##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921371_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3671808
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.465716616990868	31.0	31.0	34.0	26.0	34.0
2	30.504237149654884	31.0	31.0	34.0	26.0	34.0
3	30.555744472477866	33.0	31.0	34.0	26.0	34.0
4	34.02608769303841	37.0	35.0	37.0	30.0	37.0
5	34.023789914941084	37.0	35.0	37.0	30.0	37.0
6	34.07449545292128	37.0	35.0	37.0	30.0	37.0
7	33.99288715531967	37.0	35.0	37.0	30.0	37.0
8	33.95837663625113	37.0	35.0	37.0	30.0	37.0
9	35.51765479022868	39.0	37.0	39.0	30.0	39.0
10-11	35.41044330204629	39.0	37.0	39.0	30.0	39.0
12-13	35.28015585237572	39.0	35.0	39.0	29.0	39.0
14-15	36.40800050002615	40.0	37.0	41.0	29.0	41.0
16-17	36.3507996605487	40.0	37.0	41.0	28.5	41.0
18-19	36.29872150177787	40.0	36.5	41.0	28.0	41.0
20-21	36.235161942018756	40.0	36.5	41.0	27.0	41.0
22-23	36.128784511608444	40.0	36.0	41.0	27.0	41.0
24-25	36.02418181996444	40.0	36.0	41.0	27.0	41.0
26-27	35.876264363496134	40.0	36.0	41.0	27.0	41.0
28-29	35.73954588584152	40.0	36.0	41.0	27.0	41.0
30-31	35.59390469218434	40.0	35.5	41.0	26.0	41.0
32-33	35.41897629178868	39.5	35.0	41.0	25.0	41.0
34-35	35.268605684175206	39.0	35.0	41.0	25.0	41.0
36-37	35.11112046163634	39.0	35.0	41.0	24.5	41.0
38-39	35.358624552264175	39.5	35.0	41.0	25.0	41.0
40-41	35.487422544969675	40.0	35.0	41.0	25.0	41.0
42-43	35.333662326570455	40.0	35.0	41.0	24.5	41.0
44-45	35.23012327986649	40.0	35.0	41.0	24.0	41.0
46-47	35.086493084605735	40.0	35.0	41.0	23.5	41.0
48-49	34.951174189935855	39.5	35.0	41.0	23.0	41.0
50-51	33.23662130481768	37.0	32.5	39.5	19.0	40.0
52-53	33.39650684349509	37.5	33.5	39.5	20.5	40.0
54-55	34.26392474769923	39.0	34.0	40.5	21.5	41.0
56-57	34.28464315127589	39.0	34.0	41.0	21.0	41.0
58-59	34.102819237825074	38.0	34.0	41.0	20.5	41.0
60-61	33.851054303492994	38.0	34.0	40.0	19.5	41.0
62-63	33.54927300120268	37.0	34.0	40.0	18.0	41.0
64-65	33.261007111482954	37.0	33.5	40.0	17.5	41.0
66-67	32.92423746012864	36.0	33.0	39.0	14.0	41.0
68-69	32.55506891972565	36.0	33.0	39.0	12.0	41.0
70-71	32.17749008118072	35.0	33.0	39.0	8.0	40.5
72-73	31.818596451666316	35.0	32.0	37.5	7.0	40.0
74-75	31.421585496845154	35.0	32.0	37.0	2.0	39.0
76-77	31.054963794403193	35.0	32.0	36.5	2.0	39.0
78-79	30.72426513042076	35.0	32.0	36.0	2.0	38.0
80-81	30.38611564112285	35.0	31.5	35.5	2.0	37.0
82-83	30.09300499917207	35.0	31.0	35.0	2.0	37.0
84-85	29.868788618577007	35.0	31.0	35.0	2.0	36.0
86-87	29.654390017125078	35.0	31.0	35.0	2.0	36.0
88-89	29.464883784773058	35.0	31.0	35.0	2.0	36.0
90-91	29.22010682475772	35.0	31.0	35.0	2.0	35.5
92-93	29.030954369073765	34.5	30.5	35.0	2.0	35.0
94-95	28.823054609609216	34.0	30.0	35.0	2.0	35.0
96-97	28.567478065301888	34.0	30.0	35.0	2.0	35.0
98-99	28.354331707976016	34.0	30.0	35.0	2.0	35.0
100-101	27.313141781923235	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	121601.0
3	21432.0
4	14994.0
5	11102.0
6	10268.0
7	10305.0
8	10866.0
9	11615.0
10	12494.0
11	13173.0
12	13192.0
