##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921375_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3188397
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.386519307350998	31.0	31.0	34.0	26.0	34.0
2	30.422498829349042	31.0	31.0	34.0	26.0	34.0
3	30.47024037470867	33.0	31.0	34.0	26.0	34.0
4	33.939351341755746	37.0	35.0	37.0	30.0	37.0
5	33.938772367431035	37.0	35.0	37.0	30.0	37.0
6	33.978852382560895	37.0	35.0	37.0	30.0	37.0
7	33.898905939254114	37.0	35.0	37.0	30.0	37.0
8	33.86428697555543	37.0	35.0	37.0	30.0	37.0
9	35.417216237501165	39.0	37.0	39.0	30.0	39.0
10-11	35.30477587954073	39.0	35.5	39.0	29.0	39.0
12-13	35.18118022943818	39.0	35.0	39.0	28.5	39.0
14-15	36.297848730882635	40.0	36.5	41.0	28.0	41.0
16-17	36.23616757888055	40.0	36.0	41.0	27.0	41.0
18-19	36.18307303011514	40.0	36.5	41.0	27.0	41.0
20-21	36.12091389497606	40.0	36.0	41.0	27.0	41.0
22-23	36.00791573320386	40.0	36.0	41.0	27.0	41.0
24-25	35.9029520163267	40.0	36.0	41.0	27.0	41.0
26-27	35.74850010836166	40.0	36.0	41.0	27.0	41.0
28-29	35.60507835128436	40.0	36.0	41.0	26.0	41.0
30-31	35.45747204629787	40.0	35.5	41.0	25.0	41.0
32-33	35.277306903751324	39.0	35.0	41.0	25.0	41.0
34-35	35.122772979650904	39.0	35.0	41.0	24.5	41.0
36-37	34.957959752188955	39.0	35.0	41.0	24.0	41.0
38-39	35.196842645379476	39.0	35.0	41.0	24.5	41.0
40-41	35.32244980785015	40.0	35.0	41.0	24.5	41.0
42-43	35.16134894744914	40.0	35.0	41.0	24.0	41.0
44-45	35.046430855379676	40.0	35.0	41.0	23.0	41.0
46-47	34.897569687840004	39.5	35.0	41.0	23.0	41.0
48-49	34.755135417578174	39.0	35.0	41.0	22.0	41.0
50-51	33.04043630702199	37.0	32.5	39.5	18.5	40.0
52-53	33.193561059052556	37.5	33.0	39.5	18.5	40.0
54-55	34.04324147839808	38.5	34.0	40.5	19.5	41.0
56-57	34.05643980344982	38.5	34.0	41.0	19.0	41.0
58-59	33.871015905484796	38.0	34.0	40.5	18.0	41.0
60-61	33.61774929533556	37.5	34.0	40.0	17.0	41.0
62-63	33.31629781360351	37.0	33.5	40.0	13.5	41.0
64-65	33.02416355303308	36.5	33.0	40.0	12.5	41.0
66-67	32.683632402113034	36.0	33.0	39.0	8.0	41.0
68-69	32.312191204545734	35.5	33.0	39.0	7.0	41.0
70-71	31.932299992754977	35.0	32.0	38.5	4.0	40.5
72-73	31.577919562714428	35.0	32.0	37.0	2.0	39.5
74-75	31.179926150978062	35.0	32.0	37.0	2.0	39.0
76-77	30.810567661429864	35.0	32.0	36.0	2.0	39.0
78-79	30.476351596115542	35.0	31.0	36.0	2.0	37.5
80-81	30.135813545176465	35.0	31.0	35.5	2.0	37.0
82-83	29.83981809667993	35.0	31.0	35.0	2.0	37.0
84-85	29.614396199720424	35.0	31.0	35.0	2.0	36.0
86-87	29.394910671412624	35.0	31.0	35.0	2.0	36.0
88-89	29.198278790251027	35.0	31.0	35.0	2.0	36.0
90-91	28.940868405032372	34.5	30.0	35.0	2.0	35.0
92-93	28.740938001133486	34.0	30.0	35.0	2.0	35.0
94-95	28.52194315827044	34.0	30.0	35.0	2.0	35.0
96-97	28.25739486017582	34.0	29.5	35.0	2.0	35.0
98-99	28.022523543962684	34.0	29.0	35.0	2.0	35.0
100-101	26.975135467760133	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	111949.0
3	19361.0
4	13387.0
5	10070.0
6	9112.0
7	9359.0
8	9717.0
9	10414.0
10	11292.0
11	11854.0
12	12155.0
13	12004.0
14	12663.0
15	13695.0
16	13716.0
17	14134.0
