FastQCFastQC Report
Mon 15 Aug 2016
SRR2921369_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921369_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4522977
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33650.041.180851
GTATCAA53800.040.4881481
TCAACGC63900.033.272044
CAACGCA64600.032.8347785
ATCAACG68600.030.9232923
AACGCAG69850.030.4933076
TATCAAC71000.030.2797162
GTACATG130100.030.0571251
TACATGG137650.027.7199062
ACATGGG143250.026.0724833
CATGGGG121800.023.572764
ACGCAGA87200.023.5054577
CGCAGAG89650.022.8131948
GAGTACA97150.021.591211
AGTACAT97300.019.9490572
ATGGGGG80050.019.5026515
GTATAGC13650.017.7310731
GCAGAGT110400.017.1069729
GTGTAGC25100.017.0163461
AGAGTAC87500.016.75751110-11
Produced by FastQC (version 0.11.5)