##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921370_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3035979
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.570574104761594	31.0	31.0	34.0	27.0	34.0
2	30.63314041368534	33.0	31.0	34.0	27.0	34.0
3	30.685861792851664	33.0	31.0	34.0	27.0	34.0
4	34.15653303267249	37.0	35.0	37.0	30.0	37.0
5	34.14959721394647	37.0	35.0	37.0	30.0	37.0
6	34.191614302997486	37.0	35.0	37.0	30.0	37.0
7	34.11320401096319	37.0	35.0	37.0	30.0	37.0
8	34.07900845163949	37.0	35.0	37.0	30.0	37.0
9	35.65500321313158	39.0	37.0	39.0	30.0	39.0
10-11	35.55103312638197	39.0	37.0	39.0	30.0	39.0
12-13	35.43985169199128	39.0	37.0	39.0	30.0	39.0
14-15	36.59328704184054	40.0	37.0	41.0	30.0	41.0
16-17	36.54255546563398	40.0	37.0	41.0	29.5	41.0
18-19	36.488999594529474	40.0	37.0	41.0	29.0	41.0
20-21	36.42728194101474	40.0	37.0	41.0	29.0	41.0
22-23	36.31052652208727	40.0	37.0	41.0	28.0	41.0
24-25	36.211005247401246	40.0	36.5	41.0	28.0	41.0
26-27	36.063004388370274	40.0	36.0	41.0	27.0	41.0
28-29	35.919730999456846	40.0	36.0	41.0	27.0	41.0
30-31	35.77688399689194	40.0	36.0	41.0	27.0	41.0
32-33	35.59693413557867	40.0	35.5	41.0	26.0	41.0
34-35	35.433879153973066	39.0	35.0	41.0	25.0	41.0
36-37	35.26005351156908	39.0	35.0	41.0	25.0	41.0
38-39	35.4943057906527	39.5	35.0	41.0	25.5	41.0
40-41	35.61479806019739	40.0	35.5	41.0	26.0	41.0
42-43	35.45361331550713	40.0	35.0	41.0	25.0	41.0
44-45	35.333374835596686	40.0	35.0	41.0	24.5	41.0
46-47	35.18123774900946	40.0	35.0	41.0	24.0	41.0
48-49	35.03305852906097	39.5	35.0	41.0	23.5	41.0
50-51	33.30017467182744	37.0	32.5	39.5	20.5	40.0
52-53	33.46037044393258	37.5	33.5	39.5	21.0	40.0
54-55	34.31993699561163	39.0	34.0	40.5	22.0	41.0
56-57	34.32172060478679	39.0	34.0	41.0	22.0	41.0
58-59	34.135138451221174	38.0	34.0	41.0	21.0	41.0
60-61	33.8842865843275	38.0	34.0	40.0	20.0	41.0
62-63	33.586533701320064	37.0	34.0	40.0	19.0	41.0
64-65	33.28866092288517	37.0	33.5	40.0	18.0	41.0
66-67	32.936557696874715	36.0	33.0	39.0	16.0	41.0
68-69	32.569121196161106	36.0	33.0	39.0	13.0	41.0
70-71	32.181813016493194	35.0	33.0	38.5	9.5	40.5
72-73	31.820469113916793	35.0	32.5	37.5	7.0	40.0
74-75	31.426934771287943	35.0	32.0	37.0	4.0	39.0
76-77	31.05067344009955	35.0	32.0	36.5	2.0	39.0
78-79	30.70702695901388	35.0	32.0	36.0	2.0	38.0
80-81	30.3628216795966	35.0	31.0	35.5	2.0	37.0
82-83	30.071916670042846	35.0	31.0	35.0	2.0	37.0
84-85	29.842519826388788	35.0	31.0	35.0	2.0	36.0
86-87	29.622756942653425	35.0	31.0	35.0	2.0	36.0
88-89	29.42488683222117	35.0	31.0	35.0	2.0	36.0
90-91	29.169089608327333	35.0	31.0	35.0	2.0	35.0
92-93	28.977722177920203	34.5	30.0	35.0	2.0	35.0
94-95	28.76634044570137	34.0	30.0	35.0	2.0	35.0
96-97	28.506069870707275	34.0	30.0	35.0	2.0	35.0
98-99	28.268698992977225	34.0	30.0	35.0	2.0	35.0
100-101	27.21679711882065	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	93792.0
