##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921368_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4082474
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.316792709518786	31.0	31.0	34.0	26.0	34.0
2	30.359770570492305	31.0	31.0	34.0	26.0	34.0
3	30.41822189192142	33.0	31.0	34.0	26.0	34.0
4	33.86508768947457	37.0	35.0	37.0	30.0	37.0
5	33.85541169398752	37.0	35.0	37.0	30.0	37.0
6	33.901266731888555	37.0	35.0	37.0	30.0	37.0
7	33.80939229496624	37.0	35.0	37.0	29.0	37.0
8	33.77375434601665	37.0	35.0	37.0	29.0	37.0
9	35.326932639375045	39.0	37.0	39.0	29.0	39.0
10-11	35.22006876222604	39.0	35.0	39.0	29.0	39.0
12-13	35.10028379850061	39.0	35.0	39.0	28.0	39.0
14-15	36.24108567500981	40.0	37.0	41.0	27.0	41.0
16-17	36.188727350131316	40.0	36.5	41.0	27.0	41.0
18-19	36.12558549056283	40.0	36.5	41.0	27.0	41.0
20-21	36.05462018864051	40.0	36.0	41.0	27.0	41.0
22-23	35.939504207497706	40.0	36.0	41.0	27.0	41.0
24-25	35.83969597846796	40.0	36.0	41.0	27.0	41.0
26-27	35.69382021783849	40.0	36.0	41.0	26.5	41.0
28-29	35.55646698546029	40.0	36.0	41.0	25.5	41.0
30-31	35.422127121936356	40.0	36.0	41.0	25.0	41.0
32-33	35.256600042033334	39.5	35.0	41.0	25.0	41.0
34-35	35.099127146921205	39.0	35.0	41.0	24.0	41.0
36-37	34.93621563787056	39.0	35.0	41.0	24.0	41.0
38-39	35.18641061767938	39.5	35.0	41.0	24.5	41.0
40-41	35.31884795837034	40.0	35.0	41.0	24.0	41.0
42-43	35.16432217327042	40.0	35.0	41.0	24.0	41.0
44-45	35.05706062059428	40.0	35.0	41.0	23.0	41.0
46-47	34.91835147021145	40.0	35.0	41.0	23.0	41.0
48-49	34.77630500524927	39.0	35.0	41.0	22.0	41.0
50-51	33.05022652930552	37.0	32.5	39.5	18.0	40.0
52-53	33.2024773458447	37.5	33.0	39.5	18.0	40.0
54-55	34.06040356901232	39.0	34.0	40.5	18.5	41.0
56-57	34.06499943416664	38.5	34.0	41.0	18.0	41.0
58-59	33.873722649550245	38.0	34.0	40.5	18.0	41.0
60-61	33.616231113780515	37.5	34.0	40.0	16.0	41.0
62-63	33.319905405398785	37.0	33.5	40.0	13.0	41.0
64-65	33.01738149465251	36.5	33.0	40.0	12.0	41.0
66-67	32.669221040966825	36.0	33.0	39.0	8.0	41.0
68-69	32.30142127053351	35.5	33.0	39.0	7.0	41.0
70-71	31.92073409408119	35.0	32.0	38.5	4.0	40.0
72-73	31.57101845596567	35.0	32.0	37.0	2.0	39.5
74-75	31.181285906536086	35.0	32.0	37.0	2.0	39.0
76-77	30.817067420392632	35.0	32.0	36.0	2.0	39.0
78-79	30.490255051226292	35.0	31.0	36.0	2.0	37.5
80-81	30.162604219892152	35.0	31.0	35.5	2.0	37.0
82-83	29.881788469442796	35.0	31.0	35.0	2.0	37.0
84-85	29.663228963613733	35.0	31.0	35.0	2.0	36.0
86-87	29.455864385174284	35.0	31.0	35.0	2.0	36.0
88-89	29.272153111079213	35.0	31.0	35.0	2.0	36.0
90-91	29.037387623289213	34.5	30.0	35.0	2.0	35.0
92-93	28.85589057027675	34.0	30.0	35.0	2.0	35.0
94-95	28.658569901486207	34.0	30.0	35.0	2.0	35.0
96-97	28.414009005323734	34.0	30.0	35.0	2.0	35.0
98-99	28.19431109665365	34.0	30.0	35.0	2.0	35.0
100-101	27.157093468323374	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	151497.0
3	25530.0
4	17225.0
5	13089.0
6	11875.0
