##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921370_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3035979
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.311636542940516	33.0	31.0	34.0	28.0	34.0
2	31.457099011554426	34.0	31.0	34.0	28.0	34.0
3	31.528691074608883	34.0	31.0	34.0	28.0	34.0
4	35.01665525354424	37.0	35.0	37.0	32.0	37.0
5	34.74653612557926	37.0	35.0	37.0	32.0	37.0
6	34.69458418520023	37.0	35.0	37.0	32.0	37.0
7	34.5589989258819	37.0	35.0	37.0	31.0	37.0
8	34.517826045568825	37.0	35.0	37.0	31.0	37.0
9	36.124907319846415	39.0	37.0	39.0	32.0	39.0
10-11	35.97774671695687	39.0	37.0	39.0	31.0	39.0
12-13	35.904433462813806	39.0	37.0	39.0	30.5	39.0
14-15	37.12878893431081	40.0	38.0	41.0	31.0	41.0
16-17	37.09924591046249	40.0	38.0	41.0	31.0	41.0
18-19	37.05604370122455	40.0	38.0	41.0	31.0	41.0
20-21	37.01474516128076	40.0	38.0	41.0	31.0	41.0
22-23	36.899155099557674	40.0	37.0	41.0	30.0	41.0
24-25	36.8255674693402	40.0	37.0	41.0	30.0	41.0
26-27	36.683689511686346	40.0	37.0	41.0	30.0	41.0
28-29	36.52639165158916	40.0	37.0	41.0	30.0	41.0
30-31	36.388118791335515	40.0	36.5	41.0	29.5	41.0
32-33	36.33395306752781	40.0	36.5	41.0	29.5	41.0
34-35	36.46233224933374	40.0	37.0	41.0	30.0	41.0
36-37	36.429191703895185	40.0	37.0	41.0	30.0	41.0
38-39	36.33333333333333	40.0	37.0	41.0	29.0	41.0
40-41	36.207046721996434	40.0	36.0	41.0	28.5	41.0
42-43	36.09775989886623	40.0	36.0	41.0	28.0	41.0
44-45	35.9540370667913	40.0	36.0	41.0	28.0	41.0
46-47	35.79860993768402	40.0	35.5	41.0	27.0	41.0
48-49	35.63316379988136	40.0	35.0	41.0	27.0	41.0
50-51	35.47113781090054	39.0	35.0	41.0	26.5	41.0
52-53	35.28716815893654	39.0	35.0	41.0	26.0	41.0
54-55	35.07125148098851	39.0	35.0	41.0	26.0	41.0
56-57	34.79744326294747	39.0	35.0	41.0	25.0	41.0
58-59	34.56063908874205	38.0	34.5	40.0	24.5	41.0
60-61	34.27623840612863	38.0	34.0	40.0	23.5	41.0
62-63	33.97413634942798	37.0	34.0	40.0	23.0	41.0
64-65	33.65255968503076	37.0	34.0	40.0	23.0	41.0
66-67	33.31206408212968	36.0	34.0	39.0	22.0	41.0
68-69	32.949372179451835	35.5	33.0	39.0	22.0	40.5
70-71	32.56762513838205	35.0	33.0	38.5	20.5	40.0
72-73	32.17726028407969	35.0	33.0	37.0	20.0	39.5
74-75	31.778224421183413	35.0	32.5	37.0	18.0	39.0
76-77	29.047243903860995	32.0	28.5	34.5	11.0	36.5
78-79	30.701744807852755	34.5	31.5	35.5	12.0	37.0
80-81	30.907168824290288	35.0	32.0	35.0	13.0	37.0
82-83	30.76006685158231	35.0	32.0	35.0	11.0	37.0
84-85	30.537402267934	35.0	32.0	35.0	7.5	36.0
86-87	30.30231944950871	35.0	32.0	35.0	4.0	36.0
88-89	30.033864529365978	35.0	31.0	35.0	2.0	35.5
90-91	29.857167160905924	34.0	31.0	35.0	2.0	35.0
92-93	29.71966884487673	34.0	31.0	35.0	2.0	35.0
94-95	29.617448934923463	34.0	31.0	35.0	2.0	35.0
96-97	29.42484780033063	34.0	31.0	35.0	2.0	35.0
98-99	29.158588547549243	34.0	31.0	35.0	2.0	35.0
100-101	28.07300379877463	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61137.0
3	20658.0
4	11429.0
5	8220.0
