##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921369_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4522977
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.415655883282184	33.0	31.0	34.0	29.0	34.0
2	31.5431409445593	34.0	31.0	34.0	29.0	34.0
3	31.625692105000756	34.0	31.0	34.0	28.0	34.0
4	35.11299150979543	37.0	35.0	37.0	33.0	37.0
5	34.85081684032441	37.0	35.0	37.0	32.0	37.0
6	34.80748453949688	37.0	35.0	37.0	32.0	37.0
7	34.66723996164473	37.0	35.0	37.0	32.0	37.0
8	34.63269214059678	37.0	35.0	37.0	32.0	37.0
9	36.25963718144045	39.0	37.0	39.0	32.0	39.0
10-11	36.10789221346914	39.0	37.0	39.0	32.0	39.0
12-13	36.0340827512499	39.0	37.0	39.0	31.0	39.0
14-15	37.28956415210601	40.0	38.0	41.0	31.0	41.0
16-17	37.26082611076731	40.0	38.0	41.0	31.0	41.0
18-19	37.20938255931878	40.0	38.0	41.0	31.0	41.0
20-21	37.16762974916742	40.0	38.0	41.0	31.0	41.0
22-23	37.05544578272231	40.0	38.0	41.0	31.0	41.0
24-25	36.98950757432549	40.0	37.0	41.0	31.0	41.0
26-27	36.85433133973487	40.0	37.0	41.0	30.0	41.0
28-29	36.706431074047025	40.0	37.0	41.0	30.0	41.0
30-31	36.57725520160726	40.0	37.0	41.0	30.0	41.0
32-33	36.54774134380962	40.0	37.0	41.0	30.0	41.0
34-35	36.69680721347909	40.0	37.0	41.0	30.0	41.0
36-37	36.68413193345887	40.0	37.0	41.0	30.0	41.0
38-39	36.60719532732534	40.0	37.0	41.0	30.0	41.0
40-41	36.48967416814192	40.0	37.0	41.0	30.0	41.0
42-43	36.39586327765983	40.0	37.0	41.0	30.0	41.0
44-45	36.27361160580742	40.0	36.0	41.0	29.0	41.0
46-47	36.133819274340766	40.0	36.0	41.0	28.5	41.0
48-49	35.98451672427253	40.0	36.0	41.0	28.0	41.0
50-51	35.825932234455315	40.0	35.0	41.0	28.0	41.0
52-53	35.65165730447004	39.0	35.0	41.0	28.0	41.0
54-55	35.433795484699566	39.0	35.0	41.0	27.0	41.0
56-57	35.16375774185896	39.0	35.0	41.0	26.5	41.0
58-59	34.925848174775155	38.5	35.0	40.0	26.0	41.0
60-61	34.635359742046	38.0	34.5	40.0	26.0	41.0
62-63	34.3246944435048	37.0	34.0	40.0	26.0	41.0
64-65	33.9999338930974	37.0	34.0	40.0	26.0	41.0
66-67	33.6541779894083	36.0	34.0	39.0	25.0	41.0
68-69	33.286061702281486	36.0	34.0	39.0	24.0	40.5
70-71	32.9057255873731	35.0	33.0	38.5	23.5	40.0
72-73	32.513007030546476	35.0	33.0	37.0	22.5	39.5
74-75	32.11134082707031	35.0	33.0	37.0	21.5	39.0
76-77	29.380890395860956	33.0	29.0	34.5	16.5	36.5
78-79	31.04393433793716	34.5	31.5	35.5	19.5	37.0
80-81	31.25346127119373	35.0	32.0	35.0	20.0	37.0
82-83	31.10834324384139	35.0	32.0	35.0	20.0	37.0
84-85	30.8950086414324	35.0	32.0	35.0	18.5	36.0
86-87	30.670305531069474	35.0	32.0	35.0	17.5	36.0
88-89	30.407158272084956	35.0	32.0	35.0	11.0	35.5
90-91	30.23586976011596	35.0	32.0	35.0	8.5	35.0
92-93	30.10099233314695	34.0	32.0	35.0	4.5	35.0
94-95	30.01445463905742	34.0	32.0	35.0	2.0	35.0
96-97	29.837816221484214	34.0	31.0	35.0	2.0	35.0
98-99	29.584312058186455	34.0	31.0	35.0	2.0	35.0
100-101	28.50072562827536	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84091.0
3	29150.0
4	16085.0
5	11024.0
6	9765.0
