##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921371_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3671808
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.444768081555463	33.0	31.0	34.0	30.0	34.0
2	31.568445027626716	34.0	31.0	34.0	29.0	34.0
3	31.648207912832042	34.0	31.0	34.0	28.0	34.0
4	35.145188691783446	37.0	35.0	37.0	33.0	37.0
5	34.89347591159451	37.0	35.0	37.0	32.0	37.0
6	34.848127135187895	37.0	35.0	37.0	32.0	37.0
7	34.71693046041623	37.0	35.0	37.0	32.0	37.0
8	34.68114590959004	37.0	35.0	37.0	32.0	37.0
9	36.303484005699644	39.0	37.0	39.0	32.0	39.0
10-11	36.15387174383846	39.0	37.0	39.0	32.0	39.0
12-13	36.069590376185246	39.0	37.0	39.0	32.0	39.0
14-15	37.30106081254793	40.0	38.0	41.0	32.0	41.0
16-17	37.276801782664016	40.0	38.0	41.0	31.5	41.0
18-19	37.24006252505578	40.0	38.0	41.0	31.0	41.0
20-21	37.19926559340793	40.0	38.0	41.0	31.0	41.0
22-23	37.096693100510706	40.0	37.5	41.0	31.0	41.0
24-25	37.02616803492993	40.0	37.0	41.0	31.0	41.0
26-27	36.88750936868159	40.0	37.0	41.0	30.0	41.0
28-29	36.74248313092623	40.0	37.0	41.0	30.0	41.0
30-31	36.60258883362093	40.0	37.0	41.0	30.0	41.0
32-33	36.561010270689536	40.0	36.5	41.0	30.0	41.0
34-35	36.70229516358154	40.0	37.0	41.0	30.0	41.0
36-37	36.6918494921303	40.0	37.0	41.0	30.0	41.0
38-39	36.6150775040525	40.0	37.0	41.0	30.0	41.0
40-41	36.501277436075085	40.0	37.0	41.0	30.0	41.0
42-43	36.40549859905529	40.0	36.5	41.0	30.0	41.0
44-45	36.28617604733145	40.0	36.0	41.0	29.0	41.0
46-47	36.14139846092171	40.0	36.0	41.0	29.0	41.0
48-49	35.996311353970576	40.0	35.5	41.0	28.0	41.0
50-51	35.84334529474308	40.0	35.0	41.0	28.0	41.0
52-53	35.67080495494318	39.0	35.0	41.0	28.0	41.0
54-55	35.46353254309594	39.0	35.0	41.0	27.0	41.0
56-57	35.21040955845186	39.0	35.0	41.0	27.0	41.0
58-59	34.982381431708845	38.5	35.0	40.5	26.0	41.0
60-61	34.697418955457366	38.0	34.5	40.0	26.0	41.0
62-63	34.3854362755351	37.5	34.0	40.0	26.0	41.0
64-65	34.073081707976016	37.0	34.0	40.0	26.0	41.0
66-67	33.74155143733006	36.5	34.0	39.0	25.5	41.0
68-69	33.37158356319284	36.0	34.0	39.0	24.5	41.0
70-71	32.99141172414244	35.0	33.5	38.5	24.0	40.0
72-73	32.59370846732727	35.0	33.0	37.5	22.5	39.5
74-75	32.188389207714565	35.0	33.0	37.0	22.0	39.0
76-77	29.44779724321097	33.0	29.0	34.5	16.5	36.5
78-79	31.117918611212787	34.5	31.5	36.0	19.5	37.0
80-81	31.32138744727393	35.0	32.0	35.5	20.0	37.0
82-83	31.1673842967824	35.0	32.0	35.0	20.0	37.0
84-85	30.95063263111797	35.0	32.0	35.0	19.0	36.0
86-87	30.720657507146342	35.0	32.0	35.0	18.0	36.0
88-89	30.45911087943596	35.0	32.0	35.0	12.0	36.0
90-91	30.28518389305759	35.0	32.0	35.0	8.5	35.0
92-93	30.14418237554905	34.0	32.0	35.0	6.0	35.0
94-95	30.051991416762533	34.0	32.0	35.0	2.0	35.0
96-97	29.869570386033256	34.0	31.5	35.0	2.0	35.0
98-99	29.62337968107265	34.0	31.0	35.0	2.0	35.0
100-101	28.54681658191278	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64888.0
3	22730.0
4	12770.0
5	8784.0
6	7750.0
7	7413.0
8	7794.0
9	8449.0
10	9415.0
11	10138.0
12	10743.0
13	11330.0
14	12461.0
15	13310.0