6	7236.0
7	7342.0
8	7372.0
9	8021.0
10	8649.0
11	9444.0
12	9959.0
13	10533.0
14	11488.0
15	12307.0
16	13383.0
17	14043.0
18	13439.0
19	11940.0
20	11545.0
21	11196.0
22	11883.0
23	13552.0
24	15965.0
25	18911.0
26	22870.0
27	27760.0
28	32825.0
29	39932.0
30	48373.0
31	60323.0
32	76497.0
33	100955.0
34	146062.0
35	219460.0
36	385652.0
37	729237.0
38	730125.0
39	86253.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.63474858083259	19.58307535037577	13.942031270668187	25.840144798123454
2	15.28191337985117	20.943699011320277	37.24248740434682	26.531900204481733
3	18.04552663955689	24.358250941677742	28.865819774326745	28.730402644438623
4	13.490622252629159	16.793544623478642	34.600905115096005	35.1149280087962
5	14.706271901443996	36.98373873381154	33.71922925367391	14.59076011107055
6	34.11163051755896	34.86283014442954	16.641940694558265	14.383598643453238
7	29.35821276525663	31.569660487981622	20.537487956916642	18.534638789845108
8	27.065457809399113	34.58089379678352	19.543466982880012	18.810181410937357
9	26.86553497240923	15.55218267320031	19.129249576495752	38.453032777894705
10-11	26.024895343481425	25.237230783737775	27.252171682519418	21.485702190261378
12-13	26.40131832594608	22.553884316766478	27.259297394590163	23.78549996269728
14-15	23.739614236847856	24.203886486925388	24.37863296864956	27.677866307577194
16-17	23.945352032417894	27.243400189889417	25.22375412861261	23.587493649080074
18-19	24.020178048384825	26.598346987662108	26.55885387631146	22.822621087641604
20-21	24.518138493567356	25.65454985333307	26.438601556245544	23.388710096854027
22-23	24.402872162295385	25.408234368548705	26.15720409668012	24.031689372475785
24-25	24.152229314678554	25.73607520324065	26.055861575337797	24.055833906743
26-27	24.015495344923934	25.841967706557522	26.150856778076996	23.991680170441548
28-29	24.08901117959676	26.1286860082678	25.895136084006822	23.887166728128616
30-31	24.44764435764927	25.76530250874733	25.797784812315864	23.98926832128754
32-33	24.240027356247925	25.763118744037232	25.688468867113993	24.308385032600846
34-35	24.49459773789694	25.858698360947898	25.82593741357838	23.820766487576783
36-37	24.46003497081072	25.80470002821183	25.849232886923268	23.886032114054185
38-39	24.415549038873586	25.795009764592457	25.8113966249326	23.978044571601355
40-41	24.454635740320143	25.636509471097547	25.819409285480177	24.089445503102134
42-43	24.390945706322466	25.609531938106507	25.61229898169502	24.38722337387601
44-45	24.282680181274504	25.652318925199975	25.725826840518458	24.339174053007063
46-47	24.431785333750813	25.64756286955386	25.553629750000866	24.367022046694462
48-49	24.157847568495118	25.75896458014307	25.684333522916976	24.398854328444845
50-51	24.33389485806293	25.727473184833432	25.714066425059507	24.22456553204413
52-53	24.539743523865614	25.597002683868723	25.64425366182727	24.219000130438392
