FastQCFastQC Report
Mon 15 Aug 2016
SRR2921368_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921368_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4082474
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27300.042.5876461
GTATCAA45450.039.2585451
TCAACGC49750.034.6295624
CAACGCA52650.032.720145
ATCAACG54650.031.4335423
TATCAAC55350.031.38012
AACGCAG56100.030.8703146
GTACATG110150.028.5202751
TACATGG113650.026.6822412
ACATGGG117150.025.5600663
ACGCAGA72350.023.1523517
CATGGGG104750.022.3823854
CGCAGAG75350.021.4764258
TACACCG6450.021.3375785
GAGTACA80750.020.4511131
GTATAGG9500.019.9809721
GTATATA10550.019.341771
AGTACAT81500.018.4583362
ATGGGGG66000.018.264035
ATACCGT3852.5465852E-1017.2535466
Produced by FastQC (version 0.11.5)