##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921373_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2428747
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.348976241658765	33.0	31.0	34.0	28.0	34.0
2	31.469650811714846	34.0	31.0	34.0	28.0	34.0
3	31.54963485286858	34.0	31.0	34.0	28.0	34.0
4	35.04051924716737	37.0	35.0	37.0	32.0	37.0
5	34.7726234968072	37.0	35.0	37.0	32.0	37.0
6	34.72713337371081	37.0	35.0	37.0	32.0	37.0
7	34.588022959987185	37.0	35.0	37.0	32.0	37.0
8	34.55385637120705	37.0	35.0	37.0	32.0	37.0
9	36.17631107727565	39.0	37.0	39.0	32.0	39.0
10-11	36.02150676871655	39.0	37.0	39.0	31.0	39.0
12-13	35.95776217119362	39.0	37.0	39.0	31.0	39.0
14-15	37.19411861342495	40.0	38.0	41.0	31.0	41.0
16-17	37.167246732574455	40.0	38.0	41.0	31.0	41.0
18-19	37.13185708515543	40.0	38.0	41.0	31.0	41.0
20-21	37.09417675039846	40.0	38.0	41.0	31.0	41.0
22-23	36.979754169536804	40.0	37.5	41.0	30.5	41.0
24-25	36.914920121363	40.0	37.0	41.0	30.0	41.0
26-27	36.77643101566363	40.0	37.0	41.0	30.0	41.0
28-29	36.62142845673098	40.0	37.0	41.0	30.0	41.0
30-31	36.48462767015255	40.0	37.0	41.0	30.0	41.0
32-33	36.43230377639169	40.0	36.5	41.0	30.0	41.0
34-35	36.5587304894252	40.0	37.0	41.0	30.0	41.0
36-37	36.53151480989992	40.0	37.0	41.0	30.0	41.0
38-39	36.440862716454205	40.0	37.0	41.0	30.0	41.0
40-41	36.31942787783166	40.0	37.0	41.0	29.0	41.0
42-43	36.214646688189426	40.0	36.0	41.0	29.0	41.0
44-45	36.0828655681304	40.0	36.0	41.0	28.0	41.0
46-47	35.93665210909164	40.0	36.0	41.0	28.0	41.0
48-49	35.78239726080979	40.0	35.5	41.0	27.0	41.0
50-51	35.629289506070414	40.0	35.0	41.0	27.0	41.0
52-53	35.45942908009768	39.0	35.0	41.0	26.5	41.0
54-55	35.255534643995446	39.0	35.0	41.0	26.0	41.0
56-57	34.993297778648824	39.0	35.0	41.0	26.0	41.0
58-59	34.77713302373611	38.5	35.0	40.5	25.5	41.0
60-61	34.5068026846765	38.0	34.5	40.0	24.5	41.0
62-63	34.21124616932105	37.5	34.0	40.0	24.0	41.0
64-65	33.89747676476801	37.0	34.0	40.0	23.0	41.0
66-67	33.56225741092011	36.5	34.0	39.5	22.5	41.0
68-69	33.19710142719683	36.0	34.0	39.0	22.0	41.0
70-71	32.8124996139985	35.5	33.0	39.0	21.5	40.0
72-73	32.40942181297599	35.0	33.0	37.5	20.0	40.0
74-75	31.99238146254015	35.0	33.0	37.0	18.5	39.0
76-77	29.214112873839888	33.0	28.5	34.5	11.0	36.5
78-79	30.86785470038666	34.5	31.5	36.0	12.5	38.0
80-81	31.05200212290535	35.0	32.0	36.0	14.0	37.0
82-83	30.87742156758197	35.0	32.0	35.0	11.0	37.0
84-85	30.63239645792666	35.0	32.0	35.0	7.5	36.5
86-87	30.376088987449084	35.0	32.0	35.0	4.0	36.0
88-89	30.083317241359435	35.0	31.0	35.0	2.0	36.0
90-91	29.889920399284073	34.5	31.0	35.0	2.0	35.0
92-93	29.738129990484808	34.0	31.0	35.0	2.0	35.0
94-95	29.628763308817263	34.0	31.0	35.0	2.0	35.0
96-97	29.428681744125676	34.0	31.0	35.0	2.0	35.0
98-99	29.150860505437578	34.0	31.0	35.0	2.0	35.0
100-101	28.053327703544255	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48468.0
3	15858.0
4	8620.0
5	5919.0
6	5347.0
7	5398.0
8	5639.0
