##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921376_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1972762
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.113727859721546	31.0	31.0	34.0	26.0	34.0
2	30.176795274848157	31.0	31.0	34.0	26.0	34.0
3	30.210704078849854	31.0	31.0	34.0	26.0	34.0
4	33.686846664726914	37.0	35.0	37.0	28.0	37.0
5	33.67725148801528	37.0	35.0	37.0	28.0	37.0
6	33.71899093757889	37.0	35.0	37.0	29.0	37.0
7	33.63370289979227	37.0	35.0	37.0	28.0	37.0
8	33.59250887841514	37.0	35.0	37.0	28.0	37.0
9	35.10782648895305	39.0	35.0	39.0	28.0	39.0
10-11	34.996004586463044	39.0	35.0	39.0	27.5	39.0
12-13	34.871736428418636	39.0	35.0	39.0	27.0	39.0
14-15	35.94046798346683	40.0	36.0	41.0	27.0	41.0
16-17	35.882177373651764	40.0	36.0	41.0	27.0	41.0
18-19	35.84150546289922	40.0	36.0	41.0	27.0	41.0
20-21	35.78143029924543	40.0	36.0	41.0	27.0	41.0
22-23	35.66124854391964	40.0	36.0	41.0	26.5	41.0
24-25	35.55154904646379	40.0	36.0	41.0	26.0	41.0
26-27	35.38590286106484	40.0	35.0	41.0	25.0	41.0
28-29	35.23487526625107	39.0	35.0	41.0	24.5	41.0
30-31	35.06712999337984	39.0	35.0	41.0	24.0	41.0
32-33	34.85231442008717	39.0	35.0	41.0	24.0	41.0
34-35	34.677628877685194	39.0	34.5	41.0	23.0	41.0
36-37	34.495775212620686	39.0	34.0	40.5	21.5	41.0
38-39	34.711661112693776	39.0	35.0	41.0	23.0	41.0
40-41	34.81300937467368	39.0	35.0	41.0	22.5	41.0
42-43	34.62968214107936	39.0	35.0	41.0	20.0	41.0
44-45	34.4801694274322	39.0	35.0	41.0	18.5	41.0
46-47	34.291941957519455	39.0	34.0	41.0	15.5	41.0
48-49	34.11982819012126	39.0	34.0	41.0	14.0	41.0
50-51	32.41571081559762	37.0	32.0	39.5	10.5	40.0
52-53	32.56457139786756	37.5	32.0	39.5	8.0	40.0
54-55	33.41526803537376	38.0	33.0	40.0	8.0	41.0
56-57	33.43412687389558	38.0	33.0	41.0	7.0	41.0
58-59	33.25040349520114	38.0	33.0	40.0	3.5	41.0
60-61	33.00744666614625	37.5	33.0	40.0	2.0	41.0
62-63	32.71621741497454	37.0	33.0	40.0	2.0	41.0
64-65	32.43099370324448	36.5	32.5	40.0	2.0	41.0
66-67	32.08557950731006	36.0	32.0	39.0	2.0	41.0
68-69	31.718433597159716	35.5	32.0	39.0	2.0	41.0
70-71	31.327968857875405	35.0	31.5	38.5	2.0	40.5
72-73	30.965829126878965	35.0	31.0	37.5	2.0	40.0
74-75	30.562381574665366	35.0	31.0	37.0	2.0	39.0
76-77	30.175679073299264	35.0	31.0	36.5	2.0	39.0
78-79	29.821208285642157	35.0	30.0	36.0	2.0	38.5
80-81	29.474091147335564	35.0	30.0	35.5	2.0	37.0
82-83	29.16817462015185	35.0	30.0	35.0	2.0	37.0
84-85	28.92571607725615	34.5	30.0	35.0	2.0	36.5
86-87	28.692521703074167	34.0	29.5	35.0	2.0	36.0
88-89	28.478557474241697	34.0	29.0	35.0	2.0	36.0
90-91	28.210244317358097	34.0	29.0	35.0	2.0	35.5
92-93	27.999897605489156	34.0	29.0	35.0	2.0	35.0
94-95	27.76982499662909	34.0	29.0	35.0	2.0	35.0
96-97	27.492911968093466	34.0	29.0	35.0	2.0	35.0
98-99	27.24148452778389	34.0	27.5	35.0	2.0	35.0
100-101	26.178883463894785	33.0	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74422.0
3	12912.0
4	8770.0
5	6769.0
6	6233.0
7	6516.0
8	6782.0
9	7377.0
10	7935.0
11	8686.0
12	8651.0
13	8895.0
14	9643.0
15	10587.0
16	11333.0
17	12195.0
18	11061.0
19	9752.0
20	9756.0
21	10160.0
22	11103.0
23	12500.0
24	14470.0
