FastQCFastQC Report
Mon 15 Aug 2016
SRR2921374_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921374_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814984
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20700.048.8324781
GTATCAA36400.038.460961
TCAACGC41100.033.1401944
CAACGCA43100.031.4888925
GTACATG70600.031.0553721
ATCAACG44550.030.6792163
TATCAAC44900.030.2297532
AACGCAG46100.029.7416616
TACATGG73850.028.8543172
ACATGGG76900.026.4746633
ACGCAGA54250.024.5770027
CATGGGG67150.023.7469714
CGCAGAG56300.022.840548
GAGTACA52600.022.8262331
GTATACG1754.0847026E-621.694591
AGTACAT55250.020.1001132
ATGGGGG40850.019.1672825
GCAGAGT67400.017.6005259
GTACTAA5800.017.1826761
GTACTAG5300.017.0128571
Produced by FastQC (version 0.11.5)