##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921376_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1972762
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43540325695649	33.0	31.0	34.0	29.0	34.0
2	31.566033814519947	34.0	31.0	34.0	29.0	34.0
3	31.631926709861606	34.0	31.0	34.0	28.0	34.0
4	35.14229542134328	37.0	35.0	37.0	33.0	37.0
5	34.89195300801617	37.0	35.0	37.0	32.0	37.0
6	34.849937802938214	37.0	35.0	37.0	32.0	37.0
7	34.72134854584588	37.0	35.0	37.0	32.0	37.0
8	34.67950264654327	37.0	35.0	37.0	32.0	37.0
9	36.29528194480632	39.0	37.0	39.0	32.0	39.0
10-11	36.14894270064002	39.0	37.0	39.0	32.0	39.0
12-13	36.083518691053456	39.0	37.0	39.0	32.0	39.0
14-15	37.298324379727504	40.0	38.0	41.0	31.5	41.0
16-17	37.27434758982584	40.0	38.0	41.0	31.0	41.0
18-19	37.248320628641466	40.0	38.0	41.0	31.0	41.0
20-21	37.21083131163313	40.0	38.0	41.0	31.0	41.0
22-23	37.10424369488058	40.0	37.0	41.0	31.0	41.0
24-25	37.02725341424865	40.0	37.0	41.0	31.0	41.0
26-27	36.88341396478643	40.0	37.0	41.0	30.0	41.0
28-29	36.7190791387912	40.0	37.0	41.0	30.0	41.0
30-31	36.56713937109494	40.0	36.5	41.0	30.0	41.0
32-33	36.49148173981453	40.0	36.0	41.0	30.0	41.0
34-35	36.60753324526729	40.0	37.0	41.0	30.0	41.0
36-37	36.56909272380551	40.0	37.0	41.0	30.0	41.0
38-39	36.467873215319436	40.0	36.0	41.0	30.0	41.0
40-41	36.33354530348821	40.0	36.0	41.0	29.5	41.0
42-43	36.214380143169834	40.0	36.0	41.0	29.0	41.0
44-45	36.04874536310006	40.0	36.0	41.0	28.0	41.0
46-47	35.85843477317589	40.0	35.0	41.0	27.5	41.0
48-49	35.6792182736691	40.0	35.0	41.0	27.0	41.0
50-51	35.516928549921374	39.5	35.0	41.0	26.5	41.0
52-53	35.34399081085301	39.0	35.0	41.0	26.0	41.0
54-55	35.13462216932402	39.0	35.0	41.0	26.0	41.0
56-57	34.861593035551174	39.0	35.0	41.0	25.0	41.0
58-59	34.6344031870038	38.0	34.5	40.0	25.0	41.0
60-61	34.361490387588574	38.0	34.0	40.0	24.0	41.0
62-63	34.06292269417193	37.0	34.0	40.0	23.0	41.0
64-65	33.747685225080374	37.0	34.0	40.0	23.0	41.0
66-67	33.40910713000352	36.0	34.0	39.0	22.0	41.0
68-69	33.03954481077798	36.0	33.0	39.0	22.0	41.0
70-71	32.65532461594455	35.0	33.0	38.5	20.5	40.0
72-73	32.257279134533206	35.0	33.0	37.5	19.5	40.0
74-75	31.84981918751476	35.0	32.5	37.0	18.0	39.0
76-77	29.085116450945428	33.0	28.5	34.5	10.5	36.5
78-79	30.741276190437567	34.5	31.5	36.0	12.0	37.0
80-81	30.942784279097022	35.0	32.0	35.5	12.5	37.0
82-83	30.780440570124526	35.0	32.0	35.0	9.5	37.0
84-85	30.549898061702322	35.0	32.0	35.0	7.0	36.0
86-87	30.305690955117747	35.0	32.0	35.0	2.0	36.0
88-89	30.02967286474496	35.0	31.0	35.0	2.0	36.0
90-91	29.838232386876875	34.0	31.0	35.0	2.0	35.0
92-93	29.686024467219056	34.0	31.0	35.0	2.0	35.0
94-95	29.580934496913464	34.0	31.0	35.0	2.0	35.0
96-97	29.385844820611915	34.0	31.0	35.0	2.0	35.0
98-99	29.1172062316691	34.0	31.0	35.0	2.0	35.0
100-101	28.01120003325287	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32726.0
3	11312.0
4	6443.0
5	4647.0
6	4142.0
7	4303.0
8	4530.0
9	4951.0
10	5471.0
11	6057.0
12	6693.0
13	7324.0
14	8120.0
15	8983.0
16	10325.0
17	11523.0
18	10516.0
19	8661.0
20	8094.0
21	7940.0
