FastQCFastQC Report
Mon 15 Aug 2016
SRR2921368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4082474
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27100.040.5107461
GTATCAA43250.035.9326441
GTACATG107200.029.0828271
TCAACGC52750.028.8807474
CAACGCA55150.027.7552095
ATCAACG55600.027.400353
TACATGG113900.026.7508242
TATCAAC59900.025.5918432
AACGCAG59900.025.552376
ACATGGG118700.025.1492963
CATGGGG100450.023.151124
ATGGGGG60700.020.2052925
ACGCAGA76150.019.5376017
TATACCG3203.929017E-1019.3120125
GAGTACA78550.018.9376341
CGCAGAG79100.018.3883088
TCTACAC23450.016.5957493
AGTACAT85000.016.5271762
GTATAGG11300.016.402641
TATACAG19800.016.0858385
Produced by FastQC (version 0.11.5)