##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921374_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2814984
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48137857977168	33.0	31.0	34.0	30.0	34.0
2	31.599954031710304	34.0	31.0	34.0	29.0	34.0
3	31.685650788778904	34.0	31.0	34.0	29.0	34.0
4	35.163696845168566	37.0	35.0	37.0	33.0	37.0
5	34.90469928070639	37.0	35.0	37.0	32.0	37.0
6	34.86186635519065	37.0	35.0	37.0	32.0	37.0
7	34.724893640603284	37.0	35.0	37.0	32.0	37.0
8	34.68953358171841	37.0	35.0	37.0	32.0	37.0
9	36.32989246120049	39.0	37.0	39.0	32.0	39.0
10-11	36.18023086454488	39.0	37.0	39.0	32.0	39.0
12-13	36.11196244809918	39.0	37.0	39.0	32.0	39.0
14-15	37.37741724286887	40.0	38.0	41.0	32.0	41.0
16-17	37.3511391183751	40.0	38.0	41.0	32.0	41.0
18-19	37.30420457096737	40.0	38.0	41.0	31.5	41.0
20-21	37.261324220670524	40.0	38.0	41.0	31.0	41.0
22-23	37.15387778403003	40.0	38.0	41.0	31.0	41.0
24-25	37.08795076632762	40.0	38.0	41.0	31.0	41.0
26-27	36.95167734523535	40.0	37.0	41.0	30.5	41.0
28-29	36.800545047502936	40.0	37.0	41.0	30.0	41.0
30-31	36.66773860881625	40.0	37.0	41.0	30.0	41.0
32-33	36.62635489224806	40.0	37.0	41.0	30.0	41.0
34-35	36.766601337698546	40.0	37.0	41.0	30.0	41.0
36-37	36.74873977969324	40.0	37.0	41.0	30.0	41.0
38-39	36.658536602694724	40.0	37.0	41.0	30.0	41.0
40-41	36.53830341486844	40.0	37.0	41.0	30.0	41.0
42-43	36.4403989507578	40.0	37.0	41.0	30.0	41.0
44-45	36.31123658251698	40.0	36.5	41.0	29.0	41.0
46-47	36.16476878731815	40.0	36.0	41.0	28.5	41.0
48-49	36.007409811210294	40.0	36.0	41.0	28.0	41.0
50-51	35.847363786081914	40.0	35.0	41.0	28.0	41.0
52-53	35.668637015343606	39.0	35.0	41.0	28.0	41.0
54-55	35.45175905085073	39.0	35.0	41.0	27.0	41.0
56-57	35.17787898616831	39.0	35.0	41.0	26.0	41.0
58-59	34.94491300838655	38.5	35.0	40.5	26.0	41.0
60-61	34.659497887021736	38.0	34.5	40.0	26.0	41.0
62-63	34.350797375757736	37.0	34.0	40.0	26.0	41.0
64-65	34.02782538017978	37.0	34.0	40.0	26.0	41.0
66-67	33.67871380441238	36.0	34.0	39.0	25.0	41.0
68-69	33.310433203172735	36.0	34.0	39.0	24.0	41.0
70-71	32.928476502885985	35.0	33.5	38.5	23.5	40.0
72-73	32.53154209757498	35.0	33.0	37.0	22.0	39.5
74-75	32.12979736296903	35.0	33.0	37.0	21.5	39.0
76-77	29.384658314221323	33.0	29.0	34.5	15.0	36.5
78-79	31.047717145106333	34.5	31.5	36.0	19.0	37.0
80-81	31.252163422598493	35.0	32.0	35.5	20.0	37.0
82-83	31.09965438524695	35.0	32.0	35.0	19.0	37.0
84-85	30.878101793829025	35.0	32.0	35.0	18.0	36.0
86-87	30.641936153100694	35.0	32.0	35.0	16.0	36.0
88-89	30.370523953244493	35.0	32.0	35.0	8.0	36.0
90-91	30.1983602748719	35.0	32.0	35.0	4.5	35.0
92-93	30.061746709750395	34.5	32.0	35.0	2.0	35.0
94-95	29.974147277568896	34.0	32.0	35.0	2.0	35.0
96-97	29.787911760777327	34.0	31.0	35.0	2.0	35.0
98-99	29.527973160771072	34.0	31.0	35.0	2.0	35.0
100-101	28.433494826258336	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50809.0
3	17645.0
4	9852.0
5	6784.0
6	6040.0
7	5935.0
8	6061.0
9	6642.0
10	7436.0
11	7802.0
12	8485.0
13	9013.0
14	9738.0
15	10274.0
16	10865.0
17	11822.0
