##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921369_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4522977
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.482890803999226	31.0	31.0	34.0	26.0	34.0
2	30.531464564157634	33.0	31.0	34.0	26.0	34.0
3	30.587625362675954	33.0	31.0	34.0	26.0	34.0
4	34.046598291346605	37.0	35.0	37.0	30.0	37.0
5	34.038370303452794	37.0	35.0	37.0	30.0	37.0
6	34.08402430523083	37.0	35.0	37.0	30.0	37.0
7	33.99633957899852	37.0	35.0	37.0	30.0	37.0
8	33.962503899533424	37.0	35.0	37.0	30.0	37.0
9	35.531207874813425	39.0	37.0	39.0	30.0	39.0
10-11	35.42905634939112	39.0	37.0	39.0	30.0	39.0
12-13	35.31584208807606	39.0	35.0	39.0	29.0	39.0
14-15	36.465312226880656	40.0	37.0	41.0	29.0	41.0
16-17	36.41015154399415	40.0	37.0	41.0	29.0	41.0
18-19	36.34844616720359	40.0	37.0	41.0	28.0	41.0
20-21	36.28219909143911	40.0	37.0	41.0	27.5	41.0
22-23	36.167941490748234	40.0	36.5	41.0	27.0	41.0
24-25	36.07545926057108	40.0	36.0	41.0	27.0	41.0
26-27	35.92980364481181	40.0	36.0	41.0	27.0	41.0
28-29	35.79227862533902	40.0	36.0	41.0	27.0	41.0
30-31	35.657408715542886	40.0	36.0	41.0	26.0	41.0
32-33	35.49415086567984	40.0	35.5	41.0	25.0	41.0
34-35	35.33822889216549	39.0	35.0	41.0	25.0	41.0
36-37	35.17486911828205	39.0	35.0	41.0	25.0	41.0
38-39	35.42322943494959	39.5	35.0	41.0	25.5	41.0
40-41	35.55793120327607	40.0	35.5	41.0	25.5	41.0
42-43	35.40520292276525	40.0	35.0	41.0	24.5	41.0
44-45	35.29946957501663	40.0	35.0	41.0	24.0	41.0
46-47	35.162494967363315	40.0	35.0	41.0	24.0	41.0
48-49	35.02419766892469	39.5	35.0	41.0	23.0	41.0
50-51	33.29793960924409	37.0	32.5	39.5	20.0	40.0
52-53	33.453354063042994	37.5	33.5	39.5	21.0	40.0
54-55	34.3117425757416	39.0	34.0	40.5	21.5	41.0
56-57	34.316831701775186	39.0	34.0	41.0	22.0	41.0
58-59	34.12934589320264	38.0	34.0	41.0	21.0	41.0
60-61	33.871005202989096	38.0	34.0	40.0	20.0	41.0
62-63	33.572068794512994	37.0	34.0	40.0	18.5	41.0
64-65	33.26776866210021	37.0	33.5	40.0	18.0	41.0
66-67	32.92078801196645	36.0	33.0	39.0	16.0	41.0
68-69	32.548823595609704	36.0	33.0	39.0	13.0	41.0
70-71	32.16947510456056	35.0	33.0	38.5	9.5	40.5
72-73	31.81708021066656	35.0	32.5	37.0	7.5	39.5
74-75	31.42557998857832	35.0	32.0	37.0	4.5	39.0
76-77	31.063321016224492	35.0	32.0	36.5	2.0	39.0
78-79	30.73719444074113	35.0	32.0	36.0	2.0	38.0
80-81	30.40588621166988	35.0	31.5	35.5	2.0	37.0
82-83	30.122586959871782	35.0	31.0	35.0	2.0	37.0
84-85	29.905020189136494	35.0	31.0	35.0	2.0	36.0
86-87	29.697691586758012	35.0	31.0	35.0	2.0	36.0
88-89	29.514226802391434	35.0	31.0	35.0	2.0	36.0
90-91	29.27537239300576	35.0	31.0	35.0	2.0	35.0
92-93	29.095459804460646	34.5	30.5	35.0	2.0	35.0
94-95	28.899302715888233	34.0	30.0	35.0	2.0	35.0
96-97	28.65825351311758	34.0	30.0	35.0	2.0	35.0
98-99	28.440454152209927	34.0	30.0	35.0	2.0	35.0
100-101	27.40420756063982	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	151285.0
3	26047.0
4	18284.0
5	13872.0
6	12453.0
7	12760.0
8	13331.0
9	14153.0
10	14985.0
11	16436.0
12	16057.0
13	16205.0
14	17024.0
15	18056.0
16	17635.0