16	14092.0
17	15133.0
18	14428.0
19	12979.0
20	12863.0
21	12641.0
22	13424.0
23	15269.0
24	18299.0
25	21937.0
26	26394.0
27	32368.0
28	38499.0
29	46459.0
30	56993.0
31	70818.0
32	90472.0
33	120396.0
34	182218.0
35	264255.0
36	451918.0
37	894997.0
38	926250.0
39	110729.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.95823422123049	18.807065318933	13.038529548703421	26.196170911133095
2	15.286591591628396	20.424136110069384	38.078835054154744	26.210437244147478
3	18.42765962894105	24.66225399327695	28.34530739911743	28.564778978664574
4	13.330302899901461	15.85944381287718	34.699624854492896	36.11062843272846
5	14.147318181446703	38.14338991454249	33.5287114903169	14.180580413693905
6	34.54863309756142	34.34731324328954	16.812195974202083	14.291857684946956
7	29.784130458050424	31.829257631336777	20.098148020549043	18.28846389006376
8	27.681024034860762	35.15310138217471	19.104786545924966	18.061088037039557
9	27.99095159659764	15.266893040158964	18.4616679303493	38.28048743289409
10-11	26.530309306680156	25.241896097371008	26.860461613473042	21.367332982475798
12-13	26.736573342774662	22.25638651342666	27.170066452421153	23.836973691377526
14-15	23.924304668778454	23.919660933810164	24.23893473536907	27.917099662042315
16-17	24.27081873731636	27.064743199294405	25.0318474026341	23.632590660755135
18-19	24.325702614532613	26.730338374588946	26.224156603151556	22.71980240772688
20-21	24.54211706007152	25.715536563910245	26.43296593759447	23.309380438423766
22-23	24.53622895140559	25.268839460526323	26.015203438312696	24.17972814975539
24-25	24.29951327030527	25.541209839829804	25.928341263928473	24.230935625936457
26-27	23.916374171452695	25.960883375264267	26.09564338166455	24.027099071618487
28-29	23.997482821043477	26.53709087160385	25.62136557094573	23.84406073640694
30-31	24.87531915015321	25.81391715980844	25.59512387186626	23.71563981817209
32-33	24.42572184325767	25.80282360747438	25.604703669937933	24.166750879330017
34-35	24.549957931338607	26.090700524244433	25.86360601833807	23.495735526078892
36-37	24.407709012017467	26.097706548634918	25.98894460058274	23.50563983876487
38-39	24.547589118744334	26.13213871505274	25.705168349968904	23.615103816234026
40-41	24.637065590270492	25.817629723219998	25.66531336384381	23.879991322665695
42-43	24.24187826663905	25.86465200859106	25.507426613952394	24.3860431108175
44-45	23.94832751329222	25.905928597750954	25.893712912047878	24.25203097690895
46-47	24.18402089520977	25.613437257377065	25.704272969076232	24.498268878336933
48-49	23.923632447343994	25.97810739079844	25.887487416162223	24.210772745695348
50-51	24.211125797439966	25.745718547780218	25.968537362392834	24.074618292386983
52-53	24.416125472816933	25.67153724109541	25.85658557031557	24.055751715772082
54-55	23.530890453891015	25.485504484413323	26.401301714280578	24.582303347415085
56-57	23.872897296042957	25.621584918980428	25.8500831521671	24.65543463280951
58-59	24.06153444721899	25.69213383623789	26.080275159391107	24.166056557152007
