FastQCFastQC Report
Mon 15 Aug 2016
SRR2921372_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921372_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538435
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.053.0665171
GTATCAA36950.038.7882921
TCAACGC45000.030.6894154
CAACGCA46150.030.1291545
TATCAAC47950.029.297932
ATCAACG48000.028.87023
AACGCAG49650.027.9969716
GTACATG64950.027.6928841
TACATGG67850.026.3709242
ACATGGG70950.024.2138753
ACGCAGA59750.022.6332327
CGCAGAG62750.021.4785128
CATGGGG60000.021.3560374
GTGTACG2801.5952537E-920.3390831
GAGTACA50150.018.73711
GTGGTAT11250.018.5612961
ATGGGGG38050.018.084415
GCAGAGT69800.017.7450059
CAGAGTA62750.017.20532810-11
AGAGTAC57200.017.17392710-11
Produced by FastQC (version 0.11.5)