17	17873.0
18	17526.0
19	17126.0
20	17345.0
21	18522.0
22	20069.0
23	23348.0
24	27550.0
25	32952.0
26	38973.0
27	46802.0
28	55822.0
29	66118.0
30	78962.0
31	96684.0
32	121679.0
33	157819.0
34	222807.0
35	325030.0
36	550338.0
37	1006336.0
38	1017409.0
39	167289.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.397957675880804	16.968999710666328	12.095939330179272	25.537103283273595
2	16.498927865347053	18.653151996241057	38.72088462556917	26.127035512842724
3	19.914085907456542	22.45678027868992	28.39971730635873	29.22941650749481
4	13.659662511306808	14.535073167677872	35.1248211760318	36.68044314498351
5	14.799175378136017	36.748273558570716	33.79427807983436	14.6582729834589
6	35.59202697978263	33.524294917452806	16.366587862857045	14.517090239907516
7	30.582271696128927	30.873735914092244	19.85978937005828	18.684203019720545
8	27.847707418148932	34.448888068225166	18.922886235000007	18.780518278625898
9	27.30597424194176	15.252030039273961	18.45517991720624	38.98681580157803
10-11	26.65686198519479	24.590404289970156	26.937280592977935	21.81545313185712
12-13	26.848830990072198	21.813221802440687	26.95575751805726	24.382189689429854
14-15	23.91470577435984	23.48033150290667	23.91751489608683	28.687447826646668
16-17	24.51559068930948	26.59990034591424	24.78235224777907	24.102156716997214
18-19	24.540895318537633	26.114957454821525	25.812606606206817	23.531540620434026
20-21	24.899239885756735	25.27621745054934	25.9722304693897	23.852312194304222
22-23	24.772978254774653	25.019766627164746	25.66476309079377	24.542492027266828
24-25	24.578865523955887	25.22871965763135	25.611503328344494	24.58091149006827
26-27	24.386602811131244	25.4433839821451	25.65886268689759	24.511150519826064
28-29	24.46606379569879	25.778925787517064	25.425522481169406	24.329487935614743
30-31	24.8470401710616	25.381861964389067	25.32668044638321	24.44441741816612
32-33	24.49546914771685	25.408096219485998	25.387112220282905	24.709322412514247
34-35	24.831174551489426	25.503082202495904	25.428853289328142	24.236889956686532
36-37	24.84836632723158	25.374919272052242	25.453508108833546	24.32320629188263
38-39	24.71653611555421	25.576302861481683	25.42315055293146	24.284010470032648
40-41	24.85023042238835	25.330775467571833	25.471175809750683	24.347818300289138
42-43	24.55028547500515	25.307708157473197	25.392320423260728	24.74968594426092
44-45	24.516663063586684	25.320733185429855	25.607443845112453	24.55515990587101
46-47	24.68738675038167	25.34023618830914	25.382512353899685	24.589864707409504
48-49	24.518117258244583	25.459499211426344	25.41411192429693	24.608271606032144
50-51	24.46882538478671	25.4923168916302	25.610612273015683	24.428245450567402
52-53	24.781859152246426	25.308203797119955	25.51344963303072	24.3964874176029
54-55	24.258614899738348	25.16330009737754	25.82595913333103	24.752125869553083
56-57	24.379288114791322	25.385229722215453	25.62538630417397	24.610095858819257
58-59	24.49872011997975	25.33662893669717	25.641784233606074	24.522866709717007