25	16964.0
26	20120.0
27	23499.0
28	27302.0
29	32361.0
30	38027.0
31	45990.0
32	56897.0
33	73639.0
34	102628.0
35	145032.0
36	229004.0
37	396594.0
38	407078.0
39	71111.0
40	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.03904295018679	18.835680008277013	13.824081033571604	24.301196007964595
2	16.22218209631705	20.11379751791971	38.99299645156251	24.67102393420073
3	19.259266021452042	23.927410565351437	29.81797360411858	26.99534980907794
4	13.987596944833438	16.1813359734755	36.01729752978656	33.81376955190451
5	14.48862728670172	37.172383748499456	33.85999763665438	14.478991328144456
6	33.18872248304114	34.60708165603675	17.32617799454477	14.878017866377343
7	28.376532021952116	32.33931988998593	21.003916399447395	18.280231688614556
8	26.68138324328134	34.12903909441339	20.29589647831375	18.893681183991514
9	27.20181548915234	15.507607380190544	19.741771410000503	37.54880572065662
10-11	25.63154829944804	25.332534272533984	27.691056527213824	21.344860900804154
12-13	26.044931052867625	23.029947021079764	27.491336449481913	23.4337854765707
14-15	23.53920999835687	24.76767939045888	25.095621154614665	26.597489456569583
16-17	23.33410659618566	27.58548040539256	26.0811418937334	22.999271104688383
18-19	23.382316853048426	26.93315303585263	27.497064335170784	22.187465775928157
20-21	23.852695828888283	25.97719233842672	27.589071671081296	22.581040161603706
22-23	23.957001800635812	25.668404584939626	27.085660411902097	23.288933202522465
24-25	23.652349420426006	25.99070196724263	27.024337924030988	23.332610688300377
26-27	23.220128681830143	26.241474410428005	27.160547037219768	23.377849870522084
28-29	23.278288733179878	26.692583428820587	26.860176237362726	23.168951600636806
30-31	24.048886420492266	26.04658932451132	26.759216330826437	23.145307924169977
32-33	23.661652378149643	26.08634046162277	26.644979146420166	23.607028013807415
34-35	23.942820497221884	26.304370969704344	26.69807255546092	23.054735977612847
36-37	23.828632011904343	26.257429125339538	26.81571764546056	23.098221217295563
38-39	23.705266583748035	26.284277760020764	26.784704477530223	23.22575117870098
40-41	23.882382223555997	26.071614323270193	26.719312528451173	23.326690924722637
42-43	23.655326706042928	26.16470610948518	26.411063898321146	23.768903286150746
44-45	23.54091319699199	26.203056315532976	26.629659512339572	23.62637097513547
46-47	23.65986867269206	26.070964697468185	26.367738812365104	23.901427817474648
48-49	23.432757051310706	26.333359069279815	26.448510498234377	23.785373381175106
50-51	23.647907165625355	26.193959782166736	26.63238439957059	23.525748652637315
52-53	23.84748177246767	26.116999080337276	26.474196996784354	23.5613221504107
54-55	23.175280039924974	25.95908197953718	26.807513108319352	24.05812487221849
56-57	23.382452974636507	26.104963324102766	26.507460912317704	24.005122788943027
58-59	23.520157355437057	26.130115918573033	26.609126473111754	23.740600252878153
60-61	23.685893674600404	25.974419194189885	26.862868815690078	23.476818315519633
62-63	23.580610887547934	25.922525135299963	26.926006726453412	23.570857250698694
