##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921372_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2538435
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.355120773232326	33.0	31.0	34.0	28.0	34.0
2	31.480520478168636	34.0	31.0	34.0	28.0	34.0
3	31.55335984573172	34.0	31.0	34.0	28.0	34.0
4	35.039009862375835	37.0	35.0	37.0	32.0	37.0
5	34.76224563559831	37.0	35.0	37.0	32.0	37.0
6	34.71806447673468	37.0	35.0	37.0	32.0	37.0
7	34.57571810978024	37.0	35.0	37.0	32.0	37.0
8	34.53783925922862	37.0	35.0	37.0	32.0	37.0
9	36.15522556220664	39.0	37.0	39.0	32.0	39.0
10-11	36.007344682845925	39.0	37.0	39.0	31.0	39.0
12-13	35.93730507182575	39.0	37.0	39.0	31.0	39.0
14-15	37.17752473472829	40.0	38.0	41.0	31.0	41.0
16-17	37.14909914967293	40.0	38.0	41.0	31.0	41.0
18-19	37.11099181188409	40.0	38.0	41.0	31.0	41.0
20-21	37.0703760387798	40.0	38.0	41.0	31.0	41.0
22-23	36.959105905804165	40.0	37.5	41.0	30.5	41.0
24-25	36.886685694138315	40.0	37.0	41.0	30.0	41.0
26-27	36.74840994549791	40.0	37.0	41.0	30.0	41.0
28-29	36.59221764591175	40.0	37.0	41.0	30.0	41.0
30-31	36.44712076535346	40.0	37.0	41.0	30.0	41.0
32-33	36.387035319005605	40.0	36.5	41.0	29.5	41.0
34-35	36.51319159245756	40.0	37.0	41.0	30.0	41.0
36-37	36.48171353609606	40.0	37.0	41.0	30.0	41.0
38-39	36.38676822530417	40.0	37.0	41.0	29.5	41.0
40-41	36.254029549702864	40.0	36.5	41.0	29.0	41.0
42-43	36.139251546720715	40.0	36.0	41.0	28.5	41.0
44-45	35.99096746617502	40.0	36.0	41.0	28.0	41.0
46-47	35.825423735490574	40.0	35.5	41.0	27.5	41.0
48-49	35.654902134582926	40.0	35.0	41.0	27.0	41.0
50-51	35.488216558627656	39.5	35.0	41.0	26.0	41.0
52-53	35.30494891537502	39.0	35.0	41.0	26.0	41.0
54-55	35.088127133450335	39.0	35.0	41.0	26.0	41.0
56-57	34.81887087910465	39.0	35.0	41.0	25.0	41.0
58-59	34.58938716177487	38.0	34.5	40.0	24.5	41.0
60-61	34.3094054013595	38.0	34.0	40.0	23.5	41.0
62-63	34.004839005135054	37.0	34.0	40.0	23.0	41.0
64-65	33.68552119711555	37.0	34.0	40.0	22.5	41.0
66-67	33.34443466151389	36.0	34.0	39.0	22.0	41.0
68-69	32.983016110319944	36.0	33.5	39.0	21.5	41.0
70-71	32.60116193638994	35.0	33.0	38.5	20.0	40.0
72-73	32.20891710837583	35.0	33.0	37.5	19.5	39.5
74-75	31.817634487390855	35.0	33.0	37.0	18.0	39.0
76-77	29.08185358301473	33.0	28.5	34.5	10.0	36.5
78-79	30.7330900732144	34.5	31.5	36.0	11.5	37.0
80-81	30.93816111107828	35.0	32.0	35.5	12.0	37.0
82-83	30.790997011938458	35.0	32.0	35.0	9.5	37.0
84-85	30.566516968131943	35.0	32.0	35.0	7.0	36.0
86-87	30.329706689357813	35.0	32.0	35.0	2.0	36.0
88-89	30.054117202134385	35.0	31.5	35.0	2.0	36.0
90-91	29.880792890107486	34.5	31.0	35.0	2.0	35.0
92-93	29.744399403569524	34.0	31.0	35.0	2.0	35.0
94-95	29.65345419520295	34.0	31.0	35.0	2.0	35.0
96-97	29.466138782359998	34.0	31.0	35.0	2.0	35.0
98-99	29.20182671606718	34.0	31.0	35.0	2.0	35.0
100-101	28.123639368350972	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51349.0
3	17670.0
4	9248.0
5	6602.0
6	5792.0
7	5840.0
8	5966.0
9	6498.0
10	6875.0
11	7666.0