7	9648.0
8	9965.0
9	10673.0
10	11610.0
11	12619.0
12	13036.0
13	14091.0
14	15166.0
15	15907.0
16	16976.0
17	17505.0
18	17060.0
19	15884.0
20	15539.0
21	15352.0
22	16382.0
23	18751.0
24	22019.0
25	26571.0
26	32076.0
27	39166.0
28	47757.0
29	57226.0
30	69547.0
31	87308.0
32	111210.0
33	148484.0
34	215473.0
35	322876.0
36	575254.0
37	1132551.0
38	1113661.0
39	125514.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.038244907520784	18.480411020718872	12.732006219159386	26.749337852600963
2	15.453687004927975	19.911173076527657	37.1951245126801	27.440015405864266
3	18.445365674507933	23.4588565185773	28.29245470125458	29.803323105660184
4	13.291185000653364	15.629780684657609	34.102075349173084	36.97695896551594
5	14.527628918143812	37.072115784389524	33.84909584358739	14.551159453879265
6	35.66640577326152	34.115549246946664	16.04883570790551	14.169209271886304
7	30.364289313373888	31.00488594775425	19.85414012161475	18.776684617257107
8	27.784195716344755	34.89890254355987	18.639690836414495	18.67721090368088
9	27.111353232915675	15.13614045265772	18.288268608986648	39.464237705439956
10-11	26.606097867034578	24.82355862501212	26.895862297604207	21.674481210349104
12-13	26.765119783151096	21.908206231973935	27.002386708718728	24.32428727615624
14-15	23.806174069095526	23.61268510217869	23.891222099177302	28.68991872954848
16-17	24.472837799519894	26.769383153869665	24.72068393151178	24.037095115098662
18-19	24.46864195756653	26.249443810334487	25.78411826319586	23.497795968903127
20-21	24.841726376136776	25.330445832552588	25.915341616657738	23.912486174652894
22-23	24.6229463687971	25.102090133814905	25.678868989450283	24.596094507937714
24-25	24.530909164876146	25.356275998264827	25.568717225106557	24.544097611752466
26-27	24.294134886370532	25.5981727396071	25.647467026499854	24.460225347522513
28-29	24.444839983549503	25.862843990041256	25.41800621757992	24.274309808829315
30-31	24.783217182174962	25.48624937752473	25.312840813503314	24.417692626796995
32-33	24.421756748539174	25.505702329026875	25.370427495010805	24.702113427423143
34-35	24.693005760778547	25.567747246587547	25.514789565634246	24.22445742699966
36-37	24.712325059996882	25.487030735626035	25.54774341090045	24.252900793476638
38-39	24.66137279768506	25.692443545919406	25.401428884750015	24.24475477164552
40-41	24.77963276420493	25.42217545602113	25.496853728645803	24.30133805112814
42-43	24.496257830022333	25.420421301884005	25.344535355012788	24.738785513080874
44-45	24.441176151925703	25.43335340300302	25.554523204569783	24.570947240501493
46-47	24.60807197058981	25.424907474028824	25.3639015821731	24.603118973208264
48-49	24.39476501171829	25.608220117693705	25.428647999509995	24.56836687107801
50-51	24.44477094517872	25.597587550195342	25.525838461071586	24.43180304355435
52-53	24.742665331831056	25.356883542078272	25.430630409135368	24.4698207169553