18	13311.0
19	12884.0
20	13064.0
21	13985.0
22	15507.0
23	17539.0
24	20510.0
25	24344.0
26	29091.0
27	34314.0
28	41138.0
29	48478.0
30	57995.0
31	71237.0
32	88500.0
33	114943.0
34	165132.0
35	234175.0
36	382203.0
37	686271.0
38	696015.0
39	112874.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.48119804342669	17.101063262286008	12.00390611761782	25.41383257666948
2	16.70034454049708	19.6397497868393	38.479515390092715	25.180390282570908
3	19.68501565037302	23.916845135832688	28.77537988436767	27.62275932942662
4	14.001000421130852	15.372531347888604	35.36181165346265	35.26465657751789
5	14.68721847403395	37.38291422344097	33.42571228913107	14.504155013394007
6	34.034843039438314	33.75857438220688	17.373901254109757	14.832681324245044
7	29.468402793026215	31.620701769794913	20.66353193598374	18.247363501195128
8	27.69824581436356	33.70647043193829	19.875228492098024	18.720055261600123
9	28.4448903189498	14.946374284250643	18.589265194841616	38.01947020195794
10-11	26.480437218262644	24.618733547103027	27.208203661670012	21.692625572964317
12-13	27.023585418417294	22.178519087516193	27.016683547492637	23.781211946573872
14-15	24.41858239501474	23.591282697337146	24.256219416723276	27.733915490924836
16-17	24.549556120894742	26.372649505077227	25.114063925995154	23.963730448032873
18-19	24.546062001593647	26.12540233274565	26.17611351273348	23.152422152927226
20-21	25.009901831866344	25.222102990659103	26.209117591096458	23.55887758637809
22-23	24.99415580849879	24.915208233642147	25.86730155243691	24.223334405422154
24-25	24.686043921219174	25.15529938156363	25.769013545356707	24.389643151860486
26-27	24.337145470103838	25.484880737715525	25.88650807905798	24.291465713122655
28-29	24.35886375892129	25.961065193856836	25.51391440767265	24.16615663954922
30-31	25.04772992508288	25.39206920223545	25.460105812770085	24.100095059911585
32-33	24.659651073048945	25.420075383729152	25.473524339114174	24.44674920410773
34-35	24.863550939283638	25.62395813549442	25.588952899608213	23.923538025613734
36-37	24.806247988774142	25.603618669696026	25.746529400436714	23.843603941093118
38-39	24.76883794873291	25.615244156903668	25.614538426324312	24.001379468039108
40-41	24.814782520601238	25.37560232338512	25.58383378618998	24.225781369823657
42-43	24.507452624035565	25.4383686182806	25.389100915499213	24.665077842184626
44-45	24.28620762361021	25.481342243581345	25.722857853012332	24.509592279796117
46-47	24.39884356734335	25.36633461127522	25.499308654390045	24.735513166991392
48-49	24.265720806181086	25.562005318501235	25.642324899934405	24.529948975383274
50-51	24.395327246265737	25.41156681107387	25.927511072501968	24.265594870158427
52-53	24.633334045631496	25.195085896873472	25.779447977303665	24.392132080191363
54-55	23.84057283316228	25.162685271719287	26.19671248167159	24.800029413446847
56-57	24.090419961859364	25.318708146181475	25.751310220157215	24.83956167180195
58-59	24.294163141895215	25.37543176420638	25.866223256232	24.464181837666402
60-61	24.63243260996065	25.228504400688724	26.059943777663904	24.079119211686727
