##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921375_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3188397
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38172128502191	33.0	31.0	34.0	29.0	34.0
2	31.503894590290983	34.0	31.0	34.0	28.0	34.0
3	31.57637301753828	34.0	31.0	34.0	28.0	34.0
4	35.06911999979927	37.0	35.0	37.0	32.0	37.0
5	34.808926868266404	37.0	35.0	37.0	32.0	37.0
6	34.755296156658034	37.0	35.0	37.0	32.0	37.0
7	34.623836053038566	37.0	35.0	37.0	32.0	37.0
8	34.585035991440215	37.0	35.0	37.0	32.0	37.0
9	36.19882342129917	39.0	37.0	39.0	32.0	39.0
10-11	36.04795968005239	39.0	37.0	39.0	31.5	39.0
12-13	35.967463430683196	39.0	37.0	39.0	31.0	39.0
14-15	37.191691153893316	40.0	38.0	41.0	31.0	41.0
16-17	37.16224830220327	40.0	38.0	41.0	31.0	41.0
18-19	37.12376250510837	40.0	38.0	41.0	31.0	41.0
20-21	37.08276541472094	40.0	38.0	41.0	31.0	41.0
22-23	36.975109435870124	40.0	37.0	41.0	31.0	41.0
24-25	36.90048823907437	40.0	37.0	41.0	30.0	41.0
26-27	36.757825170454	40.0	37.0	41.0	30.0	41.0
28-29	36.60406122575074	40.0	37.0	41.0	30.0	41.0
30-31	36.46413166239964	40.0	36.5	41.0	30.0	41.0
32-33	36.41785621426692	40.0	36.5	41.0	30.0	41.0
34-35	36.55242430600706	40.0	37.0	41.0	30.0	41.0
36-37	36.53103926518561	40.0	37.0	41.0	30.0	41.0
38-39	36.44922464172435	40.0	37.0	41.0	30.0	41.0
40-41	36.32969592557012	40.0	36.0	41.0	29.5	41.0
42-43	36.22860986257358	40.0	36.0	41.0	29.0	41.0
44-45	36.09829892576113	40.0	36.0	41.0	28.5	41.0
46-47	35.94601252604365	40.0	35.5	41.0	28.0	41.0
48-49	35.79330397061595	40.0	35.0	41.0	27.5	41.0
50-51	35.63157991304094	39.5	35.0	41.0	27.0	41.0
52-53	35.455665809496125	39.0	35.0	41.0	27.0	41.0
54-55	35.24343471029486	39.0	35.0	41.0	26.0	41.0
56-57	34.97787869578349	39.0	35.0	41.0	26.0	41.0
58-59	34.745919971697376	38.0	35.0	40.0	26.0	41.0
60-61	34.45954424746981	38.0	34.0	40.0	25.5	41.0
62-63	34.14871156258145	37.0	34.0	40.0	25.0	41.0
64-65	33.83007574025443	37.0	34.0	40.0	24.0	41.0
66-67	33.49105302758721	36.0	34.0	39.0	23.0	41.0
68-69	33.12662256299953	36.0	33.5	39.0	22.5	40.5
70-71	32.746962031390694	35.0	33.0	38.5	22.0	40.0
72-73	32.35257231141542	35.0	33.0	37.0	21.5	39.5
74-75	31.949084916338837	35.0	33.0	37.0	20.0	39.0
76-77	29.217509300127933	33.0	28.5	34.5	13.0	36.5
78-79	30.87856813314026	34.5	31.5	35.5	17.5	37.0
80-81	31.08226829971299	35.0	32.0	35.0	18.0	37.0
82-83	30.93125228759154	35.0	32.0	35.0	17.5	37.0
84-85	30.717892094365915	35.0	32.0	35.0	15.0	36.0
86-87	30.48804273746337	35.0	32.0	35.0	10.5	36.0
88-89	30.221082882715045	35.0	32.0	35.0	6.0	35.5
90-91	30.046017795149098	34.5	31.0	35.0	2.0	35.0
92-93	29.907889450404074	34.0	31.0	35.0	2.0	35.0
94-95	29.81609786987003	34.0	31.0	35.0	2.0	35.0
96-97	29.632699754767053	34.0	31.0	35.0	2.0	35.0
98-99	29.37638568848233	34.0	31.0	35.0	2.0	35.0
100-101	28.294056072691074	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62248.0
3	20857.0
4	11619.0
5	8104.0
6	7152.0
7	7042.0
8	7346.0
9	7968.0
10	8569.0
11	9275.0
12	10058.0
13	10366.0
14	11198.0
15	11977.0
16	12862.0
17	13652.0
18	13117.0
19	12198.0