18	11321.0
19	10491.0
20	9951.0
21	9771.0
22	10508.0
23	11852.0
24	13894.0
25	16601.0
26	20002.0
27	23941.0
28	29126.0
29	35453.0
30	42638.0
31	53521.0
32	67861.0
33	90025.0
34	129593.0
35	196362.0
36	353575.0
37	691243.0
38	713230.0
39	88820.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.50038957074641	18.576796668262897	11.988995249015757	26.933818511974945
2	15.787974186654388	20.800269131765862	36.3842275143891	27.02752916719065
3	18.4016955890456	24.122887574502645	28.764939163353564	28.71047767309819
4	13.292535320797644	16.063844413347386	34.707086682987295	35.936533582867675
5	14.339110560898963	36.973131201434974	34.24499727815546	14.44276095951061
6	34.52805133739688	34.84702726091371	16.505822847045735	14.119098554643678
7	29.42930738835723	31.256849508505024	20.883039080520202	18.430804022617544
8	27.246225753863413	34.383056364041344	19.641675308080156	18.729042574015082
9	26.97866133519764	14.937385079275762	18.698010361692997	39.3859432238336
10-11	26.023209958199317	24.821178064509247	27.672034436224173	21.48357754106726
12-13	26.457276042654687	22.32994360879552	27.347247474047755	23.865532874502037
14-15	23.622484605261075	23.850739020296523	24.288578948406155	28.238197426036244
16-17	24.141520821832355	26.872625647518383	25.12255868429522	23.863294846354037
18-19	24.12583990577531	26.310318729465838	26.233017824146483	23.33082354061237
20-21	24.433651324600476	25.59327621474109	26.26523910447104	23.7078333561874
22-23	24.288253762551605	25.46559866542474	26.012636688457246	24.23351088356641
24-25	24.124693177460347	25.69936178069775	25.981889854549372	24.194055187292523
26-27	24.01498521976037	25.84463714076522	26.02419902327146	24.11617861620295
28-29	24.15072722234459	25.93913642790658	25.863323409667405	24.046812940081427
30-31	24.335016639128714	25.693397695338998	25.749657899939976	24.221927765592312
32-33	24.035276054274842	25.8470251817265	25.727505555406534	24.390193208592127
34-35	24.343452879086826	25.80580981383776	25.84002343437602	24.010713872699398
36-37	24.359596719603392	25.69066146304865	25.89117908946987	24.058562727878098
38-39	24.230522542179276	25.8250285037701	25.870250874029765	24.07419808002086
40-41	24.33642906219381	25.62857790443953	25.926462762083606	24.108530271283048
42-43	24.197993905617	25.59738560419622	25.793657137842697	24.410963352344083
44-45	24.083927924644065	25.713789349208287	25.90471226825416	24.297570457893485
46-47	24.257629320122447	25.729607554775303	25.74287694162482	24.26988618347743
48-49	24.05769147757138	25.800987779677133	25.788499754769294	24.352820987982184
50-51	24.07886974888657	25.813300099987796	25.91106752471319	24.196762626412447
52-53	24.33150031714691	25.564865202349672	25.811175827277406	24.29245865322601
54-55	23.800522566080097	25.586412571426543	26.05233476201218	24.56073010048118
56-57	23.879039426350126	25.761807690586036	25.859291790801393	24.49986109226245
58-59	23.964008325557213	25.644183505456077	25.880851165121477	24.510957003865233
60-61	23.940738745962573	25.708067465160838	25.982638285052367	24.368555503824222