12	8276.0
13	8795.0
14	9686.0
15	10560.0
16	11706.0
17	12632.0
18	11622.0
19	10155.0
20	9711.0
21	9634.0
22	10039.0
23	11188.0
24	12940.0
25	15650.0
26	18941.0
27	22697.0
28	27341.0
29	32369.0
30	39776.0
31	49260.0
32	62392.0
33	83274.0
34	118898.0
35	179170.0
36	315961.0
37	606668.0
38	625750.0
39	79837.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.41667873257824	19.513715489355892	12.976266516326065	26.0933392617398
2	16.059411806881293	20.953444231801065	36.35630538390446	26.630838577413172
3	18.462265786280476	23.74580424539452	29.03068219424177	28.761247774083238
4	13.682254816872755	16.229415002145124	34.15676607768061	35.93156410330152
5	14.543707844403611	36.863294599867366	33.93332180090323	14.659675754825791
6	34.83016684112019	34.53226300158013	16.09019529207931	14.547374865220378
7	29.507984250138374	31.52993872208664	20.361561355717203	18.60051567205778
8	27.190617374607722	34.5191881165808	19.3697274733552	18.920467035456277
9	26.739280989775587	15.187670823822877	19.104889077281506	38.96815910912003
10-11	26.055354827897254	25.136868878559493	27.34780872762763	21.459967565915623
12-13	26.3394945575601	22.61262327750288	27.234595248755184	23.813286916181838
14-15	23.522380650810238	24.436260918982114	24.300027873013306	27.74133055719434
16-17	23.72894242597041	27.448843821617913	25.431748592386427	23.39046516002525
18-19	23.779177816143687	26.6253943147762	26.795165107463394	22.800262761616715
20-21	24.090160233909835	25.77859639916893	26.84044052703775	23.290802839883487
22-23	24.054689795960176	25.592532537879777	26.537113943837635	23.815663722322416
24-25	23.84847784097154	25.86376226608798	26.522188574618433	23.765571318322046
26-27	23.58294778377288	26.13155381725581	26.623326483788006	23.6621719151833
28-29	23.672994856343212	26.34508831204851	26.49195988451986	23.489956947088412
30-31	23.997045765807794	25.957899995744764	26.387698124388308	23.657356114059127
32-33	23.759845079134934	25.98541411387393	26.221262612930296	24.03347819406084
34-35	24.05363322889941	26.07252274116987	26.323278416218564	23.550565613712156
36-37	23.99472821261116	26.039229945580278	26.413281815682048	23.55276002612651
38-39	23.872093405748156	26.087537161249834	26.49828309264327	23.542086340358736
40-41	23.989838900558954	25.945038978721257	26.418582772461026	23.64653934825876
42-43	23.897965262188293	25.929137574688344	26.177816370093304	23.99508079303006
44-45	23.776442805846944	26.008051330581267	26.303318002343378	23.912187861228407
46-47	23.92737196750366	26.045571387681832	26.1414269637047	23.885629681109812
48-49	23.670125481009222	26.185911566402815	26.23655863141426	23.907404321173704
50-51	23.80325692347392	26.179979789503545	26.219731038870737	23.797032248151805
52-53	24.054838707771207	25.951828905690004	26.15638774112484	23.83694464541395
54-55	23.482242469319452	25.93528799062092	26.3698503318605	24.21261920819913
56-57	23.601072303251676	26.08778128606658	26.126961445659248	24.1841849650225