9	6104.0
10	6657.0
11	7337.0
12	7801.0
13	8563.0
14	9096.0
15	9939.0
16	10659.0
17	11645.0
18	10846.0
19	9801.0
20	9493.0
21	9077.0
22	9977.0
23	10932.0
24	12684.0
25	15224.0
26	18203.0
27	21820.0
28	25892.0
29	31448.0
30	38070.0
31	46951.0
32	59928.0
33	78361.0
34	111436.0
35	167030.0
36	289330.0
37	568708.0
38	625121.0
39	85363.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56643124796653	19.534020249554544	12.224576821587759	26.67497168089117
2	15.490039053252003	21.517497792060475	36.86532289442858	26.12714026025894
3	17.91688901625952	24.842914130234277	29.443697789021527	27.796499064484674
4	12.98454192768445	16.73323357904073	35.69109219421288	34.59113229906195
5	13.75032227189122	37.52159118708903	34.800380878012774	13.927705663006975
6	33.361029780817894	35.80878910664958	16.98468858583833	13.8454925266942
7	28.351197161989898	31.938977512656745	21.570902491164155	18.138922834189195
8	26.40219672442991	34.89741660891989	20.222118188814047	18.47826847783615
9	26.5934656841573	15.059946548570105	19.45859325816975	38.88799450910284
10-11	25.34429238273389	25.311793597059157	28.402764024812832	20.941149995394113
12-13	25.919421264296233	22.93061121135732	27.87133671025232	23.278630814094125
14-15	23.10519981207334	24.561281425936762	25.021771739931577	27.311747022058324
16-17	23.362598152705456	27.425526229433274	25.932427541676944	23.279448076184327
18-19	23.286094789184666	26.927287721972366	27.13457653092945	22.652040957913517
20-21	23.731855191509947	26.269750589300973	27.048739564998098	22.949654654190983
22-23	23.597802318155786	26.143256938471417	26.670320137058667	23.588620606314137
24-25	23.35910773720031	26.28678328728335	26.76354010569022	23.59056886982612
26-27	23.311217130902417	26.479492828841263	26.793407301970156	23.41588273828616
28-29	23.369878513970235	26.647888982587443	26.597221764709754	23.385010738732568
30-31	23.69021950399937	26.385736358005985	26.500606988021662	23.423437149972987
32-33	23.416794066021176	26.432395982931062	26.475922808859785	23.674887142187977
34-35	23.65585221101829	26.4626075140135	26.597590038964984	23.283950236003232
36-37	23.66044132548341	26.32934757233104	26.60467676714858	23.40553433503697
38-39	23.554762553245105	26.399750982452048	26.61998822848595	23.4254982358169
40-41	23.66887160894906	26.204596409094	26.680419247849102	23.446112734107842
42-43	23.440592163615833	26.24092141791825	26.485494445583686	23.83299197288223
44-45	23.38820373800643	26.326491157960923	26.601203480032552	23.684101624000096
46-47	23.531968389029714	26.29527235473069	26.41699237890999	23.755766877329602
48-49	23.31478458310402	26.36366462690123	26.51810371611209	23.803447073882662
50-51	23.40539116076925	26.357373883361024	26.518392245754253	23.718842710115478
52-53	23.558248157871333	26.259062416622474	26.463924088158663	23.718765337347534
54-55	23.106473281009517	26.171439135479485	26.69819765292199	24.02388993058901