64-65	23.24425789162862	25.772042901503077	26.87396889704528	24.10973030982302
66-67	23.32415962256733	25.948089800774028	26.588852671525785	24.138897905132858
68-69	23.287956519530656	25.914512114593386	26.808325290801037	23.98920607507492
70-71	23.2374584827278	25.735807948970574	26.71691060709171	24.309822961209917
72-73	23.14201178773743	26.038004266826754	26.576880970465645	24.243102974970167
74-75	23.164006085633115	26.044041354066067	26.68155484827946	24.11039771202136
76-77	23.137521591468342	25.561606725212282	26.716994701234007	24.583876982085368
78-79	22.996880947923593	25.687231648865712	26.86880853937984	24.44707886383086
80-81	23.210941445574758	25.8346009980764	26.6322390261896	24.322218530159244
82-83	23.162951106107183	25.85300560426962	26.781072246836818	24.20297104278638
84-85	23.14680795611749	25.875671312019676	26.659704818306935	24.317815913555894
86-87	22.90556558691318	26.078006784060225	26.651407485433783	24.365020143592808
88-89	22.814955923739188	25.65759972195256	26.853808378465654	24.673635975842593
90-91	23.000651417468152	25.44928496516288	26.94137141161454	24.608692205754426
92-93	23.132199093882182	25.285626304653153	26.950475518761596	24.63169908270307
94-95	22.961002977331674	25.27161767168445	26.98285283616443	24.78452651481945
96-97	22.81523312641647	25.068500077628762	27.169965317349178	24.94630147860559
98-99	22.51196694987601	25.00279482905809	27.153822309849996	25.331415911215906
100-101	22.745328922476542	24.981088523497114	27.23809097887632	25.035491575150026
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	139.0
1	112.5
2	122.5
3	146.0
4	122.0
5	133.0
6	224.0
7	420.0
8	622.5
9	719.0
10	642.0
11	548.0
12	574.5
13	661.5
14	745.0
15	799.0
16	879.5
17	1003.0
18	1166.5
19	1299.5
20	1374.0
21	1546.0
22	1773.5
23	2000.0
24	2321.0
25	2825.0
26	3626.0
27	4885.5
28	6604.0
29	8900.5
30	11960.0
31	15843.0
32	21035.0
33	25087.5
34	28272.5
35	32338.5
36	37158.5
37	42551.5
38	47370.5
39	51513.5
40	55103.5
41	58495.5
42	62159.0
43	65989.5
44	70213.5
45	75419.5
46	79142.0
47	81393.0
48	84181.5
49	86104.0
50	85899.0
51	84355.0
52	82531.5
53	81327.5
54	79312.5
55	74259.0
56	67975.0
57	61300.5
58	54463.5
59	46943.5
60	38437.5
61	31579.5
62	26079.5
63	20060.5
64	14515.5
65	10778.5
66	8268.0
67	6415.5
68	4935.5
69	3807.5
70	2881.5
71	2119.0
72	1554.0
73	1144.5
74	931.0
75	745.5
76	547.5
77	368.0
78	243.0
79	165.5
80	115.0
81	91.5
82	70.5
83	58.0
84	50.0
85	38.5
86	32.5
87	30.5
88	29.5
89	21.0
90	15.0
91	13.0
92	9.0
93	10.0
94	12.0
95	7.5
96	6.5
97	5.0
98	2.5
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.052515204571053166
2	0.060321518764047566
3	0.05145070718109939
4	0.05834459503984769
5	0.04972723521641231
6	0.017336100350675853
7	0.014548130996035002
8	0.019414404778680856
9	0.020326831112926953
10-11	0.022633242124493476
12-13	0.02851332294519055
14-15	0.04653374304655098
16-17	0.030388865965585304
18-19	0.023621703986593415
20-21	0.02686588650835732
22-23	0.0485867022986047
24-25	0.042554550422199945
26-27	0.03097180501246476
28-29	0.02139132850288073
30-31	0.013762430541545306
32-33	0.010087380028609635