7	11918.0
8	12482.0
9	13482.0
10	14345.0
11	15404.0
12	15262.0
13	15112.0
14	15672.0
15	16530.0
16	16290.0
17	16568.0
18	16418.0
19	15659.0
20	16184.0
21	17148.0
22	18650.0
23	21388.0
24	25309.0
25	30266.0
26	35951.0
27	43054.0
28	50926.0
29	60192.0
30	72546.0
31	89061.0
32	110852.0
33	144016.0
34	201761.0
35	294180.0
36	498991.0
37	901450.0
38	893363.0
39	142812.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.69915174798046	16.800526600970613	11.88952162585599	25.610800025192937
2	16.575572342846666	18.436264078037294	38.601706826841706	26.386456752274334
3	20.281191521564175	22.029503764675667	28.056317705101552	29.632987008658606
4	13.61896951967908	14.248118752867455	34.82336471686202	37.30954701059145
5	14.904803308529816	36.51280545723133	33.722262684641784	14.860128549597068
6	36.054304606557416	33.41088101053704	15.993669444042933	14.541144938862608
7	30.958024317531667	30.48292821671026	19.579309788968942	18.97973767678913
8	27.89191162419212	34.56542191167319	18.5920924329807	18.95057403115399
9	27.040223084284285	15.392585318909882	18.267892238648443	39.29929935815739
10-11	26.773999088841844	24.577284721734774	26.71053161876839	21.938184570654997
12-13	26.961865375413247	21.69043827756542	26.87750725300993	24.470189094011406
14-15	23.811092176349128	23.472729286652854	23.720212670541514	28.99596586645651
16-17	24.580083336477767	26.657933308275872	24.60018729390687	24.161796061339487
18-19	24.6148506909412	26.04847834172152	25.72145668760814	23.61521427972914
20-21	24.89046030072546	25.267365561350687	25.85280236154434	23.98937177637951
22-23	24.74707416948049	24.982236039928996	25.646563422698193	24.62412636789232
24-25	24.5650061942868	25.256614764371438	25.568785744575816	24.609593296765947
26-27	24.413280476447053	25.478087498091867	25.58227231195462	24.526359713506462
28-29	24.54762157637596	25.65400784957645	25.40774542666438	24.390625147383215
30-31	24.765145055610276	25.363207077428605	25.36492195060945	24.50672591635167
32-33	24.489209979571584	25.369501028770042	25.376654281577014	24.76463471008136
34-35	24.723124881340986	25.46049496885775	25.44514738215246	24.371232767648806
36-37	24.818403834989628	25.36518869981212	25.46652590014232	24.349881565055938
38-39	24.634797653905785	25.55171992281592	25.384762929888844	24.428719493389455
40-41	24.74140537953238	25.303808031072627	25.533065682353833	24.421720907041156
42-43	24.567660007616443	25.277476789542273	25.4208220531779	24.734041149663376
44-45	24.447370881431205	25.389259335512577	25.57612541106242	24.587244371993794
46-47	24.65196213646461	25.363375874021187	25.401254624716923	24.583407364797278
48-49	24.51689326025438	25.458793578871514	25.37462083923166	24.649692321642444
50-51	24.48029549880638	25.51756953605981	25.56911544713532	24.433019517998492
52-53	24.80941793863507	25.29813897278149	25.49079781325283	24.4016452753306
54-55	24.29153602861636	25.174999361943147	25.810638959947703	24.722825649492794