54-55	24.171027864536207	25.309646799075512	25.7527882058013	24.766537130586983
56-57	24.30051615276919	25.476674069337463	25.556814392843908	24.665995385049435
58-59	24.44043540417903	25.46380570369878	25.53910603438564	24.556652857736548
60-61	24.491334885448154	25.495006076641186	25.659456249786487	24.354202788124166
62-63	24.47994282361896	25.456768654358207	25.605392748179128	24.457895773843706
64-65	24.213798434353542	25.296359247189233	25.628597431578992	24.86124488687823
66-67	24.230634357502762	25.475044613447988	25.510178014762662	24.784143014286588
68-69	24.270577545853353	25.338475704089507	25.593983519099062	24.796963230958077
70-71	24.16438764596598	25.213771201693913	25.592769330591096	25.029071821749007
72-73	24.183289117863787	25.48528283894276	25.38682638491431	24.944601658279144
74-75	24.06774904466952	25.488667340651183	25.544661941993173	24.89892167268612
76-77	24.112781801588813	25.165613768215188	25.558927076310873	25.162677353885133
78-79	23.986207804404806	25.171708299709234	25.69424755486651	25.14783634101945
80-81	24.15909406134756	25.304192766798707	25.47058767576043	25.066125496093306
82-83	24.107631237724735	25.352685611829056	25.56447001985714	24.97521313058907
84-85	24.026188295106866	25.340391218786785	25.481072691251388	25.15234779485496
86-87	23.82883656833634	25.49693356875111	25.482285417023697	25.191944445888854
88-89	23.691199202315556	25.29300782499534	25.55932013273164	25.456472839957467
90-91	23.79333709643256	25.163212415122338	25.649129071715876	25.39432141672923
92-93	23.90506353264395	25.06122105887187	25.65721184128812	25.376503567196064
94-95	23.755258546419643	25.012754942699218	25.65390155317891	25.578084957702234
96-97	23.486725023855453	24.86924137165522	25.80348190694609	25.840551697543233
98-99	23.348143801569204	24.8795636100251	25.72818442570486	26.044108162700834
100-101	23.387770226842743	24.941116488422395	25.759980348947682	25.91113293578718
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	29.0
2	34.5
3	60.5
4	61.0
5	66.5
6	123.0
7	261.5
8	372.0
9	388.0
10	353.5
11	308.0
12	296.5
13	311.5
14	372.5
15	413.0
16	479.5
17	531.0
18	547.0
19	620.5
20	720.5
21	857.0
22	974.0
23	1167.0
24	1482.5
25	1924.5
26	2694.0
27	3802.5
28	5470.5
29	8343.0
30	12509.5
31	18080.0
32	26682.0
33	34245.5
34	40897.0
35	50553.5
36	63132.0
37	76062.0
38	87730.5
39	99250.0
40	109639.0
41	119684.0
42	130012.5
43	141310.0
44	152920.0
45	165450.5
46	175774.5
47	184152.0
48	194384.5
49	201545.5
50	205045.5
51	208072.5
52	209814.0
53	210582.5
54	209320.0
55	201815.0
56	189844.0
57	174079.5
58	156876.0
59	137729.0
60	115476.5
61	95838.0
62	77974.0
63	58848.5
64	41711.5
65	30039.0
66	22186.5
67	16717.5
68	12486.5
69	9085.5
70	6617.5
71	4744.5
72	3286.0
73	2430.5
74	1850.5
75	1309.0
76	866.5
77	513.5
78	285.5
79	154.0
80	85.5
81	56.5
82	39.5
83	29.0
84	23.0
85	15.0
86	10.5
87	5.5
88	3.0
89	1.5
90	2.0
91	2.5
92	1.5
93	1.5
94	1.5
95	1.0
96	1.0