3	16570.0
4	11664.0
5	8894.0
6	8299.0
7	8556.0
8	9036.0
9	9674.0
10	10280.0
11	11034.0
12	11188.0
13	11488.0
14	12165.0
15	13084.0
16	13022.0
17	13460.0
18	12600.0
19	11966.0
20	12200.0
21	12943.0
22	14075.0
23	16256.0
24	19556.0
25	22730.0
26	27062.0
27	32280.0
28	38163.0
29	45057.0
30	53721.0
31	65315.0
32	81731.0
33	106827.0
34	150491.0
35	220581.0
36	370144.0
37	665387.0
38	680012.0
39	114668.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56654271564775	17.72774187255234	13.048107582981494	24.657607828818414
2	16.228780770107925	19.333211612918536	38.720538276676216	25.71746934029732
3	19.363234477542836	23.321673194257478	29.001286844364333	28.31380548383536
4	13.989106767454498	15.544557816291467	35.532588245103334	34.9337471711507
5	14.942359422931863	36.66588566922419	33.64764361208351	14.744111295760437
6	34.171248604005314	34.19493521596326	16.927724536892143	14.706091643139283
7	29.591760092345154	31.433590023349833	20.456731656151337	18.517918228153675
8	27.047873882516654	34.050758518174916	19.8322984313064	19.069069168002024
9	27.053797717524837	15.603304881186563	19.27640905333715	38.06648834795145
10-11	26.088070218440972	24.900488513926668	27.305121555232255	21.70631971240011
12-13	26.44717164951392	22.381035846650228	27.216170776089317	23.955621727746536
14-15	23.85121601157562	24.010295076424683	24.388031652367676	27.75045725963203
16-17	24.070970267226606	26.917843288298055	25.3008630352456	23.710323409229737
18-19	24.086888910486927	26.373978463094534	26.525135449904543	23.013997176514
20-21	24.573073487057574	25.546203841090737	26.449123578099098	23.431599093752588
22-23	24.507315767889118	25.293603668783327	26.110703127999287	24.08837743532827
24-25	24.311981626042513	25.557084155756854	26.04134469952846	24.08958951867217
26-27	24.071295412176603	25.708081029734757	26.112326912976926	24.108296645111718
28-29	24.149702670202778	25.999604658441367	25.842258718104993	24.00843395325086
30-31	24.518399436017464	25.582603510725388	25.78772250873399	24.11127454452316
32-33	24.22764583842559	25.613591008724974	25.759935091416875	24.39882806143256
34-35	24.551300507105207	25.684562378815563	25.805590345697247	23.958546768381986
36-37	24.54530332600443	25.600714773915694	25.816332748668035	24.037649151411845
38-39	24.445678621327797	25.667596087505558	25.78369545828946	24.103029832877187
40-41	24.554202723254388	25.466582209349397	25.82343413860948	24.155780928786736
42-43	24.384774033028904	25.502417004835	25.672286458023034	24.44052250411306
44-45	24.28114963260977	25.568492117782665	25.784006158863974	24.366352090743586
46-47	24.516699307197918	25.523256399141914	25.568896491537522	24.391147802122642
48-49	24.22246664343455	25.59586745550258	25.682162883527905	24.49950301753497
50-51	24.294395339908093	25.64571837391119	25.803869649029572	24.256016637151145
52-53	24.579224082327087	25.480466588817162	25.73323822427225	24.2070711045835
