##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921374_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2814984
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.5561569799331	31.0	31.0	34.0	27.0	34.0
2	30.600213002986873	33.0	31.0	34.0	27.0	34.0
3	30.654218283301077	33.0	31.0	34.0	26.0	34.0
4	34.117937437655065	37.0	35.0	37.0	30.0	37.0
5	34.11230863123911	37.0	35.0	37.0	30.0	37.0
6	34.159775330872215	37.0	35.0	37.0	30.0	37.0
7	34.0788910345494	37.0	35.0	37.0	30.0	37.0
8	34.04008122248652	37.0	35.0	37.0	30.0	37.0
9	35.621561969801604	39.0	37.0	39.0	30.0	39.0
10-11	35.51823793669875	39.0	37.0	39.0	30.0	39.0
12-13	35.41040588507785	39.0	37.0	39.0	30.0	39.0
14-15	36.573936299460314	40.0	37.0	41.0	29.5	41.0
16-17	36.52557865337778	40.0	37.0	41.0	29.0	41.0
18-19	36.468953642365285	40.0	37.0	41.0	29.0	41.0
20-21	36.4041365776857	40.0	37.0	41.0	29.0	41.0
22-23	36.28795296882682	40.0	37.0	41.0	28.0	41.0
24-25	36.193252785806244	40.0	36.5	41.0	27.0	41.0
26-27	36.04947203962793	40.0	36.0	41.0	27.0	41.0
28-29	35.915997923966884	40.0	36.0	41.0	27.0	41.0
30-31	35.77437598224359	40.0	36.0	41.0	27.0	41.0
32-33	35.602561684187194	40.0	36.0	41.0	26.0	41.0
34-35	35.441508015676106	39.5	35.0	41.0	25.0	41.0
36-37	35.271838845265194	39.0	35.0	41.0	25.0	41.0
38-39	35.513083378093796	39.5	35.5	41.0	25.5	41.0
40-41	35.639300791762935	40.0	36.0	41.0	26.0	41.0
42-43	35.4811132141426	40.0	35.0	41.0	25.0	41.0
44-45	35.368690905525575	40.0	35.0	41.0	24.5	41.0
46-47	35.22189522213981	40.0	35.0	41.0	24.0	41.0
48-49	35.0792532035706	40.0	35.0	41.0	24.0	41.0
50-51	33.347073198284605	37.0	32.5	39.5	20.5	40.0
52-53	33.50271884316216	37.5	33.5	39.5	21.0	40.0
54-55	34.362545932765514	39.0	34.0	40.5	22.0	41.0
56-57	34.36845147254834	39.0	34.0	41.0	22.0	41.0
58-59	34.18386161342302	38.5	34.0	41.0	21.0	41.0
60-61	33.93267226385656	38.0	34.0	40.0	20.0	41.0
62-63	33.63762227422963	37.0	34.0	40.0	19.5	41.0
64-65	33.33651789850315	37.0	34.0	40.0	18.0	41.0
66-67	32.98461998363046	36.0	33.0	39.0	16.0	41.0
68-69	32.613350555456094	36.0	33.0	39.0	13.0	41.0
70-71	32.22658956498509	35.0	33.0	39.0	9.5	40.5
72-73	31.87005894172045	35.0	32.5	37.5	7.5	40.0
74-75	31.46998295549815	35.0	32.0	37.0	4.0	39.0
76-77	31.094854002722574	35.0	32.0	36.5	2.0	39.0
78-79	30.750389167398467	35.0	32.0	36.0	2.0	38.5
80-81	30.4009532558622	35.0	31.5	35.5	2.0	37.0
82-83	30.099281381350657	35.0	31.0	35.0	2.0	37.0
84-85	29.86228198810366	35.0	31.0	35.0	2.0	36.0
86-87	29.630990620195355	35.0	31.0	35.0	2.0	36.0
88-89	29.42060736402054	35.0	31.0	35.0	2.0	36.0
90-91	29.15792008054042	35.0	31.0	35.0	2.0	35.5
92-93	28.958033154007268	34.5	30.5	35.0	2.0	35.0
94-95	28.738412545151235	34.0	30.0	35.0	2.0	35.0
96-97	28.473124714030348	34.0	30.0	35.0	2.0	35.0
98-99	28.225341067657933	34.0	30.0	35.0	2.0	35.0
100-101	27.16612971867691	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90679.0
3	15633.0
4	10871.0
5	8242.0
6	7488.0
7	7681.0
8	8322.0
9	8694.0
10	9353.0
11	10311.0
12	10056.0
13	9981.0
14	10931.0
15	11639.0
16	11619.0
17	12040.0
18	11385.0
19	11102.0
20	11160.0
21	12071.0
22	13377.0
23	15124.0
24	17623.0
25	20767.0
26	24992.0
