FastQCFastQC Report
Mon 15 Aug 2016
SRR2921371_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921371_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3671808
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35600.048.251161
GTATCAA61600.043.2146871
TCAACGC74300.035.1932454
CAACGCA75900.034.198465
ATCAACG78050.033.257323
TATCAAC79650.032.8275682
AACGCAG81100.032.1729166
GTACATG152500.031.9869231
TACATGG158300.030.18712
ACATGGG164450.028.5387733
CATGGGG134050.026.7286284
ACGCAGA96750.026.6785137
ATGGGGG92800.026.3853425
CGCAGAG102150.024.8074258
GAGTACA109850.021.4686891
GCAGAGT117600.020.7409529
TGGGGGG97900.019.9647146
AGTACAT111650.019.9336552
GTATAGG13500.019.332061
GTATAGT9900.018.6929821
Produced by FastQC (version 0.11.5)