22	8235.0
23	9198.0
24	10758.0
25	12834.0
26	15413.0
27	18474.0
28	22067.0
29	26625.0
30	32179.0
31	40341.0
32	50537.0
33	66899.0
34	97787.0
35	142687.0
36	239541.0
37	457112.0
38	485191.0
39	64123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.634354577320345	20.163036193710067	14.240687562702323	24.961921666267266
2	15.53015172682278	21.386730036557992	37.465961325171484	25.61715691144774
3	17.931236556768646	25.07960116533774	29.678092431134413	27.3110698467592
4	13.696653130083506	17.34808349457768	35.02972353839626	33.925539836942555
5	14.391831320707265	37.5116620086238	33.79822494087868	14.298281729790247
6	33.19665337716419	35.196415129249495	17.050389433958436	14.556542059627878
7	28.36572701913714	32.66822488887402	20.823404355621385	18.142643736367457
8	26.81097746121751	34.67772881581035	19.88708051216257	18.62421321080957
9	27.034077095970016	15.593011219802491	19.643626549984237	37.729285134243256
10-11	25.61648996969278	25.67626712472138	27.52283824773774	21.1844046578481
12-13	26.119937316104302	23.19022808452441	27.498465978171144	23.19136862120014
14-15	23.60468756138579	24.90087660490174	25.02454164221796	26.469894191494515
16-17	23.375837344221658	27.807026214608506	25.961079809707744	22.856056631462092
18-19	23.44258609620735	27.085293900720796	27.508763783744467	21.96335621932739
20-21	23.885415296428853	26.047127816797083	27.573810354777855	22.49364653199621
22-23	23.926242290926428	25.709229699661364	27.163134426372437	23.201393583039774
24-25	23.62361568248558	26.041162393301036	27.08826834786232	23.246953576351064
26-27	23.2749548588687	26.338494106053524	27.24423151596136	23.14231951911642
28-29	23.332441122988662	26.78301393723807	26.936337243343782	22.948207696429485
30-31	24.050802332204544	26.190887205549796	26.738754002915638	23.019556459330023
32-33	23.691111387694047	26.22869786809478	26.60763620702723	23.472554537183942
34-35	23.93363205011182	26.384927924459145	26.761293828770246	22.920146196658788
36-37	23.80253453301229	26.38291724749715	26.89646432644785	22.918083893042706
38-39	23.796246521048086	26.37646866199655	26.77167709243386	23.0556077245215
40-41	23.964803824282725	26.184804001708358	26.679111807295484	23.171280366713432
42-43	23.765203346662663	26.251703342607087	26.346198301524087	23.63689500920616
44-45	23.595597039818877	26.247299225076574	26.62920983766454	23.527893897440006
46-47	23.736815703618312	26.17321258696484	26.33972171759149	23.750249991825363
48-49	23.428940642489433	26.45545911710871	26.47036382960872	23.645236410793142
50-51	23.663620429852696	26.337608879414898	26.57345883271382	23.42531185801858
52-53	23.952260934626434	26.149476561343604	26.344511447115764	23.553751056914194
54-55	23.189997052320923	26.041946258969446	26.77306191367989	23.99499477502974
56-57	23.404437061199122	26.213624470219905	26.353969780007397	24.02796868857358
58-59	23.445608069059077	26.225202426071135	26.580905538555754	23.74828396631403
60-61	23.691738665587884	26.10092066355098	26.68697489961214	23.520365771248997