62-63	24.418389044663975	25.17003788257345	26.117887903535518	24.293685169227057
64-65	24.04254503364186	24.970654316808925	26.183200660988927	24.80359998856029
66-67	24.19714271124257	25.18485173444577	25.867662828927983	24.750342725383675
68-69	24.064837670142715	25.272314757931092	26.000147694009435	24.66269987791676
70-71	24.038170608367633	25.05505436628489	25.991442026943258	24.915332998404217
72-73	23.98169500323486	25.527498814618234	25.755510022092565	24.735296160054347
74-75	23.96950481777815	25.439103507250742	25.93366201256828	24.657729662402822
76-77	23.948177305694355	24.96123823969999	25.9315822920537	25.159002162551953
78-79	23.86036487935623	25.14094382864348	26.104607381314267	24.89408391068602
80-81	24.200769358551984	25.33178332170091	25.71949577578873	24.747951543958376
82-83	24.145919441781523	25.407631500369316	25.891562288821486	24.554886769027675
84-85	24.0702169260734	25.491807323356852	25.70626929206964	24.731706458500106
86-87	23.79839882911078	25.722988257158054	25.68913281017185	24.789480103559317
88-89	23.745210580432545	25.36342063811147	25.91169896181698	24.979669819639003
90-91	24.0655102732691	25.082833227216483	26.035653061862796	24.81600343765162
92-93	24.286587742681824	24.96859596620058	25.959237626999297	24.785578664118297
94-95	24.191611589377686	25.00517565391263	25.932501927606864	24.870710829102816
96-97	23.937800784249614	24.90233820823564	26.070774244104573	25.089086763410172
98-99	23.672118933982585	24.884972694129452	26.007605877540502	25.43530249434746
100-101	24.026010058257917	24.96842352141518	26.024268655745097	24.981297764581807
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	132.0
1	103.0
2	118.0
3	153.0
4	138.5
5	168.0
6	260.5
7	486.5
8	696.5
9	785.5
10	738.0
11	638.5
12	623.0
13	686.5
14	779.5
15	837.5
16	933.5
17	1021.0
18	1136.0
19	1251.5
20	1348.5
21	1446.5
22	1535.0
23	1681.5
24	1886.5
25	2116.5
26	2502.5
27	3172.5
28	4178.0
29	5876.0
30	8689.5
31	13072.5
32	19598.0
33	25263.0
34	30355.5
35	37326.0
36	45905.5
37	54560.5
38	62609.0
39	70763.5
40	78260.0
41	85374.5
42	91956.5
43	99488.0
44	107729.0
45	116293.5
46	124364.5
47	132047.0
48	140286.0
49	146428.5
50	148962.0
51	149528.0
52	148892.5
53	148076.5
54	145364.0
55	137947.5
56	128057.0
57	115770.0
58	103268.0
59	90021.5
60	75094.0
61	62018.0
62	50793.0
63	39600.5
64	29263.0
65	21753.0
66	16487.0
67	12482.5
68	9437.0
69	7192.0
70	5465.0
71	4072.5
72	2968.5
73	2172.0
74	1614.5
75	1185.0
76	855.0
77	567.5
78	362.5
79	256.5
80	193.5
81	156.5
82	120.0
83	95.0
84	84.0
85	70.0
86	56.5
87	55.5
88	56.5
89	47.5
90	34.5
91	29.5
92	32.5
93	26.0
94	17.5
95	14.5
96	15.0
97	10.0
98	4.5
99	7.5
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05021332036129754
2	0.05752106779676433
3	0.04930377239722657
4	0.054541514121359415
5	0.04779831369807461
6	0.017500957377641492
7	0.014333221364842583
8	0.02038641988434941
9	0.020511874775945405
10-11	0.021907560444950864
12-13	0.02683166494009372
14-15	0.04397193950439673
16-17	0.029168262296069152
18-19	0.023115063776562327
