FastQCFastQC Report
Mon 15 Aug 2016
SRR2921374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814984
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21750.045.671011
GTATCAA35350.040.0664141
TCAACGC42000.031.9801644
CAACGCA44400.030.6260665
GTACATG68450.029.4405561
TATCAAC46150.029.41292
ATCAACG45750.029.3588373
AACGCAG47250.028.5760026
TACATGG72200.026.4917952
ACATGGG73950.025.8006973
ACGCAGA58900.022.6010027
CATGGGG66150.022.242084
CGCAGAG61500.021.3362928
ATGGGGG38700.019.7798545
AGAGTAC56950.017.53115510-11
GTATAGA6250.017.4904671
GCAGAGT71400.017.0461679
GAGTACA52950.016.8750651
TAAGGTG11500.016.5375185
ACCGTCG2304.2415857E-516.5366348
Produced by FastQC (version 0.11.5)