34-35	0.010239451084317317
36-37	0.001850197844443476
38-39	0.007375446201822622
40-41	0.004257989559815122
42-43	0.22742226381083983
44-45	0.20040430624677485
46-47	0.20747561033718206
48-49	0.20529592520537196
50-51	0.2031922756014157
52-53	0.2081092397359641
54-55	0.2062843870674719
56-57	0.21812058423671987
58-59	0.21416673678832013
60-61	0.21964129479379674
62-63	0.21621969604037386
64-65	0.21794316800506092
66-67	0.20747561033718206
68-69	0.2059042094282027
70-71	0.18765568274328073
72-73	0.1952338903527136
74-75	0.19579148422364176
76-77	0.193282311804465
78-79	0.22179563474965555
80-81	0.23195905030611902
82-83	0.2296526392945525
84-85	0.22379790364980673
86-87	0.24161556234355688
88-89	0.24090589741692106
90-91	0.24057641012955439
92-93	0.2436178312437081
94-95	0.25651852580290985
96-97	0.2566705968586175
98-99	0.24544268391220023
100-101	0.25555540911676117
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1972762.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.3217369670248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.02486921531106	52.11660325454823
2	10.854020871913592	13.962989511156469
3	3.2976598168866973	6.3633362204554
4	1.5724629727625419	4.045741988976725
5	0.8516041354489374	2.738832860018856
6	0.5548988400469648	2.1415234339684828
7	0.3676933746434056	1.6555473569831594
8	0.2702519558491427	1.3906460175162045
9	0.20044607140745144	1.1603735533029207
>10	0.9552255221948129	10.413131843933519
>50	0.03541726002958556	1.543770004716297
>100	0.013945536300626386	1.650583622572948
>500	0.0012668860675488403	0.5719970228812827
>1k	2.3754113766540658E-4	0.24492330896946665
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0138070380512195E-4
2	0.0	0.0	0.0	0.0	1.0138070380512195E-4
3	0.0	0.0	0.0	0.0	1.0138070380512195E-4
4	0.0	0.0	0.0	0.0	1.0138070380512195E-4
5	0.0	0.0	0.0	0.0	1.0138070380512195E-4
6	0.0	0.0	0.0	0.0	1.0138070380512195E-4
7	0.0	0.0	0.0	0.0	1.0138070380512195E-4
8	0.0	0.0	0.0	0.0	1.0138070380512195E-4
9	0.0	0.0	0.0	0.0	1.0138070380512195E-4
10-11	0.0	0.0	0.0	0.0	1.0138070380512195E-4
12-13	0.0	0.0	0.0	0.0	1.2672587975640244E-4
14-15	0.0	0.0	0.0	0.0	2.2810658356152438E-4
16-17	0.0	0.0	0.0	0.0	3.294872873666464E-4
18-19	0.0	0.0	0.0	0.0	4.055228152204878E-4
20-21	0.0	2.5345175951280488E-5	0.0	7.603552785384146E-5	4.055228152204878E-4
22-23	2.5345175951280488E-5	5.0690351902560976E-5	0.0	2.027614076102439E-4	5.069035190256098E-4
24-25	5.0690351902560976E-5	5.0690351902560976E-5	0.0	3.8017763926920733E-4	6.589745747332927E-4
26-27	5.0690351902560976E-5	1.0138070380512195E-4	0.0	0.0014446750292229878	6.589745747332927E-4
28-29	5.0690351902560976E-5	1.0138070380512195E-4	0.0	0.004486096143376647	6.589745747332927E-4
30-31	5.0690351902560976E-5	1.0138070380512195E-4	0.0	0.015866080145501588	6.589745747332927E-4
32-33	5.0690351902560976E-5	1.0138070380512195E-4	0.0	0.043847154395715245	7.096649266358537E-4
34-35	5.0690351902560976E-5	1.0138070380512195E-4	0.0	0.08766896361547921	7.096649266358537E-4
36-37	5.0690351902560976E-5	1.0138070380512195E-4	0.0	0.14814255343523447	7.096649266358537E-4