54-55	24.07834150806974	25.32227315642726	25.97911209921489	24.620273236288106
56-57	24.14113413990969	25.482553039249144	25.784986167979017	24.59132665286215
58-59	24.244668945412734	25.428238475605347	25.859868007863952	24.467224571117967
60-61	24.265359296570725	25.350213761215514	26.02493911101672	24.35948783119704
62-63	24.319372907062302	25.33340550099068	26.022271093074327	24.32495049887268
64-65	24.105534362513723	25.15309238448206	26.010845388119247	24.730527864884966
66-67	24.090489530659234	25.349024776675293	25.86853996843143	24.691945724234042
68-69	24.14987313066139	25.289469663394478	25.89563417014186	24.665023035802268
70-71	24.087086564421174	25.089321139542577	25.947797223649705	24.87579507238654
72-73	24.086921529706203	25.295450079499993	25.837274235122976	24.78035415567083
74-75	23.977075361359045	25.432064301443457	25.91534474813635	24.675515589061153
76-77	24.050093059145556	25.03308338828671	25.95408928167513	24.962734270892607
78-79	23.99910422716324	25.03610816077439	26.05619670828237	24.908590903780002
80-81	24.137513875928487	25.242752416466335	25.829857268147798	24.78987643945738
82-83	24.04004415756441	25.240200415532648	25.89219949594097	24.82755593096197
84-85	24.048375981263252	25.302382888722835	25.886833579336766	24.76240755067715
86-87	23.882759251318223	25.4088524928935	25.869755462617906	24.83863279317037
88-89	23.878776495070582	25.06909568785139	25.96421598490879	25.087911832169237
90-91	23.905118042915213	24.974515749728816	26.046900923705042	25.073465283650933
92-93	24.002134563320702	24.92739810728343	26.142871149425705	24.927596179970163
94-95	23.92221327858861	24.838981579122873	26.138651486407692	25.10015365588082
96-97	23.823434491171973	24.76973502297491	26.200081053932227	25.206749431920887
98-99	23.73130209177561	24.727126331520832	26.127090479867448	25.414481096836106
100-101	23.943699637636122	24.55529907854816	26.22449062131101	25.27651066250471
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	59.5
2	66.0
3	99.5
4	107.5
5	114.5
6	206.0
7	387.0
8	567.0
9	656.5
10	590.0
11	486.0
12	509.0
13	597.5
14	665.0
15	719.5
16	813.0
17	916.0
18	1017.5
19	1124.5
20	1234.0
21	1333.0
22	1430.5
23	1560.5
24	1749.0
25	2071.5
26	2560.0
27	3307.5
28	4483.5
29	6554.5
30	9851.0
31	14636.0
32	21133.5
33	26835.5
34	32151.5
35	38925.0
36	47260.0
37	55859.5
38	64069.5
39	71995.0
40	78682.5
41	84372.0
42	91105.5
43	99221.5
44	106663.0
45	115118.0
46	120586.0
47	124968.5
48	131154.5
49	134161.5
50	134370.5
51	133608.5
52	132621.5
53	132036.5
54	130329.5
55	124754.5
56	115415.5
57	104182.0
58	94683.5
59	84388.5
60	71115.5
61	59078.0
62	49275.5
63	38829.5
64	28800.5
65	21746.5
66	16798.0
67	13306.0
68	10414.0
69	8243.0
70	6561.5
71	4915.5
72	3645.0
73	2867.5
74	2389.0
75	1905.5
76	1445.5
77	988.0
78	626.0
79	437.5
80	314.5
81	211.5
82	163.5
83	141.5
84	110.0
