FastQCFastQC Report
Mon 15 Aug 2016
SRR2921373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428747
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.047.269451
GTATCAA30700.039.014171
TCAACGC37150.031.0455324
GTACATG56000.030.893961
CAACGCA38650.030.1371545
ATCAACG40200.028.9262183
AACGCAG41050.028.6038596
TACATGG60550.027.9837362
TATCAAC41800.027.932542
ACATGGG63000.026.292783
CATGGGG46850.025.4282074
ACGCAGA49850.023.076677
CGCAGAG52500.021.8213028
ATGGGGG26950.020.6401445
GAGTACA42000.018.4458451
AGAGTAC51000.017.42987810-11
GCAGAGT61300.017.2928819
GTACCGT2807.08078E-716.9778566
GTGTAGC14450.016.1171571
AGTACAT45500.015.8556412
Produced by FastQC (version 0.11.5)