##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921377_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3208138
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51572625616479	33.0	31.0	34.0	30.0	34.0
2	31.65917769123398	34.0	31.0	34.0	30.0	34.0
3	31.732202604750793	34.0	31.0	34.0	29.0	34.0
4	35.230576116114705	37.0	35.0	37.0	33.0	37.0
5	34.99091529105045	37.0	35.0	37.0	32.0	37.0
6	34.946537524258616	37.0	35.0	37.0	32.0	37.0
7	34.8182659224759	37.0	35.0	37.0	32.0	37.0
8	34.77876169915384	37.0	35.0	37.0	32.0	37.0
9	36.409732062648175	39.0	37.0	39.0	32.0	39.0
10-11	36.26313737127268	39.0	37.0	39.0	32.0	39.0
12-13	36.20558046443139	39.0	37.0	39.0	32.0	39.0
14-15	37.461188701982266	40.0	38.0	41.0	32.0	41.0
16-17	37.43996720215901	40.0	38.0	41.0	32.0	41.0
18-19	37.400552438829	40.0	38.0	41.0	32.0	41.0
20-21	37.36380557818897	40.0	38.0	41.0	31.5	41.0
22-23	37.25655769795439	40.0	38.0	41.0	31.0	41.0
24-25	37.19200296246608	40.0	38.0	41.0	31.0	41.0
26-27	37.058881195260305	40.0	37.0	41.0	31.0	41.0
28-29	36.91084174059844	40.0	37.0	41.0	30.0	41.0
30-31	36.775055343629234	40.0	37.0	41.0	30.0	41.0
32-33	36.73194419940788	40.0	37.0	41.0	30.0	41.0
34-35	36.85980886732429	40.0	37.0	41.0	30.0	41.0
36-37	36.835751298728425	40.0	37.0	41.0	30.0	41.0
38-39	36.749924878543254	40.0	37.0	41.0	30.0	41.0
40-41	36.63045651402776	40.0	37.0	41.0	30.0	41.0
42-43	36.52974513565189	40.0	37.0	41.0	30.0	41.0
44-45	36.39746700422488	40.0	36.0	41.0	30.0	41.0
46-47	36.24341456009685	40.0	36.0	41.0	29.0	41.0
48-49	36.08527547755115	40.0	36.0	41.0	28.5	41.0
50-51	35.93044127154131	40.0	35.0	41.0	28.0	41.0
52-53	35.762533594253114	39.0	35.0	41.0	28.0	41.0
54-55	35.550287892852495	39.0	35.0	41.0	28.0	41.0
56-57	35.27960814029821	39.0	35.0	41.0	27.0	41.0
58-59	35.05020061481146	38.5	35.0	40.5	26.5	41.0
60-61	34.77531717775233	38.0	34.5	40.0	26.0	41.0
62-63	34.474427222270364	37.5	34.0	40.0	26.0	41.0
64-65	34.154354644345105	37.0	34.0	40.0	26.0	41.0
66-67	33.81423757332135	36.5	34.0	39.0	25.5	41.0
68-69	33.44558105044109	36.0	34.0	39.0	25.0	41.0
70-71	33.06677300041332	35.0	33.5	38.5	24.0	40.0
72-73	32.67122923016404	35.0	33.0	37.5	23.0	40.0
74-75	32.26309949883702	35.0	33.0	37.0	22.5	39.0
76-77	29.508819757753564	33.0	29.0	34.5	17.5	36.5
78-79	31.166319528648707	34.5	31.5	36.0	20.0	37.0
80-81	31.36596711238731	35.0	32.0	35.5	20.0	37.0
82-83	31.207860291546062	35.0	32.0	35.0	20.0	37.0
84-85	30.980085021280257	35.0	32.0	35.0	19.5	36.0
86-87	30.74347082326259	35.0	32.0	35.0	18.0	36.0
88-89	30.469001021776496	35.0	32.0	35.0	13.5	36.0
90-91	30.2862965994605	35.0	32.0	35.0	9.0	35.0
92-93	30.146876630618756	34.0	32.0	35.0	7.0	35.0
94-95	30.053407926965736	34.0	31.5	35.0	2.0	35.0
96-97	29.862956487532642	34.0	31.0	35.0	2.0	35.0
98-99	29.59840801736085	34.0	31.0	35.0	2.0	35.0
100-101	28.512329737685846	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	49813.0
3	17350.0
4	9667.0
5	6919.0
6	6155.0
7	6378.0
8	6607.0
9	7338.0
10	8145.0
11	8866.0
12	9665.0
13	10410.0
14	11218.0
15	12425.0
16	13534.0
17	14192.0