56-57	24.408238948907545	25.402574962985597	25.644363533281673	24.54482255482519
58-59	24.498496169557956	25.319105334395196	25.642020863423237	24.54037763262361
60-61	24.50274935917008	25.364212778114297	25.741777202260746	24.39126066045488
62-63	24.512600859397164	25.34678010761608	25.74991090896418	24.390708124022574
64-65	24.31737358994028	25.188857774777496	25.743095013202467	24.75067362207976
66-67	24.332204578604166	25.34295480477844	25.61950523510774	24.70533538150965
68-69	24.430679979042456	25.23001149720055	25.71570380168372	24.623604722073274
70-71	24.3257769644128	25.096242213732246	25.728921516727475	24.849059305127476
72-73	24.377397715075993	25.257862493294862	25.588996852696066	24.775742938933078
74-75	24.263472560186717	25.425301554712338	25.703149164512507	24.60807672058844
76-77	24.368885464489992	25.128774847681047	25.683699234172447	24.818640453656514
78-79	24.28516010855	25.149208388338323	25.750128581900256	24.81550292121142
80-81	24.408117989425588	25.324011531483237	25.576890198693363	24.690980280397817
82-83	24.436618655228916	25.363424030150263	25.611472965906067	24.588484348714758
84-85	24.419002929269116	25.40067441055347	25.5244893844215	24.655833275755914
86-87	24.305330952276037	25.50841215399724	25.569943394450114	24.61631349927661
88-89	24.17461739126059	25.326822435447145	25.64006038422464	24.85849978906763
90-91	24.31918516278284	25.233709441758347	25.688547464091155	24.75855793136766
92-93	24.37571216236768	25.188075920942627	25.75983113949784	24.676380777191852
94-95	24.384225930851816	25.041818450106224	25.82639469103942	24.74756092800254
96-97	24.28048501430903	24.98141397197905	25.86871880640872	24.869382207303204
98-99	24.152287097765683	24.98638719959778	25.82539723845502	25.035928464181517
100-101	24.413134243635145	24.894600907470135	25.904391916319568	24.787872932575148
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	23.5
2	45.0
3	71.0
4	69.5
5	85.5
6	154.0
7	304.5
8	470.0
9	551.5
10	506.5
11	429.0
12	454.0
13	521.5
14	599.0
15	714.0
16	824.0
17	900.0
18	973.0
19	1109.0
20	1207.0
21	1293.0
22	1462.0
23	1617.5
24	1812.0
25	2095.5
26	2571.0
27	3410.0
28	4662.5
29	6832.5
30	10332.0
31	15437.0
32	23082.5
33	29441.0
34	34934.5
35	43068.5
36	54234.0
37	65978.0
38	77003.0
39	87970.5
40	97559.5
41	107149.5
42	117322.5
43	128230.0
44	139295.0
45	151877.5
46	161718.0
47	168879.0
48	176489.5
49	181795.5
50	184619.0
51	186895.5
52	189106.5
53	189326.5
54	187436.0
55	180984.0
56	169230.5
57	154551.0
58	139989.5
59	123237.0
60	103519.0
61	86484.5
62	71441.0
63	55049.5
64	39836.0
65	28812.5
66	21391.0
67	16286.5
68	12287.0
69	9178.0
70	6855.5
71	4978.5
72	3483.0
73	2536.5
74	2000.0
75	1476.5
76	1028.0
77	709.0
78	451.5
79	305.0
80	232.0
81	185.5
82	146.5
83	126.0
84	110.0
85	89.5
86	73.5
87	64.5
88	65.5
89	62.5
90	52.5
91	39.0
92	27.5
93	22.5
94	23.5
95	26.5
96	20.5
97	12.5
98	16.5