58-59	23.56514584092978	26.026128425590876	26.288933448139396	24.119792285339948
60-61	23.58380327801683	26.03597292929516	26.426380936096717	23.953842856591294
62-63	23.598988880721848	25.967565931876123	26.407108489252966	24.02633669814906
64-65	23.372007997523582	25.770437497327624	26.397443503746054	24.46011100140274
66-67	23.331693914129115	26.057780068761026	26.147120533567897	24.463405483541965
68-69	23.43784971896387	25.87886838430233	26.26621907893285	24.417062817800954
70-71	23.27755386686903	25.6807168599758	26.355576215833608	24.686153057321565
72-73	23.208804251284786	25.929535096505834	26.175124341253397	24.68653631095598
74-75	23.10946944390209	25.96913191920796	26.14394872408163	24.777449912808322
76-77	23.17905625191888	25.629417124647446	26.297636335456154	24.893890287977523
78-79	23.010432149635427	25.689082244018913	26.28824048956026	25.012245116785397
80-81	23.013403384340258	25.799066104823847	26.182332459666576	25.00519805116932
82-83	22.92222322479419	25.752142406548362	26.337484637992496	24.98814973066495
84-85	22.886948948659015	25.705742746323534	26.299514204900902	25.10779410011655
86-87	22.769487103616	25.904838467711777	26.12972852135381	25.195945907318407
88-89	22.618847067564428	25.687643072793303	26.202354575041092	25.491155284601174
90-91	22.528778740455746	25.46169384996385	26.31672234663244	25.692805062947965
92-93	22.51104202156298	25.348228665032796	26.526766900816117	25.613962412588105
94-95	22.32636821957226	25.175145578743713	26.64578537668857	25.852700824995463
96-97	22.248693409219445	25.07537360784134	26.5918088464238	26.08412413651542
98-99	22.119798149354427	25.139009179559736	26.46292770104453	26.278264970041302
100-101	21.983652649530626	25.048958140051596	26.548745604010097	26.418643606407677
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	75.0
2	88.0
3	117.0
4	109.5
5	122.0
6	213.5
7	412.5
8	647.5
9	727.0
10	589.5
11	488.0
12	497.0
13	529.0
14	571.0
15	593.0
16	648.5
17	715.0
18	781.5
19	892.0
20	979.0
21	1132.0
22	1386.0
23	1632.0
24	1953.0
25	2467.0
26	3265.5
27	4575.5
28	6567.5
29	9256.0
30	12911.5
31	17625.0
32	24408.0
33	29204.0
34	32143.5
35	37031.0
36	43238.0
37	49578.0
38	55914.0
39	62760.0
40	68345.5
41	72964.5
42	78358.0
43	84686.5
44	90655.5
45	97179.5
46	101734.0
47	105345.5
48	108985.5
49	110807.0
50	111057.5
51	110200.5
52	109618.0
53	108107.0
54	104746.0
55	99421.5
56	92684.5
57	83937.0
58	75615.0
59	66757.0
60	55198.5
61	45251.5
62	37484.5
63	28856.0
64	20617.0
65	15183.0
66	11680.0
67	9138.0
68	7116.0
69	5416.5
70	4038.0
71	2999.5
72	2147.5
73	1533.0
74	1183.0
75	883.0
76	620.5
77	386.0
78	235.5
79	144.0
80	87.5
81	50.5
82	29.5
83	19.5
84	10.0
85	8.0
86	8.0
87	6.0
88	4.5
89	5.0
90	2.5
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1475712397599308
2	6.697039711475772E-4
3	0.006106124442816144
4	0.004648533446789065
5	0.026157849225999487
6	0.001260619239807204
7	0.0
8	0.0016151684010029801
9	3.9394351243975124E-5
10-11	0.09937225101292727
