##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921372_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2538435
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.473061551704102	31.0	31.0	34.0	26.0	34.0
2	30.52954123308259	33.0	31.0	34.0	26.0	34.0
3	30.575432106790206	33.0	31.0	34.0	26.0	34.0
4	34.03444957227583	37.0	35.0	37.0	30.0	37.0
5	34.02944530783731	37.0	35.0	37.0	30.0	37.0
6	34.07694150135812	37.0	35.0	37.0	30.0	37.0
7	33.99143842564415	37.0	35.0	37.0	30.0	37.0
8	33.95966412376129	37.0	35.0	37.0	30.0	37.0
9	35.52980990255807	39.0	37.0	39.0	30.0	39.0
10-11	35.43022098261331	39.0	37.0	39.0	30.0	39.0
12-13	35.31843005631423	39.0	35.0	39.0	29.0	39.0
14-15	36.466890820525244	40.0	37.0	41.0	29.0	41.0
16-17	36.41352762627367	40.0	37.0	41.0	29.0	41.0
18-19	36.36498433089679	40.0	37.0	41.0	28.5	41.0
20-21	36.2974175033042	40.0	37.0	41.0	28.0	41.0
22-23	36.18316659674169	40.0	36.5	41.0	27.0	41.0
24-25	36.08620784065773	40.0	36.0	41.0	27.0	41.0
26-27	35.941674890237486	40.0	36.0	41.0	27.0	41.0
28-29	35.798816987632144	40.0	36.0	41.0	27.0	41.0
30-31	35.64909737692712	40.0	36.0	41.0	26.5	41.0
32-33	35.46510468063984	39.5	35.0	41.0	25.0	41.0
34-35	35.29723491048618	39.0	35.0	41.0	25.0	41.0
36-37	35.12342801765655	39.0	35.0	41.0	24.5	41.0
38-39	35.35400315548753	39.5	35.0	41.0	25.0	41.0
40-41	35.46685182011751	40.0	35.0	41.0	25.0	41.0
42-43	35.28852304668034	40.0	35.0	41.0	24.0	41.0
44-45	35.161844798074405	40.0	35.0	41.0	24.0	41.0
46-47	35.00326342805705	40.0	35.0	41.0	23.0	41.0
48-49	34.84983346038011	39.5	35.0	41.0	22.5	41.0
50-51	33.10962758550052	37.0	32.5	39.5	18.5	40.0
52-53	33.26202739089242	37.5	33.5	39.5	18.5	40.0
54-55	34.124766637711815	39.0	34.0	40.5	19.5	41.0
56-57	34.131645876297796	39.0	34.0	41.0	19.0	41.0
58-59	33.946536350152755	38.0	34.0	41.0	18.0	41.0
60-61	33.69711574257367	38.0	34.0	40.0	17.0	41.0
62-63	33.40268196743269	37.0	34.0	40.0	13.5	41.0
64-65	33.10444013732871	37.0	33.0	40.0	12.5	41.0
66-67	32.76076696862437	36.0	33.0	39.0	8.0	41.0
68-69	32.393214322998226	36.0	33.0	39.0	7.0	41.0
70-71	32.011107434304996	35.0	32.5	39.0	3.5	40.5
72-73	31.65341401296468	35.0	32.0	37.5	2.0	40.0
74-75	31.2556265967023	35.0	32.0	37.0	2.0	39.0
76-77	30.878779641787162	35.0	32.0	36.5	2.0	39.0
78-79	30.528406084851493	35.0	31.5	36.0	2.0	38.5
80-81	30.17345726796235	35.0	31.0	36.0	2.0	37.0
82-83	29.87008688424167	35.0	31.0	35.0	2.0	37.0
84-85	29.628411994004182	35.0	31.0	35.0	2.0	36.5
86-87	29.394276394707763	35.0	31.0	35.0	2.0	36.0
88-89	29.17517545259185	35.0	31.0	35.0	2.0	36.0
90-91	28.905747241902986	35.0	30.0	35.0	2.0	35.5
92-93	28.69927258330428	34.0	30.0	35.0	2.0	35.0
94-95	28.472737730136874	34.0	30.0	35.0	2.0	35.0
96-97	28.20170912392872	34.0	29.5	35.0	2.0	35.0
98-99	27.950362920460833	34.0	29.0	35.0	2.0	35.0
100-101	26.885126071772568	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84804.0
3	14465.0
4	10001.0
5	7731.0
6	7140.0
7	7264.0
8	7631.0
9	8254.0
10	8858.0
11	9524.0
12	9523.0
13	9778.0
14	10657.0
15	11579.0
16	11908.0
17	12659.0
18	11717.0
19	10697.0
20	10681.0
21	11187.0
22	12389.0
23	14174.0
24	16527.0
25	19408.0
26	23041.0
27	27327.0
28	32404.0
29	38309.0
30	45189.0
31	55421.0
32	68789.0
33	89022.0
34	123813.0
35	181225.0
36	302933.0
37	543045.0
38	565428.0
39	103920.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65689745620055	18.327645279874698	12.61041254711415	25.40504471681061
2	16.859479244432215	19.616429760118816	37.813942891739316	25.710148103709646
3	19.653194682675814	22.681671564423368	29.303693497124407	28.36144025577641
