FastQCFastQC Report
Mon 15 Aug 2016
SRR2921376_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921376_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972762
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.059.0982631
GTATCAA39850.043.8223531
TATCAAC52150.033.4898952
TCAACGC52250.033.0624734
CAACGCA53050.032.560585
ATCAACG53800.032.2830853
AACGCAG56350.030.5586856
GTACATG65950.026.4794651
ACGCAGA66250.025.4935427
TACATGG68600.025.1824252
CGCAGAG68050.024.0565918
ACATGGG72200.023.0041943
CATGGGG61150.022.1967724
GTGGTAT9750.021.9020161
GCAGAGT71950.021.301739
ACCGTCG1353.8239622E-421.0892268
TATTCCG2056.8977533E-720.8336265
ATGGGGG41750.020.0047155
CAGAGTA65800.019.65120910-11
TAAGTAC17150.019.0924281
Produced by FastQC (version 0.11.5)