62-63	23.902151310337107	25.677863950206927	25.969342117769116	24.45064262168685
64-65	23.7591768867011	25.51628483165772	25.920105159952083	24.804433121689097
66-67	23.703546680493133	25.737910045285467	25.79011466893064	24.768428605290755
68-69	23.79102144547433	25.576281717236988	25.84662656148755	24.786070275801134
70-71	23.66695293551736	25.379892625191978	25.89023659738463	25.062917841906028
72-73	23.686280376382246	25.557993378967158	25.76478294912593	24.990943295524666
74-75	23.527105208407917	25.657085059013507	25.794387342747754	25.021422389830818
76-77	23.62971059378065	25.336731374616782	25.90268186859273	25.130876163009834
78-79	23.537085064038383	25.335602290689486	25.890873555099386	25.236439090172745
80-81	23.566170479080874	25.481758224025324	25.753900459529927	25.19817083736387
82-83	23.49069038253438	25.419449330570608	25.86494796623548	25.224912320659527
84-85	23.43381283063201	25.43822039865694	25.80875746062533	25.319209310085718
86-87	23.309582129196524	25.54430361295261	25.765123505017158	25.38099075283371
88-89	23.208201155636164	25.318116958472753	25.800866833982912	25.672815051908167
90-91	23.196912908627812	25.23644809154075	25.890081528663327	25.676557471168103
92-93	23.183039881031505	25.098559881258982	25.984301504379925	25.734098733329592
94-95	23.062002250557978	24.971335138634903	26.05943640130984	25.907226209497285
96-97	22.964546702208427	24.868858929689914	26.083332711601205	26.083261656500454
98-99	22.84141814241816	24.86930496101174	25.997264605055154	26.29201229151495
100-101	22.75087972583425	24.867479389876763	26.0535972442747	26.328043640014286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	95.0
1	71.5
2	85.5
3	122.5
4	101.5
5	114.5
6	198.0
7	331.0
8	461.5
9	513.5
10	453.0
11	378.0
12	377.5
13	401.5
14	423.0
15	444.5
16	482.0
17	553.0
18	624.0
19	668.5
20	742.0
21	851.5
22	977.5
23	1162.0
24	1411.0
25	1772.0
26	2326.5
27	3170.5
28	4459.0
29	6444.0
30	9429.0
31	13743.5
32	19941.0
33	25016.0
34	29106.0
35	34962.0
36	42934.0
37	50958.0
38	58194.0
39	65634.5
40	72534.0
41	79216.5
42	85383.0
43	92266.0
44	99412.0
45	106822.0
46	112974.0
47	117221.0
48	121893.5
49	125031.0
50	125504.0
51	125854.5
52	126251.5
53	125687.5
54	123957.0
55	118999.5
56	110837.5
57	100918.5
58	91087.0
59	79533.0
60	66180.5
61	54333.5
62	44055.5
63	33794.5
64	24167.0
65	17486.0
66	13245.5
67	10277.5
68	7863.0
69	5934.5
70	4461.5
71	3289.0
72	2379.5
73	1787.0
74	1404.5
75	1060.0
76	731.5
77	420.5
78	242.0
79	147.0
80	86.5
81	51.0
82	29.0
83	19.5
84	17.0
85	15.0
86	11.5
87	7.0
88	5.0
89	5.0
90	3.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14913051015565276
2	2.1314508359550176E-4
3	0.0053641512704867955
4	0.005008909464494292
5	0.025435313309063214
6	0.0013499188627715112
7	3.55241805992503E-5
8	0.0018117332105617651
9	0.0
10-11	0.1004979069152791
12-13	2.1314508359550176E-4
14-15	0.005062195735393168
16-17	2.1314508359550176E-4
18-19	3.7300389629212814E-4
20-21	7.815319731835066E-4
22-23	2.1314508359550176E-4
