FastQCFastQC Report
Mon 15 Aug 2016
SRR2921377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3208138
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29000.052.2832261
GTATCAA50950.043.5654681
TCAACGC67350.031.9902134
CAACGCA68900.031.261135
TATCAAC71100.030.6366982
ATCAACG71300.030.4176433
AACGCAG73650.029.0499326
GTACATG98200.025.6043551
ACGCAGA85650.024.53587
TACATGG102950.024.293112
CGCAGAG89650.023.0698248
ACATGGG108550.022.340353
CATGGGG89150.020.9204044
GCAGAGT101500.019.5332899
AGAGTAC85600.019.2735210-11
ATGGGGG54950.018.0844085
GTATATG10850.017.5226551
CAGAGTA91350.017.27964610-11
TAAGTAC19850.016.5218351
TACACCG4409.458745E-1116.2093015
Produced by FastQC (version 0.11.5)