18	13385.0
19	11893.0
20	11248.0
21	11439.0
22	11960.0
23	13573.0
24	15984.0
25	19393.0
26	23656.0
27	28533.0
28	34711.0
29	41735.0
30	51039.0
31	63439.0
32	81176.0
33	106203.0
34	152662.0
35	228193.0
36	396960.0
37	773918.0
38	812568.0
39	105784.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.463051797199576	19.913231643600348	14.036136622696581	25.587579936503495
2	15.225029550090522	20.89618442248899	37.765826130758526	26.112959896661962
3	17.82929570176546	24.59331441156254	29.209135443406915	28.36825444326508
4	13.27780160697453	16.971579942244677	35.384206313185466	34.366412137595326
5	14.411090044408443	37.28609943712306	34.061023180751995	14.241787337716497
6	33.634436689743744	35.542643753760025	16.77128758856516	14.051631967931074
7	29.162675510849763	31.779678847679417	20.75875859338837	18.298887048082452
8	26.88107119556594	35.0822408051672	19.52910137188018	18.50758662738667
9	26.707392263051027	15.741186943953158	19.29200053114922	38.25942026184659
10-11	25.740439992879132	25.462283037028357	27.53084526294554	21.26643170714697
12-13	26.26126356396807	22.742658344482088	27.515014968212032	23.481063123337815
14-15	23.51636701375624	24.376399155663183	24.602025304854813	27.50520852572576
16-17	23.747816879391372	27.52207901345859	25.41033939635371	23.319764710796328
18-19	23.782217406672927	26.828154505153478	26.7144900169133	22.675138071260292
20-21	24.301997525652883	25.907751551610147	26.692323445607848	23.097927477129122
22-23	24.183092102761293	25.629135191983877	26.378948011523207	23.80882469373162
24-25	23.958538543830468	25.973243252678852	26.29912271733194	23.769095486158733
26-27	23.742905743764208	26.146388479242706	26.407341150535856	23.703364626457233
28-29	23.896099280886595	26.328572027537568	26.226309135687632	23.549019555888208
30-31	24.195722397438967	25.98714714626711	26.113798899462516	23.703331556831408
32-33	23.940373375649138	26.008256881448983	26.015209060278977	24.036160682622903
34-35	24.26180426561065	26.06914989710143	26.12928512186268	23.539760715425246
36-37	24.251905401205754	25.992724080823738	26.135549295739523	23.619821222230986
38-39	24.16550698296095	26.05743438429328	26.114211999792403	23.662846632953364
40-41	24.233064003291812	25.934690582176835	26.08723300789287	23.745012406638484
42-43	24.105406884039336	25.88304334204632	25.970983725885365	24.040566048028982
44-45	24.019839428502756	25.94042217313784	26.10066946614942	23.939068932209988
46-47	24.255201285112317	25.968253136950313	25.830199578342004	23.946345999595366
48-49	23.97922458010065	26.07598575068436	25.92742288712932	24.017366782085666
50-51	23.998902095059492	26.026488125668656	26.02873256507004	23.945877214201815
52-53	24.25566495115539	25.907571238448902	25.928393652093938	23.908370158301775
54-55	23.772150509848636	25.83775987661267	26.206949327469857	24.183140286068834
56-57	23.89576022676298	25.959738535523364	25.968186348147764	24.17631488956589
58-59	23.954258181658915	25.901742626363834	26.09930605747405	24.04469313450321