62-63	23.556637721131676	26.120998335769052	26.748310735873996	23.57405320722527
64-65	23.195374170219218	25.874099987982092	26.751305869962177	24.179219971836513
66-67	23.34429769236289	26.127189438312616	26.35624675614118	24.172266113183323
68-69	23.1846084458537	26.135553799478096	26.5366797510494	24.143158003618804
70-71	23.159850386774682	25.933821174617478	26.473406530656334	24.432921907951506
72-73	23.139338256731644	26.204977652030838	26.30562590792586	24.350058183311656
74-75	23.062187856509063	26.237799467613133	26.28097350741539	24.419039168462415
76-77	22.98319160121259	25.83982919477573	26.436544756462048	24.74043444754963
78-79	22.8809881615908	25.889453226553037	26.57901044341722	24.65054816843894
80-81	23.120690398480434	26.068020379232255	26.31380873040629	24.49748049188102
82-83	23.001065996732294	26.084141492370073	26.42077337365784	24.494019137239793
84-85	22.962439173173497	26.13954351578994	26.26671794886097	24.631299362175596
86-87	22.68019819385161	26.324609019174822	26.214822633346195	24.780370153627377
88-89	22.588564880828795	25.990984111268638	26.322999969758985	25.097451038143575
90-91	22.661765958555875	25.74593193338529	26.453296853486037	25.1390052545728
92-93	22.798074228670764	25.677804813413957	26.426000195265196	25.098120762650076
94-95	22.556835204141375	25.717514828834116	26.46898098370894	25.256668983315567
96-97	22.304700134138912	25.55589577518197	26.60814607453581	25.531258016143312
98-99	22.023024268981615	25.552219255965486	26.484169642850354	25.94058683220255
100-101	22.044197634749814	25.579298738296675	26.489940336488015	25.886563290465496
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	140.0
1	109.0
2	89.5
3	107.0
4	106.5
5	107.5
6	171.5
7	397.5
8	587.0
9	611.0
10	539.5
11	424.5
12	422.0
13	485.0
14	505.0
15	545.5
16	611.5
17	683.0
18	799.0
19	896.5
20	1003.0
21	1141.0
22	1318.5
23	1632.0
24	1960.0
25	2503.0
26	3362.0
27	4526.0
28	6346.0
29	8868.5
30	12044.0
31	15942.5
32	21392.0
33	25482.5
34	28395.5
35	32331.0
36	37224.5
37	42592.5
38	47341.5
39	51648.0
40	55364.5
41	58687.0
42	62496.0
43	66506.5
44	70609.5
45	76289.0
46	79965.0
47	82081.5
48	85132.5
49	87170.0
50	87132.5
51	85523.5
52	84008.0
53	82642.0
54	80166.5
55	74891.5
56	68328.5
57	61314.5
58	53881.0
59	46071.0
60	37799.0
61	31244.0
62	25794.5
63	19521.5
64	13752.5
65	10160.5
66	7828.5
67	5937.0
68	4546.0
69	3481.0
70	2556.0
71	1867.5
72	1329.0
73	1022.0
74	805.0
75	567.5
76	384.5
77	231.5
78	121.5
79	82.5
80	51.5
81	31.0
82	23.5
83	11.5
84	8.0
85	5.5
86	3.0
87	3.0
88	3.5
89	3.5
90	1.0
91	0.0
92	1.0
93	1.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15906632427023634
2	3.0414211141536584E-4
3	0.005829390468794512
4	0.004207299207912561
5	0.027676932138798297
6	0.0013686395013691463
7	5.0690351902560976E-5
8	0.0014700202051742685
9	0.0
10-11	0.10693129733845239
12-13	3.294872873666464E-4
14-15	0.004790238254792013
16-17	3.5483246331792685E-4
18-19	6.336293987820122E-4
20-21	0.0010391522140025002
22-23	2.787969354640854E-4
24-25	0.0019769237241998782
26-27	0.0030414211141536586
28-29	0.00674181680304061
30-31	0.014877618283401646