60-61	24.48643664348513	25.74830891275936	26.039727051008864	23.725527392746645
62-63	24.22922266616256	25.637863086476116	26.16449442193266	23.968419825428665
64-65	23.773502661012724	25.301538306459854	26.305830526113617	24.61912850641381
66-67	23.939927800351803	25.734184821128036	25.867394956540856	24.458492421979305
68-69	23.74474968546126	25.68062205730741	26.064293257300115	24.510334999931217
70-71	23.77390840467507	25.4206397080179	26.056653267443373	24.748798619863656
72-73	23.688946229013563	26.012065761834414	25.69478515114756	24.604202858004463
74-75	23.585060497686484	25.873107476575047	25.87528676351509	24.666545262223384
76-77	23.473814999449253	25.28099449810814	26.054950126180852	25.19024037626176
78-79	23.363417524920497	25.393657601620333	26.28006341144758	24.96286146201159
80-81	23.784390248554637	25.735619397438768	25.66504560388619	24.814944750120404
82-83	23.750519263483486	25.76037199014455	25.87803539299903	24.61107335337293
84-85	23.643198663348286	25.818377026829882	25.66010868059448	24.87831562922735
86-87	23.240331088138262	26.139192100460306	25.732132382923144	24.88834442847829
88-89	23.15506419583929	25.654593864144175	25.975948506802677	25.214393433213857
90-91	23.511420684119557	25.314764963245608	26.129463606980885	25.044350745653954
92-93	23.67387026707268	25.241012007241974	25.949255768436647	25.1358619572487
94-95	23.474267016430485	25.341221948183367	25.931111344432246	25.2533996909539
96-97	23.01979506976069	25.16184025116	26.166499316549658	25.65186536252965
98-99	22.64142104916272	25.10764574866699	26.175864692902547	26.075068509267744
100-101	22.799612284238467	25.341906692209353	26.14116873588212	25.71731228767006
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	74.0
1	77.5
2	109.5
3	152.0
4	135.0
5	123.0
6	212.5
7	457.5
8	678.0
9	741.5
10	598.0
11	457.0
12	467.5
13	489.5
14	517.0
15	574.0
16	664.5
17	718.0
18	777.5
19	849.5
20	933.5
21	1062.0
22	1232.0
23	1461.5
24	1703.5
25	2148.5
26	2865.5
27	3879.0
28	5489.5
29	8127.0
30	12210.5
31	17777.5
32	25778.0
33	32911.5
34	39717.5
35	48487.0
36	60256.5
37	72230.5
38	81672.0
39	91298.0
40	98118.0
41	103246.5
42	109015.5
43	116820.0
44	126195.0
45	136248.5
46	143962.5
47	150206.5
48	158806.5
49	165925.5
50	169994.5
51	170896.5
52	169400.5
53	167689.5
54	164739.5
55	156833.0
56	144509.5
57	130297.0
58	115341.0
59	98456.0
60	80440.5
61	65899.5
62	54312.5
63	42071.5
64	30229.5
65	21734.5
66	15938.0
67	11877.0
68	8962.5
69	6651.0
70	4939.5
71	3591.0
72	2574.5
73	1848.0
74	1340.0
75	977.0
76	667.0
77	391.5
78	211.0
79	121.5
80	81.0
81	53.0
82	33.5
83	17.5
84	15.5
85	11.5
86	7.0
87	6.0
88	4.5
89	5.0
90	4.0
91	2.5
92	2.5
93	2.0
94	1.0
95	1.5
96	1.5
97	1.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14992613992888518
2	4.0851809244927834E-4
3	0.007135449348114063
4	0.004520933556438681
5	0.026635379627692954
6	0.0010621470403681238
7	1.9064177647632992E-4
8	0.0015796032908038764
9	0.0
10-11	0.09947415551139929
12-13	2.1787631597294847E-4
14-15	0.00529711793209231