13	13274.0
14	14227.0
15	15271.0
16	15049.0
17	15703.0
18	14967.0
19	14103.0
20	14511.0
21	15149.0
22	16999.0
23	19607.0
24	22800.0
25	27180.0
26	32303.0
27	38530.0
28	45580.0
29	54066.0
30	65640.0
31	79385.0
32	100089.0
33	130060.0
34	190907.0
35	265779.0
36	432771.0
37	794038.0
38	830867.0
39	141899.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.29409552791254	17.30305801364809	12.376524674356478	25.026321784082896
2	16.28057930952544	19.17467440612653	39.54890877376468	24.995837510583353
3	19.8320377352424	23.735099077899648	28.444974388220572	27.98788879863738
4	13.724370814079029	14.74833925654719	35.73894295566282	35.78834697371096
5	14.431504833734781	37.87171256989025	33.439036086800144	14.257746509574828
6	34.48849294001031	33.82377496505893	17.125960283625353	14.561771811305407
7	30.083932762268773	31.671366218574924	20.07868117644239	18.166019842713915
8	27.741625472651776	34.66358198473839	19.383428633224945	18.211363909384882
9	28.3021247582097	15.36978627113256	18.508286314580584	37.81980265607715
10-11	26.680390421483807	24.975095389357744	26.8863284651868	21.458185723971653
12-13	26.862398722234644	22.119392222396115	27.086165664632635	23.93204339073661
14-15	24.13855441547143	23.792060753142135	24.211329800552942	27.858055030833494
16-17	24.372700293825712	26.8199765522425	25.11489932406874	23.69242382986305
18-19	24.43813456603423	26.55975740428909	26.251296151541688	22.75081187813499
20-21	24.719498577572363	25.6148938239426	26.408173394787077	23.257434203697958
22-23	24.723540168111555	25.155767080837578	26.019716827749278	24.10097592330159
24-25	24.484671154185943	25.378396887299477	25.913863987868957	24.223067970645623
26-27	24.04936301372755	25.767966647083572	26.07053972373265	24.112130615456223
28-29	24.064665888234565	26.39217151366181	25.657260059745774	23.885902538357847
30-31	25.00335367693053	25.646565078969047	25.63257837355726	23.717502870543168
32-33	24.529257242046995	25.695074586843347	25.615542161241915	24.160126009867742
34-35	24.652379908292737	25.965765709328565	25.834905140046278	23.54694924233242
36-37	24.58505492338669	25.98401961353044	25.91334488640433	23.517580576678537
38-39	24.606365802400944	26.0300945974949	25.688972195984483	23.67456740411967
40-41	24.668520042950394	25.6799242308182	25.720723021016962	23.930832705214442
42-43	24.27576739460755	25.741542437745668	25.533483301845404	24.44920686580138
44-45	24.00809867383602	25.8230561729878	25.973941107672772	24.194904045503403
46-47	24.306600503685274	25.479601346708	25.671767961507193	24.54203018809953
48-49	23.998701175558907	25.851599757425436	25.868790080910486	24.28090898610517
50-51	24.28370333160797	25.6110558273057	26.04900452314603	24.056236317940304
52-53	24.46555845403246	25.55849496283522	25.962150318165445	24.013796264966874
54-55	23.586611451563016	25.392035103511944	26.45609871266476	24.565254732260275
56-57	23.939288751204828	25.534858373530607	25.906580781502598	24.619272093761975
