338718596
66-67	0.204028006276538
68-69	0.20382539653842818
70-71	0.18618276395840827
72-73	0.19366373890399977
74-75	0.19383517791316957
76-77	0.19212078782147152
78-79	0.2202991267831995
80-81	0.22824766266289045
82-83	0.2266891262158922
84-85	0.22182649250125774
86-87	0.24010812502454695
88-89	0.2389392226892983
90-91	0.23752095452252991
92-93	0.2416199053781352
94-95	0.25369856284237147
96-97	0.25365180674896154
98-99	0.24297583208702367
100-101	0.25268551415182267
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3208138.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.72083199753817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26264120466855	45.17384764658281
2	11.727500764296881	13.538422027339617
3	3.936630272346337	6.816767237595775
4	1.8056425052019636	4.168927507615059
5	1.0634703942666741	3.0692197980911184
6	0.6524361373921327	2.259549400532041
7	0.46629908952169863	1.884061998482088
8	0.34395279515770644	1.5882593203505306
9	0.2558610285392954	1.329166029873059
>10	1.3862394620432987	14.275360764014868
>50	0.07259960387019784	2.8533020538922313
>100	0.02525973542331399	2.3551322446313723
>500	0.001141005656506037	0.47127388144434806
>1k	3.260016154541082E-4	0.21671008955496435
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.117072893996455E-5
5	0.0	0.0	0.0	0.0	3.117072893996455E-5
6	0.0	0.0	0.0	0.0	6.23414578799291E-5
7	0.0	0.0	0.0	0.0	6.23414578799291E-5
8	0.0	0.0	0.0	0.0	6.23414578799291E-5
9	0.0	0.0	0.0	0.0	6.23414578799291E-5
10-11	6.23414578799291E-5	0.0	0.0	0.0	6.23414578799291E-5
12-13	6.23414578799291E-5	0.0	0.0	0.0	9.351218681989366E-5
14-15	9.351218681989364E-5	0.0	0.0	0.0	1.246829157598582E-4
16-17	1.246829157598582E-4	0.0	0.0	0.0	1.5585364469982276E-4
18-19	1.246829157598582E-4	0.0	0.0	1.5585364469982275E-5	1.870243736397873E-4
20-21	1.246829157598582E-4	0.0	0.0	3.117072893996455E-5	1.870243736397873E-4
22-23	1.4026828022984048E-4	3.117072893996455E-5	0.0	6.23414578799291E-5	1.870243736397873E-4
24-25	1.870243736397873E-4	3.117072893996455E-5	0.0	2.1819510257975187E-4	1.870243736397873E-4
26-27	1.870243736397873E-4	3.117072893996455E-5	0.0	4.363902051595037E-4	1.870243736397873E-4
28-29	1.870243736397873E-4	3.117072893996455E-5	0.0	0.0019014144653378378	1.870243736397873E-4
30-31	1.870243736397873E-4	3.117072893996455E-5	0.0	0.0080888041599208	1.870243736397873E-4
32-33	1.870243736397873E-4	4.675609340994682E-5	0.0	0.024188485657412495	2.493658315197164E-4
34-35	2.1819510257975184E-4	6.23414578799291E-5	0.0	0.046600239765247006	2.8053656045968096E-4
36-37	2.493658315197164E-4	6.23414578799291E-5	0.0	0.0827426999711359	2.9612192492966323E-4
38-39	2.493658315197164E-4	6.23414578799291E-5	0.0	0.1384915486802625	3.272926538696278E-4
40-41	2.493658315197164E-4	6.23414578799291E-5	0.0	0.19846403116075428	3.4287801833961007E-4
42-43	2.493658315197164E-4	6.23414578799291E-5	0.0	0.265574610568498	4.052194762195392E-4
44-45	2.493658315197164E-4	6.23414578799291E-5	0.0	0.35196428582560974	4.2080484068952146E-4
46-47	2.493658315197164E-4	6.23414578799291E-5	0.0	0.44970010641686864	4.6756093409946824E-4
48-49	2.493658315197164E-4	6.23414578799291E-5	0.0	0.5562572432981374	5.299023919793974E-4
50-51	2.493658315197164E-4	6.23414578799291E-5	0.0	0.6660561359891626	5.922438498593265E-4
52-53	2.493658315197164E-4	6.23414578799291E-5	0.0	0.7833048328968393	7.013414011492025E-4
54-55	2.493658315197164E-4	6.23414578799291E-5	0.0	0.9202846012235134	7.792682234991138E-4
56-57	2.493658315197164E-4	6.23414578799291E-5	0.0	1.0836348062333978	7.792682234991138E-4
58-59	2.493658315197164E-4	6.23414578799291E-5	0.0	1.2558686689911718	7.94853587969096E-4
60-61	2.493658315197164E-4	6.23414578799291E-5	0.0	1.4337600190515496	8.104389524390784E-4
62-63	2.493658315197164E-4	6.23414578799291E-5	0.0	1.621392221905666	8.727804103190075E-4
64-65	2.493658315197164E-4	6.23414578799291E-5	0.0	1.8305322277283582	8.727804103190075E-4
66-67	2.493658315197164E-4	6.23414578799291E-5	0.0	2.073741840282432	8.727804103190075E-4
68-69	2.8053656045968096E-4	6.23414578799291E-5	0.0	2.337508548572412	8.727804103190075E-4
70-71	2.8053656045968096E-4	6.23414578799291E-5	0.0	2.6124032070939593	9.03951139258972E-4
72-73	2.8053656045968096E-4	6.23414578799291E-5	0.0	2.901246766816141	0.001013048690548848
74-75	2.8053656045968096E-4	6.23414578799291E-5	0.0	3.2194063971063587	0.0010286340550188303
76-77	2.8053656045968096E-4	6.23414578799291E-5	0.0	3.5802543406798586	0.0010286340550188303
78-79	2.8053656045968096E-4	6.23414578799291E-5	0.0	3.9743614520323005	0.0010286340550188303
80-81	2.8053656045968096E-4	6.23414578799291E-5	0.0	4.3891035859429985	0.001075390148428777
82-83	2.8053656045968096E-4	6.23414578799291E-5	0.0	4.813399548273797	0.0010909755128987594
84-85	2.8053656045968096E-4	6.23414578799291E-5	0.0	5.267634995751429	0.0010909755128987594
86-87	2.8053656045968096E-4	6.23414578799291E-5	0.0	5.766039366136993	0.0011065608773687416
88-89	2.8053656045968096E-4	6.23414578799291E-5	0.0	6.3108257811852235	0.0011533169707786885
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2835	0.0	50.051315	1
GTATCAA	5125	0.0	42.965664	1
TCAACGC	6030	0.0	36.047295	4
CAACGCA	6245	0.0	34.725945	5
TATCAAC	6450	0.0	33.91971	2
AACGCAG	6625	0.0	33.15354	6
ATCAACG	6645	0.0	32.780464	3
ACGCAGA	8045	0.0	26.655565	7
GTACATG	9135	0.0	26.390797	1
CGCAGAG	8330	0.0	25.232557	8
TACATGG	9665	0.0	24.404251	2
ACATGGG	10275	0.0	21.84624	3
GCAGAGT	8995	0.0	21.6792	9
CATGGGG	8805	0.0	19.88939	4
GAGTACA	7270	0.0	19.583225	1
AGAGTAC	7695	0.0	18.251385	10-11
CAGAGTA	8530	0.0	18.050049	10-11
ATGGGGG	5400	0.0	18.01484	5
TATACCG	320	8.38736E-9	17.795145	5
AGTACAT	7555	0.0	17.211828	2
>>END_MODULE