85	85.5
86	71.0
87	57.5
88	49.0
89	42.0
90	38.0
91	39.5
92	28.5
93	15.0
94	14.5
95	15.5
96	11.0
97	6.5
98	6.5
99	5.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04766172625041214
2	0.056093932138529284
3	0.046904145252651616
4	0.053294176277240396
5	0.04604774934213972
6	0.016765596863482918
7	0.013537643046938072
8	0.019334784595018608
9	0.01943359950776998
10-11	0.021442836067047895
12-13	0.026350643400366074
14-15	0.04362678397973108
16-17	0.028886892827651313
18-19	0.0238473322773313
20-21	0.027190570158752744
22-23	0.04622891001551724
24-25	0.04113994200882154
26-27	0.031126697516682428
28-29	0.020718193373537825
30-31	0.013965841002194019
32-33	0.010704948881398718
34-35	0.010441442447395059
36-37	0.0017786684295247102
38-39	0.007164081174474527
40-41	0.004150226335557657
42-43	0.20749484762575765
44-45	0.18280758859003965
46-47	0.18934584198375548
48-49	0.18745188948935418
50-51	0.18618376477571155
52-53	0.19016929959001694
54-55	0.18801184066161195
56-57	0.19937555562801984
58-59	0.1959829102902227
60-61	0.20024842069065696
62-63	0.19754747974211942
64-65	0.19853562886963314
66-67	0.18939524944013117
68-69	0.18761658101060646
70-71	0.17132858955875518
72-73	0.18035368492338055
74-75	0.17920084427461455
76-77	0.17719160771533662
78-79	0.2039869182230839
80-81	0.21251793902395238
82-83	0.20993228214029147
84-85	0.2045798076995921
86-87	0.22095014491206955
88-89	0.2195502669814251
90-91	0.21927029139529622
92-93	0.22358520925210615
94-95	0.23494892421851402
96-97	0.23489951676213833
98-99	0.22496861803062537
100-101	0.23443838050263194
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3035979.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.527062745439565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.09680380675978	47.08400403168713
2	11.568689591577902	13.773002224007428
3	3.6339828749420398	6.48960979837583
4	1.730989519777848	4.12162887022057
5	0.9821558216982664	2.9232425612015724
6	0.6272273520510862	2.2402201164720554
7	0.43162235478974587	1.7985247697132505
8	0.3163118723798402	1.5063293339425776
9	0.2288260427434575	1.2259207983762388
>10	1.2989624586433353	13.520061852517395
>50	0.06234259884946513	2.5002516832508817
>100	0.020583321385639303	2.08253228353183
>500	0.001168521201230878	0.49091834631560943
>1k	3.3386320024868173E-4	0.2437533303876014
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.587660850091519E-5
2	0.0	0.0	0.0	0.0	6.587660850091519E-5
3	0.0	0.0	0.0	0.0	9.881491275137278E-5
4	0.0	0.0	0.0	0.0	9.881491275137278E-5
5	0.0	0.0	0.0	0.0	1.3175321700183037E-4
6	0.0	0.0	0.0	0.0	1.3175321700183037E-4
7	0.0	0.0	0.0	0.0	1.3175321700183037E-4
8	0.0	0.0	0.0	0.0	1.3175321700183037E-4
9	0.0	0.0	0.0	0.0	1.3175321700183037E-4
10-11	0.0	0.0	0.0	0.0	1.4822236912705918E-4
12-13	0.0	0.0	0.0	0.0	2.4703728187843197E-4
14-15	0.0	0.0	0.0	0.0	3.4585219462980474E-4