54-55	23.997465047230623	25.467447794004784	25.89551752101646	24.639569637748135
56-57	24.11730631862634	25.61156882822643	25.694232961989815	24.576891891157405
58-59	24.198606745237296	25.54776949943135	25.779857841566887	24.473765913764463
60-61	24.21622835977954	25.502729388272137	25.874498703534393	24.40654354841393
62-63	24.170821898244746	25.567147724186075	25.8773837846065	24.384646592962678
64-65	23.986189722127218	25.314103590593167	25.876511456725005	24.823195230554607
66-67	23.999099143923125	25.588645523018783	25.651728513478737	24.76052681957935
68-69	24.015369725923026	25.465428412577477	25.721376356485393	24.797825505014107
70-71	23.956078392089285	25.30023275236134	25.77629840543132	24.967390450118057
72-73	23.95327962034873	25.538015288251394	25.562066370113534	24.946638721286345
74-75	23.88076223792697	25.621263380091204	25.572799441495114	24.92517494048671
76-77	23.877437351655793	25.241733581480236	25.7735193972896	25.107309669574366
78-79	23.8116787767822	25.32987361423088	25.76985891399538	25.088588694991536
80-81	23.9319750354767	25.500167084873183	25.557905289570936	25.009952590079177
82-83	23.835994850748296	25.567319547636757	25.54788219315953	25.048803408455413
84-85	23.76712782372069	25.57312951002077	25.55625991953337	25.103482746725174
86-87	23.56585224748871	25.71214772119487	25.524793126210938	25.197206905105485
88-89	23.535572660456417	25.492584158049635	25.555164406072166	25.41667877542178
90-91	23.541296050075204	25.38519895007018	25.6397183798515	25.43378662000312
92-93	23.569051697220004	25.356411669669683	25.660602135973683	25.41393449713663
94-95	23.42268376068939	25.34370257691701	25.67244222927647	25.561171433117124
96-97	23.23927272415826	25.261221974813257	25.757353901566514	25.742151399461967
98-99	23.134298614990566	25.25438612671677	25.664325598431585	25.946989659861075
100-101	23.0660026219227	25.272337397972294	25.628668550761873	26.032991429343138
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	46.5
2	66.5
3	89.5
4	78.0
5	89.5
6	178.0
7	354.0
8	489.5
9	499.5
10	445.0
11	358.5
12	344.0
13	384.0
14	423.5
15	464.5
16	522.0
17	566.0
18	609.5
19	708.0
20	818.5
21	915.0
22	1049.5
23	1223.0
24	1454.5
25	1832.0
26	2415.0
27	3367.0
28	4854.0
29	7087.5
30	10441.0
31	15338.0
32	22074.0
33	27866.0
34	32957.0
35	39339.5
36	47661.0
37	56593.5
38	64648.0
39	72323.0
40	78771.5
41	84914.5
42	91890.0
43	99727.5
44	107303.5
45	115629.5
46	121861.0
47	126655.0
48	132708.0
49	135979.0
50	135524.5
51	134128.0
52	133390.0
53	132776.0
54	130872.5
55	124753.0
56	114906.0
57	103938.5
58	94067.5
59	83626.0
60	69984.0
61	57611.5
62	48181.0
63	37740.0
64	27582.5
65	20691.5
66	16006.5
67	12538.0
68	9892.5
69	7680.5
70	5944.5
71	4563.5
72	3401.0
73	2655.0
74	2151.0
75	1659.0
76	1214.0
77	793.5
78	470.5
79	269.5
80	171.5
81	117.5
82	77.0
83	45.0
84	25.5
85	23.5
86	19.0
87	14.5
88	14.0
89	8.5
90	3.0
91	3.0
92	2.5
93	2.0
94	1.5
95	1.5
