FastQCFastQC Report
Mon 15 Aug 2016
SRR2921370_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921370_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3035979
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.050.6775361
GTATCAA45600.042.5616571
TCAACGC57400.034.065674
CAACGCA59050.033.1072465
ATCAACG60000.032.587883
AACGCAG63600.030.8062256
TATCAAC62600.030.5515822
GTACATG90350.027.5734561
TACATGG92400.026.193962
ACGCAGA75600.025.039897
ACATGGG98500.023.945853
CGCAGAG76900.023.9406978
CATGGGG85250.021.2110524
GCAGAGT85500.019.9790559
GAGTACA69450.018.926381
ATGGGGG51800.018.6872835
GTATAAC7450.018.4714831
AGAGTAC71250.018.4141810-11
GTGGTAT13400.017.3520721
TCTATAC8300.017.1535223
Produced by FastQC (version 0.11.5)