56-57	23.187437862850587	26.325728058496267	26.500149571800534	23.98668450685261
58-59	23.200218076149177	26.19355617097648	26.554950680654123	24.051275072220225
60-61	23.307894976859654	26.193834552750705	26.60743517583843	23.890835294551216
62-63	23.224748356091187	26.154185108198902	26.619287500527637	24.001779035182274
64-65	23.007333266471942	26.008064142450177	26.56006188042095	24.42454071065693
66-67	23.0201586097306	26.218693442664815	26.360856495840345	24.40029145176424
68-69	23.042345229919555	26.14873289670532	26.381015432991738	24.42790644038339
70-71	22.96863875261021	25.91357630835941	26.435855289240894	24.681929649789485
72-73	23.000092252746164	26.082569502560016	26.277721126537823	24.639617118156
74-75	22.923051483995767	26.186332779201717	26.188186062199886	24.70242967460263
76-77	22.89168262762871	25.980023018455416	26.30348659227596	24.82480776163991
78-79	22.843479538145324	25.98232291550141	26.297871831969843	24.876325714383423
80-81	22.91090780409472	26.106051780121923	26.116103612974772	24.86693680280858
82-83	22.88788939975262	26.083392481098965	26.1811999264488	24.84751819269961
84-85	22.899284869068396	25.97398131013568	26.12499366748945	25.00174015330648
86-87	22.761688052405397	26.11599848852777	26.07594529855079	25.046368160516042
88-89	22.689184907521987	25.90358672890695	26.119989720651482	25.287238642919586
90-91	22.652357256654458	25.798161151042287	26.197422439178332	25.352059153124923
92-93	22.677557810022506	25.71661853026826	26.24080475324792	25.365018906461316
94-95	22.60867434835743	25.607790723337253	26.25134231946215	25.532192608843168
96-97	22.553864436706714	25.512125983733196	26.24782582198753	25.68618375757256
98-99	22.3755747632238	25.533681871193338	26.149994801783244	25.94074856379962
100-101	22.32327975675921	25.560067105734763	26.126871342314388	25.989781795191636
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	162.0
1	135.5
2	132.0
3	157.5
4	141.0
5	146.0
6	230.0
7	401.5
8	551.5
9	592.0
10	504.0
11	409.0
12	407.0
13	432.5
14	462.5
15	510.0
16	553.5
17	575.0
18	649.5
19	732.5
20	823.0
21	936.5
22	1116.0
23	1372.5
24	1666.0
25	2155.5
26	2958.0
27	4179.0
28	5882.0
29	8572.0
30	12433.5
31	17142.0
32	23351.5
33	28894.5
34	33550.0
35	39027.0
36	46107.0
37	53367.5
38	59372.5
39	65241.0
40	70548.5
41	75687.5
42	80634.0
43	86080.5
44	91468.5
45	96488.0
46	100259.0
47	102889.0
48	106316.5
49	108358.0
50	107154.5
51	105059.0
52	103166.5
53	100538.5
54	96895.0
55	91005.0
56	83480.5
57	74660.0
58	66240.0
59	57732.5
60	47706.5
61	38909.0
62	31485.0
63	23918.5
64	17039.0
65	12294.0
66	9212.0
67	7039.0
68	5350.5
69	4085.5
70	3146.0
71	2324.0
72	1650.5
73	1215.5
74	911.5
75	657.5
76	476.5
77	295.0
78	162.5
79	96.5
80	60.0
81	36.0
82	20.0
83	11.0
84	6.0
85	6.5
86	6.5
87	3.5
88	1.5
89	1.5
90	2.5
91	2.0
92	1.5
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15258896871514407
2	4.940819278418049E-4
3	0.0057231156641675725
4	0.004652604820510329
5	0.027709761453127892