96	1.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1465095773060354
2	3.6232134675503357E-4
3	0.005731264939579621
4	0.0040184731185558265
5	0.02605419866211196
6	0.001152840648766016
7	1.3175321700183037E-4
8	0.0017786684295247102
9	0.0
10-11	0.0975632571898554
12-13	2.4703728187843197E-4
14-15	0.005023091398194784
16-17	4.94074563756864E-5
18-19	2.1409897762797437E-4
20-21	6.093586286334655E-4
22-23	2.799755861288896E-4
24-25	0.0015151619955210494
26-27	0.0032773612729205303
28-29	0.007427587608478187
30-31	0.015810386040219646
32-33	0.015645694518967357
34-35	0.011610752248286303
36-37	6.093586286334655E-4
38-39	3.2938304250457596E-4
40-41	0.005401881897075046
42-43	0.008168699454113483
44-45	0.008283983518990085
46-47	0.010161466861266167
48-49	0.012500086463048657
50-51	0.0064888459373401465
52-53	0.0020751131677788285
54-55	8.234576062614398E-4
56-57	0.006604130002216748
58-59	0.009272132646503813
60-61	0.007427587608478187
62-63	0.01806665988137599
64-65	0.03270773612070439
66-67	0.035836875024497866
68-69	0.026070667814237187
70-71	0.039805940686678004
72-73	0.02544484003347849
74-75	0.02412730786346019
76-77	0.23478423269726176
78-79	0.0663377447604216
80-81	0.052470718670978946
82-83	0.019318315442893377
84-85	0.03069849956142648
86-87	0.07992479526373536
88-89	0.2591256395383499
90-91	0.31261744564109306
92-93	0.05044501295957581
94-95	0.004677239203564979
96-97	0.009700330601759762
98-99	0.0014163470827696766
100-101	0.0012845938657678462
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3035979.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.14291663141916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76484070874163	46.58382407524117
2	11.610011090818764	13.732998360682922
3	3.843531921568076	6.81953064022497
4	1.7336075920281655	4.101224371476684
5	0.9890861590560833	2.9248720123172265
6	0.6510380601090447	2.3102573827746054
7	0.45579405290102315	1.8869892770275307
8	0.3102792409700119	1.4680655424919546
9	0.2551944745011376	1.3583650977197643
>10	1.3030557659219975	13.601344909692495
>50	0.06175545702092109	2.4454103154472846
>100	0.020183705611412438	2.021352615628342
>500	0.0012303088464767316	0.4700227112001201
>1k	3.914619053218185E-4	0.27574268807512353
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.587660850091519E-5
2	0.0	0.0	0.0	0.0	6.587660850091519E-5
3	0.0	0.0	0.0	0.0	6.587660850091519E-5
4	0.0	0.0	0.0	0.0	6.587660850091519E-5
5	0.0	0.0	0.0	0.0	6.587660850091519E-5
6	0.0	0.0	0.0	0.0	9.881491275137278E-5
7	0.0	0.0	0.0	0.0	9.881491275137278E-5
8	0.0	0.0	0.0	0.0	9.881491275137278E-5
9	0.0	0.0	0.0	0.0	1.3175321700183037E-4
10-11	0.0	0.0	0.0	0.0	1.3175321700183037E-4
12-13	0.0	0.0	0.0	0.0	2.799755861288896E-4
14-15	0.0	0.0	0.0	0.0	3.7879049888026235E-4
16-17	0.0	0.0	0.0	0.0	4.2819795525594873E-4
18-19	0.0	0.0	0.0	0.0	4.6113625950640634E-4
20-21	0.0	0.0	0.0	0.0	4.776054116316351E-4
22-23	0.0	0.0	0.0	1.6469152125228797E-5	5.105437158820928E-4