20-21	0.02683166494009372
22-23	0.04616740010732666
24-25	0.041619660286971796
26-27	0.031034403808559596
28-29	0.0206373296675414
30-31	0.012780717081342128
32-33	0.009675708514341219
34-35	0.009487526176947224
36-37	0.0013486400846569608
38-39	0.006633427393138307
40-41	0.0037009193020818925
42-43	0.2132576338517443
44-45	0.18799415505660053
46-47	0.19436099080509736
48-49	0.19161666505143493
50-51	0.19058166219576797
52-53	0.19473735547988535
54-55	0.19379644379291538
56-57	0.2035191978916051
58-59	0.20053964421620019
60-61	0.20591852269337854
62-63	0.20257828620463514
64-65	0.20380147139769608
66-67	0.19396894426885988
68-69	0.1926359860456524
70-71	0.17670321481296086
72-73	0.18435596320031664
74-75	0.18383846177248314
76-77	0.1823643667962302
78-79	0.20803557398906097
80-81	0.21689582570802818
82-83	0.21474741068944675
84-85	0.2105760355438799
86-87	0.22660289794526842
88-89	0.22475243829422745
90-91	0.22442311920378796
92-93	0.22823381153601638
94-95	0.23960316108690352
96-97	0.23991679831589355
98-99	0.22909631391573884
100-101	0.23922679641211558
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3188397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.06431882576188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.97460751654943	42.59464618469952
2	12.794717839470293	14.346542804754517
3	4.472553158611766	7.522519387487353
4	2.0567248933528757	4.61235520631267
5	1.2044594214404551	3.376359850816519
6	0.7607691652526385	2.5591203020119595
7	0.5079969952860932	1.993635385437401
8	0.3851433193567216	1.7274238280021974
9	0.2741004725673569	1.383053065587747
>10	1.482487054293087	14.625873623629419
>50	0.06448881179248123	2.4265328194281217
>100	0.020213268364475498	1.9314248439084236
>500	0.0011213442992294037	0.4400403961732614
>1k	6.16739363123741E-4	0.460472301750833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.136372289899909E-5	0.0	0.0	0.0
3	0.0	6.272744579799818E-5	0.0	0.0	0.0
4	0.0	6.272744579799818E-5	0.0	3.136372289899909E-5	6.272744579799818E-5
5	0.0	6.272744579799818E-5	0.0	3.136372289899909E-5	6.272744579799818E-5
6	0.0	6.272744579799818E-5	0.0	3.136372289899909E-5	9.409116869699727E-5
7	0.0	6.272744579799818E-5	0.0	3.136372289899909E-5	9.409116869699727E-5
8	0.0	6.272744579799818E-5	0.0	3.136372289899909E-5	1.2545489159599636E-4
9	0.0	6.272744579799818E-5	0.0	3.136372289899909E-5	1.8818233739399453E-4
10-11	0.0	6.272744579799818E-5	0.0	6.272744579799818E-5	2.1954606029299362E-4
12-13	0.0	7.840930724749772E-5	0.0	6.272744579799818E-5	2.822735060909918E-4
14-15	0.0	1.0977303014649681E-4	0.0	6.272744579799818E-5	3.920465362374886E-4
16-17	0.0	1.2545489159599636E-4	0.0	6.272744579799818E-5	4.3909212058598724E-4
18-19	0.0	1.2545489159599636E-4	0.0	9.409116869699727E-5	4.3909212058598724E-4
20-21	0.0	1.2545489159599636E-4	0.0	1.0977303014649681E-4	4.861377049344859E-4
22-23	0.0	1.2545489159599636E-4	0.0	1.8818233739399453E-4	5.331832892829845E-4
24-25	0.0	1.2545489159599636E-4	0.0	3.606828133384895E-4	5.645470121819836E-4
