FastQCFastQC Report
Mon 15 Aug 2016
SRR2921376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972762
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19730.10001206430375281No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22900.060.816291
GTATCAA43950.041.4216771
TATCAAC57450.031.4723032
TCAACGC57350.031.2789334
CAACGCA58150.030.9073835
ATCAACG58550.030.7999693
AACGCAG61800.029.2321056
ACGCAGA72500.024.5893617
GTGGTAT12450.023.6706451
CGCAGAG75000.023.4527858
GTACATG68200.023.3479211
TACATGG70550.022.3996282
TGGTATC11850.021.625642
ACATGGG72350.021.3176043
GCAGAGT81350.020.4533529
CATGGGG61350.020.1892194
TAAGGTG8250.019.0186715
ATGGGGG41750.019.018675
CAGAGTA74600.018.70351410-11
AGAGTAC70900.018.60675810-11
Produced by FastQC (version 0.11.5)