16-17	4.085180924492784E-5
18-19	1.2255542773478351E-4
20-21	4.902217109391341E-4
22-23	1.9064177647632992E-4
24-25	0.0015659860210555673
26-27	0.0026008985219270727
28-29	0.006468203130446908
30-31	0.01455686136094262
32-33	0.014897293104650352
34-35	0.011683617444049362
36-37	7.489498361570103E-4
38-39	2.0425904622463917E-4
40-41	0.00472519260266332
42-43	0.008619731750679774
44-45	0.008143127309488948
46-47	0.009382298856585094
48-49	0.011765321062539217
50-51	0.00585542599177299
52-53	0.0017566277975318973
54-55	9.395916126333404E-4
56-57	0.006155005926235794
58-59	0.009150805270863836
60-61	0.007612053789304888
62-63	0.017539043435822355
64-65	0.03303549640939831
66-67	0.03517340775988287
68-69	0.02604983702851565
70-71	0.03938114411211044
72-73	0.025191949034372167
74-75	0.02403448110576588
76-77	0.23458470595412395
78-79	0.06642504183225267
80-81	0.051486896918357394
82-83	0.02023526284598759
84-85	0.03114269591438332
86-87	0.07996060796207209
88-89	0.2587008906783797
90-91	0.31312911786237185
92-93	0.04941707191661438
94-95	0.00510647615561598
96-97	0.009899755107020847
98-99	0.0011438506588579795
100-101	0.0012527888168444537
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3671808.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.05061336861091
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98433004797732	36.529104851984044
2	13.906405467187533	13.920482467706801
3	5.03059733299578	7.5535344638081074
4	2.4664151957254394	4.9378237347088305
5	1.384061065191924	3.4636552626234387
6	0.8571041409760118	2.5739152785975445
7	0.6069490396732493	2.126472020339484
8	0.44001036074096606	1.7618230754903283
9	0.31961449895587507	1.439721154281852
>10	1.842348094266641	16.771139920334697
>50	0.11583070944105368	3.9481654794932894
>100	0.043657404562452214	3.7574085006703317
>500	0.0017480113083578274	0.602772131089471
>1k	9.286309973853521E-4	0.6139816588717136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.723453949661856E-5
2	0.0	0.0	0.0	0.0	2.723453949661856E-5
3	0.0	0.0	0.0	0.0	2.723453949661856E-5
4	0.0	0.0	0.0	2.723453949661856E-5	2.723453949661856E-5
5	0.0	0.0	0.0	2.723453949661856E-5	2.723453949661856E-5
6	0.0	0.0	0.0	2.723453949661856E-5	2.723453949661856E-5
7	0.0	0.0	0.0	2.723453949661856E-5	2.723453949661856E-5
8	0.0	0.0	0.0	2.723453949661856E-5	2.723453949661856E-5
9	0.0	0.0	0.0	2.723453949661856E-5	2.723453949661856E-5
10-11	0.0	0.0	0.0	6.808634874154641E-5	5.446907899323712E-5
12-13	0.0	0.0	0.0	8.170361848985568E-5	5.446907899323712E-5
14-15	0.0	0.0	0.0	8.170361848985568E-5	5.446907899323712E-5
16-17	0.0	0.0	0.0	1.0893815798647424E-4	5.446907899323712E-5
18-19	0.0	0.0	0.0	1.9064177647632992E-4	5.446907899323712E-5
20-21	0.0	0.0	0.0	2.042590462246392E-4	5.446907899323712E-5
22-23	0.0	0.0	0.0	2.859626647144949E-4	9.532088823816496E-5
24-25	0.0	0.0	0.0	5.446907899323712E-4	1.361726974830928E-4
26-27	0.0	0.0	0.0	9.532088823816496E-4	1.361726974830928E-4
28-29	0.0	0.0	0.0	0.0025600467126821445	1.361726974830928E-4