24-25	0.0015097776754681375
26-27	0.002735361906142273
28-29	0.005825965618277048
30-31	0.013623523259812488
32-33	0.013623523259812488
34-35	0.011207878979063468
36-37	6.927215216853808E-4
38-39	1.9538299329587664E-4
40-41	0.004902336922696541
42-43	0.009271811136404327
44-45	0.008365944531123446
46-47	0.008330420350524195
48-49	0.01021320192228446
50-51	0.004991147374194667
52-53	0.0015097776754681375
54-55	0.0010302012373782587
56-57	0.00589701397947555
58-59	0.009857960116291958
60-61	0.008081751086329442
62-63	0.019058722891497785
64-65	0.03499131789026154
66-67	0.037549058893407566
68-69	0.028437106569699864
70-71	0.04246915790640373
72-73	0.027371381151722357
74-75	0.0268385184427336
76-77	0.23799424792467738
78-79	0.0690767691752422
80-81	0.05561310472812634
82-83	0.02165198807524306
84-85	0.034120975465579906
86-87	0.08115499057898731
88-89	0.2673549831899577
90-91	0.3189005692394699
92-93	0.05207844875850094
94-95	0.004493808845805163
96-97	0.00948495621999983
98-99	0.00120782214037451
100-101	0.0015808260366666383
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2814984.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.88363434790528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6532389339977	45.72504927320043
2	12.225871399934034	14.39807482196457
3	4.069680265940524	7.1891269407758305
4	1.87192099729333	4.409020461311465
5	1.0550766043996362	3.106337249124885
6	0.6732807643825401	2.3787131006027797
7	0.4551869653168268	1.876214397795407
8	0.3340275722963261	1.5735005943372293
9	0.25382013607767706	1.3451266874640146
>10	1.3354946272599788	13.657952304150253
>50	0.05607946998997357	2.2050828766904966
>100	0.01505013968745949	1.4601735628250463
>500	7.875049767400394E-4	0.3467848998881264
>1k	4.84618447209116E-4	0.3288428298694672
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	3.55241805992503E-5	0.0	0.0	0.0
8	0.0	3.55241805992503E-5	0.0	0.0	0.0
9	0.0	3.55241805992503E-5	0.0	0.0	3.55241805992503E-5
10-11	0.0	3.55241805992503E-5	0.0	0.0	3.55241805992503E-5
12-13	0.0	7.10483611985006E-5	0.0	1.776209029962515E-5	3.55241805992503E-5
14-15	0.0	1.0657254179775089E-4	0.0	3.55241805992503E-5	3.55241805992503E-5
16-17	0.0	1.0657254179775089E-4	0.0	3.55241805992503E-5	3.55241805992503E-5
18-19	0.0	1.0657254179775089E-4	0.0	3.55241805992503E-5	3.55241805992503E-5
20-21	0.0	1.0657254179775089E-4	0.0	8.881045149812574E-5	3.55241805992503E-5
22-23	0.0	1.0657254179775089E-4	0.0	2.6643135449437724E-4	7.10483611985006E-5
24-25	0.0	1.2433463209737605E-4	0.0	5.328627089887545E-4	1.0657254179775089E-4
26-27	0.0	1.420967223970012E-4	0.0	0.0014920155851685124	1.2433463209737605E-4
28-29	0.0	1.420967223970012E-4	0.0	0.006927215216853808	1.420967223970012E-4
30-31	0.0	1.776209029962515E-4	0.0	0.02859696538239649	1.420967223970012E-4
32-33	1.776209029962515E-5	1.776209029962515E-4	0.0	0.07117269583059797	1.420967223970012E-4
34-35	3.55241805992503E-5	1.776209029962515E-4	0.0	0.12637727248183295	1.420967223970012E-4
36-37	3.55241805992503E-5	1.776209029962515E-4	0.0	0.20314502675681284	1.420967223970012E-4