58-59	24.187415507858827	25.599542862382258	26.137700154616866	24.07534147514205
60-61	24.56195050225564	25.613714121039667	26.12917803856544	23.695157338139254
62-63	24.343837392836328	25.474036209151187	26.293564066794595	23.888562331217894
64-65	23.9213972929203	25.122334901608806	26.42638545137393	24.529882354096962
66-67	24.052918598389518	25.48940958145448	26.047789530584016	24.40988228957199
68-69	23.9061892880687	25.48409920411433	26.231619974416404	24.378091533400568
70-71	23.95470996403708	25.272177270844825	26.187837646864963	24.585275118253133
72-73	23.830932516055505	25.92646654982824	25.778901992551244	24.463698941565006
74-75	23.76332792615498	25.764725125399384	26.10972775077523	24.362219197670406
76-77	23.72032384663162	25.19907558917821	26.12281797152639	24.957782592663783
78-79	23.65547513063251	25.294429721252733	26.371760727257442	24.67833442085732
80-81	24.064204180878264	25.600367033873393	25.8120911023489	24.523337682899438
82-83	24.05356268286662	25.69887611869237	25.994382981533924	24.253178216907088
84-85	24.01478462692451	25.7923839822019	25.768094991241636	24.424736399631954
86-87	23.54763404399928	26.10856995850373	25.8740727143445	24.46972328315249
88-89	23.5376171730261	25.617413223552177	26.121773284993594	24.723196318428126
90-91	24.03163225895381	25.272643650552112	26.196895731460163	24.49882835903392
92-93	24.223183803042286	25.169498522939982	26.10107335075279	24.50624432326494
94-95	24.04485428486099	25.291023519384915	26.027775336012667	24.63634685974143
96-97	23.695930059955433	25.157859894694095	26.265626119685653	24.880583925664816
98-99	23.369235287823432	25.082585049908612	26.244876478642258	25.303303183625697
100-101	23.710333100336854	25.26219610987333	26.25458093324439	24.77288985654542
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	94.0
1	77.0
2	99.5
3	144.5
4	153.0
5	180.5
6	286.5
7	550.0
8	788.5
9	870.5
10	770.0
11	651.0
12	679.5
13	774.0
14	874.0
15	949.5
16	1037.5
17	1099.0
18	1211.0
19	1356.5
20	1440.5
21	1589.0
22	1771.0
23	1828.0
24	1962.5
25	2352.0
26	2975.5
27	3915.5
28	5381.0
29	7838.5
30	11464.5
31	16865.0
32	25065.0
33	32428.5
34	39203.5
35	48082.5
36	59922.0
37	71734.0
38	81317.5
39	90871.0
40	97806.5
41	102883.5
42	108700.0
43	116697.0
44	125982.0
45	135861.0
46	143221.0
47	149132.5
48	157349.0
49	164336.0
50	168300.5
51	169385.5
52	167917.5
53	165529.5
54	163151.0
55	156181.0
56	144610.0
57	130710.0
58	115799.5
59	99508.0
60	81702.5
61	67013.0
62	54926.5
63	42756.0
64	31421.5
65	22911.0
66	17026.0
67	12750.0
68	9601.0
69	7250.5
70	5458.0
71	4106.0
72	2924.0
73	2075.5
74	1572.5
75	1187.5
76	904.0
77	629.5
78	407.0
79	296.0
80	220.5
81	163.0
82	127.5
83	109.0
84	86.0
85	64.5
86	65.0
87	62.0
88	48.5
89	42.0
90	37.0
91	35.0
92	30.5
93	24.0
94	23.0
95	28.0
96	22.5
97	11.5
98	8.0
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05022049083176463
2	0.05798233458830091
3	0.04948515826535592
4	0.05626655860001394
5	0.048150665830021616
6	0.017076056264379837
7	0.014597713170187546