38-39	5.0690351902560976E-5	1.0138070380512195E-4	0.0	0.23299820252012154	7.096649266358537E-4
40-41	5.0690351902560976E-5	1.0138070380512195E-4	0.0	0.32667397283605426	7.096649266358537E-4
42-43	5.0690351902560976E-5	1.0138070380512195E-4	0.0	0.43355457982260404	7.096649266358537E-4
44-45	5.0690351902560976E-5	1.0138070380512195E-4	0.0	0.5730797734344031	7.096649266358537E-4
46-47	5.0690351902560976E-5	1.0138070380512195E-4	0.0	0.7312843617222959	7.857004544896952E-4
48-49	5.0690351902560976E-5	1.2672587975640244E-4	0.0	0.8849014731630069	8.617359823435367E-4
50-51	5.0690351902560976E-5	1.5207105570768292E-4	0.0	1.0391775591784513	8.617359823435367E-4
52-53	5.0690351902560976E-5	1.5207105570768292E-4	0.0	1.2003221878766928	8.870811582948172E-4
54-55	5.0690351902560976E-5	1.5207105570768292E-4	0.0	1.4049844836832825	9.124263342460976E-4
56-57	5.0690351902560976E-5	1.5207105570768292E-4	0.0	1.645560893812837	9.124263342460976E-4
58-59	5.0690351902560976E-5	1.5207105570768292E-4	0.0	1.892144110642845	9.124263342460976E-4
60-61	5.0690351902560976E-5	1.5207105570768292E-4	0.0	2.137206616915776	9.631166861486586E-4
62-63	5.0690351902560976E-5	1.5207105570768292E-4	0.0	2.396259660313814	9.631166861486586E-4
64-65	5.0690351902560976E-5	1.5207105570768292E-4	0.0	2.695814294881998	9.631166861486586E-4
66-67	1.0138070380512195E-4	1.5207105570768292E-4	0.0	3.0587572145043342	9.631166861486586E-4
68-69	1.0138070380512195E-4	1.5207105570768292E-4	0.0	3.4474001425412695	9.631166861486586E-4
70-71	1.0138070380512195E-4	1.5207105570768292E-4	0.0	3.841973841750804	0.0012165684456614634
72-73	1.0138070380512195E-4	1.5207105570768292E-4	0.0	4.246964408276315	0.0012165684456614634
74-75	1.0138070380512195E-4	1.5207105570768292E-4	0.0	4.6889842768666465	0.0012165684456614634
76-77	1.0138070380512195E-4	2.027614076102439E-4	0.0	5.212235434380832	0.0012165684456614634
78-79	1.0138070380512195E-4	2.281065835615244E-4	0.0	5.787875070586315	0.0012672587975640244
80-81	1.0138070380512195E-4	2.534517595128049E-4	0.0	6.371118259577182	0.001292603973515305
82-83	1.2672587975640244E-4	2.534517595128049E-4	0.0	6.955248529726344	0.0014193298532717074
84-85	1.5207105570768292E-4	2.534517595128049E-4	0.0	7.583960964373807	0.0014193298532717074
86-87	1.5207105570768292E-4	2.534517595128049E-4	0.0	8.28310764298988	0.0014193298532717074
88-89	1.5207105570768292E-4	2.534517595128049E-4	0.0	9.038698028449453	0.001444675029222988
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2160	0.0	59.098263	1
GTATCAA	3985	0.0	43.822353	1
TATCAAC	5215	0.0	33.489895	2
TCAACGC	5225	0.0	33.062473	4
CAACGCA	5305	0.0	32.56058	5
ATCAACG	5380	0.0	32.283085	3
AACGCAG	5635	0.0	30.558685	6
GTACATG	6595	0.0	26.479465	1
ACGCAGA	6625	0.0	25.493542	7
TACATGG	6860	0.0	25.182425	2
CGCAGAG	6805	0.0	24.056591	8
ACATGGG	7220	0.0	23.004194	3
CATGGGG	6115	0.0	22.196772	4
GTGGTAT	975	0.0	21.902016	1
GCAGAGT	7195	0.0	21.30173	9
ACCGTCG	135	3.8239622E-4	21.089226	8
TATTCCG	205	6.8977533E-7	20.833626	5
ATGGGGG	4175	0.0	20.004715	5
CAGAGTA	6580	0.0	19.651209	10-11
TAAGTAC	1715	0.0	19.092428	1
>>END_MODULE