20	11480.0
21	11348.0
22	11993.0
23	13640.0
24	16162.0
25	19576.0
26	23517.0
27	28482.0
28	33957.0
29	41004.0
30	50435.0
31	62424.0
32	80309.0
33	105886.0
34	157826.0
35	232647.0
36	397968.0
37	773152.0
38	781351.0
39	89631.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.120563626614796	18.609045051870574	12.681473188974609	26.58891813254002
2	15.552414293626931	20.926732208709986	37.21622789712413	26.304625600538955
3	18.199151937655277	25.062378281975867	28.665780693800187	28.072689086568666
4	13.554697546624178	16.458193497393562	34.47585830515704	35.51125065082522
5	14.373623546684906	37.792529662372864	33.48588566731712	14.347961123625117
6	34.07602329093438	34.40446749352725	17.115794458915463	14.40371475662291
7	29.30806500580857	31.75948879560606	20.667251705561927	18.265194493023444
8	27.540216055936302	34.39992773671323	19.582315693333932	18.47754051401654
9	28.088817045054306	14.977839961585712	18.52796248396922	38.40538050939077
10-11	26.367373695307133	24.901524542769813	27.24328841861437	21.487813343308684
12-13	26.90511329162885	22.315770061126532	27.133441946263414	23.6456747009812
14-15	24.216930673426994	23.751779016892723	24.275364762174355	27.755925547505928
16-17	24.409600698533712	26.65682994536746	25.06590310542019	23.867666250678635
18-19	24.467207253320485	26.294259042500475	26.213120862329177	23.025412841849864
20-21	24.856305976843355	25.381448588809587	26.207669619479784	23.554575814867274
22-23	24.783679906008945	25.0492999833615	25.90148597010684	24.265534140522714
24-25	24.540497851234278	25.335566259830344	25.76693466384723	24.357001225088148
26-27	24.15114040147326	25.678094988970635	25.921296161254233	24.249468448301872
28-29	24.25942593365411	26.16130711340353	25.507753548217316	24.07151340472504
30-31	24.88705397396701	25.510345079615483	25.490990745836662	24.11161020058085
32-33	24.505811500538126	25.598128299386776	25.446210251692097	24.449849948382997
34-35	24.763230411400198	25.756528749080545	25.607548897981975	23.872691941537287
36-37	24.629935835471837	25.771900089262022	25.83534951758989	23.76281455767625
38-39	24.70398484502771	25.79037869396569	25.57745007868385	23.928186382322753
40-41	24.72269122112468	25.527117999217747	25.582666009667427	24.16752476999015
42-43	24.409386580078703	25.545351720728732	25.38155643785722	24.66370526133534
44-45	24.207093348681948	25.613467466404654	25.6523147207883	24.5271244641251
46-47	24.350559781811736	25.495154203676357	25.48593233058533	24.668353683926572
48-49	24.162080216711722	25.726486772015427	25.648005309888855	24.463427701383996
50-51	24.379309928422117	25.507891508248136	25.83649328752491	24.276305275804837
52-53	24.54488913366939	25.30395089112091	25.690844526186634	24.460315449023067
54-55	23.75906499952013	25.30294552500626	26.119587424514528	24.818402050959083
56-57	24.06897164975252	25.433750647111065	25.62181864830986	24.87545905482656
58-59	24.214363302335602	25.495023184779065	25.755634342016815	24.534979170868514
60-61	24.518182713546278	25.410066941895238	25.924050339100752	24.147700005457732