8	0.020834422714913196
9	0.020562077319947015
10-11	0.021814866136791466
12-13	0.027901785714285712
14-15	0.043874843129052495
16-17	0.02980820347904901
18-19	0.022999568604894372
20-21	0.026444737851216624
22-23	0.04752427142159939
24-25	0.04233609164749355
26-27	0.03062523966394757
28-29	0.020521225510702085
30-31	0.012650443596179322
32-33	0.009518471554068188
34-35	0.009341447047340166
36-37	0.0015523687513072579
38-39	0.006863103953147877
40-41	0.003554107404308722
42-43	0.20985574409119434
44-45	0.18420080788537962
46-47	0.19117284999651396
48-49	0.18942983946873038
50-51	0.18740086627623231
52-53	0.19194903437216762
54-55	0.19035581381161543
56-57	0.20099090148504498
58-59	0.19788616398243045
60-61	0.20259773931534547
62-63	0.19935682911524788
64-65	0.20097728421529668
66-67	0.19090050460154778
68-69	0.18955239489646517
70-71	0.17337507843547376
72-73	0.1806330832113226
74-75	0.18025179965836996
76-77	0.17850878913058635
78-79	0.20483097155406818
80-81	0.21354602419298613
82-83	0.21146258192149484
84-85	0.2068871792860629
86-87	0.22401770462943596
88-89	0.22211128686467266
90-91	0.22177085512096492
92-93	0.22551560430174997
94-95	0.2369949626995747
96-97	0.23613707470543122
98-99	0.22637349229589349
100-101	0.23591919838945827
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3671808.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.1493892843491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.04512602039685	37.87362975425966
2	13.335649253862252	13.6422063009068
3	4.883859640029361	7.4941931381394875
4	2.336446123640816	4.780311692800502
5	1.3350203144635555	3.4142736883505282
6	0.8336908675177291	2.558566723528263
7	0.6063092525220205	2.1708643588766083
8	0.40591608174008215	1.660988774536091
9	0.3167670000507056	1.458219473822608
>10	1.7485503474862372	16.194496006696344
>50	0.10726461310310044	3.7682404858952863
>100	0.04277494126950801	3.7578965499730996
>500	0.001821805991635564	0.6528928018559941
>1k	8.037379261235475E-4	0.5732202503587086
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.361726974830928E-5	0.0
12-13	0.0	0.0	0.0	5.446907899323712E-5	0.0
14-15	0.0	0.0	0.0	5.446907899323712E-5	4.085180924492784E-5
16-17	0.0	0.0	0.0	8.170361848985568E-5	1.361726974830928E-4
18-19	0.0	0.0	0.0	1.361726974830928E-4	1.361726974830928E-4
20-21	0.0	0.0	0.0	1.4978996723140207E-4	1.361726974830928E-4
22-23	0.0	0.0	0.0	2.3149358572125775E-4	1.6340723697971137E-4
24-25	0.0	0.0	0.0	5.174562504357526E-4	1.9064177647632992E-4
26-27	0.0	0.0	0.0	0.0010076779613748868	1.9064177647632992E-4
28-29	0.0	0.0	0.0	0.0027506884891584744	1.9064177647632992E-4
30-31	0.0	1.361726974830928E-5	0.0	0.010839346719654187	2.042590462246392E-4
32-33	0.0	2.723453949661856E-5	0.0	0.02820136564874852	2.1787631597294847E-4
34-35	2.723453949661856E-5	2.723453949661856E-5	0.0	0.05167753869483371	2.1787631597294847E-4
36-37	2.723453949661856E-5	2.723453949661856E-5	0.0	0.0892203513909224	2.1787631597294847E-4