27	29505.0
28	35263.0
29	41788.0
30	49926.0
31	60662.0
32	75054.0
33	97546.0
34	136073.0
35	198882.0
36	336774.0
37	616192.0
38	642683.0
39	113479.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.32901856877056	17.384058473234422	11.49998489476986	25.78693806322516
2	16.75512460057084	19.667483000934816	37.88127490323062	25.69611749526372
3	19.66891903418784	23.26352557832723	28.991673395810114	28.075881991674816
4	13.613279667197926	15.120791457018514	35.66557571432024	35.60035316146332
5	14.459424254497469	36.69202054705668	34.339870893174286	14.508684305271572
6	34.37893053828389	34.41925741694794	16.823165748800854	14.378646295967313
7	29.46842808000404	31.08317540445072	20.946841386818107	18.50155512872714
8	27.216145172671823	34.01375916975967	19.968703964661916	18.801391692906588
9	27.118550120398112	15.11196117982684	18.84204628105399	38.927442418721064
10-11	26.06245812801143	24.623705066281882	27.796675752736437	21.517161052970252
12-13	26.569053528270597	22.236621396916963	27.363914029701817	23.830411045110615
14-15	23.69681721118732	23.758567006702208	24.406344573810017	28.138271208300452
16-17	24.1333195460428	26.68677550097968	25.28482481257458	23.895080140402943
18-19	24.145942290944404	26.147676966584577	26.390112277786876	23.316268464684143
20-21	24.514476041337428	25.521226143984542	26.276417529550912	23.68788028512712
22-23	24.399852861994145	25.356960711664595	26.056079482955624	24.18710694338563
24-25	24.18526441035643	25.548247991081187	26.0658882013713	24.200599397191088
26-27	24.01304118010243	25.751373632475026	26.05814340856294	24.177441778859606
28-29	24.179666181553575	25.863699091549936	25.913761909905404	24.042872816991085
30-31	24.417029632546896	25.57109503692899	25.790304578690453	24.22157075183366
32-33	24.088433003249676	25.739277610266807	25.775000257572543	24.397289128910973
34-35	24.43539764593366	25.719189987446473	25.840817281414154	24.004595085205718
36-37	24.467937716678456	25.528705199359276	25.900986272190945	24.102370811771323
38-39	24.23365677109932	25.716100095477053	25.904016697382154	24.146226436041477
40-41	24.355219375240573	25.51148063788604	25.972883857157548	24.160416129715834
42-43	24.201791927639388	25.482477656083464	25.884394038964174	24.431336377312977
44-45	24.098917581790847	25.594518561727487	25.9871836644484	24.31938019203327
46-47	24.310885771386165	25.56521692705506	25.819329189186302	24.30456811237247
48-49	24.091663227631724	25.67739200673962	25.815291524278162	24.415653241350494
50-51	24.089429635601018	25.681452969094508	25.996398185568186	24.23271920973629
52-53	24.338678292673986	25.46969048211412	25.92700359450918	24.264627630702712
54-55	23.870228575638603	25.480661599072047	26.142958275095857	24.506151550193497
56-57	23.91920269858274	25.644384873029175	26.03850068690841	24.397911741479675
58-59	24.050475438580253	25.498895496058598	26.015784119490025	24.43484494587112
60-61	24.00815376028386	25.520707261993923	26.168191862898933	24.302947114823283
62-63	24.031525542823033	25.482168238031623	26.211022442629783	24.27528377651556
64-65	23.899446962565634	25.36012929895257	26.114462178970694	24.625961559511104