38-39	3.55241805992503E-5	1.776209029962515E-4	0.0	0.3112451083203315	1.420967223970012E-4
40-41	3.55241805992503E-5	1.776209029962515E-4	0.0	0.43362591048474874	1.420967223970012E-4
42-43	3.55241805992503E-5	1.776209029962515E-4	0.0	0.565349572146769	1.5985881269662633E-4
44-45	3.55241805992503E-5	1.776209029962515E-4	0.0	0.734551244340998	1.776209029962515E-4
46-47	3.55241805992503E-5	1.776209029962515E-4	0.0	0.9246233726372868	1.776209029962515E-4
48-49	3.55241805992503E-5	1.776209029962515E-4	0.0	1.110450361351965	1.776209029962515E-4
50-51	3.55241805992503E-5	1.776209029962515E-4	0.0	1.3041814802499765	1.776209029962515E-4
52-53	3.55241805992503E-5	1.776209029962515E-4	0.0	1.5066515475754036	1.9538299329587664E-4
54-55	3.55241805992503E-5	2.3090717389512695E-4	0.0	1.743047207373115	2.1314508359550178E-4
56-57	3.55241805992503E-5	2.841934447940024E-4	0.0	2.013421745914009	2.3090717389512695E-4
58-59	3.55241805992503E-5	2.841934447940024E-4	0.0	2.29480878044067	2.486692641947521E-4
60-61	3.55241805992503E-5	2.841934447940024E-4	0.0	2.5834249857192795	2.486692641947521E-4
62-63	3.55241805992503E-5	2.841934447940024E-4	0.0	2.8878494513645547	2.841934447940024E-4
64-65	3.55241805992503E-5	3.019555350936275E-4	0.0	3.216057355921028	2.841934447940024E-4
66-67	3.55241805992503E-5	3.1971762539325266E-4	0.0	3.59883040187795	2.841934447940024E-4
68-69	3.55241805992503E-5	3.1971762539325266E-4	0.0	4.000004973385284	2.841934447940024E-4
70-71	3.55241805992503E-5	3.374797156928778E-4	0.0	4.420504699138609	3.1971762539325266E-4
72-73	3.55241805992503E-5	3.7300389629212814E-4	0.0	4.858624418469164	3.374797156928778E-4
74-75	3.55241805992503E-5	3.907659865917533E-4	0.0	5.326673259954585	3.55241805992503E-4
76-77	3.55241805992503E-5	4.2629016719100357E-4	0.0	5.849678008827048	3.55241805992503E-4
78-79	3.55241805992503E-5	4.2629016719100357E-4	0.0	6.425471690069997	3.55241805992503E-4
80-81	3.55241805992503E-5	4.2629016719100357E-4	0.0	7.012935064639799	3.7300389629212814E-4
82-83	3.55241805992503E-5	4.2629016719100357E-4	0.0	7.616881659007653	4.2629016719100357E-4
84-85	3.55241805992503E-5	4.2629016719100357E-4	0.0	8.245162317085995	4.440522574906287E-4
86-87	5.3286270898875446E-5	4.2629016719100357E-4	0.0	8.932235494056094	4.973385283895042E-4
88-89	7.10483611985006E-5	4.2629016719100357E-4	0.0	9.665774299250014	4.973385283895042E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2175	0.0	45.67101	1
GTATCAA	3535	0.0	40.066414	1
TCAACGC	4200	0.0	31.980164	4
CAACGCA	4440	0.0	30.626066	5
GTACATG	6845	0.0	29.440556	1
TATCAAC	4615	0.0	29.4129	2
ATCAACG	4575	0.0	29.358837	3
AACGCAG	4725	0.0	28.576002	6
TACATGG	7220	0.0	26.491795	2
ACATGGG	7395	0.0	25.800697	3
ACGCAGA	5890	0.0	22.601002	7
CATGGGG	6615	0.0	22.24208	4
CGCAGAG	6150	0.0	21.336292	8
ATGGGGG	3870	0.0	19.779854	5
AGAGTAC	5695	0.0	17.531155	10-11
GTATAGA	625	0.0	17.490467	1
GCAGAGT	7140	0.0	17.046167	9
GAGTACA	5295	0.0	16.875065	1
TAAGGTG	1150	0.0	16.537518	5
ACCGTCG	230	4.2415857E-5	16.536634	8
>>END_MODULE