4	14.001579770113493	15.151524706768967	35.08615028785083	35.76074523526671
5	14.725926031026468	36.4277429172416	34.03393354939377	14.81239750233816
6	34.815030792083505	33.907974420703	16.386589387665044	14.890405399548461
7	29.559109353693184	31.22931742936652	20.48350181456991	18.728071402370386
8	27.125315760917317	33.96895958378465	19.74591761669117	19.159807038606857
9	26.81462965057152	15.121634651188174	19.21543394170032	38.84830175653999
10-11	26.00597864858427	24.807569513660006	27.47737320097271	21.709078636783012
12-13	26.269176276436678	22.451466138059537	27.260019107563682	24.019338477940103
14-15	23.600896857451847	24.211466186156645	24.33196810472848	27.855668851663022
16-17	23.765423950020747	27.190404828322418	25.508010622246267	23.536160599410564
18-19	23.815688605506544	26.43979485668003	26.783906994759903	22.96060954305352
20-21	24.120853107181002	25.66544452515872	26.84782653668582	23.36587583097446
22-23	24.087116708043276	25.52193052119548	26.48103487892268	23.909917891838568
24-25	23.86921320266582	25.683898932817158	26.538697472177518	23.908190392339502
26-27	23.606687160782126	25.96986805451839	26.594208238445265	23.829236546254215
28-29	23.669546012898447	26.21670848269388	26.458482940144467	23.655262564263204
30-31	24.023694213912204	25.782419679695895	26.359803127393533	23.834082978998364
32-33	23.709501080496498	25.884500144788504	26.202404474063158	24.203594300651844
34-35	24.07104916660954	26.003177072129464	26.23919208442565	23.686581676835345
36-37	23.99726913838795	25.921281692787524	26.359157151788555	23.722292017035976
38-39	23.854458164729834	25.992011040664583	26.437120334340193	23.71641046026539
40-41	24.017206530965037	25.784090656151655	26.44139069710364	23.757312115779673
42-43	23.910521654357332	25.769702750123415	26.23624489238403	24.083530703135224
44-45	23.794421803181248	25.818282352996896	26.36230415175777	24.024991692064084
46-47	23.886031171214732	25.936207058173437	26.143107352716257	24.034654417895574
48-49	23.670883428013447	26.038583543934585	26.24348806983213	24.047044958219843
50-51	23.73639957800062	26.043878147481124	26.362212978651772	23.857509295866482
52-53	24.01936260419448	25.78767643026457	26.3026073955312	23.89035357000975
54-55	23.51133344871663	25.772043521737682	26.524416296357987	24.192206733187703
56-57	23.58169788098428	25.9834424512088	26.293151428979698	24.141708238827228
58-59	23.60877399694876	25.844370893603248	26.42100817282737	24.125846936620622
60-61	23.595693011189457	25.849058018898834	26.57260671746251	23.9826422524492
62-63	23.68469510947149	25.81556669527802	26.62079639564036	23.878941799610125
64-65	23.490959861100393	25.65397347889082	26.53453019836852	24.320536461640273
66-67	23.501090853115223	25.863510642601785	26.35570198938848	24.27969651489451
68-69	23.54702323839235	25.726207161404936	26.514292457912802	24.212477142289913
70-71	23.444557463059848	25.593119172374873	26.527906992804844	24.43441637176043
72-73	23.501007767326712	25.77522309209932	26.39365716335697	24.330111977217
74-75	23.45217918260845	25.95661071773405	26.370885006621403	24.22032509303609
76-77	23.540433045446722	25.632629639744454	26.397803674066683	24.429133640742137
78-79	23.465785416020253	25.690820924215764	26.410873442396195	24.432520217367788
80-81	23.596489912129368	25.746316089508188	26.301458878297318	24.355735120065123
82-83	23.60415259446355	25.74653572132123	26.43202884092683	24.217282843288395
84-85	23.576592760030056	25.79175883531073	26.382282934824637	24.249365469834576
