FastQCFastQC Report
Mon 15 Aug 2016
SRR2921371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3671808
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33950.049.2741971
GTATCAA59700.042.748081
TCAACGC78400.031.9578424
TATCAAC80850.031.282882
ATCAACG82050.030.6518613
CAACGCA82100.030.516335
AACGCAG86450.029.1441926
GTACATG149300.029.0303131
TACATGG154600.027.3481392
ACATGGG158850.026.108613
CATGGGG130100.023.8539334
ACGCAGA107550.023.072227
ATGGGGG94250.022.2949125
CCCTATA7900.022.2246112
CGCAGAG111600.022.0219448
GAGTACA110650.020.27251
TGGGGGG84050.019.7391666
GTATAAA12050.019.719671
GTAAGTA31550.019.2517472
GCAGAGT123850.018.5771319
Produced by FastQC (version 0.11.5)