26-27	0.0	1.2545489159599636E-4	0.0	0.0011918214701619654	5.645470121819836E-4
28-29	0.0	1.2545489159599636E-4	0.0	0.003998874669622384	5.645470121819836E-4
30-31	0.0	1.2545489159599636E-4	0.0	0.015352542359060056	6.115925965304822E-4
32-33	0.0	1.2545489159599636E-4	0.0	0.04375239344410373	8.154567953739763E-4
34-35	0.0	1.8818233739399453E-4	0.0	0.08192204421218563	8.468205182729754E-4
36-37	0.0	2.0386419884349408E-4	0.0	0.13713787837587352	8.468205182729754E-4
38-39	0.0	2.509097831919927E-4	0.0	0.22261970513709556	8.93866102621474E-4
40-41	0.0	2.822735060909918E-4	0.0	0.32550839810726206	9.565935484194722E-4
42-43	0.0	3.136372289899909E-4	0.0	0.4345286989041829	9.722754098689718E-4
44-45	0.0	3.136372289899909E-4	0.0	0.5747872677085069	0.0010036391327679708
46-47	0.0	3.4500095188899E-4	0.0	0.7294417853234714	0.0010506847171164696
48-49	0.0	3.606828133384895E-4	0.0	0.8842217578300318	0.0010977303014649681
50-51	0.0	3.7636467478798907E-4	0.0	1.047564026688019	0.0010977303014649681
52-53	0.0	3.7636467478798907E-4	0.0	1.2219620078679037	0.0011447758858134666
54-55	0.0	3.7636467478798907E-4	0.0	1.4296055353207269	0.0011918214701619654
56-57	0.0	3.7636467478798907E-4	0.0	1.6665741436841146	0.0011918214701619654
58-59	0.0	3.7636467478798907E-4	0.0	1.9161666505143495	0.0011918214701619654
60-61	0.0	4.0772839768698815E-4	0.0	2.1821310206978612	0.0011918214701619654
62-63	0.0	4.0772839768698815E-4	0.0	2.459025648311675	0.0012545489159599636
64-65	0.0	4.0772839768698815E-4	0.0	2.769212867782776	0.0012545489159599636
66-67	0.0	4.3909212058598724E-4	0.0	3.132530233844782	0.0013015945003084623
68-69	0.0	4.3909212058598724E-4	0.0	3.521863808051507	0.0013643219461064605
70-71	0.0	4.7045584348498633E-4	0.0	3.9220805941041847	0.0013956856690054593
72-73	0.0	5.018195663839854E-4	0.0	4.341539024155399	0.0015054586991519563
74-75	0.0	5.018195663839854E-4	0.0	4.788989576893969	0.0015054586991519563
76-77	0.0	5.331832892829845E-4	0.0	5.299794849888517	0.0015211405606014557
78-79	0.0	5.331832892829845E-4	0.0	5.859260938960864	0.0015368224220509554
80-81	0.0	5.331832892829845E-4	0.0	6.426348412697666	0.0015995498678489535
82-83	0.0	5.331832892829845E-4	0.0	7.012363893204014	0.0017250047594449499
84-85	0.0	5.331832892829845E-4	0.0	7.630574235266185	0.0017250047594449499
86-87	0.0	5.331832892829845E-4	0.0	8.306086099064828	0.0017406866208944495
88-89	0.0	5.331832892829845E-4	0.0	9.040357897714745	0.0017720503437934484
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3035	0.0	52.069427	1
GTATCAA	5530	0.0	43.509098	1
TCAACGC	6800	0.0	34.759396	4
TATCAAC	6945	0.0	34.306503	2
ATCAACG	7025	0.0	33.91529	3
CAACGCA	6905	0.0	33.745438	5
AACGCAG	7450	0.0	31.202337	6
GTACATG	12210	0.0	30.3163	1
TACATGG	12755	0.0	28.5776	2
ACATGGG	13075	0.0	27.151764	3
ACGCAGA	8775	0.0	26.11694	7
CGCAGAG	9195	0.0	24.92478	8
CATGGGG	11055	0.0	24.600712	4
ATGGGGG	7565	0.0	24.151775	5
GTGGTAT	1600	0.0	21.652105	1
GCAGAGT	10095	0.0	21.245218	9
GAGTACA	9165	0.0	20.66035	1
GTATTAC	575	0.0	20.633335	1
AGTACAT	9470	0.0	18.89454	2
GTAAGTA	2080	0.0	18.482752	2
>>END_MODULE