12-13	2.1666893184186321E-4
14-15	0.00541672329604658
16-17	2.9545763432981345E-4
18-19	4.92429390549689E-4
20-21	8.272813761234777E-4
22-23	3.5454916119577614E-4
24-25	0.0013394079422951544
26-27	0.0026394215333463335
28-29	0.006421279252767946
30-31	0.015088036526442474
32-33	0.014378938204050921
34-35	0.012094065831900364
36-37	6.697039711475772E-4
38-39	1.7727458059788807E-4
40-41	0.005003082607984841
42-43	0.008706151624918503
44-45	0.008706151624918503
46-47	0.009513735825419993
48-49	0.011109207050800986
50-51	0.0059091526865962685
52-53	0.001792442981600868
54-55	7.484926736355274E-4
56-57	0.006322793374658008
58-59	0.009513735825419993
60-61	0.0076031097900872
62-63	0.017944126991630668
64-65	0.035435218943955626
66-67	0.03746402803302035
68-69	0.02690634189963501
70-71	0.04299893438279885
72-73	0.02603966617226756
74-75	0.025035110215546195
76-77	0.23764642387927992
78-79	0.06734464345157548
80-81	0.05619604204953051
82-83	0.020406273944379116
84-85	0.03318974092304904
86-87	0.08211752516806615
88-89	0.2649467092913547
90-91	0.3171836190408657
92-93	0.05156720577836344
94-95	0.004648533446789065
96-97	0.009671313230395894
98-99	9.257672542334155E-4
100-101	0.0016545627522469553
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2538435.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.73866857458068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.37705496690559	47.605179654865715
2	11.994696893112119	14.570838384865791
3	3.9620844048925914	7.2195519459985755
4	1.8352727754467353	4.458880994072404
5	0.9964057416837616	3.0260179054969627
6	0.6241126693756437	2.274466354704244
7	0.4403865897579064	1.8723946583996711
8	0.31030087587678506	1.5077809646625715
9	0.24298126982819113	1.3282522936132737
>10	1.1589056525755774	12.126022351046927
>50	0.043367526972240764	1.7672714354576544
>100	0.01299808612103822	1.4199814585039305
>500	9.116211057390632E-4	0.3981545398333625
>1k	5.209263461296719E-4	0.4252070584788336
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.9394351243975124E-5	3.9394351243975124E-5
5	0.0	0.0	0.0	3.9394351243975124E-5	7.878870248795025E-5
6	0.0	0.0	0.0	3.9394351243975124E-5	1.575774049759005E-4
7	0.0	0.0	0.0	3.9394351243975124E-5	1.575774049759005E-4
8	0.0	0.0	0.0	3.9394351243975124E-5	2.3636610746385076E-4
9	0.0	0.0	0.0	3.9394351243975124E-5	2.3636610746385076E-4
10-11	0.0	0.0	0.0	3.9394351243975124E-5	2.3636610746385076E-4
12-13	0.0	0.0	0.0	7.878870248795025E-5	2.3636610746385076E-4
14-15	0.0	0.0	0.0	7.878870248795025E-5	2.5606328308583836E-4
16-17	0.0	0.0	0.0	7.878870248795025E-5	3.15154809951801E-4
18-19	0.0	0.0	0.0	7.878870248795025E-5	3.15154809951801E-4
20-21	0.0	0.0	0.0	7.878870248795025E-5	3.3485198557378854E-4
22-23	0.0	0.0	0.0	1.3788022935391295E-4	4.1364068806173883E-4
24-25	0.0	3.9394351243975124E-5	0.0	4.924293905496891E-4	5.515209174156518E-4
26-27	0.0	7.878870248795025E-5	0.0	0.001359105117917142	5.909152686596269E-4
28-29	0.0	7.878870248795025E-5	0.0	0.0056924837547544065	5.909152686596269E-4