6	0.0014822457835254146
7	1.235204819604512E-4
8	0.0016469397594726827
9	0.0
10-11	0.10303666870200973
12-13	1.8528072294067682E-4
14-15	0.00576428915815439
16-17	2.470409639209024E-4
18-19	4.117349398681707E-4
20-21	9.058168677099755E-4
22-23	2.470409639209024E-4
24-25	0.0018733939764001767
26-27	0.002470409639209024
28-29	0.006381891567956647
30-31	0.014945978317214595
32-33	0.014801871088260737
34-35	0.011713859039249456
36-37	7.411228917627073E-4
38-39	1.0293373496704267E-4
40-41	0.004611431326523512
42-43	0.00942873012298111
44-45	0.008502326508277725
46-47	0.009181689159060206
48-49	0.011590338557289005
50-51	0.005846636146128023
52-53	0.0014410722895385976
54-55	0.0010293373496704267
56-57	0.006073090363055518
58-59	0.009778704821869055
60-61	0.008090591568409555
62-63	0.019125087956876532
64-65	0.03452397470794611
66-67	0.03709731808212218
68-69	0.02756565422417403
70-71	0.04205872410753364
72-73	0.02614516868162884
74-75	0.025671673500780444
76-77	0.24327358922110867
78-79	0.07026256748850333
80-81	0.05471957350847989
82-83	0.020854374704322845
84-85	0.032938795189453654
86-87	0.08127647712997689
88-89	0.26410737717843813
90-91	0.3201650892414895
92-93	0.05229033736325768
94-95	0.004734951808483963
96-97	0.010005159038796547
98-99	0.0012146180726111035
100-101	0.0017498734944397256
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2428747.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.90252077426622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.25796725814534	53.551048753045684
2	11.172343307773067	14.725711738754976
3	3.2165743777793554	6.359410792607294
4	1.3821794651026291	3.643564436507608
5	0.7845821030199256	2.5852969171694062
6	0.4941051591317815	1.953766530861261
7	0.3188968898513971	1.4711276235796407
8	0.24052691528912706	1.2681064025289495
9	0.1822042541956244	1.080694768255814
>10	0.9048747039521599	10.319680340974129
>50	0.035316014836950994	1.5606598428377072
>100	0.009552467017178459	1.0429162489443182
>500	7.517862046359715E-4	0.33161856261690176
>1k	1.2529770077081317E-4	0.10639704131649012
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.117349398681707E-5
2	0.0	0.0	0.0	0.0	4.117349398681707E-5
3	0.0	0.0	0.0	0.0	4.117349398681707E-5
4	0.0	0.0	0.0	4.117349398681707E-5	4.117349398681707E-5
5	0.0	0.0	0.0	4.117349398681707E-5	4.117349398681707E-5
6	0.0	0.0	0.0	4.117349398681707E-5	4.117349398681707E-5
7	0.0	0.0	0.0	4.117349398681707E-5	4.117349398681707E-5
8	0.0	0.0	0.0	4.117349398681707E-5	4.117349398681707E-5
9	0.0	0.0	0.0	1.235204819604512E-4	8.234698797363414E-5
10-11	0.0	0.0	0.0	1.235204819604512E-4	8.234698797363414E-5
12-13	0.0	0.0	0.0	1.235204819604512E-4	8.234698797363414E-5
14-15	0.0	0.0	0.0	1.646939759472683E-4	1.646939759472683E-4
16-17	0.0	0.0	0.0	2.2645421692749388E-4	2.0586746993408536E-4
18-19	0.0	0.0	0.0	2.470409639209024E-4	2.0586746993408536E-4
20-21	0.0	0.0	0.0	2.882144579077195E-4	2.0586746993408536E-4