86-87	23.51891173523334	25.934057864011123	26.24687791923928	24.300152481516253
88-89	23.437406230555176	25.747845869090746	26.331763007697383	24.482984892656688
90-91	23.551000079160637	25.65694938595232	26.2938273062346	24.49822322865244
92-93	23.643033010837257	25.53823047256256	26.491899694560523	24.326836822039656
94-95	23.59968639182581	25.41065316148195	26.54597240350734	24.443688043184896
96-97	23.644647356581096	25.242146072256254	26.53839608064731	24.574810490515343
98-99	23.559428438313113	25.328482139684372	26.398679675130595	24.71340974687193
100-101	23.77802926530324	25.206960588543986	26.508250585294217	24.506759560858555
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109.0
1	91.0
2	102.0
3	142.0
4	141.0
5	163.5
6	260.0
7	463.5
8	718.0
9	824.0
10	717.0
11	653.5
12	682.0
13	728.0
14	796.5
15	864.5
16	962.5
17	1068.5
18	1204.5
19	1313.0
20	1405.5
21	1564.5
22	1763.5
23	2016.5
24	2357.5
25	2861.0
26	3559.5
27	4679.5
28	6493.5
29	8975.0
30	12316.0
31	16985.5
32	23733.5
33	28558.0
34	31924.5
35	37018.5
36	43372.0
37	49958.5
38	56251.5
39	62576.0
40	68119.0
41	73085.5
42	78248.0
43	84380.5
44	90389.0
45	97185.0
46	101574.5
47	104153.0
48	108090.5
49	110019.0
50	109260.0
51	108509.0
52	108424.5
53	106662.0
54	103636.5
55	98772.5
56	92163.5
57	83846.5
58	75890.5
59	67269.0
60	55612.5
61	45612.0
62	37827.5
63	29656.0
64	21660.0
65	15886.0
66	12018.0
67	9344.0
68	7464.5
69	5876.0
70	4478.5
71	3287.5
72	2389.5
73	1834.0
74	1451.5
75	1104.0
76	822.5
77	563.5
78	348.0
79	238.5
80	184.5
81	137.0
82	100.0
83	88.5
84	75.5
85	58.0
86	57.0
87	44.5
88	31.0
89	30.5
90	25.5
91	23.0
92	25.0
93	20.0
94	15.5
95	12.0
96	9.5
97	8.0
98	6.0
99	6.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.049085361649993005
2	0.056294527927640454
3	0.04802171416640568
4	0.053458134638074244
5	0.0465641231703786
6	0.017530486303568932
7	0.014497121257782847
8	0.020406273944379116
9	0.02158810448169837
10-11	0.02247447738468781
12-13	0.027438165641428678
14-15	0.04455501125693587
16-17	0.029230608623029546
18-19	0.02361691357076309
20-21	0.026650278616549172
22-23	0.04617017965793885
24-25	0.04150194903552779
26-27	0.0313579035902042
28-29	0.020977492037416758
30-31	0.01366983988165937
32-33	0.009848587810993781
34-35	0.009809193459749807
36-37	0.0016545627522469553
38-39	0.007169771926403472
40-41	0.0038015548950436
42-43	0.20999158930600942
44-45	0.18566557741285478
46-47	0.1909641176551694
48-49	0.18972319559098422
50-51	0.18838378764868907
52-53	0.19269746910990435
54-55	0.19108230070890134
56-57	0.2004778534805894
58-59	0.19854753026963462
60-61	0.20315666936517973
62-63	0.1992763257676482
64-65	0.2006945224124313
66-67	0.19175200468004894
68-69	0.19011713910342395
70-71	0.17481243364513963
72-73	0.1818443253421892
74-75	0.1814700790053714
76-77	0.1799140021312344
78-79	0.2055203304398182
80-81	0.21245373625875785
82-83	0.2114688774776585
84-85	0.20727337907017512
86-87	0.223326577202095
88-89	0.22167201444984802
90-91	0.22102200765432245
92-93	0.22419325292946246
94-95	0.23540094585837337
96-97	0.23542064303399535
98-99	0.2255326608717576
100-101	0.23431760119916406
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2538435.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.35649862655349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.99116260860187	48.466211601045444
2	11.577037156545478	14.206529287902828
3	3.8439228448103115	7.075489402445445
4	1.8042464636331406	4.428089826714832
5	0.9848282205024705	3.021280567932548
6	0.6447174137494538	2.373456186674009