60-61	23.999261194309014	25.953885432123396	26.148920782572915	23.897932590994674
62-63	23.967004759234726	25.87758863570008	26.157318965996986	23.99808763906822
64-65	23.742034942031697	25.750591391571298	26.154825245408208	24.352548420988793
66-67	23.77384195123229	25.968805216498957	26.00022108037207	24.257131751896686
68-69	23.76598528568785	25.8386881991017	26.051391433659397	24.34393508155105
70-71	23.74431566057146	25.72948048185798	26.04884501457355	24.47735884299701
72-73	23.80065123038331	25.93187413703088	25.84232879012295	24.425145842462857
74-75	23.676974712887368	25.988030673153816	25.922603133725037	24.412391480233776
76-77	23.708972339920255	25.665098053920282	26.05739185321284	24.568537752946618
78-79	23.614608835179514	25.724155603443638	26.05445755570005	24.6067780056768
80-81	23.76298408258915	25.8685838999559	25.84591068061449	24.52252133684046
82-83	23.703143209354614	25.9349222412818	25.906317284003734	24.455617265359848
84-85	23.650350095163024	25.97171051482822	25.8757212060136	24.50221818399515
86-87	23.52846028101525	26.03473071451746	25.83036449624398	24.606444508223316
88-89	23.428692064617167	25.894120930447695	25.87213454231903	24.80505246261611
90-91	23.361617829395552	25.826621824248363	25.939040578636057	24.872719767720024
92-93	23.449824491368034	25.755342058604757	25.99370944627845	24.801124003748757
94-95	23.3536092409136	25.73370995492667	26.001478493806644	24.91120231035309
96-97	23.21282165905714	25.648025358015502	26.03030966182039	25.10884332110697
98-99	23.043356557908492	25.684112944251048	25.9399032946645	25.332627203175967
100-101	23.05379641533868	25.682202048542656	25.961698963447205	25.302302572671458
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	34.5
2	38.0
3	52.5
4	49.5
5	53.5
6	95.0
7	249.0
8	394.0
9	420.5
10	378.5
11	306.0
12	283.5
13	316.0
14	364.0
15	399.5
16	441.0
17	485.5
18	592.0
19	695.5
20	761.0
21	881.0
22	1064.0
23	1321.0
24	1640.5
25	2176.0
26	3101.0
27	4637.0
28	6816.5
29	10020.0
30	14758.5
31	20541.5
32	28240.0
33	35120.5
34	41018.5
35	48389.0
36	57368.0
37	65968.5
38	73887.0
39	81935.5
40	88176.5
41	94389.5
42	100977.0
43	108021.5
44	115209.5
45	123185.5
46	129361.5
47	133512.0
48	139249.5
49	142273.0
50	141060.5
51	139091.0
52	137291.0
53	136378.0
54	134480.0
55	128198.0
56	118123.0
57	106470.0
58	96274.5
59	85001.0
60	70722.5
61	57953.5
62	47216.5
63	35754.5
64	25125.0
65	17894.0
66	13352.0
67	10168.5
68	7554.5
69	5585.5
70	4119.0
71	3020.0
72	2192.0
73	1648.0
74	1300.5
75	952.0
76	657.5
77	377.5
78	190.0
79	102.0
80	61.5
81	44.5
82	28.0
83	21.0
84	16.5
85	13.5
86	9.5
87	5.5
88	3.5
89	2.0
90	3.0
91	2.5
92	1.0
93	0.0
94	0.0
95	0.0
96	1.0
97	2.0
98	1.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15981232727519826
2	8.104389524390784E-4
3	0.0060159506854131585
4	0.0038028289306756757
5	0.026962680533069338
6	0.0014961949891182985
7	9.351218681989366E-5
8	0.0018390730074579085
9	0.0
10-11	0.1082403562440269
12-13	1.870243736397873E-4
14-15	0.005501633657903743
16-17	3.117072893996456E-5
18-19	3.740487472795746E-4