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14616479367460855
2	5.306239673560136E-4
3	0.0061906129524868245
4	0.004864053034096791
5	0.02637643304398851
6	0.0012602319224705321
7	1.326559918390034E-4
8	0.0014149972462827027
9	2.210933197316723E-5
10-11	0.09902769790781603
12-13	2.653119836780068E-4
14-15	0.005273075675600385
16-17	2.100386537450887E-4
18-19	3.2058531361092485E-4
20-21	7.627719530742694E-4
22-23	2.87421315651174E-4
24-25	0.0017466372258802111
26-27	0.003106361142229996
28-29	0.0070086582354940115
30-31	0.015675516368975566
32-33	0.015520751045163397
34-35	0.011010447322637281
36-37	7.406626211011022E-4
38-39	1.9898398775850508E-4
40-41	0.005361513003493053
42-43	0.008854787455253475
44-45	0.008799514125320557
46-47	0.010236620703576428
48-49	0.012381225904973649
50-51	0.006002683630714903
52-53	0.0016139812340412079
54-55	9.1753727688644E-4
56-57	0.006301159612352661
58-59	0.00929697409471682
60-61	0.007307134217131769
62-63	0.0183507455377288
64-65	0.033650403263160523
66-67	0.03622614043803451
68-69	0.02647592503786776
70-71	0.04082488148845329
72-73	0.0261111210603105
74-75	0.024596631820148544
76-77	0.2359286814856675
78-79	0.06698022121271012
80-81	0.053195052727440365
82-83	0.02046218674116627
84-85	0.031284704742031634
86-87	0.08037847638844947
88-89	0.2635432371201534
90-91	0.31576547924077436
92-93	0.05017712891310303
94-95	0.004875107700083374
96-97	0.009429630086555823
98-99	0.0011054665986583614
100-101	0.0014149972462827027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4522977.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.84642824155126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.79993511617616	38.26263040231966
2	13.445999636054184	13.94254110533215
3	4.9700694019417595	7.7304103980990915
4	2.3661829324492714	4.907125344544579
5	1.372154765817775	3.557066180113693
6	0.8591242268122289	2.6725513545599062
7	0.569867328539984	2.068190988944696
8	0.4104802820708551	1.702554919116665
9	0.3182591218287586	1.4850538849902466
>10	1.748267465072963	16.35998817159988
>50	0.10262409459463032	3.615023630031948
>100	0.035494746581811475	2.9224041685615054
>500	9.416502720936146E-4	0.35308822807328044
>1k	5.992317874893011E-4	0.42137122371269237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1054665986583615E-4
2	0.0	0.0	0.0	0.0	1.1054665986583615E-4
3	0.0	0.0	0.0	0.0	1.1054665986583615E-4
4	0.0	0.0	0.0	0.0	1.1054665986583615E-4
5	0.0	0.0	0.0	0.0	1.1054665986583615E-4
6	0.0	0.0	0.0	0.0	1.326559918390034E-4
7	0.0	0.0	0.0	2.210933197316723E-5	1.326559918390034E-4
8	0.0	0.0	0.0	2.210933197316723E-5	1.547653238121706E-4
9	0.0	0.0	0.0	2.210933197316723E-5	1.547653238121706E-4
10-11	0.0	0.0	0.0	2.210933197316723E-5	1.547653238121706E-4
12-13	0.0	0.0	0.0	3.316399795975084E-5	3.6480397755725934E-4
14-15	0.0	0.0	0.0	4.421866394633446E-5	5.085146353828463E-4
16-17	0.0	0.0	0.0	4.421866394633446E-5	5.527332993291807E-4