22-23	0.0	0.0	0.0	4.5290843385498776E-4	2.0586746993408536E-4
24-25	0.0	0.0	0.0	0.0013587253015649634	2.2645421692749388E-4
26-27	0.0	0.0	0.0	0.0026351036151562924	2.470409639209024E-4
28-29	0.0	0.0	0.0	0.009099342171086572	2.470409639209024E-4
30-31	0.0	0.0	0.0	0.03621208796140561	3.08801204901128E-4
32-33	0.0	0.0	0.0	0.08893474701152487	3.2938795189453655E-4
34-35	0.0	0.0	0.0	0.1581885638973512	3.2938795189453655E-4
36-37	0.0	0.0	0.0	0.2577872458514617	3.2938795189453655E-4
38-39	0.0	0.0	0.0	0.40216210251623574	3.2938795189453655E-4
40-41	0.0	0.0	0.0	0.5638915868964531	3.2938795189453655E-4
42-43	0.0	0.0	0.0	0.7372937568219333	3.2938795189453655E-4
44-45	0.0	0.0	0.0	0.9520135279631843	3.2938795189453655E-4
46-47	0.0	0.0	0.0	1.1926931870631234	3.2938795189453655E-4
48-49	0.0	0.0	0.0	1.4319935341145043	3.7056144588135364E-4
50-51	0.0	0.0	0.0	1.678437482372598	4.1173493986817073E-4
52-53	0.0	0.0	0.0	1.9401362101528072	4.1173493986817073E-4
54-55	0.0	4.117349398681707E-5	0.0	2.244614198185319	4.1173493986817073E-4
56-57	0.0	4.117349398681707E-5	0.0	2.592139074181049	4.1173493986817073E-4
58-59	0.0	4.117349398681707E-5	0.0	2.952942401987527	4.1173493986817073E-4
60-61	0.0	4.117349398681707E-5	0.0	3.329453417749976	4.1173493986817073E-4
62-63	0.0	4.117349398681707E-5	0.0	3.712490432309335	4.5290843385498776E-4
64-65	0.0	4.117349398681707E-5	0.0	4.132501244468855	4.734951808483963E-4
66-67	0.0	4.117349398681707E-5	0.0	4.6151987012233056	4.940819278418049E-4
68-69	0.0	4.117349398681707E-5	0.0	5.130958473649169	4.940819278418049E-4
70-71	0.0	4.117349398681707E-5	0.0	5.674510354516135	5.352554218286219E-4
72-73	0.0	4.117349398681707E-5	0.0	6.229508466711436	5.76428915815439E-4
74-75	0.0	4.117349398681707E-5	0.0	6.8162101692765855	5.970156628088475E-4
76-77	0.0	4.117349398681707E-5	0.0	7.482088500778385	6.17602409802256E-4
78-79	0.0	4.117349398681707E-5	0.0	8.1941429057864	6.17602409802256E-4
80-81	0.0	4.117349398681707E-5	0.0	8.927401660197624	6.381891567956645E-4
82-83	0.0	4.117349398681707E-5	0.0	9.681700070036113	6.999493977758902E-4
84-85	0.0	4.117349398681707E-5	0.0	10.463502373857796	6.999493977758902E-4
86-87	0.0	4.117349398681707E-5	0.0	11.310626425889563	6.999493977758902E-4
88-89	0.0	4.117349398681707E-5	0.0	12.218501968298881	6.999493977758902E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1830	0.0	47.26945	1
GTATCAA	3070	0.0	39.01417	1
TCAACGC	3715	0.0	31.045532	4
GTACATG	5600	0.0	30.89396	1
CAACGCA	3865	0.0	30.137154	5
ATCAACG	4020	0.0	28.926218	3
AACGCAG	4105	0.0	28.603859	6
TACATGG	6055	0.0	27.983736	2
TATCAAC	4180	0.0	27.93254	2
ACATGGG	6300	0.0	26.29278	3
CATGGGG	4685	0.0	25.428207	4
ACGCAGA	4985	0.0	23.07667	7
CGCAGAG	5250	0.0	21.821302	8
ATGGGGG	2695	0.0	20.640144	5
GAGTACA	4200	0.0	18.445845	1
AGAGTAC	5100	0.0	17.429878	10-11
GCAGAGT	6130	0.0	17.292881	9
GTACCGT	280	7.08078E-7	16.977856	6
GTGTAGC	1445	0.0	16.117157	1
AGTACAT	4550	0.0	15.855641	2
>>END_MODULE