24-25	0.0	0.0	0.0	2.9644473825411836E-4	5.270128680073215E-4
26-27	0.0	0.0	0.0	6.752352371343806E-4	5.434820201325503E-4
28-29	0.0	0.0	0.0	0.002437434514533862	5.928894765082367E-4
30-31	0.0	0.0	0.0	0.010079121100640024	5.928894765082367E-4
32-33	0.0	0.0	0.0	0.029216275870155888	6.258277807586943E-4
34-35	0.0	0.0	0.0	0.056044524682153596	6.258277807586943E-4
36-37	0.0	0.0	0.0	0.09871609783862141	6.258277807586943E-4
38-39	0.0	0.0	0.0	0.16492208938204117	6.917043892596095E-4
40-41	0.0	0.0	0.0	0.2412236711782262	7.246426935100671E-4
42-43	0.0	0.0	0.0	0.32435995110638116	7.740501498857535E-4
44-45	0.0	0.0	0.0	0.4323317124393812	8.234576062614399E-4
46-47	0.0	0.0	0.0	0.5541375615575734	8.563959105118975E-4
48-49	0.0	0.0	0.0	0.6829263311768625	9.552108232632702E-4
50-51	0.0	0.0	0.0	0.8163594016954663	0.001054025736014643
52-53	0.0	0.0	0.0	0.9597563092498334	0.0011034331923903294
54-55	0.0	0.0	0.0	1.131611911676596	0.0012022481051417021
56-57	3.2938304250457594E-5	0.0	0.0	1.3334743092755252	0.0012516555615173885
58-59	3.2938304250457594E-5	0.0	0.0	1.543851258523198	0.001301063017893075
60-61	3.2938304250457594E-5	0.0	0.0	1.7676176284486815	0.0013669396263939902
62-63	3.2938304250457594E-5	0.0	0.0	1.9959953609692294	0.0014822236912705917
64-65	3.2938304250457594E-5	0.0	0.0	2.2590571278655087	0.0015151619955210494
66-67	3.2938304250457594E-5	0.0	0.0	2.5741614154775116	0.0015481002997715068
68-69	3.2938304250457594E-5	0.0	0.0	2.910263872049181	0.0015810386040219645
70-71	3.2938304250457594E-5	0.0	0.0	3.2662775335402516	0.0015810386040219645
72-73	3.2938304250457594E-5	0.0	0.0	3.634017231344486	0.0016798535167733375
74-75	3.2938304250457594E-5	0.0	0.0	4.028552239656467	0.0017292609731490239
76-77	4.940745637568639E-5	0.0	0.0	4.482112689185268	0.0017786684295247102
78-79	6.587660850091519E-5	0.0	0.0	4.990186032248577	0.0017786684295247102
80-81	6.587660850091519E-5	0.0	0.0	5.515469639282749	0.0017786684295247102
82-83	6.587660850091519E-5	0.0	0.0	6.056020150337009	0.0018116067337751677
84-85	6.587660850091519E-5	0.0	0.0	6.628965483621593	0.0018116067337751677
86-87	6.587660850091519E-5	0.0	0.0	7.264477125829922	0.0018280758859003966
88-89	6.587660850091519E-5	0.0	0.0	7.949280940349061	0.001877483342276083
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2545	0.0	50.04886	1
GTATCAA	4790	0.0	45.840958	1
TCAACGC	6235	0.0	34.70641	4
TATCAAC	6315	0.0	34.0413	2
CAACGCA	6345	0.0	33.868607	5
ATCAACG	6525	0.0	33.309357	3
AACGCAG	6770	0.0	31.951012	6
GTACATG	9210	0.0	26.008669	1
ACGCAGA	8270	0.0	25.350573	7
TACATGG	9535	0.0	25.133444	2
CGCAGAG	8675	0.0	23.783455	8
ACATGGG	10010	0.0	23.134869	3
CATGGGG	8690	0.0	20.42364	4
GCAGAGT	9625	0.0	20.00364	9
TATACCG	255	2.6528141E-7	18.644484	5
AGAGTAC	7885	0.0	18.388777	10-11
TAACCCG	160	0.0011843432	17.828787	5
GTGGTAT	1490	0.0	17.543798	1
GTATAAA	1010	0.0	17.411156	1
CAGAGTA	8830	0.0	16.770733	10-11
>>END_MODULE