62-63	24.267970380966272	25.374637486805224	25.95367046080152	24.403721671426982
64-65	23.908398670971266	25.122627686843686	25.988708275682477	24.98026536650258
66-67	24.03999606552871	25.487859536237533	25.633347122808058	24.838797275425698
68-69	23.861437281463424	25.46982569779157	25.75849672090235	24.910240299842652
70-71	23.848011703475354	25.257307808461487	25.75221107045225	25.142469417610908
72-73	23.824379176489355	25.713253128842574	25.506182549319455	24.956185145348613
74-75	23.74436257224817	25.645339600906507	25.60386670447957	25.00643112236575
76-77	23.63349242373988	25.16600430521842	25.702411758584574	25.498091512457123
78-79	23.545768185694996	25.288726277524038	25.872981623050084	25.292523913730886
80-81	23.887385758989595	25.549160192852504	25.454092718324127	25.109361329833767
82-83	23.75343680598243	25.573946010873865	25.592736785325126	25.079880397818577
84-85	23.66045727283423	25.635781795364526	25.434487626965762	25.269273304835483
86-87	23.37144823005544	25.895377810199367	25.349377834055183	25.38379612569001
88-89	23.184552499492536	25.582843336182883	25.571742831172795	25.660861333151786
90-91	23.43798669365775	25.33446768023751	25.68646026503193	25.541085361072813
92-93	23.597486056878928	25.20471641259934	25.615945718359228	25.581851812162505
94-95	23.430520472092592	25.30113490853312	25.55886407836573	25.70948054100856
96-97	23.0511455491365	25.202143736759265	25.709236097736632	26.037474616367607
98-99	22.746633650712003	25.17799525304173	25.6323200895759	26.443051006670366
100-101	22.810658533739645	25.402950611537733	25.598945036250658	26.187445818471964
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	127.0
1	107.0
2	106.0
3	131.0
4	123.0
5	146.5
6	228.5
7	432.5
8	618.0
9	630.0
10	541.5
11	442.0
12	445.0
13	496.5
14	535.5
15	562.0
16	585.5
17	643.5
18	744.5
19	840.0
20	879.0
21	934.5
22	1090.5
23	1305.0
24	1545.5
25	1852.5
26	2316.5
27	3100.0
28	4297.0
29	6197.5
30	9339.0
31	13924.5
32	20589.5
33	26199.0
34	31043.0
35	38015.5
36	46443.5
37	54861.0
38	62745.0
39	71046.0
40	78699.5
41	85428.0
42	91909.5
43	99591.5
44	108123.5
45	117349.5
46	125639.0
47	133355.0
48	141414.5
49	147067.0
50	149985.5
51	150860.5
52	150100.0
53	148860.0
54	146029.5
55	138635.0
56	128141.0
57	115616.0
58	102928.0
59	89073.0
60	73868.5
61	61344.5
62	50367.5
63	38781.0
64	28165.5
65	20677.0
66	15581.0
67	11856.5
68	8988.0
69	6766.0
70	4982.0
71	3607.0
72	2578.0
73	1852.5
74	1372.0
75	953.5
76	658.0
77	411.0
78	229.5
79	135.5
80	76.0
81	52.5
82	38.5
83	22.5
84	17.0
85	14.0
86	10.5
87	9.0
88	7.5
89	6.5
90	3.5
91	3.5
92	2.5
93	2.5
94	2.5
95	0.5
96	0.0
97	1.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15054586991519564
2	3.4500095188899E-4
3	0.005896379905011829
4	0.004296830037162875
5	0.026878710524442222
6	0.0010036391327679708
7	1.5681861449499544E-4
8	0.001756368482343949
9	0.0
10-11	0.10075595981303458
12-13	3.2931909043949043E-4
14-15	0.005943425489360328
16-17	1.2545489159599636E-4
18-19	2.822735060909918E-4
20-21	7.840930724749772E-4
22-23	2.6659164464149226E-4