20-21	5.922438498593265E-4
22-23	1.2468291575985823E-4
24-25	0.0015585364469982274
26-27	0.002649511959896987
28-29	0.007153682291721865
30-31	0.016317876600071442
32-33	0.015912657123851905
34-35	0.011455242885436975
36-37	6.545853077392556E-4
38-39	2.3378046704973415E-4
40-41	0.00554838975131369
42-43	0.008821316290009968
44-45	0.00883690165447995
46-47	0.01013048690548848
48-49	0.012624145220685643
50-51	0.006545853077392557
52-53	0.0017611461851079972
54-55	9.03951139258972E-4
56-57	0.0064523408905726625
58-59	0.009584999149039099
60-61	0.008042048066510855
62-63	0.01821929106540928
64-65	0.03377348480645159
66-67	0.0361424602058889
68-69	0.02657304642131978
70-71	0.04055311835089388
72-73	0.025700266011000773
74-75	0.0240949734705926
76-77	0.24493958801024146
78-79	0.07117835953440906
80-81	0.05336428794521931
82-83	0.02032331526885689
84-85	0.0314824362293642
86-87	0.0816049683648272
88-89	0.2628004156928411
90-91	0.3202168984002558
92-93	0.05433058054235821
94-95	0.004472999602884913
96-97	0.009849950345028799
98-99	0.0013091706154785112
100-101	0.0016364632693481392
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3208138.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.61817783135762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.56504452313814	42.584082619537114
2	12.570357362436793	13.982807423754501
3	4.282579712055205	7.145678400061521
4	1.9961077777021368	4.440795094031741
5	1.111455168318742	3.090855560156665
6	0.7048313909880702	2.352086258705859
7	0.5111687228099628	1.9901191048950724
8	0.3676764487630144	1.635959528936271
9	0.26886889584300255	1.34585982560852
>10	1.511436411294441	15.130103807910022
>50	0.08176187943563022	3.104797689905393
>100	0.02708185624372622	2.4619698558338694
>500	0.0011240351534756482	0.4109853578427927
>1k	5.058158176433519E-4	0.3238994728206919
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.117072893996455E-5
5	0.0	0.0	0.0	0.0	3.117072893996455E-5
6	0.0	0.0	0.0	0.0	3.117072893996455E-5
7	0.0	0.0	0.0	0.0	3.117072893996455E-5
8	0.0	0.0	0.0	0.0	3.117072893996455E-5
9	0.0	0.0	0.0	0.0	3.117072893996455E-5
10-11	3.117072893996455E-5	0.0	0.0	0.0	3.117072893996455E-5
12-13	3.117072893996455E-5	0.0	0.0	0.0	1.7143900916980503E-4
14-15	4.675609340994682E-5	0.0	0.0	0.0	2.026097381097696E-4
16-17	6.23414578799291E-5	0.0	0.0	0.0	2.1819510257975187E-4
18-19	6.23414578799291E-5	0.0	0.0	1.5585364469982275E-5	2.1819510257975187E-4
20-21	6.23414578799291E-5	0.0	0.0	3.117072893996455E-5	2.1819510257975187E-4
22-23	6.23414578799291E-5	0.0	0.0	6.23414578799291E-5	2.493658315197164E-4
24-25	7.792682234991138E-5	0.0	0.0	2.1819510257975187E-4	2.8053656045968096E-4
26-27	9.351218681989366E-5	0.0	0.0	4.6756093409946824E-4	2.8053656045968096E-4
28-29	9.351218681989366E-5	0.0	0.0	0.001994926652157731	2.8053656045968096E-4
30-31	1.246829157598582E-4	0.0	0.0	0.008603121187430217	3.117072893996455E-4
32-33	1.246829157598582E-4	0.0	0.0	0.025778192833350685	4.052194762195392E-4
34-35	1.5585364469982276E-4	0.0	0.0	0.04907831271597419	4.3639020515950374E-4
