FastQCFastQC Report
Mon 15 Aug 2016
SRR2921370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3035979
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25450.050.048861
GTATCAA47900.045.8409581
TCAACGC62350.034.706414
TATCAAC63150.034.04132
CAACGCA63450.033.8686075
ATCAACG65250.033.3093573
AACGCAG67700.031.9510126
GTACATG92100.026.0086691
ACGCAGA82700.025.3505737
TACATGG95350.025.1334442
CGCAGAG86750.023.7834558
ACATGGG100100.023.1348693
CATGGGG86900.020.423644
GCAGAGT96250.020.003649
TATACCG2552.6528141E-718.6444845
AGAGTAC78850.018.38877710-11
TAACCCG1600.001184343217.8287875
GTGGTAT14900.017.5437981
GTATAAA10100.017.4111561
CAGAGTA88300.016.77073310-11
Produced by FastQC (version 0.11.5)