30-31	0.0	7.878870248795025E-5	0.0	0.024621469527484455	6.303096199036021E-4
32-33	0.0	7.878870248795025E-5	0.0	0.06312944786847013	6.697039711475772E-4
34-35	0.0	7.878870248795025E-5	0.0	0.11629212487221458	7.090983223915523E-4
36-37	0.0	7.878870248795025E-5	0.0	0.18952622383476433	7.090983223915523E-4
38-39	0.0	7.878870248795025E-5	0.0	0.29082879805864637	7.484926736355274E-4
40-41	0.0	7.878870248795025E-5	0.0	0.40231481207909603	7.484926736355274E-4
42-43	0.0	1.1818305373192538E-4	0.0	0.5291252287334519	7.484926736355274E-4
44-45	0.0	1.1818305373192538E-4	0.0	0.6996042837417542	7.68189849257515E-4
46-47	0.0	1.1818305373192538E-4	0.0	0.8988018208069145	7.878870248795026E-4
48-49	0.0	1.1818305373192538E-4	0.0	1.0827340467650344	8.272813761234777E-4
50-51	0.0	1.1818305373192538E-4	0.0	1.2616040985883035	8.272813761234777E-4
52-53	0.0	1.1818305373192538E-4	0.0	1.4501848579932122	8.469785517454653E-4
54-55	0.0	1.1818305373192538E-4	0.0	1.6950010537988958	8.666757273674527E-4
56-57	0.0	1.1818305373192538E-4	0.0	1.983052550094842	8.863729029894403E-4
58-59	0.0	1.1818305373192538E-4	0.0	2.274255594490306	9.060700786114279E-4
60-61	0.0	1.1818305373192538E-4	0.0	2.5563191493971678	9.060700786114279E-4
62-63	0.0	1.1818305373192538E-4	0.0	2.848940390437415	9.45464429855403E-4
64-65	0.0	1.1818305373192538E-4	0.0	3.1852499670072305	9.45464429855403E-4
66-67	0.0	1.1818305373192538E-4	0.0	3.604563441648102	9.651616054773906E-4
68-69	0.0	1.1818305373192538E-4	0.0	4.0346315741785785	9.848587810993782E-4
70-71	0.0	1.1818305373192538E-4	0.0	4.456801139284638	9.848587810993782E-4
72-73	0.0	1.1818305373192538E-4	0.0	4.8833828717300225	0.0010439503079653408
74-75	0.0	1.1818305373192538E-4	0.0	5.3611378664413305	0.0010636474835873284
76-77	0.0	1.1818305373192538E-4	0.0	5.940746956293937	0.0010636474835873284
78-79	0.0	1.1818305373192538E-4	0.0	6.574168729945813	0.0010636474835873284
80-81	0.0	1.1818305373192538E-4	0.0	7.182023569640349	0.001083344659209316
82-83	0.0	1.1818305373192538E-4	0.0	7.798820927067268	0.0011621333616972662
84-85	0.0	1.1818305373192538E-4	0.0	8.458518732998876	0.0012015277129412414
86-87	0.0	1.1818305373192538E-4	0.0	9.20935537053342	0.0012606192398072042
88-89	0.0	1.1818305373192538E-4	0.0	10.023912371205093	0.0013000135910511792
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2060	0.0	55.366726	1
GTATCAA	3695	0.0	40.38499	1
TCAACGC	4720	0.0	31.168545	4
CAACGCA	4850	0.0	30.085793	5
TATCAAC	4930	0.0	29.840878	2
ATCAACG	5005	0.0	29.298893	3
AACGCAG	5135	0.0	28.413744	6
GTACATG	5960	0.0	27.509182	1
ACCGTAT	160	6.2162144E-8	26.732803	8
TACATGG	6260	0.0	26.002588	2
ACATGGG	6665	0.0	23.781713	3
ACGCAGA	6235	0.0	22.943094	7
CGCAGAG	6520	0.0	21.648651	8
CATGGGG	5715	0.0	20.759676	4
GCAGAGT	7305	0.0	18.021109	9
CTTAGCG	290	5.2750693E-8	18.000786	3
AGAGTAC	6000	0.0	17.74266	10-11
TAAGGTG	1175	0.0	17.393839	5
ATGGGGG	3480	0.0	17.345598	5
TACCGTA	220	2.9062829E-5	17.28181	7
>>END_MODULE