30-31	0.0	0.0	0.0	0.010662322212926166	1.361726974830928E-4
32-33	0.0	0.0	0.0	0.028296686536986686	1.361726974830928E-4
34-35	0.0	0.0	0.0	0.05160945234609217	1.6340723697971137E-4
36-37	0.0	0.0	0.0	0.08919311685142578	1.6340723697971137E-4
38-39	1.361726974830928E-5	0.0	0.0	0.15205043400962143	1.6340723697971137E-4
40-41	2.723453949661856E-5	0.0	0.0	0.22561092518998815	1.6340723697971137E-4
42-43	2.723453949661856E-5	0.0	0.0	0.30310680732761625	1.6340723697971137E-4
44-45	2.723453949661856E-5	0.0	0.0	0.39834599194729137	1.6340723697971137E-4
46-47	2.723453949661856E-5	0.0	0.0	0.5046015477933486	1.6340723697971137E-4
48-49	2.723453949661856E-5	0.0	0.0	0.6169304059471519	1.6340723697971137E-4
50-51	2.723453949661856E-5	0.0	0.0	0.7449191243115109	1.9064177647632992E-4
52-53	2.723453949661856E-5	0.0	0.0	0.8791854040298404	1.9064177647632992E-4
54-55	2.723453949661856E-5	0.0	0.0	1.0363967832740708	1.9064177647632992E-4
56-57	2.723453949661856E-5	0.0	0.0	1.2099216516767761	1.9064177647632992E-4
58-59	2.723453949661856E-5	0.0	0.0	1.3985753067698528	1.9064177647632992E-4
60-61	2.723453949661856E-5	0.0	0.0	1.6047135362197587	1.9064177647632992E-4
62-63	2.723453949661856E-5	0.0	0.0	1.8266886503869484	1.9064177647632992E-4
64-65	2.723453949661856E-5	0.0	0.0	2.069130520985847	1.9064177647632992E-4
66-67	2.723453949661856E-5	2.723453949661856E-5	0.0	2.3480939090497106	1.9064177647632992E-4
68-69	2.723453949661856E-5	2.723453949661856E-5	0.0	2.6527667023983827	1.9064177647632992E-4
70-71	2.723453949661856E-5	2.723453949661856E-5	0.0	2.978083276685491	2.4511085546956703E-4
72-73	2.723453949661856E-5	2.723453949661856E-5	0.0	3.3249968407934185	2.723453949661856E-4
74-75	2.723453949661856E-5	2.723453949661856E-5	0.0	3.6886324121522693	2.723453949661856E-4
76-77	2.723453949661856E-5	2.723453949661856E-5	0.0	4.091213374991285	2.723453949661856E-4
78-79	2.723453949661856E-5	2.723453949661856E-5	0.0	4.54824979955379	2.723453949661856E-4
80-81	2.723453949661856E-5	2.723453949661856E-5	0.0	5.028667076274141	2.723453949661856E-4
82-83	2.723453949661856E-5	2.723453949661856E-5	0.0	5.518534738199818	2.9957993446280413E-4
84-85	2.723453949661856E-5	2.723453949661856E-5	0.0	6.036372272188524	2.9957993446280413E-4
86-87	2.723453949661856E-5	2.723453949661856E-5	0.0	6.588007869692532	2.9957993446280413E-4
88-89	2.723453949661856E-5	2.723453949661856E-5	0.0	7.188338823816496	2.9957993446280413E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3395	0.0	49.274197	1
GTATCAA	5970	0.0	42.74808	1
TCAACGC	7840	0.0	31.957842	4
TATCAAC	8085	0.0	31.28288	2
ATCAACG	8205	0.0	30.651861	3
CAACGCA	8210	0.0	30.51633	5
AACGCAG	8645	0.0	29.144192	6
GTACATG	14930	0.0	29.030313	1
TACATGG	15460	0.0	27.348139	2
ACATGGG	15885	0.0	26.10861	3
CATGGGG	13010	0.0	23.853933	4
ACGCAGA	10755	0.0	23.07222	7
ATGGGGG	9425	0.0	22.294912	5
CCCTATA	790	0.0	22.224611	2
CGCAGAG	11160	0.0	22.021944	8
GAGTACA	11065	0.0	20.2725	1
TGGGGGG	8405	0.0	19.739166	6
GTATAAA	1205	0.0	19.71967	1
GTAAGTA	3155	0.0	19.251747	2
GCAGAGT	12385	0.0	18.577131	9
>>END_MODULE