66-67	23.87460952953481	25.548020059143827	25.970270055273158	24.6071003560482
68-69	23.967231295771118	25.464402455552896	26.04446062687213	24.523905621803852
70-71	23.871246808562884	25.249901381056656	26.108513399276205	24.770338411104255
72-73	23.901214075791298	25.43416537538694	26.004956094116675	24.659664454705084
74-75	23.883639332395447	25.555302083692926	26.03450625954894	24.526552324362683
76-77	23.94361259944016	25.297868959544555	26.056876738595797	24.701641702419483
78-79	23.91337391157626	25.361852725824903	26.042005209269607	24.682768153329228
80-81	24.02154158886573	25.454301108763488	25.911637121593746	24.612520180777043
82-83	23.996413703539947	25.43949397406788	26.052571247115548	24.511521075276626
84-85	23.98451915784475	25.51656649103225	25.958586397769146	24.540327953353852
86-87	23.962471024847645	25.60062079503136	25.927271633252413	24.50963654686858
88-89	23.903609765059173	25.358164735395395	25.97874803492043	24.759477464625007
90-91	24.00146115261942	25.386102637070728	25.971720786693968	24.64071542361588
92-93	24.087357473456493	25.245372009655192	26.0997702271577	24.567500289730614
94-95	24.06567588922673	25.146838002298132	26.10018562388748	24.687300484587663
96-97	24.08099323519295	24.99015438914839	26.126441144611906	24.802411231046754
98-99	23.948242418373074	25.073736965184473	26.023897043115653	24.9541235733268
100-101	24.221330322801872	25.006022190503472	26.06710278067636	24.705544706018294
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	93.0
1	74.5
2	96.5
3	144.0
4	131.5
5	152.0
6	242.0
7	373.5
8	557.0
9	652.5
10	589.0
11	525.0
12	546.5
13	598.5
14	669.0
15	748.5
16	818.5
17	876.0
18	961.0
19	1076.5
20	1171.0
21	1288.0
22	1437.0
23	1565.0
24	1745.0
25	2001.5
26	2524.5
27	3391.5
28	4534.0
29	6380.0
30	9301.5
31	13455.0
32	19660.0
33	24920.0
34	29133.0
35	35080.5
36	43170.5
37	51405.0
38	58745.5
39	66494.0
40	73564.0
41	79968.0
42	86164.5
43	92827.0
44	99595.0
45	107114.5
46	113157.5
47	117314.0
48	121442.5
49	124016.0
50	124659.0
51	124899.0
52	124613.5
53	123649.0
54	121921.5
55	116823.0
56	108928.0
57	99285.5
58	89889.0
59	78921.0
60	65703.0
61	54395.0
62	44265.5
63	33866.5
64	24765.5
65	18182.5
66	13730.5
67	10691.0
68	8201.5
69	6300.0
70	4885.0
71	3609.0
72	2612.0
73	1968.5
74	1546.5
75	1209.0
76	874.0
77	578.5
78	368.0
79	251.5
80	185.0
81	133.0
82	96.0
83	83.0
84	72.0
85	54.5
86	45.5
87	43.5
88	39.0
89	32.0
90	26.5
91	22.5
92	21.0
93	22.5
94	15.5
95	13.5
96	13.5
97	10.0
98	7.0
99	5.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04934308685235866
2	0.05662554387520498
3	0.048028692170186404
4	0.05470723812284546
5	0.04778002290599165
6	0.017193703410037144
7	0.015133300935280626
8	0.02024878294157267
9	0.02028430712217192
10-11	0.021403318811048302
12-13	0.027691098777115605
14-15	0.042966496434793236
16-17	0.02834829611820174
18-19	0.022202612874531437
20-21	0.025950413927752342
22-23	0.04614591059842613
24-25	0.0411014769533326
26-27	0.02918311436228412
28-29	0.018472573911610154
30-31	0.011563120785055973
32-33	0.008632375885617822
34-35	0.008436992892321945
36-37	0.001456491404569262
38-39	0.006092396972771426