7	0.423599578699424	1.8193410878075893
8	0.3082643198894298	1.5131215455929
9	0.23518948709515008	1.2987363097740057
>10	1.130731866334511	11.824763909851564
>50	0.04178920056364635	1.7152446534070727
>100	0.013160391207456842	1.4700247864997653
>500	8.359918466752846E-4	0.3775598564404705
>1k	5.144565210189269E-4	0.41015097791149235
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.9394351243975124E-5
7	0.0	0.0	0.0	0.0	3.9394351243975124E-5
8	0.0	0.0	0.0	0.0	3.9394351243975124E-5
9	0.0	0.0	0.0	0.0	3.9394351243975124E-5
10-11	0.0	0.0	0.0	0.0	5.909152686596269E-5
12-13	0.0	0.0	0.0	3.9394351243975124E-5	2.5606328308583836E-4
14-15	0.0	0.0	0.0	3.9394351243975124E-5	4.1364068806173883E-4
16-17	0.0	0.0	0.0	3.9394351243975124E-5	5.121265661716766E-4
18-19	0.0	0.0	0.0	3.9394351243975124E-5	6.697039711475772E-4
20-21	0.0	0.0	0.0	3.9394351243975124E-5	6.697039711475772E-4
22-23	0.0	0.0	0.0	1.3788022935391295E-4	6.894011467695647E-4
24-25	0.0	0.0	0.0	4.924293905496891E-4	7.090983223915523E-4
26-27	0.0	0.0	0.0	0.0013197107666731668	7.090983223915523E-4
28-29	0.0	0.0	0.0	0.005613695052266455	7.090983223915523E-4
30-31	0.0	0.0	0.0	0.024385103420020607	7.090983223915523E-4
32-33	0.0	0.0	0.0	0.0619279201555289	7.484926736355274E-4
34-35	0.0	0.0	0.0	0.11434210448563781	7.484926736355274E-4
36-37	0.0	0.0	0.0	0.18732014016510173	7.484926736355274E-4
38-39	0.0	0.0	0.0	0.2887605946183377	7.484926736355274E-4
40-41	0.0	0.0	0.0	0.40054206627311706	7.484926736355274E-4
42-43	0.0	0.0	0.0	0.5282191586548405	7.484926736355274E-4
44-45	0.0	0.0	0.0	0.700077015956682	7.68189849257515E-4
46-47	0.0	0.0	0.0	0.9007124468422473	7.878870248795026E-4
48-49	0.0	0.0	0.0	1.0847234615028551	7.878870248795026E-4
50-51	0.0	0.0	0.0	1.2647162523365774	7.878870248795026E-4
52-53	0.0	0.0	0.0	1.4531591315121324	8.075842005014901E-4
54-55	0.0	0.0	0.0	1.697975327317816	8.272813761234777E-4
56-57	0.0	0.0	0.0	1.9879965411759608	9.060700786114278E-4
58-59	0.0	0.0	0.0	2.280066261298792	9.45464429855403E-4
60-61	0.0	0.0	0.0	2.56268133712307	9.45464429855403E-4
62-63	0.0	0.0	0.0	2.855558641446403	9.45464429855403E-4
64-65	0.0	0.0	0.0	3.191651549084377	9.651616054773906E-4
66-67	0.0	0.0	0.0	3.61098472090087	9.848587810993782E-4
68-69	0.0	0.0	0.0	4.041761951753738	9.848587810993782E-4
70-71	0.0	0.0	0.0	4.460996637692121	0.0010045559567213656
72-73	0.0	0.0	0.0	4.885608652575307	0.001260619239807204
74-75	0.0	0.0	0.0	5.360842408807001	0.001260619239807204
76-77	0.0	0.0	0.0	5.936925704223271	0.001260619239807204
78-79	0.0	0.0	0.0	6.567570176112447	0.0012803164154291916
80-81	0.0	0.0	0.0	7.170835573887061	0.0013197107666731668
82-83	0.0	0.0	0.0	7.783319249852764	0.0014575909960270798
84-85	0.0	0.0	0.0	8.436930628517176	0.0014575909960270798
86-87	1.9697175621987562E-5	0.0	0.0	9.180577796949695	0.001496985347271055
88-89	3.9394351243975124E-5	0.0	0.0	9.987078652791975	0.00153637969851503
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2200	0.0	53.066517	1
GTATCAA	3695	0.0	38.788292	1
TCAACGC	4500	0.0	30.689415	4
CAACGCA	4615	0.0	30.129154	5
TATCAAC	4795	0.0	29.29793	2
ATCAACG	4800	0.0	28.8702	3
AACGCAG	4965	0.0	27.996971	6
GTACATG	6495	0.0	27.692884	1
TACATGG	6785	0.0	26.370924	2
ACATGGG	7095	0.0	24.213875	3
ACGCAGA	5975	0.0	22.633232	7
CGCAGAG	6275	0.0	21.478512	8
CATGGGG	6000	0.0	21.356037	4
GTGTACG	280	1.5952537E-9	20.339083	1
GAGTACA	5015	0.0	18.7371	1
GTGGTAT	1125	0.0	18.561296	1
ATGGGGG	3805	0.0	18.08441	5
GCAGAGT	6980	0.0	17.745005	9
CAGAGTA	6275	0.0	17.205328	10-11
AGAGTAC	5720	0.0	17.173927	10-11
>>END_MODULE