FastQCFastQC Report
Mon 15 Aug 2016
SRR2921375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3188397
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28850.053.536751
GTATCAA54200.044.805991
TCAACGC72750.033.2036364
CAACGCA73500.032.8616035
TATCAAC74200.032.810612
ATCAACG74900.032.313893
AACGCAG77950.030.860226
GTACATG125200.030.3668751
TACATGG131200.028.611692
ACATGGG136950.027.0638683
ACGCAGA95450.024.90347
CATGGGG110750.024.896214
CGCAGAG99150.023.5425428
ATGGGGG74850.023.2488575
GCAGAGT111050.019.6070169
TGGGGGG71950.018.2365576
AGAGTAC96400.017.87703710-11
GTATACG1901.859741E-417.5089021
GTGGTAT15150.017.2529961
TAAGTAC31000.017.1700211
Produced by FastQC (version 0.11.5)