40-41	0.0032859867054306525
42-43	0.21108468112074527
44-45	0.18509874301239368
46-47	0.19179505105535236
48-49	0.18932612050370445
50-51	0.18788739118943484
52-53	0.19257658302853586
54-55	0.19065827727617635
56-57	0.20097805174025857
58-59	0.19815387938261816
60-61	0.2034825064725057
62-63	0.20046295112156942
64-65	0.20179510789404131
66-67	0.19156414388145723
68-69	0.1901254145671876
70-71	0.17497435154160734
72-73	0.181528562862169
74-75	0.18177723212636376
76-77	0.17994773682550239
78-79	0.20465480443228098
80-81	0.21310955941490256
82-83	0.21165306801033326
84-85	0.20726583170632587
86-87	0.22477925274175625
88-89	0.2222215117386102
90-91	0.22175969739082
92-93	0.22524106708954653
94-95	0.23506350302523923
96-97	0.23538322065063247
98-99	0.2256495951664379
100-101	0.23518783765733658
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2814984.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.65481169104786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.33099174688229	46.72820562233287
2	11.87128069987779	14.16358029365561
3	3.919717149599528	7.014899653245922
4	1.820071646337322	4.343041253058737
5	1.0383789486296366	3.097215032222462
6	0.6371834913767506	2.2806636714434685
7	0.46082357055578493	1.924324032701116
8	0.3175951624110804	1.515686368609661
9	0.2562672077738373	1.3758814820105012
>10	1.2770332053604756	13.267237541305931
>50	0.05480096355966916	2.1751366191951567
>100	0.01454226005703646	1.422502734818099
>500	8.361484593469649E-4	0.35308111695201466
>1k	4.7779911945845257E-4	0.33854457844837293
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.55241805992503E-5
2	0.0	0.0	0.0	0.0	3.55241805992503E-5
3	0.0	0.0	0.0	0.0	3.55241805992503E-5
4	0.0	0.0	0.0	0.0	1.0657254179775089E-4
5	0.0	0.0	0.0	0.0	1.0657254179775089E-4
6	0.0	0.0	0.0	0.0	1.0657254179775089E-4
7	0.0	0.0	0.0	0.0	1.0657254179775089E-4
8	0.0	0.0	0.0	0.0	1.0657254179775089E-4
9	0.0	0.0	0.0	3.55241805992503E-5	1.0657254179775089E-4
10-11	0.0	0.0	0.0	3.55241805992503E-5	1.0657254179775089E-4
12-13	3.55241805992503E-5	3.55241805992503E-5	0.0	5.3286270898875446E-5	1.0657254179775089E-4
14-15	3.55241805992503E-5	3.55241805992503E-5	0.0	7.10483611985006E-5	1.5985881269662633E-4
16-17	3.55241805992503E-5	3.55241805992503E-5	0.0	7.10483611985006E-5	2.1314508359550178E-4
18-19	3.55241805992503E-5	3.55241805992503E-5	0.0	7.10483611985006E-5	2.6643135449437724E-4
20-21	3.55241805992503E-5	3.55241805992503E-5	0.0	1.2433463209737605E-4	2.841934447940024E-4
22-23	7.10483611985006E-5	3.55241805992503E-5	0.0	3.019555350936275E-4	3.019555350936275E-4
24-25	7.10483611985006E-5	3.55241805992503E-5	0.0	6.03911070187255E-4	3.1971762539325266E-4
26-27	7.10483611985006E-5	3.55241805992503E-5	0.0	0.0015808260366666383	3.1971762539325266E-4
28-29	7.10483611985006E-5	3.55241805992503E-5	0.0	0.007033787758651559	3.1971762539325266E-4
30-31	7.10483611985006E-5	3.55241805992503E-5	0.0	0.02849039284059874	3.1971762539325266E-4
32-33	7.10483611985006E-5	3.55241805992503E-5	0.0	0.0705510226701111	3.55241805992503E-4
34-35	1.0657254179775089E-4	3.55241805992503E-5	0.0	0.1253648333347543	3.55241805992503E-4