24-25	0.0017250047594449499
26-27	0.002822735060909918
28-29	0.006633427393138307
30-31	0.015070268852969063
32-33	0.015493679112105552
34-35	0.011980942147417652
36-37	9.722754098689719E-4
38-39	1.411367530454959E-4
40-41	0.005143650555435851
42-43	0.008499568905628753
44-45	0.008452523321280256
46-47	0.010177528080725206
48-49	0.012012305870316651
50-51	0.006006152935158326
52-53	0.001662277313646952
54-55	0.0010977303014649681
56-57	0.005912061766461329
58-59	0.009848208990285713
60-61	0.008123204230840765
62-63	0.01825368672721747
64-65	0.034719641249191996
66-67	0.03711896605096542
68-69	0.026925756108790715
70-71	0.041070795136239305
72-73	0.02623575420501274
74-75	0.024244157800926296
76-77	0.23855247636978705
78-79	0.06914132713084349
80-81	0.05372605732598544
82-83	0.020778466420586898
84-85	0.03205372480277707
86-87	0.08126340603130663
88-89	0.2622477690199809
90-91	0.31561314353262787
92-93	0.05051127572883804
94-95	0.005222059862683349
96-97	0.01000502760478071
98-99	0.001238867054510464
100-101	0.0012859126388589626
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3188397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.090432613940365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.07037376686122	41.356593673065845
2	13.18791338190202	14.530557069683109
3	4.700334511322633	7.768303849769935
4	2.1592405470501133	4.758139834182079
5	1.230193800302356	3.3885954328822083
6	0.7880655089691548	2.60489218903415
7	0.5464856629500026	2.107429211246215
8	0.37687265432923367	1.660966205388919
9	0.29131209907158906	1.4443658607176009
>10	1.5568533108665725	14.991227150456602
>50	0.07054883205654157	2.5967547603514496
>100	0.01986701798612419	1.8630397034419772
>500	0.0013116131079638899	0.4882082447185937
>1k	6.272932245641423E-4	0.4409268150614068
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.136372289899909E-5
5	0.0	0.0	0.0	0.0	3.136372289899909E-5
6	0.0	0.0	0.0	0.0	3.136372289899909E-5
7	0.0	0.0	0.0	0.0	3.136372289899909E-5
8	0.0	0.0	0.0	0.0	6.272744579799818E-5
9	0.0	0.0	0.0	6.272744579799818E-5	6.272744579799818E-5
10-11	0.0	0.0	0.0	6.272744579799818E-5	6.272744579799818E-5
12-13	0.0	0.0	0.0	6.272744579799818E-5	1.0977303014649681E-4
14-15	0.0	4.704558434849863E-5	0.0	6.272744579799818E-5	2.3522792174249317E-4
16-17	0.0	6.272744579799818E-5	0.0	6.272744579799818E-5	3.606828133384895E-4
18-19	0.0	7.840930724749772E-5	0.0	6.272744579799818E-5	3.7636467478798907E-4
20-21	0.0	9.409116869699727E-5	0.0	7.840930724749772E-5	4.3909212058598724E-4
22-23	0.0	9.409116869699727E-5	0.0	1.5681861449499544E-4	4.861377049344859E-4
24-25	0.0	1.0977303014649681E-4	0.0	3.136372289899909E-4	5.645470121819836E-4
26-27	0.0	1.2545489159599636E-4	0.0	0.0010506847171164696	6.586381808789809E-4
28-29	0.0	1.2545489159599636E-4	0.0	0.0038734197780263876	6.586381808789809E-4
30-31	0.0	1.2545489159599636E-4	0.0	0.015446633527757051	6.743200423284804E-4
32-33	0.0	1.2545489159599636E-4	0.0	0.044520804655129205	7.527293495759781E-4
34-35	0.0	1.5681861449499544E-4	0.0	0.0830668200979991	7.527293495759781E-4
