FastQCFastQC Report
Mon 15 Aug 2016
SRR2921375_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921375_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3188397
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30350.052.0694271
GTATCAA55300.043.5090981
TCAACGC68000.034.7593964
TATCAAC69450.034.3065032
ATCAACG70250.033.915293
CAACGCA69050.033.7454385
AACGCAG74500.031.2023376
GTACATG122100.030.31631
TACATGG127550.028.57762
ACATGGG130750.027.1517643
ACGCAGA87750.026.116947
CGCAGAG91950.024.924788
CATGGGG110550.024.6007124
ATGGGGG75650.024.1517755
GTGGTAT16000.021.6521051
GCAGAGT100950.021.2452189
GAGTACA91650.020.660351
GTATTAC5750.020.6333351
AGTACAT94700.018.894542
GTAAGTA20800.018.4827522
Produced by FastQC (version 0.11.5)