FastQCFastQC Report
Mon 15 Aug 2016
SRR2921372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538435
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20600.055.3667261
GTATCAA36950.040.384991
TCAACGC47200.031.1685454
CAACGCA48500.030.0857935
TATCAAC49300.029.8408782
ATCAACG50050.029.2988933
AACGCAG51350.028.4137446
GTACATG59600.027.5091821
ACCGTAT1606.2162144E-826.7328038
TACATGG62600.026.0025882
ACATGGG66650.023.7817133
ACGCAGA62350.022.9430947
CGCAGAG65200.021.6486518
CATGGGG57150.020.7596764
GCAGAGT73050.018.0211099
CTTAGCG2905.2750693E-818.0007863
AGAGTAC60000.017.7426610-11
TAAGGTG11750.017.3938395
ATGGGGG34800.017.3455985
TACCGTA2202.9062829E-517.281817
Produced by FastQC (version 0.11.5)