18-19	0.0	0.0	0.0	4.421866394633446E-5	5.969519632755152E-4
20-21	0.0	0.0	0.0	4.421866394633446E-5	6.190612952486825E-4
22-23	0.0	0.0	0.0	7.73826619060853E-5	6.743346251816005E-4
24-25	0.0	0.0	0.0	1.43710657825587E-4	7.517172870876858E-4
26-27	0.0	0.0	0.0	2.984759816377576E-4	8.512092809669384E-4
28-29	0.0	0.0	0.0	0.0016692545639741259	8.733186129401056E-4
30-31	0.0	0.0	0.0	0.0073181888831183525	8.954279449132728E-4
32-33	0.0	0.0	0.0	0.020218984089461434	9.507012748461909E-4
34-35	0.0	0.0	0.0	0.038459182967324396	0.0010170292707656927
36-37	0.0	0.0	0.0	0.06776510249775757	0.0010170292707656927
38-39	0.0	0.0	0.0	0.11830703538841786	0.0010612479347120272
40-41	0.0	0.0	0.0	0.17627770382206231	0.0010612479347120272
42-43	0.0	0.0	0.0	0.23743211605984288	0.0010833572666851944
44-45	0.0	0.0	0.0	0.3138088033611491	0.001149685262604696
46-47	0.0	0.0	0.0	0.40296468454294593	0.001238122590497365
48-49	0.0	0.0	0.0	0.502390350426279	0.0013155052524034504
50-51	0.0	0.0	0.0	0.6106044757689459	0.0013265599183900339
52-53	0.0	0.0	0.0	0.7207310583272919	0.0013707785823363684
54-55	0.0	0.0	0.0	0.8497942837206557	0.0014149972462827027
56-57	0.0	0.0	0.0	1.001696449042301	0.0014260519122692864
58-59	0.0	0.0	0.0	1.1679696801465052	0.00143710657825587
60-61	0.0	0.0	0.0	1.34705526912916	0.00143710657825587
62-63	0.0	0.0	0.0	1.5335253749908522	0.0014813252422022044
64-65	0.0	0.0	0.0	1.7350187719283117	0.001492379908188788
66-67	0.0	0.0	0.0	1.9742306892119947	0.0015144892401619554
68-69	0.0	0.0	0.0	2.2399627501974915	0.0015255439061485389
70-71	0.0	0.0	0.0	2.525537936628906	0.0015587079041082899
72-73	2.210933197316723E-5	0.0	0.0	2.8271313340748803	0.0017245278939070439
74-75	2.210933197316723E-5	0.0	0.0	3.141725018721077	0.0017245278939070439
76-77	2.210933197316723E-5	0.0	0.0	3.494368863693094	0.0017245278939070439
78-79	2.210933197316723E-5	0.0	0.0	3.8948241390570857	0.0017245278939070439
80-81	2.210933197316723E-5	0.0	0.0	4.314249221254054	0.0017466372258802111
82-83	2.210933197316723E-5	0.0	0.0	4.751240167703704	0.00183507455377288
84-85	2.210933197316723E-5	0.0	0.0	5.212684035315679	0.0018571838857460474
86-87	2.210933197316723E-5	0.0	0.0	5.706672839592153	0.0019456212136387163
88-89	2.210933197316723E-5	0.0	0.0	6.247201787672147	0.0020340585415313853
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3340	0.0	42.686985	1
GTATCAA	5055	0.0	39.20447	1
TCAACGC	6285	0.0	31.03328	4
CAACGCA	6480	0.0	30.151754	5
ATCAACG	6805	0.0	28.731627	3
TATCAAC	6860	0.0	28.50127	2
GTACATG	12580	0.0	28.258015	1
AACGCAG	7125	0.0	27.553534	6
TACATGG	13625	0.0	25.843927	2
ACATGGG	13985	0.0	24.466053	3
CATGGGG	11865	0.0	21.79816	4
ACGCAGA	9070	0.0	21.278	7
CGCAGAG	9470	0.0	19.977686	8
GAGTACA	9580	0.0	19.049625	1
ATGGGGG	7495	0.0	18.901243	5
GTATAGC	1290	0.0	18.788893	1
AGTACAT	9930	0.0	16.96564	2
AGAGTAC	9050	0.0	16.020031	10-11
GCAGAGT	11445	0.0	15.616515	9
TAAGGTG	2100	0.0	15.167056	5
>>END_MODULE