60-61	24.584878206127975	25.30744224007146	25.777594588750723	24.330084965049835
62-63	24.58431652854581	25.293225060847057	25.775781314123687	24.34667709648345
64-65	24.36106915762382	25.168636403663992	25.718445854524692	24.751848584187496
66-67	24.35170172447956	25.318568356006637	25.635345213231552	24.694384706282253
68-69	24.40621056401939	25.19872356176418	25.763578049710294	24.631487824506134
70-71	24.304793303938844	25.10320849977365	25.734297469044094	24.85770072724341
72-73	24.330255839600284	25.36287706644405	25.574424766784777	24.73244232717089
74-75	24.222415781759345	25.436879441263876	25.730112911165588	24.61059186581119
76-77	24.350716408280682	25.073342962890692	25.687584393826825	24.888356235001805
78-79	24.23148312668072	25.104670365337128	25.8129815623301	24.850864945652056
80-81	24.439298912181894	25.235567629498632	25.607867235961795	24.717266222357672
82-83	24.428226108419132	25.32817758634824	25.686000163955857	24.557596141276772
84-85	24.38132810975974	25.35664947175124	25.57284967644189	24.689172742047134
86-87	24.17683226780284	25.482036955132493	25.655073021264226	24.68605775580044
88-89	24.108481758596877	25.2799298199198	25.696086704927573	24.915501716555745
90-91	24.260571104429197	25.19660935827751	25.757377005590502	24.785442531702788
92-93	24.40420871906758	25.111039167953003	25.81503325413677	24.66971885884265
94-95	24.335266969178175	25.045315781017123	25.789183828377354	24.830233421427344
96-97	24.22791924673308	24.89675578983691	25.872213979823982	25.00311098360603
98-99	24.082675640364265	24.93593698202247	25.817484882811875	25.163902494801388
100-101	24.305468447005374	24.922355809186104	25.886797052468282	24.88537869134024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	30.0
1	28.5
2	51.5
3	80.0
4	82.5
5	103.5
6	192.0
7	354.5
8	523.0
9	580.5
10	518.0
11	478.0
12	537.5
13	633.5
14	707.0
15	800.5
16	919.0
17	980.0
18	1069.0
19	1269.0
20	1402.5
21	1501.0
22	1638.5
23	1775.5
24	1953.5
25	2317.0
26	2931.5
27	3903.0
28	5438.5
29	7922.0
30	11871.0
31	17830.5
32	26443.0
33	33816.5
34	40580.5
35	50019.5
36	62643.0
37	75704.5
38	87631.0
39	99652.5
40	110054.0
41	119860.0
42	129945.5
43	141185.0
44	153156.5
45	164807.5
46	174490.0
47	183558.5
48	193829.0
49	200794.5
50	203900.0
51	206837.5
52	208524.0
53	208570.5
54	207219.0
55	200164.5
56	188107.5
57	172310.0
58	156093.5
59	137478.5
60	115085.5
61	95582.0
62	78267.0
63	60099.0
64	43248.0
65	31461.0
66	23445.0
67	17638.5
68	13162.5
69	9781.5
70	7288.0
71	5282.0
72	3784.0
73	2823.5
74	2156.0
75	1620.5
76	1201.5
77	829.5
78	535.5
79	358.5
80	270.0
81	217.5
82	168.0
83	123.0
84	101.5
85	93.5
86	89.0
87	76.5
88	70.5
89	67.0
90	57.5
91	41.5
92	33.0
93	28.0
94	23.5
95	21.5
96	19.0
97	16.5
98	10.5
99	8.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04963545027976043
2	0.05651145252341544
3	0.048242562365450894
4	0.054610049973723054
5	0.04680545578719503
6	0.01715684161117777
7	0.014105753798880693
8	0.01972152412006517
9	0.019832070779931005
10-11	0.02183296532350264
12-13	0.026100066394323915
14-15	0.044019679958575954