36-37	0.0	1.8818233739399453E-4	0.0	0.138533564044879	7.527293495759781E-4
38-39	0.0	2.1954606029299362E-4	0.0	0.22434470989654048	7.840930724749772E-4
40-41	0.0	2.3522792174249317E-4	0.0	0.32746863078844946	8.781842411719745E-4
42-43	0.0	2.509097831919927E-4	0.0	0.4365202953082693	8.781842411719745E-4
44-45	0.0	2.509097831919927E-4	0.0	0.5761202259317142	9.409116869699727E-4
46-47	1.5681861449499544E-5	2.509097831919927E-4	0.0	0.7308061072695777	0.0010193209942174703
48-49	3.136372289899909E-5	2.509097831919927E-4	0.0	0.8858997170051283	0.00103500285566697
50-51	3.136372289899909E-5	2.509097831919927E-4	0.0	1.0483638016219436	0.001066366578565969
52-53	3.136372289899909E-5	2.509097831919927E-4	0.0	1.221695416223262	0.0011290940243639672
54-55	3.136372289899909E-5	2.509097831919927E-4	0.0	1.4288998515554996	0.0011918214701619654
56-57	3.136372289899909E-5	2.509097831919927E-4	0.0	1.6647864114788717	0.0012075033316114648
58-59	3.136372289899909E-5	2.509097831919927E-4	0.0	1.913704598266778	0.0012388670545104641
60-61	3.136372289899909E-5	2.822735060909918E-4	0.0	2.178602601871724	0.0012859126388589626
62-63	3.136372289899909E-5	2.822735060909918E-4	0.0	2.4554658657626387	0.0013172763617579617
64-65	3.136372289899909E-5	2.822735060909918E-4	0.0	2.765417857311997	0.0013486400846569608
66-67	3.136372289899909E-5	3.7636467478798907E-4	0.0	3.1277629479641336	0.0013486400846569608
68-69	3.136372289899909E-5	3.7636467478798907E-4	0.0	3.516406520267081	0.0013486400846569608
70-71	3.136372289899909E-5	3.920465362374886E-4	0.0	3.917297626362087	0.001411367530454959
72-73	3.136372289899909E-5	4.0772839768698815E-4	0.0	4.338261515112453	0.0015681861449499544
74-75	3.136372289899909E-5	4.0772839768698815E-4	0.0	4.788519121050484	0.0015681861449499544
76-77	3.136372289899909E-5	4.0772839768698815E-4	0.0	5.302209856551741	0.0015681861449499544
78-79	3.136372289899909E-5	4.0772839768698815E-4	0.0	5.8662236854444405	0.0015681861449499544
80-81	3.136372289899909E-5	4.0772839768698815E-4	0.0	6.438235263676386	0.001646595452197452
82-83	3.136372289899909E-5	4.0772839768698815E-4	0.0	7.029896214304555	0.0019131870968389444
84-85	3.136372289899909E-5	4.0772839768698815E-4	0.0	7.654018618133187	0.0019131870968389444
86-87	3.136372289899909E-5	4.0772839768698815E-4	0.0	8.337151866596287	0.001928868958288444
88-89	3.136372289899909E-5	4.0772839768698815E-4	0.0	9.077978683332095	0.001975914542636943
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2885	0.0	53.53675	1
GTATCAA	5420	0.0	44.80599	1
TCAACGC	7275	0.0	33.203636	4
CAACGCA	7350	0.0	32.861603	5
TATCAAC	7420	0.0	32.81061	2
ATCAACG	7490	0.0	32.31389	3
AACGCAG	7795	0.0	30.86022	6
GTACATG	12520	0.0	30.366875	1
TACATGG	13120	0.0	28.61169	2
ACATGGG	13695	0.0	27.063868	3
ACGCAGA	9545	0.0	24.9034	7
CATGGGG	11075	0.0	24.89621	4
CGCAGAG	9915	0.0	23.542542	8
ATGGGGG	7485	0.0	23.248857	5
GCAGAGT	11105	0.0	19.607016	9
TGGGGGG	7195	0.0	18.236557	6
AGAGTAC	9640	0.0	17.877037	10-11
GTATACG	190	1.859741E-4	17.508902	1
GTGGTAT	1515	0.0	17.252996	1
TAAGTAC	3100	0.0	17.170021	1
>>END_MODULE