32-33	0.014066572652960671
34-35	0.010822390131196768
36-37	6.082842228307317E-4
38-39	2.787969354640854E-4
40-41	0.005297141773817622
42-43	0.008617359823435367
44-45	0.009124263342460977
46-47	0.010011344500755793
48-49	0.011734816465442867
50-51	0.005550593533330427
52-53	0.0019262333722973172
54-55	0.001191223269710183
56-57	0.006463019867576525
58-59	0.009631166861486585
60-61	0.00826252736011744
62-63	0.019236988547021893
64-65	0.03573669809130549
66-67	0.03885415473331299
68-69	0.028564013297093113
70-71	0.04448078379449726
72-73	0.027727622490700855
74-75	0.02595346017411122
76-77	0.2487122116099154
78-79	0.07220840628519812
80-81	0.05814183363223745
82-83	0.02177150614214994
84-85	0.033835809894959455
86-87	0.08422201968610507
88-89	0.265389337385858
90-91	0.32337909996238773
92-93	0.054973686638327385
94-95	0.004308679911717683
96-97	0.009124263342460977
98-99	8.870811582948171E-4
100-101	0.0016220912608819512
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1972762.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.10318224057077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.15946435545507	50.58317287528817
2	11.37470421321784	14.355600657985468
3	3.417815221799976	6.470250504175221
4	1.6277894659580805	4.1087478127853645
5	0.8941730266182559	2.8212581726647272
6	0.5688918568912054	2.153933191234946
7	0.3712698751083716	1.6399817412578264
8	0.281014768486006	1.4186340918451392
9	0.21156401810052605	1.2015326510770414
>10	1.0395036937245359	11.092858458493575
>50	0.03753015191377032	1.5878687697688774
>100	0.014990598455518738	1.8124863102158915
>500	0.0010471128451759284	0.49613025112426756
>1k	2.4164142580982903E-4	0.25754451208346063
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1973	0.10001206430375281	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	5.0690351902560976E-5
10-11	0.0	0.0	0.0	2.5345175951280488E-5	5.0690351902560976E-5
12-13	0.0	5.0690351902560976E-5	0.0	5.0690351902560976E-5	5.0690351902560976E-5
14-15	0.0	5.0690351902560976E-5	0.0	5.0690351902560976E-5	5.0690351902560976E-5
16-17	0.0	5.0690351902560976E-5	0.0	5.0690351902560976E-5	5.0690351902560976E-5
18-19	0.0	5.0690351902560976E-5	0.0	7.603552785384146E-5	5.0690351902560976E-5
20-21	0.0	5.0690351902560976E-5	0.0	1.774162316589634E-4	5.0690351902560976E-5
22-23	0.0	5.0690351902560976E-5	0.0	2.534517595128049E-4	5.0690351902560976E-5
24-25	0.0	5.0690351902560976E-5	5.0690351902560976E-5	4.562131671230488E-4	1.2672587975640244E-4
26-27	0.0	5.0690351902560976E-5	5.0690351902560976E-5	0.0015714009089793902	1.774162316589634E-4
28-29	0.0	5.0690351902560976E-5	5.0690351902560976E-5	0.004714202726938171	2.027614076102439E-4
30-31	0.0	5.0690351902560976E-5	5.0690351902560976E-5	0.016575745072137438	2.027614076102439E-4
32-33	0.0	5.0690351902560976E-5	5.0690351902560976E-5	0.04516510354518183	2.027614076102439E-4
34-35	0.0	7.603552785384146E-5	5.0690351902560976E-5	0.09045693297012006	2.027614076102439E-4
36-37	0.0	1.2672587975640244E-4	5.0690351902560976E-5	0.1527807206343188	2.534517595128049E-4