16-17	0.029538067516151418
18-19	0.023833859867074276
20-21	0.027028658337196938
22-23	0.046418542477664596
24-25	0.04168714543540681
26-27	0.03131786873999138
28-29	0.020959646710562534
30-31	0.01391782447710877
32-33	0.010689862009026356
34-35	0.010623534013106853
36-37	0.0015476532381217061
38-39	0.007848812850474366
40-41	0.004333429066740778
42-43	0.20886685915051081
44-45	0.1839717513487245
46-47	0.19042767628488935
48-49	0.18786299377600196
50-51	0.18639272319978634
52-53	0.19090302692231245
54-55	0.18989705231753334
56-57	0.20002312636124395
58-59	0.19637508658567135
60-61	0.2016592169272583
62-63	0.19888449576462583
64-65	0.20007839969117683
66-67	0.19015130963522475
68-69	0.18881369505084816
70-71	0.17344770932949694
72-73	0.18032371157315194
74-75	0.1800694542554605
76-77	0.17816805170576813
78-79	0.2052409287069114
80-81	0.2139409508383527
82-83	0.21151997898729089
84-85	0.20642377796747582
86-87	0.22208823967046482
88-89	0.2214360143772564
90-91	0.22036371177655778
92-93	0.22452026618751322
94-95	0.23771953737549406
96-97	0.2374210613938563
98-99	0.22624479408142026
100-101	0.2371668040761649
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4522977.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.684433520626264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47616995698823	39.23734824969809
2	13.280577465227802	13.993594011646426
3	4.731646803921381	7.47852394452839
4	2.299052706803113	4.844971579679378
5	1.3267294593340555	3.4948995000070733
6	0.8081755774836454	2.5546963490958516
7	0.5526605912034793	2.0381627123710797
8	0.39677775174966656	1.6723208867615087
9	0.29506068832785576	1.399059469688323
>10	1.6989442684928624	16.128136992585805
>50	0.09982056013088696	3.5766791727091256
>100	0.032826922585795976	2.788764185829856
>500	9.259313056701564E-4	0.3334482383916122
>1k	6.313164455396526E-4	0.45939470700759927
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.210933197316723E-5
2	0.0	0.0	0.0	0.0	2.210933197316723E-5
3	0.0	0.0	0.0	0.0	2.210933197316723E-5
4	0.0	0.0	0.0	0.0	2.210933197316723E-5
5	2.210933197316723E-5	0.0	0.0	0.0	4.421866394633446E-5
6	2.210933197316723E-5	0.0	0.0	0.0	1.326559918390034E-4
7	2.210933197316723E-5	0.0	0.0	0.0	1.326559918390034E-4
8	2.210933197316723E-5	0.0	0.0	0.0	1.547653238121706E-4
9	2.210933197316723E-5	0.0	0.0	0.0	1.9898398775850508E-4
10-11	2.210933197316723E-5	0.0	0.0	0.0	1.9898398775850508E-4
12-13	2.210933197316723E-5	0.0	0.0	0.0	3.5374931157067566E-4
14-15	2.210933197316723E-5	0.0	0.0	2.210933197316723E-5	4.090226415035937E-4
16-17	2.210933197316723E-5	0.0	0.0	2.210933197316723E-5	4.6429597143651184E-4
18-19	2.210933197316723E-5	0.0	0.0	4.4218663946334465E-5	4.864053034096791E-4
20-21	2.210933197316723E-5	0.0	0.0	6.63279959195017E-5	5.306239673560136E-4
22-23	2.210933197316723E-5	0.0	0.0	1.2160132585241977E-4	5.74842631302348E-4
24-25	2.210933197316723E-5	0.0	0.0	1.6581998979875424E-4	6.411706272218497E-4
26-27	2.210933197316723E-5	0.0	0.0	3.3163997959750847E-4	7.074986231413514E-4