16-17	0.0	0.0	0.0	3.2938304250457594E-5	4.6113625950640634E-4
18-19	0.0	0.0	0.0	3.2938304250457594E-5	4.6113625950640634E-4
20-21	0.0	0.0	0.0	3.2938304250457594E-5	5.599511722577792E-4
22-23	0.0	0.0	0.0	4.940745637568639E-5	6.917043892596095E-4
24-25	0.0	0.0	0.0	3.952596510054912E-4	7.575809977605247E-4
26-27	0.0	0.0	0.0	7.575809977605247E-4	7.575809977605247E-4
28-29	0.0	0.0	0.0	0.0024868419709095486	7.575809977605247E-4
30-31	0.0	0.0	0.0	0.010095590252765253	7.575809977605247E-4
32-33	0.0	0.0	0.0	0.028656324697898108	7.575809977605247E-4
34-35	0.0	0.0	0.0	0.055303412836518295	7.905193020109822E-4
36-37	0.0	0.0	0.0	0.0983702456439916	8.234576062614399E-4
38-39	0.0	0.0	0.0	0.16437860736190862	8.563959105118975E-4
40-41	0.0	0.0	0.0	0.24176715319835876	8.563959105118975E-4
42-43	0.0	0.0	0.0	0.32482108736588755	8.893342147623551E-4
44-45	0.0	0.0	0.0	0.43312223174139214	9.552108232632702E-4
46-47	0.0	0.0	0.0	0.5551257106850871	0.0010046182796389566
48-49	0.0	0.0	0.0	0.6856272721254001	0.0010210874317641856
50-51	0.0	0.0	0.0	0.8205260971831492	0.001054025736014643
52-53	0.0	0.0	0.0	0.9646476474310264	0.001054025736014643
54-55	0.0	0.0	0.0	1.1372278925512989	0.001054025736014643
56-57	0.0	0.0	0.0	1.3408524894276277	0.001070494888139872
58-59	0.0	0.0	0.0	1.5533374901473298	0.0010869640402651007
60-61	0.0	0.0	0.0	1.7790636891757157	0.0010869640402651007
62-63	0.0	0.0	0.0	2.0092530284300385	0.0011528406487660158
64-65	0.0	0.0	0.0	2.274521661711099	0.001218717257266931
66-67	0.0	0.0	0.0	2.5928044956832705	0.001301063017893075
68-69	0.0	0.0	0.0	2.931640831507728	0.0013504704742687615
70-71	0.0	0.0	0.0	3.290207211578209	0.0014163470827696766
72-73	0.0	0.0	0.0	3.660022022550222	0.0015151619955210494
74-75	0.0	0.0	0.0	4.054820537296207	0.0015316311476462783
76-77	0.0	0.0	0.0	4.508858592236639	0.0016139769082724222
78-79	0.0	0.0	0.0	5.016092008541561	0.0016139769082724222
80-81	0.0	3.2938304250457594E-5	0.0	5.540173367470592	0.001630446060397651
82-83	0.0	3.2938304250457594E-5	0.0	6.079603976180336	0.0017292609731490239
84-85	0.0	4.940745637568639E-5	0.0	6.649683676995131	0.0017621992773994813
86-87	0.0	6.587660850091519E-5	0.0	7.284734182943954	0.001795137581649939
88-89	0.0	6.587660850091519E-5	0.0	7.96906039205146	0.0018116067337751677
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2575	0.0	50.677536	1
GTATCAA	4560	0.0	42.561657	1
TCAACGC	5740	0.0	34.06567	4
CAACGCA	5905	0.0	33.107246	5
ATCAACG	6000	0.0	32.58788	3
AACGCAG	6360	0.0	30.806225	6
TATCAAC	6260	0.0	30.551582	2
GTACATG	9035	0.0	27.573456	1
TACATGG	9240	0.0	26.19396	2
ACGCAGA	7560	0.0	25.03989	7
ACATGGG	9850	0.0	23.94585	3
CGCAGAG	7690	0.0	23.940697	8
CATGGGG	8525	0.0	21.211052	4
GCAGAGT	8550	0.0	19.979055	9
GAGTACA	6945	0.0	18.92638	1
ATGGGGG	5180	0.0	18.687283	5
GTATAAC	745	0.0	18.471483	1
AGAGTAC	7125	0.0	18.41418	10-11
GTGGTAT	1340	0.0	17.352072	1
TCTATAC	830	0.0	17.153522	3
>>END_MODULE