FastQCFastQC Report
Mon 15 Aug 2016
SRR2921373_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921373_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428747
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18750.050.6241341
GTATCAA31650.039.8874971
TCAACGC37800.032.8983544
CAACGCA39000.031.8834675
ATCAACG40000.030.9670963
GTACATG58650.030.6690331
AACGCAG41100.030.12966
TATCAAC41600.029.890752
TACATGG61750.028.2070672
ACATGGG63600.026.7144263
CATGGGG52300.024.412674
ACGCAGA50150.024.317037
CGCAGAG52850.022.6271978
GAGTACA44200.021.0456681
GCAGAGT59800.018.6484139
GTATAGG6300.018.0800481
AGTACAT45800.017.9270782
ATGGGGG32050.017.7697815
CAGAGTA54450.017.08196410-11
AGAGTAC49800.016.7235110-11
Produced by FastQC (version 0.11.5)