FastQCFastQC Report
Mon 15 Aug 2016
SRR2921369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4522977
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33400.042.6869851
GTATCAA50550.039.204471
TCAACGC62850.031.033284
CAACGCA64800.030.1517545
ATCAACG68050.028.7316273
TATCAAC68600.028.501272
GTACATG125800.028.2580151
AACGCAG71250.027.5535346
TACATGG136250.025.8439272
ACATGGG139850.024.4660533
CATGGGG118650.021.798164
ACGCAGA90700.021.2787
CGCAGAG94700.019.9776868
GAGTACA95800.019.0496251
ATGGGGG74950.018.9012435
GTATAGC12900.018.7888931
AGTACAT99300.016.965642
AGAGTAC90500.016.02003110-11
GCAGAGT114450.015.6165159
TAAGGTG21000.015.1670565
Produced by FastQC (version 0.11.5)