36-37	1.870243736397873E-4	0.0	0.0	0.08610913869665207	4.51975569629486E-4
38-39	1.870243736397873E-4	0.0	0.0	0.1434944506751268	4.831462985694506E-4
40-41	1.870243736397873E-4	0.0	0.0	0.20468259158427723	4.987316630394328E-4
42-43	1.870243736397873E-4	0.0	0.0	0.27314909770090934	4.987316630394328E-4
44-45	1.870243736397873E-4	0.0	0.0	0.3608167728445597	4.987316630394328E-4
46-47	1.870243736397873E-4	0.0	0.0	0.45992410550917695	4.987316630394328E-4
48-49	1.870243736397873E-4	0.0	0.0	0.5689904860701129	5.299023919793974E-4
50-51	1.870243736397873E-4	0.0	0.0	0.6799426957319168	5.922438498593265E-4
52-53	2.8053656045968096E-4	1.5585364469982275E-5	0.0	0.7980641730499125	6.701706722092378E-4
54-55	2.8053656045968096E-4	3.117072893996455E-5	0.0	0.936462209543355	7.792682234991138E-4
56-57	2.8053656045968096E-4	3.117072893996455E-5	0.0	1.1014177070936473	7.792682234991138E-4
58-59	2.8053656045968096E-4	3.117072893996455E-5	0.0	1.2753192038497096	7.792682234991138E-4
60-61	2.8053656045968096E-4	3.117072893996455E-5	0.0	1.4564367243553737	7.792682234991138E-4
62-63	2.8053656045968096E-4	3.117072893996455E-5	0.0	1.646219707506348	7.792682234991138E-4
64-65	2.8053656045968096E-4	3.117072893996455E-5	0.0	1.8588975910637262	7.94853587969096E-4
66-67	2.8053656045968096E-4	3.117072893996455E-5	0.0	2.1062684959312845	8.260243169090607E-4
68-69	2.8053656045968096E-4	3.117072893996455E-5	0.0	2.3734172283112507	8.416096813790429E-4
70-71	2.8053656045968096E-4	3.117072893996455E-5	0.0	2.6533459595566025	8.416096813790429E-4
72-73	2.8053656045968096E-4	3.117072893996455E-5	0.0	2.948454835795717	9.818779616088834E-4
74-75	2.8053656045968096E-4	3.117072893996455E-5	0.0	3.2733785142659073	9.974633260788656E-4
76-77	2.8053656045968096E-4	3.117072893996455E-5	0.0	3.6418477010652284	9.974633260788656E-4
78-79	2.8053656045968096E-4	3.117072893996455E-5	0.0	4.043653982465841	9.974633260788656E-4
80-81	2.8053656045968096E-4	3.117072893996455E-5	0.0	4.465955018144481	0.0010286340550188303
82-83	2.8053656045968096E-4	3.117072893996455E-5	0.0	4.897856638336631	0.0010598047839587947
84-85	2.8053656045968096E-4	3.117072893996455E-5	0.0	5.360726377730634	0.0010909755128987594
86-87	2.8053656045968096E-4	3.117072893996455E-5	0.0	5.8688716009099355	0.0011221462418387238
88-89	2.8053656045968096E-4	3.117072893996455E-5	0.0	6.4223390639679465	0.0011221462418387238
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2900	0.0	52.283226	1
GTATCAA	5095	0.0	43.565468	1
TCAACGC	6735	0.0	31.990213	4
CAACGCA	6890	0.0	31.26113	5
TATCAAC	7110	0.0	30.636698	2
ATCAACG	7130	0.0	30.417643	3
AACGCAG	7365	0.0	29.049932	6
GTACATG	9820	0.0	25.604355	1
ACGCAGA	8565	0.0	24.5358	7
TACATGG	10295	0.0	24.29311	2
CGCAGAG	8965	0.0	23.069824	8
ACATGGG	10855	0.0	22.34035	3
CATGGGG	8915	0.0	20.920404	4
GCAGAGT	10150	0.0	19.533289	9
AGAGTAC	8560	0.0	19.27352	10-11
ATGGGGG	5495	0.0	18.084408	5
GTATATG	1085	0.0	17.522655	1
CAGAGTA	9135	0.0	17.279646	10-11
TAAGTAC	1985	0.0	16.521835	1
TACACCG	440	9.458745E-11	16.209301	5
>>END_MODULE