38-39	2.723453949661856E-5	2.723453949661856E-5	0.0	0.1521457548978596	2.3149358572125775E-4
40-41	2.723453949661856E-5	2.723453949661856E-5	0.0	0.22631902321690023	2.4511085546956703E-4
42-43	2.723453949661856E-5	2.723453949661856E-5	0.0	0.3035017081503172	2.4511085546956703E-4
44-45	2.723453949661856E-5	2.723453949661856E-5	0.0	0.39879536184898556	2.587281252178763E-4
46-47	2.723453949661856E-5	2.723453949661856E-5	0.0	0.5048875104580631	2.859626647144949E-4
48-49	2.723453949661856E-5	2.723453949661856E-5	0.0	0.617856380290037	2.9957993446280413E-4
50-51	2.723453949661856E-5	2.723453949661856E-5	0.0	0.7468527766157708	2.9957993446280413E-4
52-53	2.723453949661856E-5	2.723453949661856E-5	0.0	0.8813641671895698	3.5404901345604124E-4
54-55	2.723453949661856E-5	2.723453949661856E-5	0.0	1.0393381135397057	4.085180924492784E-4
56-57	2.723453949661856E-5	2.723453949661856E-5	0.0	1.2135983145088196	4.085180924492784E-4
58-59	2.723453949661856E-5	2.723453949661856E-5	0.0	1.4024289941086243	4.085180924492784E-4
60-61	2.723453949661856E-5	2.723453949661856E-5	0.0	1.6095204324409118	4.085180924492784E-4
62-63	2.723453949661856E-5	2.723453949661856E-5	0.0	1.8323806691417417	4.3575263194589695E-4
64-65	2.723453949661856E-5	2.723453949661856E-5	0.0	2.074999564247368	4.3575263194589695E-4
66-67	2.723453949661856E-5	2.723453949661856E-5	0.0	2.354616581259151	4.3575263194589695E-4
68-69	2.723453949661856E-5	2.723453949661856E-5	0.0	2.65995662082549	4.3575263194589695E-4
70-71	2.723453949661856E-5	2.723453949661856E-5	0.0	2.984769356131911	4.3575263194589695E-4
72-73	2.723453949661856E-5	2.723453949661856E-5	0.0	3.330307575995259	5.174562504357527E-4
74-75	2.723453949661856E-5	2.723453949661856E-5	0.0	3.691832470543122	5.174562504357527E-4
76-77	2.723453949661856E-5	2.723453949661856E-5	0.0	4.091322313149272	5.310735201840619E-4
78-79	2.723453949661856E-5	2.723453949661856E-5	0.0	4.545934863696576	5.446907899323712E-4
80-81	2.723453949661856E-5	2.723453949661856E-5	0.0	5.02255292215715	5.583080596806805E-4
82-83	2.723453949661856E-5	2.723453949661856E-5	0.0	5.508566896744056	5.719253294289898E-4
84-85	2.723453949661856E-5	2.723453949661856E-5	0.0	6.021175399149411	5.719253294289898E-4
86-87	2.723453949661856E-5	2.723453949661856E-5	0.0	6.568807519347416	5.991598689256083E-4
88-89	2.723453949661856E-5	2.723453949661856E-5	0.0	7.163296664749355	5.991598689256083E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3560	0.0	48.25116	1
GTATCAA	6160	0.0	43.214687	1
TCAACGC	7430	0.0	35.193245	4
CAACGCA	7590	0.0	34.19846	5
ATCAACG	7805	0.0	33.25732	3
TATCAAC	7965	0.0	32.827568	2
AACGCAG	8110	0.0	32.172916	6
GTACATG	15250	0.0	31.986923	1
TACATGG	15830	0.0	30.1871	2
ACATGGG	16445	0.0	28.538773	3
CATGGGG	13405	0.0	26.728628	4
ACGCAGA	9675	0.0	26.678513	7
ATGGGGG	9280	0.0	26.385342	5
CGCAGAG	10215	0.0	24.807425	8
GAGTACA	10985	0.0	21.468689	1
GCAGAGT	11760	0.0	20.740952	9
TGGGGGG	9790	0.0	19.964714	6
AGTACAT	11165	0.0	19.933655	2
GTATAGG	1350	0.0	19.33206	1
GTATAGT	990	0.0	18.692982	1
>>END_MODULE