28-29	2.210933197316723E-5	0.0	0.0	0.0017134732279204604	7.296079551145186E-4
30-31	2.210933197316723E-5	0.0	0.0	0.007296079551145186	7.406626211011022E-4
32-33	2.210933197316723E-5	0.0	0.0	0.01984312544591759	7.738266190608531E-4
34-35	2.210933197316723E-5	0.0	0.0	0.03819387098364639	8.622639469535219E-4
36-37	2.210933197316723E-5	0.0	0.0	0.06761033717394539	9.064826108998564E-4
38-39	2.210933197316723E-5	0.0	0.0	0.11831809005440444	9.285919428730237E-4
40-41	2.210933197316723E-5	0.0	0.0	0.17673094512751225	0.001005974604779109
42-43	2.210933197316723E-5	0.0	0.0	0.23766426404556112	0.0010280839367522764
44-45	2.210933197316723E-5	0.0	0.0	0.314262044666599	0.0010501932687254434
46-47	2.210933197316723E-5	0.0	0.0	0.40341792584839586	0.0010723026006986107
48-49	2.210933197316723E-5	0.0	0.0	0.503849566336508	0.001149685262604696
50-51	2.210933197316723E-5	0.0	0.0	0.6126385343104773	0.0011717945945778632
52-53	2.210933197316723E-5	0.0	0.0	0.7231409755123672	0.0012491772564839484
54-55	2.210933197316723E-5	0.0	0.0	0.8531106835166307	0.0013044505864168666
56-57	2.210933197316723E-5	0.0	0.0	1.006206752764827	0.0013044505864168666
58-59	2.210933197316723E-5	0.0	0.0	1.1734859584738104	0.0013265599183900339
60-61	4.421866394633446E-5	0.0	0.0	1.3534890847333516	0.0013486692503632011
62-63	4.421866394633446E-5	0.0	0.0	1.5406114158882525	0.0013707785823363684
64-65	4.421866394633446E-5	0.0	0.0	1.7428786394447728	0.0013928879143095354
66-67	4.421866394633446E-5	0.0	0.0	1.9836382099665775	0.0014149972462827027
68-69	4.421866394633446E-5	0.0	0.0	2.251338001497686	0.0014149972462827027
70-71	4.421866394633446E-5	0.0	0.0	2.538173419851571	0.0014702705762156209
72-73	4.421866394633446E-5	0.0	0.0	2.8397889266295184	0.0016471452320009588
74-75	4.421866394633446E-5	0.0	0.0	3.1537967139784264	0.0016581998979875424
76-77	4.421866394633446E-5	0.0	0.0	3.5061089189708463	0.0016581998979875424
78-79	4.421866394633446E-5	0.0	0.0	3.90490599443685	0.0016581998979875424
80-81	4.421866394633446E-5	0.0	0.0	4.322451783416099	0.0016913638959472931
82-83	4.421866394633446E-5	0.0	0.0	4.755938400748003	0.0017466372258802111
84-85	4.421866394633446E-5	4.421866394633446E-5	0.0	5.214298016549719	0.0017466372258802111
86-87	5.5273329932918074E-5	4.421866394633446E-5	0.0	5.706175379622757	0.0018129652217997129
88-89	6.63279959195017E-5	4.421866394633446E-5	0.0	6.244648159829245	0.0019124572156789654
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3365	0.0	41.18085	1
GTATCAA	5380	0.0	40.488148	1
TCAACGC	6390	0.0	33.27204	4
CAACGCA	6460	0.0	32.834778	5
ATCAACG	6860	0.0	30.923292	3
AACGCAG	6985	0.0	30.493307	6
TATCAAC	7100	0.0	30.279716	2
GTACATG	13010	0.0	30.057125	1
TACATGG	13765	0.0	27.719906	2
ACATGGG	14325	0.0	26.072483	3
CATGGGG	12180	0.0	23.57276	4
ACGCAGA	8720	0.0	23.505457	7
CGCAGAG	8965	0.0	22.813194	8
GAGTACA	9715	0.0	21.59121	1
AGTACAT	9730	0.0	19.949057	2
ATGGGGG	8005	0.0	19.502651	5
GTATAGC	1365	0.0	17.731073	1
GCAGAGT	11040	0.0	17.106972	9
GTGTAGC	2510	0.0	17.016346	1
AGAGTAC	8750	0.0	16.757511	10-11
>>END_MODULE