36-37	1.0657254179775089E-4	5.3286270898875446E-5	0.0	0.20167077326194394	3.55241805992503E-4
38-39	1.0657254179775089E-4	7.10483611985006E-5	0.0	0.3097175685545637	3.55241805992503E-4
40-41	1.0657254179775089E-4	8.881045149812574E-5	0.0	0.4321694190801795	3.55241805992503E-4
42-43	1.0657254179775089E-4	1.0657254179775089E-4	0.0	0.565154189153473	3.55241805992503E-4
44-45	1.0657254179775089E-4	1.0657254179775089E-4	0.0	0.7348709619663913	3.55241805992503E-4
46-47	1.0657254179775089E-4	1.0657254179775089E-4	0.0	0.924090509928298	3.55241805992503E-4
48-49	1.0657254179775089E-4	1.0657254179775089E-4	0.0	1.110254978358669	3.55241805992503E-4
50-51	1.0657254179775089E-4	1.0657254179775089E-4	0.0	1.3042880527917742	3.55241805992503E-4
52-53	1.0657254179775089E-4	1.0657254179775089E-4	0.0	1.5061719711373138	3.907659865917533E-4
54-55	1.0657254179775089E-4	1.0657254179775089E-4	0.0	1.7413065225237514	4.7957643808987905E-4
56-57	1.0657254179775089E-4	1.420967223970012E-4	0.0	2.014025656984196	5.151006186891293E-4
58-59	1.0657254179775089E-4	1.9538299329587664E-4	0.0	2.296478416928835	5.328627089887545E-4
60-61	1.0657254179775089E-4	2.1314508359550178E-4	0.0	2.584899239214148	5.328627089887545E-4
62-63	1.0657254179775089E-4	2.1314508359550178E-4	0.0	2.8896967087557157	5.328627089887545E-4
64-65	1.0657254179775089E-4	2.1314508359550178E-4	0.0	3.21788685122189	5.328627089887545E-4
66-67	1.0657254179775089E-4	2.1314508359550178E-4	0.0	3.602222961125179	5.328627089887545E-4
68-69	1.0657254179775089E-4	2.1314508359550178E-4	0.0	4.007411765040228	5.328627089887545E-4
70-71	1.0657254179775089E-4	2.1314508359550178E-4	0.0	4.428728546947337	5.328627089887545E-4
72-73	1.0657254179775089E-4	2.1314508359550178E-4	0.0	4.864556956629238	5.861489798876299E-4
74-75	1.0657254179775089E-4	2.486692641947521E-4	0.0	5.329426383951027	6.03911070187255E-4
76-77	1.0657254179775089E-4	2.841934447940024E-4	0.0	5.850441778709932	6.03911070187255E-4
78-79	1.0657254179775089E-4	2.841934447940024E-4	0.0	6.4218126994682745	6.03911070187255E-4
80-81	1.0657254179775089E-4	2.841934447940024E-4	0.0	7.005101982817664	6.03911070187255E-4
82-83	1.0657254179775089E-4	3.1971762539325266E-4	0.0	7.605886925112186	6.749594313857556E-4
84-85	1.0657254179775089E-4	3.1971762539325266E-4	0.0	8.23022439914401	6.749594313857556E-4
86-87	1.0657254179775089E-4	3.1971762539325266E-4	0.0	8.910814413154746	6.749594313857556E-4
88-89	1.0657254179775089E-4	3.374797156928778E-4	0.0	9.638829208265483	6.749594313857556E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2070	0.0	48.832478	1
GTATCAA	3640	0.0	38.46096	1
TCAACGC	4110	0.0	33.140194	4
CAACGCA	4310	0.0	31.488892	5
GTACATG	7060	0.0	31.055372	1
ATCAACG	4455	0.0	30.679216	3
TATCAAC	4490	0.0	30.229753	2
AACGCAG	4610	0.0	29.741661	6
TACATGG	7385	0.0	28.854317	2
ACATGGG	7690	0.0	26.474663	3
ACGCAGA	5425	0.0	24.577002	7
CATGGGG	6715	0.0	23.746971	4
CGCAGAG	5630	0.0	22.84054	8
GAGTACA	5260	0.0	22.826233	1
GTATACG	175	4.0847026E-6	21.69459	1
AGTACAT	5525	0.0	20.100113	2
ATGGGGG	4085	0.0	19.167282	5
GCAGAGT	6740	0.0	17.600525	9
GTACTAA	580	0.0	17.182676	1
GTACTAG	530	0.0	17.012857	1
>>END_MODULE