99	12.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04813257843160789
2	0.055628033393476606
3	0.046785356134539983
4	0.05393788178442777
5	0.045805558100308785
6	0.016754546385353584
7	0.013790657331804195
8	0.019938889996605
9	0.019302021274354715
10-11	0.020955430457119874
12-13	0.026087122661405806
14-15	0.04399293173698105
16-17	0.02910000161666676
18-19	0.023025253804433293
20-21	0.026381062071675166
22-23	0.04606275508429447
24-25	0.04158017907768671
26-27	0.030986112832561825
28-29	0.020881945604552532
30-31	0.013215025986693362
32-33	0.010165404605148741
34-35	0.00977348539145626
36-37	0.0015676768547699263
38-39	0.006981060993897328
40-41	0.004102904268343166
42-43	0.20562286495884605
44-45	0.18035632315110886
46-47	0.18689647502960216
48-49	0.18421427791089423
50-51	0.1828793030892542
52-53	0.18749660132556875
54-55	0.18621061640564032
56-57	0.19709862206103457
58-59	0.1929957177926914
60-61	0.1976742534061454
62-63	0.19571465733768298
64-65	0.1970741271101788
66-67	0.18688422755417425
68-69	0.18540228302739958
70-71	0.1702276609722438
72-73	0.1773434441958479
74-75	0.17681680275244865
76-77	0.17499192891369303
78-79	0.20140973341165186
80-81	0.21025241067058847
82-83	0.20770493578158733
84-85	0.20207109708475793
86-87	0.2188256434701115
88-89	0.21784584543588031
90-91	0.21619243625311513
92-93	0.22174054262194934
94-95	0.23297147758932452
96-97	0.23324092204873811
98-99	0.22295304268931046
100-101	0.23276327050705037
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4082474.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.06357836978023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.77401717309503	40.208404986576014
2	12.456443575648347	13.21966939770323
3	4.4362706204382745	7.062131812115396
4	2.140533782520465	4.543375284877471
5	1.2433040103579023	3.2987079895544307
6	0.8012311558412638	2.5509715338175503
7	0.5637830299624028	2.0941441495773483
8	0.4021998883104018	1.7073732234940693
9	0.3000336711452345	1.432877420014922
>10	1.7503796528832678	16.848599497147728
>50	0.09608783215047302	3.4603356830871026
>100	0.03409494095368157	2.7952220376211327
>500	9.724000522032663E-4	0.33369827018953835
>1k	6.482666408480064E-4	0.444488714224169
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.44949508557801E-5	0.0
9	0.0	0.0	0.0	2.44949508557801E-5	0.0
10-11	0.0	0.0	0.0	3.674242628367015E-5	0.0
12-13	0.0	0.0	0.0	4.89899017115602E-5	2.44949508557801E-5
14-15	0.0	0.0	0.0	4.89899017115602E-5	3.674242628367015E-5
16-17	0.0	0.0	0.0	4.89899017115602E-5	6.123737713945024E-5
18-19	0.0	0.0	0.0	4.89899017115602E-5	7.34848525673403E-5
20-21	0.0	1.224747542789005E-5	0.0	8.573232799523035E-5	7.34848525673403E-5
22-23	0.0	2.44949508557801E-5	0.0	1.3472222970679052E-4	1.1022727885101044E-4
24-25	0.0	2.44949508557801E-5	0.0	2.2045455770202088E-4	1.469697051346806E-4
26-27	0.0	2.44949508557801E-5	0.0	4.1641416454826167E-4	1.469697051346806E-4
28-29	0.0	2.44949508557801E-5	0.0	0.002155555675308649	1.469697051346806E-4