38-39	0.0	1.5207105570768292E-4	5.0690351902560976E-5	0.2392838061560391	2.534517595128049E-4
40-41	0.0	1.5207105570768292E-4	5.0690351902560976E-5	0.33480977431641523	2.7879693546408536E-4
42-43	0.0	1.5207105570768292E-4	5.0690351902560976E-5	0.4438700664347752	3.294872873666464E-4
44-45	0.0	1.5207105570768292E-4	5.0690351902560976E-5	0.5851187320112614	3.5483246331792685E-4
46-47	0.0	1.5207105570768292E-4	5.0690351902560976E-5	0.7462887058854539	3.8017763926920733E-4
48-49	0.0	1.5207105570768292E-4	5.0690351902560976E-5	0.9009703147161188	4.055228152204878E-4
50-51	0.0	1.5207105570768292E-4	5.0690351902560976E-5	1.0578316086785937	4.055228152204878E-4
52-53	0.0	1.5207105570768292E-4	5.0690351902560976E-5	1.2214600646200606	4.562131671230488E-4
54-55	0.0	1.5207105570768292E-4	5.0690351902560976E-5	1.4288596394293889	5.069035190256098E-4
56-57	0.0	1.5207105570768292E-4	5.0690351902560976E-5	1.6720466026819252	5.322486949768902E-4
58-59	0.0	2.027614076102439E-4	5.0690351902560976E-5	1.9211136467551584	6.082842228307317E-4
60-61	0.0	2.027614076102439E-4	5.0690351902560976E-5	2.169318954846048	6.082842228307317E-4
62-63	0.0	2.027614076102439E-4	5.0690351902560976E-5	2.431971013229168	7.096649266358537E-4
64-65	0.0	2.027614076102439E-4	5.0690351902560976E-5	2.7339080943367726	7.603552785384147E-4
66-67	0.0	2.027614076102439E-4	5.0690351902560976E-5	3.0999938157770677	8.110456304409756E-4
68-69	0.0	2.027614076102439E-4	5.0690351902560976E-5	3.4901321091951285	8.110456304409756E-4
70-71	0.0	2.027614076102439E-4	5.0690351902560976E-5	3.8905098536975062	9.124263342460976E-4
72-73	0.0	2.027614076102439E-4	5.0690351902560976E-5	4.302774485721034	0.0011151877418563415
74-75	0.0	2.027614076102439E-4	5.0690351902560976E-5	4.752499287800555	0.0011151877418563415
76-77	0.0	2.027614076102439E-4	5.0690351902560976E-5	5.283810211267249	0.0011151877418563415
78-79	0.0	2.027614076102439E-4	5.0690351902560976E-5	5.8707031055951	0.0011151877418563415
80-81	0.0	2.027614076102439E-4	5.0690351902560976E-5	6.464540578133601	0.0011151877418563415
82-83	0.0	2.027614076102439E-4	5.0690351902560976E-5	7.060456355100108	0.0011658780937589025
84-85	0.0	2.027614076102439E-4	5.0690351902560976E-5	7.705415047532343	0.001191223269710183
86-87	0.0	3.0414211141536584E-4	5.0690351902560976E-5	8.42088401946104	0.0012672587975640244
88-89	0.0	3.0414211141536584E-4	5.0690351902560976E-5	9.190591667925478	0.0013179491494665855
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2290	0.0	60.81629	1
GTATCAA	4395	0.0	41.421677	1
TATCAAC	5745	0.0	31.472303	2
TCAACGC	5735	0.0	31.278933	4
CAACGCA	5815	0.0	30.907383	5
ATCAACG	5855	0.0	30.799969	3
AACGCAG	6180	0.0	29.232105	6
ACGCAGA	7250	0.0	24.589361	7
GTGGTAT	1245	0.0	23.670645	1
CGCAGAG	7500	0.0	23.452785	8
GTACATG	6820	0.0	23.347921	1
TACATGG	7055	0.0	22.399628	2
TGGTATC	1185	0.0	21.62564	2
ACATGGG	7235	0.0	21.317604	3
GCAGAGT	8135	0.0	20.453352	9
CATGGGG	6135	0.0	20.189219	4
TAAGGTG	825	0.0	19.018671	5
ATGGGGG	4175	0.0	19.01867	5
CAGAGTA	7460	0.0	18.703514	10-11
AGAGTAC	7090	0.0	18.606758	10-11
>>END_MODULE