30-31	0.0	2.44949508557801E-5	0.0	0.009271338898912768	1.5921718056257064E-4
32-33	0.0	2.44949508557801E-5	0.0	0.022547602262745582	2.2045455770202088E-4
34-35	0.0	2.44949508557801E-5	0.0	0.04031868910861404	2.4494950855780096E-4
36-37	0.0	2.44949508557801E-5	0.0	0.06963914528298282	2.4494950855780096E-4
38-39	0.0	2.44949508557801E-5	0.0	0.12116427440811625	2.694444594135811E-4
40-41	0.0	2.44949508557801E-5	0.0	0.18099319187335916	2.694444594135811E-4
42-43	0.0	2.44949508557801E-5	0.0	0.24118953360143874	2.694444594135811E-4
44-45	0.0	3.674242628367015E-5	0.0	0.31464989121792325	2.8169193484147114E-4
46-47	0.0	4.89899017115602E-5	0.0	0.4021091132484861	3.306818365530313E-4
48-49	0.0	4.89899017115602E-5	0.0	0.49801909332429306	3.919192136924816E-4
50-51	0.0	4.89899017115602E-5	0.0	0.6054661952531725	3.919192136924816E-4
52-53	0.0	6.123737713945024E-5	0.0	0.7137338780357205	4.1641416454826167E-4
54-55	0.0	7.34848525673403E-5	0.0	0.8350818645752551	4.1641416454826167E-4
56-57	0.0	7.34848525673403E-5	0.0	0.9798837665591991	4.1641416454826167E-4
58-59	0.0	7.34848525673403E-5	0.0	1.1353899620671193	4.1641416454826167E-4
60-61	0.0	7.34848525673403E-5	0.0	1.3076262090095367	4.286616399761517E-4
62-63	0.0	7.34848525673403E-5	0.0	1.486059678518467	5.14393967971382E-4
64-65	0.0	7.34848525673403E-5	0.0	1.6814069115933132	5.388889188271622E-4
66-67	0.0	9.79798034231204E-5	0.0	1.9058541462848262	5.388889188271622E-4
68-69	0.0	9.79798034231204E-5	0.0	2.152861230714513	5.388889188271622E-4
70-71	0.0	1.1022727885101044E-4	0.0	2.4193785435008284	5.388889188271622E-4
72-73	0.0	1.2247475427890048E-4	0.0	2.7031647966404684	6.001262959666124E-4
74-75	0.0	1.2247475427890048E-4	0.0	2.9950098886116603	6.123737713945025E-4
76-77	0.0	1.2247475427890048E-4	0.0	3.318955613679352	6.123737713945025E-4
78-79	0.0	1.2247475427890048E-4	0.0	3.686931012910309	6.123737713945025E-4
80-81	0.0	1.2247475427890048E-4	0.0	4.078397070011959	6.368687222502825E-4
82-83	0.0	1.2247475427890048E-4	0.0	4.48819759782916	6.613636731060626E-4
84-85	0.0	1.2247475427890048E-4	0.0	4.914862899310565	6.613636731060626E-4
86-87	0.0	1.2247475427890048E-4	0.0	5.370885399392623	6.736111485339527E-4
88-89	0.0	1.2247475427890048E-4	0.0	5.86836560379809	6.858586239618427E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2730	0.0	42.587646	1
GTATCAA	4545	0.0	39.258545	1
TCAACGC	4975	0.0	34.629562	4
CAACGCA	5265	0.0	32.72014	5
ATCAACG	5465	0.0	31.433542	3
TATCAAC	5535	0.0	31.3801	2
AACGCAG	5610	0.0	30.870314	6
GTACATG	11015	0.0	28.520275	1
TACATGG	11365	0.0	26.682241	2
ACATGGG	11715	0.0	25.560066	3
ACGCAGA	7235	0.0	23.152351	7
CATGGGG	10475	0.0	22.382385	4
CGCAGAG	7535	0.0	21.476425	8
TACACCG	645	0.0	21.337578	5
GAGTACA	8075	0.0	20.451113	1
GTATAGG	950	0.0	19.980972	1
GTATATA	1055	0.0	19.34177	1
AGTACAT	8150	0.0	18.458336	2
ATGGGGG	6600	0.0	18.26403	5
ATACCGT	385	2.5465852E-10	17.253546	6
>>END_MODULE